(Linear) (Six-base) MAPSORT of: KIEE2714.seq  Check: 9238  from: 1  to: 663



KIEE2714.orf

 With 182 enzymes: SgfI * 

                        January 30, 2018 10:51  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     663 
   Size:        119     544
 
AflIII A'CryG_T

Cuts at:      0     326     527     663 
   Size:        326     201     136

  Fragments arranged by size: 

                 326     201     136
 
ApoI r'AATT_y

Cuts at:      0     445     663 
   Size:        445     218
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     267     300     663 
   Size:        267      33     363

  Fragments arranged by size: 

                 363     267      33
 
BamHI G'GATC_C

Cuts at:      0     141     663 
   Size:        141     522
 
BanII G_rGCy'C

Cuts at:      0     186     663 
   Size:        186     477
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     615     663 
   Size:        615      48
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     146     176     663 
   Size:        146      30     487

  Fragments arranged by size: 

                 487     146      30
 
BseYI C'CCAG_C

Cuts at:      0      68     642     663 
   Size:         68     574      21

  Fragments arranged by size: 

                 574      68      21
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      15     388     663 
   Size:         15     373     275

  Fragments arranged by size: 

                 373     275      15
 
Bsp1286I G_dGCh'C

Cuts at:      0     186     663 
   Size:        186     477
 
BspHI T'CATG_A

Cuts at:      0     591     663 
   Size:        591      72
 
BstEII G'GTnAC_C

Cuts at:      0     267     663 
   Size:        267     396
 
BstYI r'GATC_y

Cuts at:      0     141     557     663 
   Size:        141     416     106

  Fragments arranged by size: 

                 416     141     106
 
BtgI C'CryG_G

Cuts at:      0     454     663 
   Size:        454     209
 
BtsI GCAGTG_nn'

Cuts at:      0     364     663 
   Size:        364     299
 
DraI TTT'AAA

Cuts at:      0     258     663 
   Size:        258     405
 
EarI CTCTTCn'nnn_

Cuts at:      0     424     663 
   Size:        424     239
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     663 
   Size:        119     544
 
EcoO109I rG'GnC_Cy

Cuts at:      0      33     513     663 
   Size:         33     480     150

  Fragments arranged by size: 

                 480     150      33
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     549     581     663 
   Size:        549      32      82

  Fragments arranged by size: 

                 549      82      32
 
MspA1I CmG'CkG

Cuts at:      0      72     631     663 
   Size:         72     559      32

  Fragments arranged by size: 

                 559      72      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      33     663 
   Size:         33     630
 
PvuII CAG'CTG

Cuts at:      0     631     663 
   Size:        631      32
 
SanDI GG'GwC_CC

Cuts at:      0      33     663 
   Size:         33     630
 
StyI C'CwwG_G

Cuts at:      0     263     663 
   Size:        263     400
 
SwaI ATTT'AAAT

Cuts at:      0     258     663 
   Size:        258     405
 
TatI w'GTAC_w

Cuts at:      0     524     663 
   Size:        524     139
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     146     178     663 
   Size:        146      32     485

  Fragments arranged by size: 

                 485     146      32
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     BaeI    BamHI    BanII     BmrI    BsaXI
    BseYI    BsmBI Bsp1286I    BspHI   BstEII    BstYI     BtgI     BtsI
     DraI     EarI  Eco57MI EcoO109I    Hin4I   MspA1I    PpuMI    PvuII
    SanDI     StyI     SwaI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI    TaqII
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI