Restriction map for
pFN21AE2734
(Linear) (Six-base) MAPSORT of: KIEE2734.seq Check: 8888 from: 1 to: 939
KIEE2734.orf
With 182 enzymes: SgfI *
September 25, 2018 15:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 141 741 939
Size: 141 600 198
Fragments arranged by size:
600 198 141
Acc65I G'GTAC_C
Cuts at: 0 911 939
Size: 911 28
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 939
Size: 178 761
AflIII A'CryG_T
Cuts at: 0 470 939
Size: 470 469
AleI CACnn'nnGTG
Cuts at: 0 886 939
Size: 886 53
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 169 939
Size: 169 770
AlwNI CAG_nnn'CTG
Cuts at: 0 156 939
Size: 156 783
ApaI G_GGCC'C
Cuts at: 0 312 939
Size: 312 627
AseI AT'TA_AT
Cuts at: 0 389 939
Size: 389 550
AvaI C'yCGr_G
Cuts at: 0 452 689 939
Size: 452 237 250
Fragments arranged by size:
452 250 237
BamHI G'GATC_C
Cuts at: 0 181 939
Size: 181 758
BanI G'GyrC_C
Cuts at: 0 911 939
Size: 911 28
BanII G_rGCy'C
Cuts at: 0 312 453 569 764 817 939
Size: 312 141 116 195 53 122
Fragments arranged by size:
312 195 141 122 116 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 75 939
Size: 75 864
BciVI GTATCCnnnnn_n'
Cuts at: 0 195 939
Size: 195 744
Bme1580I G_kGCm'C
Cuts at: 0 312 939
Size: 312 627
BmrI ACTGGGnnnn_n'
Cuts at: 0 264 335 939
Size: 264 71 604
Fragments arranged by size:
604 264 71
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 561 695 939
Size: 561 134 244
Fragments arranged by size:
561 244 134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 939
Size: 664 275
Bpu10I CC'TnA_GC
Cuts at: 0 904 939
Size: 904 35
BsaI GGTCTCn'nnnn_
Cuts at: 0 679 939
Size: 679 260
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 505 535 939
Size: 505 30 404
Fragments arranged by size:
505 404 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 190 844 939
Size: 190 654 95
Fragments arranged by size:
654 190 95
BsiHKAI G_wGCw'C
Cuts at: 0 569 939
Size: 569 370
BsmBI CGTCTCn'nnnn_
Cuts at: 0 525 939
Size: 525 414
Bsp1286I G_dGCh'C
Cuts at: 0 312 453 569 764 817 939
Size: 312 141 116 195 53 122
Fragments arranged by size:
312 195 141 122 116 53
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 125 141 741 939
Size: 125 16 600 198
Fragments arranged by size:
600 198 125 16
BsrFI r'CCGG_y
Cuts at: 0 908 939
Size: 908 31
BstXI CCAn_nnnn'nTGG
Cuts at: 0 132 231 939
Size: 132 99 708
Fragments arranged by size:
708 132 99
BstYI r'GATC_y
Cuts at: 0 181 939
Size: 181 758
Bsu36I CC'TnA_GG
Cuts at: 0 680 939
Size: 680 259
BtgI C'CryG_G
Cuts at: 0 437 939
Size: 437 502
EaeI y'GGCC_r
Cuts at: 0 122 939
Size: 122 817
EcoICRI GAG'CTC
Cuts at: 0 567 939
Size: 567 372
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 664 939
Size: 178 486 275
Fragments arranged by size:
486 275 178
EcoO109I rG'GnC_Cy
Cuts at: 0 309 939
Size: 309 630
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 99 390 439 939
Size: 99 291 49 500
Fragments arranged by size:
500 291 99 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 764 796 939
Size: 166 32 566 32 143
Fragments arranged by size:
566 166 143 32 32
HincII GTy'rAC
Cuts at: 0 468 939
Size: 468 471
HindIII A'AGCT_T
Cuts at: 0 661 939
Size: 661 278
KpnI G_GTAC'C
Cuts at: 0 915 939
Size: 915 24
MscI TGG'CCA
Cuts at: 0 124 939
Size: 124 815
MslI CAynn'nnrTG
Cuts at: 0 151 886 939
Size: 151 735 53
Fragments arranged by size:
735 151 53
MspA1I CmG'CkG
Cuts at: 0 132 198 928 939
Size: 132 66 730 11
Fragments arranged by size:
730 132 66 11
NcoI C'CATG_G
Cuts at: 0 437 939
Size: 437 502
NspI r_CATG'y
Cuts at: 0 150 432 474 657 799 939
Size: 150 282 42 183 142 140
Fragments arranged by size:
282 183 150 142 140 42
PciI A'CATG_T
Cuts at: 0 470 939
Size: 470 469
PflMI CCAn_nnn'nTGG
Cuts at: 0 356 939
Size: 356 583
PfoI T'CCnGG_A
Cuts at: 0 808 939
Size: 808 131
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 287 319 939
Size: 287 32 620
Fragments arranged by size:
620 287 32
PspOMI G'GGCC_C
Cuts at: 0 308 939
Size: 308 631
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 22 54 939
Size: 22 32 885
Fragments arranged by size:
885 32 22
PstI C_TGCA'G
Cuts at: 0 221 939
Size: 221 718
PvuII CAG'CTG
Cuts at: 0 132 939
Size: 132 807
SacI G_AGCT'C
Cuts at: 0 569 939
Size: 569 370
SexAI A'CCwGG_T
Cuts at: 0 398 939
Size: 398 541
SfcI C'TryA_G
Cuts at: 0 217 939
Size: 217 722
SmaI CCC'GGG
Cuts at: 0 454 691 939
Size: 454 237 248
Fragments arranged by size:
454 248 237
SphI G_CATG'C
Cuts at: 0 150 657 799 939
Size: 150 507 142 140
Fragments arranged by size:
507 150 142 140
StuI AGG'CCT
Cuts at: 0 302 541 939
Size: 302 239 398
Fragments arranged by size:
398 302 239
StyI C'CwwG_G
Cuts at: 0 365 437 919 939
Size: 365 72 482 20
Fragments arranged by size:
482 365 72 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 264 939
Size: 264 675
Tth111I GACn'n_nGTC
Cuts at: 0 934 939
Size: 934 5
XmaI C'CCGG_G
Cuts at: 0 452 689 939
Size: 452 237 250
Fragments arranged by size:
452 250 237
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI AlwNI ApaI
AseI AvaI BamHI BanI BanII BbsI BciVI Bme1580I
BmrI BplI BpmI Bpu10I BsaI BsaXI BseRI BsiHKAI
BsmBI Bsp1286I BspMI BsrFI BstXI BstYI Bsu36I BtgI
EaeI EcoICRI Eco57MI EcoO109I FalI Hin4I HincII HindIII
KpnI MscI MslI MspA1I NcoI NspI PciI PflMI
PfoI PpiI PspOMI PsrI PstI PvuII SacI SexAI
SfcI SmaI SphI StuI StyI TaqII Tth111I XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AloI
ApaLI ApoI AscI AsiSI AvrII BaeI BbeI BbvCI
BcgI BclI BfrBI BglI BglII BlpI BmgBI BmtI
BpuEI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PmlI PpuMI
PshAI PsiI PspXI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI SspI SwaI TatI TstI XbaI
XcmI XhoI XmnI ZraI