(Linear) (Six-base) MAPSORT of: KIEE2736.seq  Check: 4987  from: 1  to: 711



KIEE2736.orf

 With 182 enzymes: SgfI * 

                        December 20, 2018 16:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     285     711 
   Size:        285     426
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     155     711 
   Size:        155     556
 
ApoI r'AATT_y

Cuts at:      0     612     711 
   Size:        612      99
 
AvrII C'CTAG_G

Cuts at:      0     312     711 
   Size:        312     399
 
BanI G'GyrC_C

Cuts at:      0     187     711 
   Size:        187     524
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     319     711 
   Size:        319     392
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     673     711 
   Size:        673      38
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     711 
   Size:        101     610
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     528     568     711 
   Size:        528      40     143

  Fragments arranged by size: 

                 528     143      40
 
BspHI T'CATG_A

Cuts at:      0     197     711 
   Size:        197     514
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     285     711 
   Size:        285     426
 
BsrDI GCAATG_nn'

Cuts at:      0     114     711 
   Size:        114     597
 
DraI TTT'AAA

Cuts at:      0     447     711 
   Size:        447     264
 
EarI CTCTTCn'nnn_

Cuts at:      0     434     711 
   Size:        434     277
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     155     711 
   Size:        155     556
 
HaeII r_GCGC'y

Cuts at:      0     389     711 
   Size:        389     322
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     130     162     711 
   Size:        130      32     549

  Fragments arranged by size: 

                 549     130      32
 
HindIII A'AGCT_T

Cuts at:      0     376     711 
   Size:        376     335
 
MslI CAynn'nnrTG

Cuts at:      0     196     427     711 
   Size:        196     231     284

  Fragments arranged by size: 

                 284     231     196
 
NspI r_CATG'y

Cuts at:      0     221     252     711 
   Size:        221      31     459

  Fragments arranged by size: 

                 459     221      31
 
PfoI T'CCnGG_A

Cuts at:      0     413     711 
   Size:        413     298
 
PstI C_TGCA'G

Cuts at:      0     296     711 
   Size:        296     415
 
SbfI CC_TGCA'GG

Cuts at:      0     296     711 
   Size:        296     415
 
SfcI C'TryA_G

Cuts at:      0     182     292     711 
   Size:        182     110     419

  Fragments arranged by size: 

                 419     182     110
 
SmlI C'TyrA_G

Cuts at:      0     116     711 
   Size:        116     595
 
SphI G_CATG'C

Cuts at:      0     221     252     711 
   Size:        221      31     459

  Fragments arranged by size: 

                 459     221      31
 
SspI AAT'ATT

Cuts at:      0      28     711 
   Size:         28     683
 
StyI C'CwwG_G

Cuts at:      0     312     711 
   Size:        312     399
 
XbaI T'CTAG_A

Cuts at:      0     522     711 
   Size:        522     189
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     550     711 
   Size:        550     161
 

 Enzymes that do cut:

     AarI     AcuI     ApoI    AvrII     BanI    BciVI     BmrI    BpuEI
     BsaI    BspHI    BspMI    BsrDI     DraI     EarI  Eco57MI    HaeII
    Hin4I  HindIII     MslI     NspI     PfoI     PstI     SbfI     SfcI
     SmlI     SphI     SspI     StyI     XbaI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XhoI     XmaI     XmnI     ZraI