(Linear) (Six-base) MAPSORT of: KIEE2707.seq  Check: 4075  from: 1  to: 711



KIEE2707.orf

 With 182 enzymes: SgfI * 

                        December 15, 2017 09:57  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     508     621     711 
   Size:        281     227     113      90

  Fragments arranged by size: 

                 281     227     113      90
 
AhdI GACnn_n'nnGTC

Cuts at:      0     189     711 
   Size:        189     522
 
AlwNI CAG_nnn'CTG

Cuts at:      0      21     711 
   Size:         21     690
 
ApoI r'AATT_y

Cuts at:      0      49     711 
   Size:         49     662
 
AseI AT'TA_AT

Cuts at:      0     340     711 
   Size:        340     371
 
BanI G'GyrC_C

Cuts at:      0     560     711 
   Size:        560     151
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     139     711 
   Size:        139     572
 
BfrBI ATG'CAT

Cuts at:      0     433     711 
   Size:        433     278
 
BsmI GAATG_Cn'

Cuts at:      0     435     711 
   Size:        435     276
 
BsrFI r'CCGG_y

Cuts at:      0     149     711 
   Size:        149     562
 
BssSI C'ACGA_G

Cuts at:      0      10     711 
   Size:         10     701
 
BstYI r'GATC_y

Cuts at:      0      17     711 
   Size:         17     694
 
DraIII CAC_nnn'GTG

Cuts at:      0     469     711 
   Size:        469     242
 
EarI CTCTTCn'nnn_

Cuts at:      0     503     665     711 
   Size:        503     162      46

  Fragments arranged by size: 

                 503     162      46
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      23     711 
   Size:         23     688
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     508     621     711 
   Size:        281     227     113      90

  Fragments arranged by size: 

                 281     227     113      90
 
EcoO109I rG'GnC_Cy

Cuts at:      0      58     711 
   Size:         58     653
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     175     207     632     664     711 
   Size:        175      32     425      32      47

  Fragments arranged by size: 

                 425     175      47      32      32
 
HincII GTy'rAC

Cuts at:      0     194     711 
   Size:        194     517
 
NsiI A_TGCA'T

Cuts at:      0     435     711 
   Size:        435     276
 
PasI CC'CwG_GG

Cuts at:      0     187     711 
   Size:        187     524
 
SfcI C'TryA_G

Cuts at:      0     137     217     711 
   Size:        137      80     494

  Fragments arranged by size: 

                 494     137      80
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     298     330     711 
   Size:        298      32     381

  Fragments arranged by size: 

                 381     298      32
 

 Enzymes that do cut:

     AcuI     AhdI    AlwNI     ApoI     AseI     BanI     BbsI    BfrBI
     BsmI    BsrFI    BssSI    BstYI   DraIII     EarI     EciI  Eco57MI
 EcoO109I    Hin4I   HincII     NsiI     PasI     SfcI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI