Restriction map for
pFN21AE2702
(Linear) (Six-base) MAPSORT of: KIEE2702.seq Check: 5678 from: 1 to: 1287
KIEE2702.orf
With 182 enzymes: SgfI *
September 11, 2017 11:55 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 80 1284 1287
Size: 80 1204 3
Fragments arranged by size:
1204 80 3
AseI AT'TA_AT
Cuts at: 0 232 1287
Size: 232 1055
BamHI G'GATC_C
Cuts at: 0 403 1287
Size: 403 884
BanI G'GyrC_C
Cuts at: 0 339 1287
Size: 339 948
BciVI GTATCCnnnnn_n'
Cuts at: 0 599 1287
Size: 599 688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 910 1287
Size: 102 808 377
Fragments arranged by size:
808 377 102
Bpu10I CC'TnA_GC
Cuts at: 0 1219 1287
Size: 1219 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 787 1061 1287
Size: 158 629 274 226
Fragments arranged by size:
629 274 226 158
BsaI GGTCTCn'nnnn_
Cuts at: 0 299 1287
Size: 299 988
BsaHI Gr'CG_yC
Cuts at: 0 47 1287
Size: 47 1240
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 75 105 589 619 695 725 1287
Size: 75 30 484 30 76 30 562
Fragments arranged by size:
562 484 76 75 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 703 1287
Size: 703 584
BseYI C'CCAG_C
Cuts at: 0 149 1287
Size: 149 1138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 1287
Size: 137 1150
BsiEI CG_ry'CG
Cuts at: 0 131 1287
Size: 131 1156
BsiHKAI G_wGCw'C
Cuts at: 0 685 1287
Size: 685 602
BsmI GAATG_Cn'
Cuts at: 0 1173 1287
Size: 1173 114
Bsp1286I G_dGCh'C
Cuts at: 0 685 1287
Size: 685 602
BspHI T'CATG_A
Cuts at: 0 311 1287
Size: 311 976
BsrBI CCG'CTC
Cuts at: 0 1230 1287
Size: 1230 57
BssHII G'CGCG_C
Cuts at: 0 36 1287
Size: 36 1251
BstXI CCAn_nnnn'nTGG
Cuts at: 0 104 1287
Size: 104 1183
BstYI r'GATC_y
Cuts at: 0 403 1287
Size: 403 884
Bsu36I CC'TnA_GG
Cuts at: 0 848 1287
Size: 848 439
BtgI C'CryG_G
Cuts at: 0 41 97 1287
Size: 41 56 1190
Fragments arranged by size:
1190 56 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 333 1287
Size: 333 954
BtsI GCAGTG_nn'
Cuts at: 0 808 1287
Size: 808 479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1077 1112 1287
Size: 1077 35 175
Fragments arranged by size:
1077 175 35
EaeI y'GGCC_r
Cuts at: 0 128 1160 1287
Size: 128 1032 127
Fragments arranged by size:
1032 128 127
EagI C'GGCC_G
Cuts at: 0 128 1287
Size: 128 1159
EarI CTCTTCn'nnn_
Cuts at: 0 184 298 536 766 1110 1287
Size: 184 114 238 230 344 177
Fragments arranged by size:
344 238 230 184 177 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 416 1287
Size: 416 871
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 910 1287
Size: 102 808 377
Fragments arranged by size:
808 377 102
EcoO109I rG'GnC_Cy
Cuts at: 0 250 952 1155 1287
Size: 250 702 203 132
Fragments arranged by size:
702 250 203 132
HaeII r_GCGC'y
Cuts at: 0 863 1287
Size: 863 424
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 75 107 395 427 451 483 1246 1278
Size: 75 32 288 32 24 32 763 32
Cuts at: 1278 1287
Size: 9
Fragments arranged by size:
763 288 75 32 32 32 32 24
9
HincII GTy'rAC
Cuts at: 0 828 1287
Size: 828 459
MfeI C'AATT_G
Cuts at: 0 536 1287
Size: 536 751
MslI CAynn'nnrTG
Cuts at: 0 102 1287
Size: 102 1185
MspA1I CmG'CkG
Cuts at: 0 43 66 80 121 127 602 1212 1284
Size: 43 23 14 41 6 475 610 72
Cuts at: 1284 1287
Size: 3
Fragments arranged by size:
610 475 72 43 41 23 14 6
3
NcoI C'CATG_G
Cuts at: 0 97 1287
Size: 97 1190
NspI r_CATG'y
Cuts at: 0 157 1287
Size: 157 1130
PasI CC'CwG_GG
Cuts at: 0 159 1287
Size: 159 1128
PflMI CCAn_nnn'nTGG
Cuts at: 0 80 1287
Size: 80 1207
PpuMI rG'GwC_Cy
Cuts at: 0 952 1287
Size: 952 335
PstI C_TGCA'G
Cuts at: 0 126 1252 1287
Size: 126 1126 35
Fragments arranged by size:
1126 126 35
PvuII CAG'CTG
Cuts at: 0 80 121 602 1212 1284 1287
Size: 80 41 481 610 72 3
Fragments arranged by size:
610 481 80 72 41 3
SacII CC_GC'GG
Cuts at: 0 44 1287
Size: 44 1243
SapI GCTCTTCn'nnn_
Cuts at: 0 1110 1287
Size: 1110 177
ScaI AGT'ACT
Cuts at: 0 895 1072 1287
Size: 895 177 215
Fragments arranged by size:
895 215 177
SfcI C'TryA_G
Cuts at: 0 122 1248 1287
Size: 122 1126 39
Fragments arranged by size:
1126 122 39
SmlI C'TyrA_G
Cuts at: 0 173 766 1076 1287
Size: 173 593 310 211
Fragments arranged by size:
593 310 211 173
SphI G_CATG'C
Cuts at: 0 157 1287
Size: 157 1130
SspI AAT'ATT
Cuts at: 0 230 1287
Size: 230 1057
StyI C'CwwG_G
Cuts at: 0 97 926 1088 1287
Size: 97 829 162 199
Fragments arranged by size:
829 199 162 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 317 1287
Size: 317 970
TatI w'GTAC_w
Cuts at: 0 596 893 1070 1287
Size: 596 297 177 217
Fragments arranged by size:
596 297 217 177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 99 131 573 605 695 727 1287
Size: 99 32 442 32 90 32 560
Fragments arranged by size:
560 442 99 90 32 32 32
Enzymes that do cut:
AlwNI AseI BamHI BanI BciVI BpmI Bpu10I BpuEI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BsrBI BssHII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI EaeI EagI EarI EciI
Eco57MI EcoO109I HaeII Hin4I HincII MfeI MslI MspA1I
NcoI NspI PasI PflMI PpuMI PstI PvuII SacII
SapI ScaI SfcI SmlI SphI SspI StyI TaqII
TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
ApoI AscI AsiSI AvaI AvrII BaeI BanII BbeI
BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BsaAI BsaBI BsaWI
BsiWI BsmBI BspEI BspMI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstZ17I ClaI DraI DraIII DrdI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MluI MmeI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI StuI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI