(Linear) (Six-base) MAPSORT of: KIEE2731.seq  Check: 8682  from: 1  to: 972



KIEE2731.orf

 With 182 enzymes: SgfI * 

                        August 17, 2018 17:41  ..

AccI GT'mk_AC

Cuts at:      0     766     972 
   Size:        766     206
 
Acc65I G'GTAC_C

Cuts at:      0     791     972 
   Size:        791     181
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     972 
   Size:         29     943
 
ApoI r'AATT_y

Cuts at:      0     268     419     972 
   Size:        268     151     553

  Fragments arranged by size: 

                 553     268     151
 
AseI AT'TA_AT

Cuts at:      0     931     972 
   Size:        931      41
 
BanI G'GyrC_C

Cuts at:      0     791     972 
   Size:        791     181
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      82     972 
   Size:         82     890
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     874     972 
   Size:        874      98
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     255     823     972 
   Size:        255     568     149

  Fragments arranged by size: 

                 568     255     149
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     359     972 
   Size:        359     613
 
Bpu10I CC'TnA_GC

Cuts at:      0     116     972 
   Size:        116     856
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     332     362     972 
   Size:        332      30     610

  Fragments arranged by size: 

                 610     332      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     143     972 
   Size:        143     829
 
BsiEI CG_ry'CG

Cuts at:      0     763     972 
   Size:        763     209
 
BstZ17I GTA'TAC

Cuts at:      0     767     972 
   Size:        767     205
 
EaeI y'GGCC_r

Cuts at:      0     760     972 
   Size:        760     212
 
EagI C'GGCC_G

Cuts at:      0     760     972 
   Size:        760     212
 
EarI CTCTTCn'nnn_

Cuts at:      0     883     972 
   Size:        883      89
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     359     972 
   Size:         29     330     613

  Fragments arranged by size: 

                 613     330      29
 
EcoRV GAT'ATC

Cuts at:      0     276     972 
   Size:        276     696
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     405     669     972 
   Size:        405     264     303

  Fragments arranged by size: 

                 405     303     264
 
KpnI G_GTAC'C

Cuts at:      0     795     972 
   Size:        795     177
 
MslI CAynn'nnrTG

Cuts at:      0     584     972 
   Size:        584     388
 
MspA1I CmG'CkG

Cuts at:      0     108     972 
   Size:        108     864
 
SspI AAT'ATT

Cuts at:      0      42     972 
   Size:         42     930
 
StuI AGG'CCT

Cuts at:      0     195     972 
   Size:        195     777
 
StyI C'CwwG_G

Cuts at:      0     867     972 
   Size:        867     105
 
XmnI GAAnn'nnTTC

Cuts at:      0      42     389     972 
   Size:         42     347     583

  Fragments arranged by size: 

                 583     347      42
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     ApoI     AseI     BanI     BbsI    BciVI
     BmrI     BpmI   Bpu10I    BsaXI    BseRI    BsiEI  BstZ17I     EaeI
     EagI     EarI  Eco57MI    EcoRV     FalI     KpnI     MslI   MspA1I
     SspI     StuI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI