(Linear) (Six-base) MAPSORT of: KIEE2726.seq  Check: 3820  from: 1  to: 1266



KIEE2726.orf

 With 182 enzymes: SgfI * 

                        July 3, 2018 14:52  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     799    1266 
   Size:        799     467
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1207    1266 
   Size:       1207      59
 
AleI CACnn'nnGTG

Cuts at:      0     271    1266 
   Size:        271     995
 
AlwNI CAG_nnn'CTG

Cuts at:      0     410    1116    1266 
   Size:        410     706     150

  Fragments arranged by size: 

                 706     410     150
 
ApoI r'AATT_y

Cuts at:      0     530    1003    1266 
   Size:        530     473     263

  Fragments arranged by size: 

                 530     473     263
 
AseI AT'TA_AT

Cuts at:      0     149    1266 
   Size:        149    1117
 
AvaI C'yCGr_G

Cuts at:      0     125    1266 
   Size:        125    1141
 
BamHI G'GATC_C

Cuts at:      0      54     814     863    1266 
   Size:         54     760      49     403

  Fragments arranged by size: 

                 760     403      54      49
 
BanI G'GyrC_C

Cuts at:      0      39     577    1266 
   Size:         39     538     689

  Fragments arranged by size: 

                 689     538      39
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     172    1195    1266 
   Size:        172    1023      71

  Fragments arranged by size: 

                1023     172      71
 
BclI T'GATC_A

Cuts at:      0     206    1266 
   Size:        206    1060
 
Bme1580I G_kGCm'C

Cuts at:      0     747    1266 
   Size:        747     519
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     420     535    1266 
   Size:        420     115     731

  Fragments arranged by size: 

                 731     420     115
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     934    1266 
   Size:        934     332
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     578     759    1266 
   Size:        578     181     507

  Fragments arranged by size: 

                 578     507     181
 
Bpu10I CC'TnA_GC

Cuts at:      0     898    1266 
   Size:        898     368
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     177    1266 
   Size:        177    1089
 
BsaBI GATnn'nnATC

Cuts at:      0     652    1266 
   Size:        652     614
 
BsaHI Gr'CG_yC

Cuts at:      0     752    1219    1266 
   Size:        752     467      47

  Fragments arranged by size: 

                 752     467      47
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     206     236    1266 
   Size:        206      30    1030

  Fragments arranged by size: 

                1030     206      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     969    1266 
   Size:        969     297
 
Bsp1286I G_dGCh'C

Cuts at:      0     747     969    1266 
   Size:        747     222     297

  Fragments arranged by size: 

                 747     297     222
 
BspHI T'CATG_A

Cuts at:      0     246    1266 
   Size:        246    1020
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     799    1266 
   Size:        799     467
 
BsrBI CCG'CTC

Cuts at:      0      70    1266 
   Size:         70    1196
 
BssSI C'ACGA_G

Cuts at:      0      11    1266 
   Size:         11    1255
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     364     973    1266 
   Size:        364     609     293

  Fragments arranged by size: 

                 609     364     293
 
BstYI r'GATC_y

Cuts at:      0      54     814     863    1266 
   Size:         54     760      49     403

  Fragments arranged by size: 

                 760     403      54      49
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      99     134    1266 
   Size:         99      35    1132

  Fragments arranged by size: 

                1132      99      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1228    1266 
   Size:       1228      38
 
EarI CTCTTCn'nnn_

Cuts at:      0     941    1232    1266 
   Size:        941     291      34

  Fragments arranged by size: 

                 941     291      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     578     759    1207    1266 
   Size:        578     181     448      59

  Fragments arranged by size: 

                 578     448     181      59
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1133    1266 
   Size:       1133     133
 
EcoRI G'AATT_C

Cuts at:      0     530    1003    1266 
   Size:        530     473     263

  Fragments arranged by size: 

                 530     473     263
 
EcoRV GAT'ATC

Cuts at:      0     929    1266 
   Size:        929     337
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      46      78    1188    1220    1266 
   Size:         46      32    1110      32      46

  Fragments arranged by size: 

                1110      46      46      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     543    1266 
   Size:        543     723
 
MslI CAynn'nnrTG

Cuts at:      0     271     308    1266 
   Size:        271      37     958

  Fragments arranged by size: 

                 958     271      37
 
MspA1I CmG'CkG

Cuts at:      0    1056    1266 
   Size:       1056     210
 
PpuMI rG'GwC_Cy

Cuts at:      0    1133    1266 
   Size:       1133     133
 
SmaI CCC'GGG

Cuts at:      0     127    1266 
   Size:        127    1139
 
SspI AAT'ATT

Cuts at:      0     503    1266 
   Size:        503     763
 
TatI w'GTAC_w

Cuts at:      0     690    1266 
   Size:        690     576
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     206     238    1266 
   Size:        206      32    1028

  Fragments arranged by size: 

                1028     206      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1137    1266 
   Size:       1137     129
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     183     762    1266 
   Size:        183     579     504

  Fragments arranged by size: 

                 579     504     183
 
XmaI C'CCGG_G

Cuts at:      0     125    1266 
   Size:        125    1141
 
XmnI GAAnn'nnTTC

Cuts at:      0     338    1266 
   Size:        338     928
 

 Enzymes that do cut:

     AarI     AcuI     AleI    AlwNI     ApoI     AseI     AvaI    BamHI
     BanI     BbsI     BclI Bme1580I     BmrI     BplI     BpmI   Bpu10I
     BsaI    BsaBI    BsaHI    BsaXI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrBI    BssSI   BstAPI    BstYI    CspCI   DraIII     EarI  Eco57MI
 EcoO109I    EcoRI    EcoRV    Hin4I     MmeI     MslI   MspA1I    PpuMI
     SmaI     SspI     TatI     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII
     BaeI    BanII     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI    BpuEI    BsaAI    BsaWI    BseRI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrDI
    BsrFI    BsrGI   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     XbaI     XhoI     ZraI