Restriction map for
pFN21AE2703
(Linear) (Six-base) MAPSORT of: KIEE2703.seq Check: 1664 from: 1 to: 2595
KIEE2703.orf
With 182 enzymes: SgfI *
September 21, 2017 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1730 2595
Size: 1730 865
AccI GT'mk_AC
Cuts at: 0 308 2595
Size: 308 2287
AclI AA'CG_TT
Cuts at: 0 1031 1040 2595
Size: 1031 9 1555
Fragments arranged by size:
1555 1031 9
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1822 1837 2595
Size: 1822 15 758
Fragments arranged by size:
1822 758 15
AflIII A'CryG_T
Cuts at: 0 2477 2595
Size: 2477 118
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 661 693 2595
Size: 661 32 1902
Fragments arranged by size:
1902 661 32
AlwNI CAG_nnn'CTG
Cuts at: 0 283 1685 2595
Size: 283 1402 910
Fragments arranged by size:
1402 910 283
ApaI G_GGCC'C
Cuts at: 0 455 2595
Size: 455 2140
ApoI r'AATT_y
Cuts at: 0 232 719 1158 1266 1992 2373 2595
Size: 232 487 439 108 726 381 222
Fragments arranged by size:
726 487 439 381 232 222 108
AseI AT'TA_AT
Cuts at: 0 1760 2595
Size: 1760 835
AvaI C'yCGr_G
Cuts at: 0 1412 2595
Size: 1412 1183
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2318 2351 2595
Size: 2318 33 244
Fragments arranged by size:
2318 244 33
BanII G_rGCy'C
Cuts at: 0 455 2595
Size: 455 2140
BbsI GAAGACnn'nnnn_
Cuts at: 0 1189 1434 1613 2595
Size: 1189 245 179 982
Fragments arranged by size:
1189 982 245 179
BciVI GTATCCnnnnn_n'
Cuts at: 0 1578 2595
Size: 1578 1017
BclI T'GATC_A
Cuts at: 0 1493 2595
Size: 1493 1102
BglII A'GATC_T
Cuts at: 0 684 2595
Size: 684 1911
BlpI GC'TnA_GC
Cuts at: 0 859 2595
Size: 859 1736
Bme1580I G_kGCm'C
Cuts at: 0 455 2595
Size: 455 2140
BmgBI CAC'GTC
Cuts at: 0 2478 2595
Size: 2478 117
BmrI ACTGGGnnnn_n'
Cuts at: 0 645 1620 2595
Size: 645 975 975
Fragments arranged by size:
975 975 645
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 2595
Size: 608 1987
BsaI GGTCTCn'nnnn_
Cuts at: 0 118 729 787 2595
Size: 118 611 58 1808
Fragments arranged by size:
1808 611 118 58
BsaHI Gr'CG_yC
Cuts at: 0 1439 2595
Size: 1439 1156
BsaWI w'CCGG_w
Cuts at: 0 1432 2595
Size: 1432 1163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 66 96 2464 2494 2595
Size: 66 30 2368 30 101
Fragments arranged by size:
2368 101 66 30 30
BseYI C'CCAG_C
Cuts at: 0 30 1305 2595
Size: 30 1275 1290
Fragments arranged by size:
1290 1275 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1853 2595
Size: 1853 742
BsiHKAI G_wGCw'C
Cuts at: 0 2080 2595
Size: 2080 515
BsmI GAATG_Cn'
Cuts at: 0 158 1222 2595
Size: 158 1064 1373
Fragments arranged by size:
1373 1064 158
Bsp1286I G_dGCh'C
Cuts at: 0 455 2080 2595
Size: 455 1625 515
Fragments arranged by size:
1625 515 455
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 952 1129 1730 2595
Size: 952 177 601 865
Fragments arranged by size:
952 865 601 177
BsrBI CCG'CTC
Cuts at: 0 2320 2595
Size: 2320 275
BssSI C'ACGA_G
Cuts at: 0 1557 2595
Size: 1557 1038
BstXI CCAn_nnnn'nTGG
Cuts at: 0 145 2595
Size: 145 2450
BstYI r'GATC_y
Cuts at: 0 684 2595
Size: 684 1911
Bsu36I CC'TnA_GG
Cuts at: 0 86 460 2595
Size: 86 374 2135
Fragments arranged by size:
2135 374 86
BtgI C'CryG_G
Cuts at: 0 773 1010 2595
Size: 773 237 1585
Fragments arranged by size:
1585 773 237
BtsI GCAGTG_nn'
Cuts at: 0 1791 2269 2595
Size: 1791 478 326
Fragments arranged by size:
1791 478 326
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 399 434 2595
Size: 399 35 2161
Fragments arranged by size:
2161 399 35
EaeI y'GGCC_r
Cuts at: 0 2312 2595
Size: 2312 283
EarI CTCTTCn'nnn_
Cuts at: 0 826 1277 1721 2595
Size: 826 451 444 874
Fragments arranged by size:
874 826 451 444
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 731 2595
Size: 731 1864
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 1822 1837 2595
Size: 608 1214 15 758
Fragments arranged by size:
1214 758 608 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 459 2595
Size: 459 2136
EcoO109I rG'GnC_Cy
Cuts at: 0 452 2595
Size: 452 2143
EcoRI G'AATT_C
Cuts at: 0 719 1158 2595
Size: 719 439 1437
Fragments arranged by size:
1437 719 439
EcoRV GAT'ATC
Cuts at: 0 1176 2595
Size: 1176 1419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 728 754 760 786 1201 1233 1406 1438
Size: 728 26 6 26 415 32 173 32
Cuts at: 1438 2595
Size: 1157
Fragments arranged by size:
1157 728 415 173 32 32 26 26
6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 788 1095 1434 1440 1451 2108 2595
Size: 550 238 307 339 6 11 657 487
Fragments arranged by size:
657 550 487 339 307 238 11 6
MscI TGG'CCA
Cuts at: 0 2314 2595
Size: 2314 281
MslI CAynn'nnrTG
Cuts at: 0 1299 2552 2595
Size: 1299 1253 43
Fragments arranged by size:
1299 1253 43
MspA1I CmG'CkG
Cuts at: 0 975 2595
Size: 975 1620
NcoI C'CATG_G
Cuts at: 0 773 2595
Size: 773 1822
NdeI CA'TA_TG
Cuts at: 0 249 1953 2595
Size: 249 1704 642
Fragments arranged by size:
1704 642 249
NspI r_CATG'y
Cuts at: 0 1399 2595
Size: 1399 1196
PspOMI G'GGCC_C
Cuts at: 0 451 2595
Size: 451 2144
PstI C_TGCA'G
Cuts at: 0 1727 2264 2595
Size: 1727 537 331
Fragments arranged by size:
1727 537 331
PvuII CAG'CTG
Cuts at: 0 975 2595
Size: 975 1620
SapI GCTCTTCn'nnn_
Cuts at: 0 826 2595
Size: 826 1769
ScaI AGT'ACT
Cuts at: 0 2452 2595
Size: 2452 143
SexAI A'CCwGG_T
Cuts at: 0 542 2595
Size: 542 2053
SfcI C'TryA_G
Cuts at: 0 1723 2060 2260 2595
Size: 1723 337 200 335
Fragments arranged by size:
1723 337 335 200
SpeI A'CTAG_T
Cuts at: 0 171 2595
Size: 171 2424
SphI G_CATG'C
Cuts at: 0 1399 2595
Size: 1399 1196
StyI C'CwwG_G
Cuts at: 0 665 773 2595
Size: 665 108 1822
Fragments arranged by size:
1822 665 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 743 1297 1819 2595
Size: 743 554 522 776
Fragments arranged by size:
776 743 554 522
TatI w'GTAC_w
Cuts at: 0 653 850 1110 2450 2595
Size: 653 197 260 1340 145
Fragments arranged by size:
1340 653 260 197 145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 66 98 2595
Size: 66 32 2497
Fragments arranged by size:
2497 66 32
XbaI T'CTAG_A
Cuts at: 0 1959 2322 2595
Size: 1959 363 273
Fragments arranged by size:
1959 363 273
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 804 2511 2595
Size: 804 1707 84
Fragments arranged by size:
1707 804 84
XmnI GAAnn'nnTTC
Cuts at: 0 1732 2466 2595
Size: 1732 734 129
Fragments arranged by size:
1732 734 129
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AloI AlwNI ApaI
ApoI AseI AvaI BaeI BanII BbsI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaHI
BsaWI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BssSI BstXI BstYI Bsu36I BtgI BtsI CspCI
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
Hin4I MmeI MscI MslI MspA1I NcoI NdeI NspI
PspOMI PstI PvuII SapI ScaI SexAI SfcI SpeI
SphI StyI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AhdI AleI AlfI
ApaLI AscI AsiSI AvrII BamHI BanI BbeI BbvCI
BcgI BfrBI BglI BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BseRI BsiEI BsiWI BsmBI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgZI
ClaI DraI DraIII DrdI EagI EcoICRI FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SrfI SspI StuI SwaI
Tth111I XhoI XmaI ZraI