(Linear) (Six-base) MAPSORT of: KIEE2724.seq  Check: 1576  from: 1  to: 597



KIEE2724.orf

 With 182 enzymes: SgfI * 

                        June 13, 2018 19:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     539     597 
   Size:        539      58
 
AlwNI CAG_nnn'CTG

Cuts at:      0     127     597 
   Size:        127     470
 
ApoI r'AATT_y

Cuts at:      0      63     272     597 
   Size:         63     209     325

  Fragments arranged by size: 

                 325     209      63
 
BbvCI CC'TCA_GC

Cuts at:      0     266     597 
   Size:        266     331
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     415     597 
   Size:        415     182
 
BglII A'GATC_T

Cuts at:      0     549     597 
   Size:        549      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     392     597 
   Size:        392     205
 
Bpu10I CC'TnA_GC

Cuts at:      0     266     597 
   Size:        266     331
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     372     402     597 
   Size:        372      30     195

  Fragments arranged by size: 

                 372     195      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     396     399     597 
   Size:        396       3     198

  Fragments arranged by size: 

                 396     198       3
 
BstYI r'GATC_y

Cuts at:      0     549     597 
   Size:        549      48
 
BtsI GCAGTG_nn'

Cuts at:      0      96     597 
   Size:         96     501
 
DraIII CAC_nnn'GTG

Cuts at:      0     240     597 
   Size:        240     357
 
EaeI y'GGCC_r

Cuts at:      0     432     597 
   Size:        432     165
 
EarI CTCTTCn'nnn_

Cuts at:      0     515     597 
   Size:        515      82
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     392     539     597 
   Size:        392     147      58

  Fragments arranged by size: 

                 392     147      58
 
EcoO109I rG'GnC_Cy

Cuts at:      0     525     597 
   Size:        525      72
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     316     597 
   Size:        316     281
 
MscI TGG'CCA

Cuts at:      0     434     597 
   Size:        434     163
 
NdeI CA'TA_TG

Cuts at:      0     363     597 
   Size:        363     234
 
PfoI T'CCnGG_A

Cuts at:      0     417     597 
   Size:        417     180
 
PpuMI rG'GwC_Cy

Cuts at:      0     525     597 
   Size:        525      72
 
PsiI TTA'TAA

Cuts at:      0     314     597 
   Size:        314     283
 
TatI w'GTAC_w

Cuts at:      0     388     458     597 
   Size:        388      70     139

  Fragments arranged by size: 

                 388     139      70
 
XmnI GAAnn'nnTTC

Cuts at:      0     296     597 
   Size:        296     301
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI    BbvCI    BciVI    BglII     BpmI   Bpu10I
    BsaXI    BseRI    BstYI     BtsI   DraIII     EaeI     EarI  Eco57MI
 EcoO109I     FalI     MscI     NdeI     PfoI    PpuMI     PsiI     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI