Restriction map for
pFN21AE2739
(Linear) (Six-base) MAPSORT of: KIEE2739.seq Check: 4690 from: 1 to: 2016
KIEE2739.orf
With 182 enzymes: SgfI *
December 9, 2018 22:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 800 2016
Size: 800 1216
Acc65I G'GTAC_C
Cuts at: 0 953 2016
Size: 953 1063
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 1665 2016
Size: 979 686 351
Fragments arranged by size:
979 686 351
AfeI AGC'GCT
Cuts at: 0 394 898 2016
Size: 394 504 1118
Fragments arranged by size:
1118 504 394
AflIII A'CryG_T
Cuts at: 0 473 2016
Size: 473 1543
AleI CACnn'nnGTG
Cuts at: 0 1072 2016
Size: 1072 944
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1549 2016
Size: 1549 467
AlwNI CAG_nnn'CTG
Cuts at: 0 259 384 507 761 1830 2016
Size: 259 125 123 254 1069 186
Fragments arranged by size:
1069 259 254 186 125 123
ApaI G_GGCC'C
Cuts at: 0 45 1950 2016
Size: 45 1905 66
Fragments arranged by size:
1905 66 45
ApaLI G'TGCA_C
Cuts at: 0 1570 1638 2016
Size: 1570 68 378
Fragments arranged by size:
1570 378 68
AvaI C'yCGr_G
Cuts at: 0 858 1504 1521 1938 2016
Size: 858 646 17 417 78
Fragments arranged by size:
858 646 417 78 17
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 331 364 2016
Size: 331 33 1652
Fragments arranged by size:
1652 331 33
BamHI G'GATC_C
Cuts at: 0 732 2016
Size: 732 1284
BanI G'GyrC_C
Cuts at: 0 27 631 800 953 1038 1389 1450 1763
Size: 27 604 169 153 85 351 61 313
Cuts at: 1763 1806 2016
Size: 43 210
Fragments arranged by size:
604 351 313 210 169 153 85 61
43 27
BanII G_rGCy'C
Cuts at: 0 45 1796 1950 2016
Size: 45 1751 154 66
Fragments arranged by size:
1751 154 66 45
BbeI G_GCGC'C
Cuts at: 0 635 1454 2016
Size: 635 819 562
Fragments arranged by size:
819 635 562
BbsI GAAGACnn'nnnn_
Cuts at: 0 1401 1671 2016
Size: 1401 270 345
Fragments arranged by size:
1401 345 270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 283 317 378 412 695 729 2016
Size: 283 34 61 34 283 34 1287
Fragments arranged by size:
1287 283 283 61 34 34 34
BclI T'GATC_A
Cuts at: 0 998 2016
Size: 998 1018
BglI GCCn_nnn'nGGC
Cuts at: 0 49 2016
Size: 49 1967
BglII A'GATC_T
Cuts at: 0 461 2016
Size: 461 1555
Bme1580I G_kGCm'C
Cuts at: 0 45 1552 1574 1642 1768 1950 2016
Size: 45 1507 22 68 126 182 66
Fragments arranged by size:
1507 182 126 68 66 45 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 334 2016
Size: 334 1682
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 39 2016
Size: 39 1977
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 738 1939 2016
Size: 738 1201 77
Fragments arranged by size:
1201 738 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 688 1635 2016
Size: 688 947 381
Fragments arranged by size:
947 688 381
BsaHI Gr'CG_yC
Cuts at: 0 632 967 1451 2016
Size: 632 335 484 565
Fragments arranged by size:
632 565 484 335
BsaWI w'CCGG_w
Cuts at: 0 481 2016
Size: 481 1535
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 463 493 2016
Size: 463 30 1523
Fragments arranged by size:
1523 463 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 829 1606 1731 1906 2016
Size: 19 810 777 125 175 110
Fragments arranged by size:
810 777 175 125 110 19
BseYI C'CCAG_C
Cuts at: 0 1551 1943 2016
Size: 1551 392 73
Fragments arranged by size:
1551 392 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 894 913 1071 1351 1555 2016
Size: 153 741 19 158 280 204 461
Fragments arranged by size:
741 461 280 204 158 153 19
BsiEI CG_ry'CG
Cuts at: 0 405 1257 2016
Size: 405 852 759
Fragments arranged by size:
852 759 405
BsiHKAI G_wGCw'C
Cuts at: 0 11 434 1574 1642 2016
Size: 11 423 1140 68 374
Fragments arranged by size:
1140 423 374 68 11
BsmI GAATG_Cn'
Cuts at: 0 306 1007 2016
Size: 306 701 1009
Fragments arranged by size:
1009 701 306
BsmBI CGTCTCn'nnnn_
Cuts at: 0 861 2016
Size: 861 1155
Bsp1286I G_dGCh'C
Cuts at: 0 11 45 434 1552 1574 1642 1768 1796
Size: 11 34 389 1118 22 68 126 28
Cuts at: 1796 1950 2016
Size: 154 66
Fragments arranged by size:
1118 389 154 126 68 66 34 28
22 11
BspEI T'CCGG_A
Cuts at: 0 481 2016
Size: 481 1535
BspHI T'CATG_A
Cuts at: 0 1976 2016
Size: 1976 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 392 800 2016
Size: 392 408 1216
Fragments arranged by size:
1216 408 392
BsrDI GCAATG_nn'
Cuts at: 0 83 2016
Size: 83 1933
BsrFI r'CCGG_y
Cuts at: 0 195 1249 2016
Size: 195 1054 767
Fragments arranged by size:
1054 767 195
BsrGI T'GTAC_A
Cuts at: 0 446 593 1752 2016
Size: 446 147 1159 264
Fragments arranged by size:
1159 446 264 147
BssSI C'ACGA_G
Cuts at: 0 661 2016
Size: 661 1355
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1917 2016
Size: 1917 99
BstYI r'GATC_y
Cuts at: 0 461 732 816 2016
Size: 461 271 84 1200
Fragments arranged by size:
1200 461 271 84
Bsu36I CC'TnA_GG
Cuts at: 0 1043 2016
Size: 1043 973
BtgI C'CryG_G
Cuts at: 0 740 823 1099 1217 1325 1778 2016
Size: 740 83 276 118 108 453 238
Fragments arranged by size:
740 453 276 238 118 108 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 519 1074 2016
Size: 519 555 942
Fragments arranged by size:
942 555 519
BtsI GCAGTG_nn'
Cuts at: 0 237 1991 2016
Size: 237 1754 25
Fragments arranged by size:
1754 237 25
DraIII CAC_nnn'GTG
Cuts at: 0 243 666 807 2016
Size: 243 423 141 1209
Fragments arranged by size:
1209 423 243 141
DrdI GACnn_nn'nnGTC
Cuts at: 0 79 2016
Size: 79 1937
EaeI y'GGCC_r
Cuts at: 0 193 234 402 581 905 1160 1240 1988
Size: 193 41 168 179 324 255 80 748
Cuts at: 1988 2016
Size: 28
Fragments arranged by size:
748 324 255 193 179 168 80 41
28
EagI C'GGCC_G
Cuts at: 0 402 2016
Size: 402 1614
EarI CTCTTCn'nnn_
Cuts at: 0 217 313 691 907 1216 1360 1429 1822
Size: 217 96 378 216 309 144 69 393
Cuts at: 1822 2016
Size: 194
Fragments arranged by size:
393 378 309 217 216 194 144 96
69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 403 1392 1810 1865 2016
Size: 403 989 418 55 151
Fragments arranged by size:
989 418 403 151 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 738 979 1665 1939 2016
Size: 738 241 686 274 77
Fragments arranged by size:
738 686 274 241 77
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1885 2016
Size: 1885 131
EcoO109I rG'GnC_Cy
Cuts at: 0 41 51 203 1473 1713 1800 1935 2016
Size: 41 10 152 1270 240 87 135 81
Fragments arranged by size:
1270 240 152 135 87 81 41 10
FspI TGC'GCA
Cuts at: 0 388 2016
Size: 388 1628
HaeII r_GCGC'y
Cuts at: 0 266 396 635 900 1423 1454 2016
Size: 266 130 239 265 523 31 562
Fragments arranged by size:
562 523 266 265 239 130 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 48 80 463 495 801 833 836 868
Size: 48 32 383 32 306 32 3 32
Cuts at: 868 2016
Size: 1148
Fragments arranged by size:
1148 383 306 48 32 32 32 32
3
HincII GTy'rAC
Cuts at: 0 1959 2016
Size: 1959 57
KasI G'GCGC_C
Cuts at: 0 631 1450 2016
Size: 631 819 566
Fragments arranged by size:
819 631 566
KpnI G_GTAC'C
Cuts at: 0 957 2016
Size: 957 1059
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1109 2016
Size: 1109 907
MscI TGG'CCA
Cuts at: 0 236 2016
Size: 236 1780
MslI CAynn'nnrTG
Cuts at: 0 370 553 643 985 1072 1783 2016
Size: 370 183 90 342 87 711 233
Fragments arranged by size:
711 370 342 233 183 90 87
MspA1I CmG'CkG
Cuts at: 0 358 1101 1529 1900 2016
Size: 358 743 428 371 116
Fragments arranged by size:
743 428 371 358 116
NaeI GCC'GGC
Cuts at: 0 1251 2016
Size: 1251 765
NarI GG'CG_CC
Cuts at: 0 632 1451 2016
Size: 632 819 565
Fragments arranged by size:
819 632 565
NcoI C'CATG_G
Cuts at: 0 1217 1778 2016
Size: 1217 561 238
Fragments arranged by size:
1217 561 238
NgoMIV G'CCGG_C
Cuts at: 0 1249 2016
Size: 1249 767
NotI GC'GGCC_GC
Cuts at: 0 402 2016
Size: 402 1614
NspI r_CATG'y
Cuts at: 0 477 2016
Size: 477 1539
PciI A'CATG_T
Cuts at: 0 473 2016
Size: 473 1543
PflMI CCAn_nnn'nTGG
Cuts at: 0 243 666 1674 2016
Size: 243 423 1008 342
Fragments arranged by size:
1008 423 342 243
PpuMI rG'GwC_Cy
Cuts at: 0 1713 1935 2016
Size: 1713 222 81
Fragments arranged by size:
1713 222 81
PshAI GACnn'nnGTC
Cuts at: 0 616 2016
Size: 616 1400
PspOMI G'GGCC_C
Cuts at: 0 41 1946 2016
Size: 41 1905 70
Fragments arranged by size:
1905 70 41
PvuII CAG'CTG
Cuts at: 0 1529 2016
Size: 1529 487
SacII CC_GC'GG
Cuts at: 0 1102 2016
Size: 1102 914
SapI GCTCTTCn'nnn_
Cuts at: 0 313 907 1216 1360 1429 2016
Size: 313 594 309 144 69 587
Fragments arranged by size:
594 587 313 309 144 69
SexAI A'CCwGG_T
Cuts at: 0 881 2016
Size: 881 1135
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 49 2016
Size: 49 1967
SfoI GGC'GCC
Cuts at: 0 633 1452 2016
Size: 633 819 564
Fragments arranged by size:
819 633 564
SgrAI Cr'CCGG_yG
Cuts at: 0 1249 2016
Size: 1249 767
StyI C'CwwG_G
Cuts at: 0 1217 1778 2016
Size: 1217 561 238
Fragments arranged by size:
1217 561 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1275 1751 2016
Size: 1275 476 265
Fragments arranged by size:
1275 476 265
TatI w'GTAC_w
Cuts at: 0 446 593 1752 2016
Size: 446 147 1159 264
Fragments arranged by size:
1159 446 264 147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 808 840 2016
Size: 808 32 1176
Fragments arranged by size:
1176 808 32
Tth111I GACn'n_nGTC
Cuts at: 0 723 883 2016
Size: 723 160 1133
Fragments arranged by size:
1133 723 160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 465 978 2016
Size: 465 513 1038
Fragments arranged by size:
1038 513 465
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AleI AlfI AlwNI
ApaI ApaLI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BclI BglI BglII Bme1580I BmrI BplI
BpmI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PciI PflMI PpuMI PshAI PspOMI
PvuII SacII SapI SexAI SfiI SfoI SgrAI StyI
TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI AloI ApoI
AscI AseI AsiSI AvrII BbvCI BciVI BfrBI BlpI
BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsiWI BsrBI
BssHII BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI
EcoICRI EcoRI EcoRV FalI FseI FspAI HindIII HpaI
MfeI MluI NdeI NheI NruI NsiI PacI PasI
PfoI PmeI PmlI PpiI PsiI PspXI PsrI PstI
PvuI RsrII SacI SalI SanDI SbfI ScaI SfcI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI XbaI XhoI XmaI XmnI ZraI