Restriction map for
pFN21AE2701
(Linear) (Six-base) MAPSORT of: KIEE2701.seq Check: 4438 from: 1 to: 13641
KIEE2701.orf
With 182 enzymes: SgfI *
August 18, 2017 10:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2439 4494 5754 6318 6537 6813 7845 10656
Size: 2439 2055 1260 564 219 276 1032 2811
Cuts at: 10656 13315 13641
Size: 2659 326
Fragments arranged by size:
2811 2659 2439 2055 1260 1032 564 326
276 219
AatII G_ACGT'C
Cuts at: 0 8711 12391 13641
Size: 8711 3680 1250
Fragments arranged by size:
8711 3680 1250
AccI GT'mk_AC
Cuts at: 0 8684 11084 13641
Size: 8684 2400 2557
Fragments arranged by size:
8684 2557 2400
Acc65I G'GTAC_C
Cuts at: 0 521 13641
Size: 521 13120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 985 1165 1495 2020 2173 2401 2770
Size: 859 126 180 330 525 153 228 369
Cuts at: 2770 3415 5809 6370 6655 8128 9151 9379 9897
Size: 645 2394 561 285 1473 1023 228 518
Cuts at: 9897 10464 11968 12124 12382 13141 13641
Size: 567 1504 156 258 759 500
Fragments arranged by size:
2394 1504 1473 1023 859 759 645 567
561 525 518 500 369 330 285 258
228 228 180 156 153 126
AfeI AGC'GCT
Cuts at: 0 2950 3526 3559 4492 4780 4897 6790 7137
Size: 2950 576 33 933 288 117 1893 347
Cuts at: 7137 7213 7519 8608 9013 12985 13156 13641
Size: 76 306 1089 405 3972 171 485
Fragments arranged by size:
3972 2950 1893 1089 933 576 485 405
347 306 288 171 117 76 33
AflIII A'CryG_T
Cuts at: 0 4797 13641
Size: 4797 8844
AgeI A'CCGG_T
Cuts at: 0 11917 12976 13641
Size: 11917 1059 665
Fragments arranged by size:
11917 1059 665
AhdI GACnn_n'nnGTC
Cuts at: 0 744 11508 13641
Size: 744 10764 2133
Fragments arranged by size:
10764 2133 744
AleI CACnn'nnGTG
Cuts at: 0 188 2471 3051 8032 8884 10666 13641
Size: 188 2283 580 4981 852 1782 2975
Fragments arranged by size:
4981 2975 2283 1782 852 580 188
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4357 4733 7877 8428 8956 10405 12139 13396
Size: 4357 376 3144 551 528 1449 1734 1257
Cuts at: 13396 13641
Size: 245
Fragments arranged by size:
4357 3144 1734 1449 1257 551 528 376
245
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 901 902 933 934 13641
Size: 901 1 31 1 12707
Fragments arranged by size:
12707 901 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 0 531 963 1581 2220 2634 2901 2904 2913
Size: 531 432 618 639 414 267 3 9
Cuts at: 2913 3759 4272 4431 4611 5124 5193 5256 5364
Size: 846 513 159 180 513 69 63 108
Cuts at: 5364 6204 6531 6561 6633 7068 7299 7452 7470
Size: 840 327 30 72 435 231 153 18
Cuts at: 7470 7587 7641 7725 7779 7800 7971 8160 8601
Size: 117 54 84 54 21 171 189 441
Cuts at: 8601 8997 9111 9357 9390 9807 10064 10218 10278
Size: 396 114 246 33 417 257 154 60
Cuts at: 10278 10578 10908 11109 12930 13119 13176 13272 13641
Size: 300 330 201 1821 189 57 96 369
Fragments arranged by size:
1821 846 840 639 618 531 513 513
441 435 432 417 414 396 369 330
327 300 267 257 246 231 201 189
189 180 171 159 154 153 117 114
108 96 84 72 69 63 60 57
54 54 33 30 21 18 9 3
ApaI G_GGCC'C
Cuts at: 0 2497 3321 3411 4602 8281 9265 9510 9657
Size: 2497 824 90 1191 3679 984 245 147
Cuts at: 9657 10258 10806 11264 11992 12835 13624 13641
Size: 601 548 458 728 843 789 17
Fragments arranged by size:
3679 2497 1191 984 843 824 789 728
601 548 458 245 147 90 17
ApaLI G'TGCA_C
Cuts at: 0 2464 2924 3010 4436 8455 10432 13641
Size: 2464 460 86 1426 4019 1977 3209
Fragments arranged by size:
4019 3209 2464 1977 1426 460 86
ApoI r'AATT_y
Cuts at: 0 1699 6514 13641
Size: 1699 4815 7127
Fragments arranged by size:
7127 4815 1699
AvaI C'yCGr_G
Cuts at: 0 31 249 266 1543 1757 2842 3373 3604
Size: 31 218 17 1277 214 1085 531 231
Cuts at: 3604 6287 6380 8281 8623 8702 9265 9377 9476
Size: 2683 93 1901 342 79 563 112 99
Cuts at: 9476 9650 10258 10320 10600 10789 10978 11029 11263
Size: 174 608 62 280 189 189 51 234
Cuts at: 11263 11992 12436 12708 13490 13572 13596 13641
Size: 729 444 272 782 82 24 45
Fragments arranged by size:
2683 1901 1277 1085 782 729 608 563
531 444 342 280 272 234 231 218
214 189 189 174 112 99 93 82
79 62 51 45 31 24 17
BamHI G'GATC_C
Cuts at: 0 12751 13641
Size: 12751 890
BanI G'GyrC_C
Cuts at: 0 332 521 1068 1388 1538 1762 2571 2648
Size: 332 189 547 320 150 224 809 77
Cuts at: 2648 3658 4649 5107 5945 6065 8940 9064 9187
Size: 1010 991 458 838 120 2875 124 123
Cuts at: 9187 9516 9593 11005 11588 11605 11791 12599 13285
Size: 329 77 1412 583 17 186 808 686
Cuts at: 13285 13641
Size: 356
Fragments arranged by size:
2875 1412 1010 991 838 809 808 686
583 547 458 356 332 329 320 224
189 186 150 124 123 120 77 77
17
BanII G_rGCy'C
Cuts at: 0 2024 2497 2966 3321 3374 3411 3605 4285
Size: 2024 473 469 355 53 37 194 680
Cuts at: 4285 4602 6387 6689 7185 7265 8045 8281 8987
Size: 317 1785 302 496 80 780 236 706
Cuts at: 8987 9057 9265 9510 9584 9657 9671 9767 9950
Size: 70 208 245 74 73 14 96 183
Cuts at: 9950 9978 10036 10258 10806 11071 11264 11519 11897
Size: 28 58 222 548 265 193 255 378
Cuts at: 11897 11992 12835 13199 13624 13641
Size: 95 843 364 425 17
Fragments arranged by size:
2024 1785 843 780 706 680 548 496
473 469 425 378 364 355 317 302
265 255 245 236 222 208 194 193
183 96 95 80 74 73 70 58
53 37 28 17 14
BbeI G_GCGC'C
Cuts at: 0 1542 3662 4653 5949 6069 12603 13289 13641
Size: 1542 2120 991 1296 120 6534 686 352
Fragments arranged by size:
6534 2120 1542 1296 991 686 352 120
BbsI GAAGACnn'nnnn_
Cuts at: 0 209 1171 4768 5471 8063 10888 11805 12391
Size: 209 962 3597 703 2592 2825 917 586
Cuts at: 12391 13641
Size: 1250
Fragments arranged by size:
3597 2825 2592 1250 962 917 703 586
209
BbvCI CC'TCA_GC
Cuts at: 0 731 5321 5749 5957 6269 6980 7139 7196
Size: 731 4590 428 208 312 711 159 57
Cuts at: 7196 7763 9967 10646 10946 11563 13641
Size: 567 2204 679 300 617 2078
Fragments arranged by size:
4590 2204 2078 731 711 679 617 567
428 312 300 208 159 57
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2677 2711 3099 3133 4158 4192 7245 7279
Size: 2677 34 388 34 1025 34 3053 34
Cuts at: 7279 8073 8107 8955 8989 13641
Size: 794 34 848 34 4652
Fragments arranged by size:
4652 3053 2677 1025 848 794 388 34
34 34 34 34 34
BclI T'GATC_A
Cuts at: 0 10853 12578 13641
Size: 10853 1725 1063
Fragments arranged by size:
10853 1725 1063
BglI GCCn_nnn'nGGC
Cuts at: 0 997 1676 2359 2599 3355 3385 3895 4315
Size: 997 679 683 240 756 30 510 420
Cuts at: 4315 4403 4579 4606 4864 4918 5269 5311 5404
Size: 88 176 27 258 54 351 42 93
Cuts at: 5404 6121 6262 6652 6865 7825 9260 9298 9514
Size: 717 141 390 213 960 1435 38 216
Cuts at: 9514 10073 10150 10240 10291 10742 11155 11410 13060
Size: 559 77 90 51 451 413 255 1650
Cuts at: 13060 13114 13641
Size: 54 527
Fragments arranged by size:
1650 1435 997 960 756 717 683 679
559 527 510 451 420 413 390 351
258 255 240 216 213 176 141 93
90 88 77 54 54 51 42 38
30 27
BglII A'GATC_T
Cuts at: 0 434 8354 13328 13641
Size: 434 7920 4974 313
Fragments arranged by size:
7920 4974 434 313
BlpI GC'TnA_GC
Cuts at: 0 2524 2861 4052 4328 4829 6718 7145 7358
Size: 2524 337 1191 276 501 1889 427 213
Cuts at: 7358 9619 11291 13641
Size: 2261 1672 2350
Fragments arranged by size:
2524 2350 2261 1889 1672 1191 501 427
337 276 213
Bme1580I G_kGCm'C
Cuts at: 0 1073 1725 2468 2497 2555 2576 2928 3014
Size: 1073 652 743 29 58 21 352 86
Cuts at: 3014 3060 3321 3411 3468 4440 4602 4842 5424
Size: 46 261 90 57 972 162 240 582
Cuts at: 5424 6450 8281 8459 8474 9197 9265 9458 9510
Size: 1026 1831 178 15 723 68 193 52
Cuts at: 9510 9657 9930 10258 10436 10806 11008 11264 11992
Size: 147 273 328 178 370 202 256 728
Cuts at: 11992 12835 13220 13375 13624 13641
Size: 843 385 155 249 17
Fragments arranged by size:
1831 1073 1026 972 843 743 728 723
652 582 385 370 352 328 273 261
256 249 240 202 193 178 178 162
155 147 90 86 68 58 57 52
46 29 21 17 15
BmgBI CAC'GTC
Cuts at: 0 720 801 1365 2706 3513 3588 8061 8481
Size: 720 81 564 1341 807 75 4473 420
Cuts at: 8481 10458 13281 13641
Size: 1977 2823 360
Fragments arranged by size:
4473 2823 1977 1341 807 720 564 420
360 81 75
BmrI ACTGGGnnnn_n'
Cuts at: 0 3622 9972 12044 13641
Size: 3622 6350 2072 1597
Fragments arranged by size:
6350 3622 2072 1597
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 264 1989 2892 4569 5352 6708 9690 10050
Size: 264 1725 903 1677 783 1356 2982 360
Cuts at: 10050 13641
Size: 3591
Fragments arranged by size:
3591 2982 1725 1677 1356 903 783 360
264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 537 579 667 909 1057 1153 1192
Size: 259 278 42 88 242 148 96 39
Cuts at: 1192 1570 1864 1950 2014 2299 2635 2806 3007
Size: 378 294 86 64 285 336 171 201
Cuts at: 3007 3220 3241 3274 3784 3835 4126 4633 4720
Size: 213 21 33 510 51 291 507 87
Cuts at: 4720 4795 4912 5101 5215 5386 5659 5737 5851
Size: 75 117 189 114 171 273 78 114
Cuts at: 5851 5857 5920 6043 6604 7123 7147 7321 7384
Size: 6 63 123 561 519 24 174 63
Cuts at: 7384 7567 7747 7822 8209 8437 8623 8980 9112
Size: 183 180 75 387 228 186 357 132
Cuts at: 9112 9568 10105 10186 10414 10600 11191 11479 11920
Size: 456 537 81 228 186 591 288 441
Cuts at: 11920 12148 12526 12771 12886 12955 13183 13411 13641
Size: 228 378 245 115 69 228 228 230
Fragments arranged by size:
591 561 537 519 510 507 456 441
387 378 378 357 336 294 291 288
285 278 273 259 245 242 230 228
228 228 228 228 213 201 189 186
186 183 180 174 171 171 148 132
123 117 115 114 114 96 88 87
86 81 78 75 75 69 64 63
63 51 42 39 33 24 21 6
Bpu10I CC'TnA_GC
Cuts at: 0 88 731 3313 5321 5749 5957 6269 6386
Size: 88 643 2582 2008 428 208 312 117
Cuts at: 6386 6980 7139 7196 7763 9967 10646 10946 11563
Size: 594 159 57 567 2204 679 300 617
Cuts at: 11563 11581 13641
Size: 18 2060
Fragments arranged by size:
2582 2204 2060 2008 679 643 617 594
567 428 312 300 208 159 117 88
57 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1048 1360 3247 3319 3574 3898 4891 5527
Size: 1048 312 1887 72 255 324 993 636
Cuts at: 5527 5551 6712 6919 6958 7138 7396 9976 11113
Size: 24 1161 207 39 180 258 2580 1137
Cuts at: 11113 13309 13641
Size: 2196 332
Fragments arranged by size:
2580 2196 1887 1161 1137 1048 993 636
332 324 312 258 255 207 180 72
39 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 959 6583 9490 9688 9880 10304 10651 13641
Size: 959 5624 2907 198 192 424 347 2990
Fragments arranged by size:
5624 2990 2907 959 424 347 198 192
BsaAI yAC'GTr
Cuts at: 0 1122 2661 3948 4800 8588 10565 10926 11538
Size: 1122 1539 1287 852 3788 1977 361 612
Cuts at: 11538 13641
Size: 2103
Fragments arranged by size:
3788 2103 1977 1539 1287 1122 852 612
361
BsaBI GATnn'nnATC
Cuts at: 0 943 8875 10858 12544 13641
Size: 943 7932 1983 1686 1097
Fragments arranged by size:
7932 1983 1686 1097 943
BsaHI Gr'CG_yC
Cuts at: 0 779 1247 1539 3440 3659 3674 4650 5946
Size: 779 468 292 1901 219 15 976 1296
Cuts at: 5946 6066 7205 8708 9404 9530 11636 11822 12388
Size: 120 1139 1503 696 126 2106 186 566
Cuts at: 12388 12600 13001 13253 13286 13520 13641
Size: 212 401 252 33 234 121
Fragments arranged by size:
2106 1901 1503 1296 1139 976 779 696
566 468 401 292 252 234 219 212
186 126 121 120 33 15
BsaWI w'CCGG_w
Cuts at: 0 7217 11917 12896 12976 13641
Size: 7217 4700 979 80 665
Fragments arranged by size:
7217 4700 979 665 80
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 214 244 270 300 535 565 1090 1120
Size: 214 30 26 30 235 30 525 30
Cuts at: 1120 1873 1903 5125 5155 6076 6106 8677 8707
Size: 753 30 3222 30 921 30 2571 30
Cuts at: 8707 9892 9922 10090 10120 10729 10759 11004 11034
Size: 1185 30 168 30 609 30 245 30
Cuts at: 11034 12514 12544 12577 12607 12859 12889 13156 13186
Size: 1480 30 33 30 252 30 267 30
Cuts at: 13186 13641
Size: 455
Fragments arranged by size:
3222 2571 1480 1185 921 753 609 525
455 267 252 245 235 214 168 33
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30 30
26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 949 1240 1612 1809 1921 1924 1927 2773
Size: 949 291 372 197 112 3 3 846
Cuts at: 2773 3319 3340 3781 3811 4048 4051 4054 4084
Size: 546 21 441 30 237 3 3 30
Cuts at: 4084 4210 4228 4273 4366 4369 4462 4495 4630
Size: 126 18 45 93 3 93 33 135
Cuts at: 4630 4651 4837 4888 4960 5014 5053 5212 5215
Size: 21 186 51 72 54 39 159 3
Cuts at: 5215 5338 5341 5416 5656 5689 5785 5788 5947
Size: 123 3 75 240 33 96 3 159
Cuts at: 5947 5950 5974 6004 6007 6070 6286 6295 6379
Size: 3 24 30 3 63 216 9 84
Cuts at: 6379 6601 6634 6694 6697 6727 6817 6847 6907
Size: 222 33 60 3 30 90 30 60
Cuts at: 6907 7090 7213 7246 7435 7609 7672 7702 7726
Size: 183 123 33 189 174 63 30 24
Cuts at: 7726 7765 7780 7819 7852 8014 8524 8905 9109
Size: 39 15 39 33 162 510 381 204
Cuts at: 9109 9508 9679 9892 10501 10693 10696 10807 11395
Size: 399 171 213 609 192 3 111 588
Cuts at: 11395 11698 11770 11977 12181 12235 12301 12540 12543
Size: 303 72 207 204 54 66 239 3
Cuts at: 12543 12550 12732 12747 12750 12838 13381 13557 13560
Size: 7 182 15 3 88 543 176 3
Cuts at: 13560 13641
Size: 81
Fragments arranged by size:
949 846 609 588 546 543 510 441
399 381 372 303 291 240 239 237
222 216 213 207 204 204 197 192
189 186 183 182 176 174 171 162
159 159 135 126 123 123 112 111
96 93 93 90 88 84 81 75
72 72 66 63 63 60 60 54
54 51 45 39 39 39 33 33
33 33 33 30 30 30 30 30
30 24 24 21 21 18 15 15
9 7 3 3 3 3 3 3
3 3 3 3 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 0 1645 1662 2032 2394 2621 3059 3105 3210
Size: 1645 17 370 362 227 438 46 105
Cuts at: 3210 3545 5687 5841 7208 7580 8536 9407 9436
Size: 335 2142 154 1367 372 956 871 29
Cuts at: 9436 9550 10029 10513 10805 11405 11518 12794 12970
Size: 114 479 484 292 600 113 1276 176
Cuts at: 12970 13641
Size: 671
Fragments arranged by size:
2142 1645 1367 1276 956 871 671 600
484 479 438 372 370 362 335 292
227 176 154 114 113 105 46 29
17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 409 823 1102 1387 1414 1552 1666
Size: 148 261 414 279 285 27 138 114
Cuts at: 1666 2395 2483 2728 2761 2776 3665 4528 4739
Size: 729 88 245 33 15 889 863 211
Cuts at: 4739 5989 6193 6313 6724 6976 7234 7312 7456
Size: 1250 204 120 411 252 258 78 144
Cuts at: 7456 8272 11254 12933 13189 13641
Size: 816 2982 1679 256 452
Fragments arranged by size:
2982 1679 1250 889 863 816 729 452
414 411 285 279 261 258 256 252
245 211 204 148 144 138 120 114
88 78 33 27 15
BsiEI CG_ry'CG
Cuts at: 0 570 899 1938 5382 5813 5987 8309 8979
Size: 570 329 1039 3444 431 174 2322 670
Cuts at: 8979 10833 11277 12020 12588 12645 13109 13584 13641
Size: 1854 444 743 568 57 464 475 57
Fragments arranged by size:
3444 2322 1854 1039 743 670 570 568
475 464 444 431 329 174 57 57
BsiHKAI G_wGCw'C
Cuts at: 0 89 2024 2468 2525 2928 2966 3014 3314
Size: 89 1935 444 57 403 38 48 300
Cuts at: 3314 3374 3605 4285 4440 6689 7265 7931 8045
Size: 60 231 680 155 2249 576 666 114
Cuts at: 8045 8402 8459 8987 9584 9671 9767 9950 10436
Size: 357 57 528 597 87 96 183 486
Cuts at: 10436 11897 13641
Size: 1461 1744
Fragments arranged by size:
2249 1935 1744 1461 680 666 597 576
528 486 444 403 357 300 231 183
155 114 96 89 87 60 57 57
48 38
BsiWI C'GTAC_G
Cuts at: 0 3958 13641
Size: 3958 9683
BsmI GAATG_Cn'
Cuts at: 0 4250 13641
Size: 4250 9391
BsmBI CGTCTCn'nnnn_
Cuts at: 0 557 768 2992 4696 5155 7072 7453 8620
Size: 557 211 2224 1704 459 1917 381 1167
Cuts at: 8620 9017 9345 10597 12433 12789 12820 12853 13641
Size: 397 328 1252 1836 356 31 33 788
Fragments arranged by size:
2224 1917 1836 1704 1252 1167 788 557
459 397 381 356 328 211 33 31
Bsp1286I G_dGCh'C
Cuts at: 0 89 1073 1725 2024 2468 2497 2525 2555
Size: 89 984 652 299 444 29 28 30
Cuts at: 2555 2576 2928 2966 3014 3060 3314 3321 3374
Size: 21 352 38 48 46 254 7 53
Cuts at: 3374 3411 3468 3605 4285 4440 4602 4842 5424
Size: 37 57 137 680 155 162 240 582
Cuts at: 5424 6387 6450 6689 7185 7265 7931 8045 8281
Size: 963 63 239 496 80 666 114 236
Cuts at: 8281 8402 8459 8474 8987 9057 9197 9265 9458
Size: 121 57 15 513 70 140 68 193
Cuts at: 9458 9510 9584 9657 9671 9767 9930 9950 9978
Size: 52 74 73 14 96 163 20 28
Cuts at: 9978 10036 10258 10436 10806 11008 11071 11264 11519
Size: 58 222 178 370 202 63 193 255
Cuts at: 11519 11897 11992 12835 13199 13220 13375 13624 13641
Size: 378 95 843 364 21 155 249 17
Fragments arranged by size:
984 963 843 680 666 652 582 513
496 444 378 370 364 352 299 255
254 249 240 239 236 222 202 193
193 178 163 162 155 155 140 137
121 114 96 95 89 80 74 73
70 68 63 63 58 57 57 53
52 48 46 38 37 30 29 28
28 21 21 20 17 15 14 7
BspEI T'CCGG_A
Cuts at: 0 7217 13641
Size: 7217 6424
BspHI T'CATG_A
Cuts at: 0 9273 13641
Size: 9273 4368
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 905 1580 1589 1649 1802 2294 2439 3216
Size: 905 675 9 60 153 492 145 777
Cuts at: 3216 3962 4494 4674 5754 6318 6537 6617 6813
Size: 746 532 180 1080 564 219 80 196
Cuts at: 6813 7845 8081 8600 8828 9149 10656 11000 11096
Size: 1032 236 519 228 321 1507 344 96
Cuts at: 11096 11691 12311 13315 13641
Size: 595 620 1004 326
Fragments arranged by size:
1507 1080 1032 1004 905 777 746 675
620 595 564 532 519 492 344 326
321 236 228 219 196 180 153 145
96 80 60 9
BsrBI CCG'CTC
Cuts at: 0 117 681 1045 1137 1719 2720 3507 3537
Size: 117 564 364 92 582 1001 787 30
Cuts at: 3537 4080 4161 4647 4833 4863 4884 5496 5721
Size: 543 81 486 186 30 21 612 225
Cuts at: 5721 5940 6036 6082 6345 7113 9633 10584 11220
Size: 219 96 46 263 768 2520 951 636
Cuts at: 11220 11295 12381 12593 12647 12731 13641
Size: 75 1086 212 54 84 910
Fragments arranged by size:
2520 1086 1001 951 910 787 768 636
612 582 564 543 486 364 263 225
219 212 186 117 96 92 84 81
75 54 46 30 30 21
BsrFI r'CCGG_y
Cuts at: 0 593 632 2039 2849 3042 3354 3882 4337
Size: 593 39 1407 810 193 312 528 455
Cuts at: 4337 5377 5381 7981 8234 8489 8909 9184 9564
Size: 1040 4 2600 253 255 420 275 380
Cuts at: 9564 9596 10072 10300 10828 11305 11416 11608 11917
Size: 32 476 228 528 477 111 192 309
Cuts at: 11917 12176 12670 12718 12976 13147 13180 13471 13483
Size: 259 494 48 258 171 33 291 12
Cuts at: 13483 13495 13525 13641
Size: 12 30 116
Fragments arranged by size:
2600 1407 1040 810 593 528 528 494
477 476 455 420 380 312 309 291
275 259 258 255 253 228 193 192
171 116 111 48 39 33 32 30
12 12 4
BsrGI T'GTAC_A
Cuts at: 0 10670 13641
Size: 10670 2971
BssHII G'CGCG_C
Cuts at: 0 4582 4867 8313 12024 13641
Size: 4582 285 3446 3711 1617
Fragments arranged by size:
4582 3711 3446 1617 285
BssSI C'ACGA_G
Cuts at: 0 1432 3322 8575 10228 10267 11254 13641
Size: 1432 1890 5253 1653 39 987 2387
Fragments arranged by size:
5253 2387 1890 1653 1432 987 39
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1530 1581 2220 2655 2904 3018 3570 3759
Size: 1530 51 639 435 249 114 552 189
Cuts at: 3759 4188 4272 4431 4435 6204 6222 6531 6954
Size: 429 84 159 4 1769 18 309 423
Cuts at: 6954 7434 7452 7470 9247 9390 10064 11798 12930
Size: 480 18 18 1777 143 674 1734 1132
Cuts at: 12930 13641
Size: 711
Fragments arranged by size:
1777 1769 1734 1530 1132 711 674 639
552 480 435 429 423 309 249 189
159 143 114 84 51 18 18 18
4
BstEII G'GTnAC_C
Cuts at: 0 7281 8763 9834 10836 11301 11760 13641
Size: 7281 1482 1071 1002 465 459 1881
Fragments arranged by size:
7281 1881 1482 1071 1002 465 459
BstXI CCAn_nnnn'nTGG
Cuts at: 0 454 2032 3252 3276 3711 13641
Size: 454 1578 1220 24 435 9930
Fragments arranged by size:
9930 1578 1220 454 435 24
BstYI r'GATC_y
Cuts at: 0 434 944 3695 4295 4358 5777 7304 8354
Size: 434 510 2751 600 63 1419 1527 1050
Cuts at: 8354 12233 12751 13328 13641
Size: 3879 518 577 313
Fragments arranged by size:
3879 2751 1527 1419 1050 600 577 518
510 434 313 63
Bsu36I CC'TnA_GG
Cuts at: 0 713 1834 8205 12164 13641
Size: 713 1121 6371 3959 1477
Fragments arranged by size:
6371 3959 1477 1121 713
BtgI C'CryG_G
Cuts at: 0 2072 3285 3780 5129 6647 7907 8030 8432
Size: 2072 1213 495 1349 1518 1260 123 402
Cuts at: 8432 8474 9161 9230 9416 9725 9839 10409 10737
Size: 42 687 69 186 309 114 570 328
Cuts at: 10737 11072 11655 11678 11717 12111 12143 12185 12836
Size: 335 583 23 39 394 32 42 651
Cuts at: 12836 13506 13634 13641
Size: 670 128 7
Fragments arranged by size:
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583 570 495 402 394 335 328 309
186 128 123 114 69 42 42 39
32 23 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 227 357 549 3051 3152 7338 9060 11787
Size: 227 130 192 2502 101 4186 1722 2727
Cuts at: 11787 12609 13641
Size: 822 1032
Fragments arranged by size:
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130 101
BtsI GCAGTG_nn'
Cuts at: 0 2113 2230 4429 4722 6568 7844 9248 9785
Size: 2113 117 2199 293 1846 1276 1404 537
Cuts at: 9785 10065 13641
Size: 280 3576
Fragments arranged by size:
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ClaI AT'CG_AT
Cuts at: 0 12614 13641
Size: 12614 1027
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1487 1522 12702 12737 13641
Size: 1487 35 11180 35 904
Fragments arranged by size:
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DraIII CAC_nnn'GTG
Cuts at: 0 149 1614 1729 2645 4435 8469 9453 10446
Size: 149 1465 115 916 1790 4034 984 993
Cuts at: 10446 13641
Size: 3195
Fragments arranged by size:
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115
DrdI GACnn_nn'nnGTC
Cuts at: 0 724 12565 13101 13641
Size: 724 11841 536 540
Fragments arranged by size:
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EaeI y'GGCC_r
Cuts at: 0 216 476 567 896 1124 2351 2429 2732
Size: 216 260 91 329 228 1227 78 303
Cuts at: 2732 3356 3713 3896 4085 4124 5126 5270 5303
Size: 624 357 183 189 39 1002 144 33
Cuts at: 5303 5379 5396 5456 5516 5810 5984 7166 7571
Size: 76 17 60 60 294 174 1182 405
Cuts at: 7571 7646 7884 8306 8346 9158 9566 10178 10830
Size: 75 238 422 40 812 408 612 652
Cuts at: 10830 11411 11610 11912 12017 13106 13149 13581 13641
Size: 581 199 302 105 1089 43 432 60
Fragments arranged by size:
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302 294 260 238 228 216 199 189
183 174 144 105 91 78 76 75
60 60 60 43 40 39 33 17
EagI C'GGCC_G
Cuts at: 0 567 896 5379 5810 5984 8306 10830 12017
Size: 567 329 4483 431 174 2322 2524 1187
Cuts at: 12017 13106 13581 13641
Size: 1089 475 60
Fragments arranged by size:
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EarI CTCTTCn'nnn_
Cuts at: 0 580 1360 2665 2927 4957 5426 5968 6143
Size: 580 780 1305 262 2030 469 542 175
Cuts at: 6143 6329 6694 7561 8368 10345 10697 11350 12079
Size: 186 365 867 807 1977 352 653 729
Cuts at: 12079 12217 13557 13641
Size: 138 1340 84
Fragments arranged by size:
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653 580 542 469 365 352 262 186
175 138 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 691 1348 2250 4171 4306 4459 4468 4552
Size: 691 657 902 1921 135 153 9 84
Cuts at: 4552 4648 4657 4741 4957 5020 5050 5116 5443
Size: 96 9 84 216 63 30 66 327
Cuts at: 5443 5506 5569 5596 5623 5839 5944 6160 6262
Size: 63 63 27 27 216 105 216 102
Cuts at: 6262 6292 6313 6469 6655 6844 7000 7087 7243
Size: 30 21 156 186 189 156 87 156
Cuts at: 7243 7965 8398 8545 10375 10522 10651 10885 11031
Size: 722 433 147 1830 147 129 234 146
Cuts at: 11031 11050 11863 12109 13641
Size: 19 813 246 1532
Fragments arranged by size:
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186 156 156 156 153 147 147 146
135 129 105 102 96 87 84 84
66 63 63 63 30 30 27 27
21 19 9 9
EcoICRI GAG'CTC
Cuts at: 0 2022 2964 3372 3603 4283 6687 7263 8043
Size: 2022 942 408 231 680 2404 576 780
Cuts at: 8043 8985 9582 9669 9765 9948 11895 13641
Size: 942 597 87 96 183 1947 1746
Fragments arranged by size:
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597 576 408 231 183 96 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 537 579 667 859 909 985 1057
Size: 259 278 42 88 192 50 76 72
Cuts at: 1057 1153 1165 1192 1495 1570 1864 1950 2014
Size: 96 12 27 303 75 294 86 64
Cuts at: 2014 2020 2173 2299 2401 2635 2770 2806 3007
Size: 6 153 126 102 234 135 36 201
Cuts at: 3007 3220 3241 3274 3415 3784 3835 4126 4633
Size: 213 21 33 141 369 51 291 507
Cuts at: 4633 4720 4795 4912 5101 5215 5386 5659 5737
Size: 87 75 117 189 114 171 273 78
Cuts at: 5737 5809 5851 5857 5920 6043 6370 6604 6655
Size: 72 42 6 63 123 327 234 51
Cuts at: 6655 7123 7147 7321 7384 7567 7747 7822 8128
Size: 468 24 174 63 183 180 75 306
Cuts at: 8128 8209 8437 8623 8980 9112 9151 9379 9568
Size: 81 228 186 357 132 39 228 189
Cuts at: 9568 9897 10105 10186 10414 10464 10600 11191 11479
Size: 329 208 81 228 50 136 591 288
Cuts at: 11479 11920 11968 12124 12148 12382 12526 12771 12886
Size: 441 48 156 24 234 144 245 115
Cuts at: 12886 12955 13141 13183 13411 13641
Size: 69 186 42 228 230
Fragments arranged by size:
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306 303 294 291 288 278 273 259
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114 102 96 88 87 86 81 81
78 76 75 75 75 72 72 69
64 63 63 51 51 50 50 48
42 42 42 39 36 33 27 24
24 21 12 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 169 238 658 2290 2917 3763 4657 4789
Size: 169 69 420 1632 627 846 894 132
Cuts at: 4789 4972 6799 7345 8188 8416 9349 9361 9490
Size: 183 1827 546 843 228 933 12 129
Cuts at: 9490 9895 10165 10342 10393 10762 10957 11020 11488
Size: 405 270 177 51 369 195 63 468
Cuts at: 11488 12076 12127 13123 13225 13641
Size: 588 51 996 102 416
Fragments arranged by size:
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228 195 183 177 169 132 129 102
69 63 51 51 12
EcoO109I rG'GnC_Cy
Cuts at: 0 36 92 243 528 684 1578 1838 2283
Size: 36 56 151 285 156 894 260 445
Cuts at: 2283 2494 2631 3317 3756 4599 4608 6897 7467
Size: 211 137 686 439 843 9 2289 570
Cuts at: 7467 8278 8334 8382 8619 8817 8925 9262 9318
Size: 811 56 48 237 198 108 337 56
Cuts at: 9318 9354 9366 9387 9506 9507 9720 9963 10017
Size: 36 12 21 119 1 213 243 54
Cuts at: 10017 10131 10255 10359 10596 10950 11250 11260 11385
Size: 114 124 104 237 354 300 10 125
Cuts at: 11385 11739 11989 12045 12093 12480 12791 12832 13014
Size: 354 250 56 48 387 311 41 182
Cuts at: 13014 13080 13116 13230 13601 13620 13621 13641
Size: 66 36 114 371 19 1 20
Fragments arranged by size:
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20 19 12 10 9 1 1
EcoRI G'AATT_C
Cuts at: 0 1699 13641
Size: 1699 11942
EcoRV GAT'ATC
Cuts at: 0 444 13641
Size: 444 13197
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 156 5814 8574 10551 11667 12285 13641
Size: 156 5658 2760 1977 1116 618 1356
Fragments arranged by size:
5658 2760 1977 1356 1116 618 156
FseI GG_CCGG'CC
Cuts at: 0 5381 10832 13151 13641
Size: 5381 5451 2319 490
Fragments arranged by size:
5451 5381 2319 490
FspI TGC'GCA
Cuts at: 0 4345 5695 5878 6415 7016 8932 11530 12409
Size: 4345 1350 183 537 601 1916 2598 879
Cuts at: 12409 13641
Size: 1232
Fragments arranged by size:
4345 2598 1916 1350 1232 879 601 537
183
HaeII r_GCGC'y
Cuts at: 0 1440 1542 1625 1981 2769 2952 3528 3561
Size: 1440 102 83 356 788 183 576 33
Cuts at: 3561 3662 3780 4398 4494 4653 4782 4899 4924
Size: 101 118 618 96 159 129 117 25
Cuts at: 4924 5124 5595 5682 5788 5949 6022 6069 6792
Size: 200 471 87 106 161 73 47 723
Cuts at: 6792 7139 7152 7215 7341 7521 8610 9015 9221
Size: 347 13 63 126 180 1089 405 206
Cuts at: 9221 10218 11562 11725 12417 12603 12987 13158 13289
Size: 997 1344 163 692 186 384 171 131
Cuts at: 13289 13641
Size: 352
Fragments arranged by size:
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576 471 405 384 356 352 347 206
200 186 183 180 171 163 161 159
131 129 126 118 117 106 102 101
96 87 83 73 63 47 33 25
13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 730 762 5314 5346 6076 6108 9892 9924
Size: 730 32 4552 32 730 32 3784 32
Cuts at: 9924 10090 10122 10303 10335 10959 10991 11023 11055
Size: 166 32 181 32 624 32 32 32
Cuts at: 11055 11494 11526 12037 12069 12410 12442 12577 12609
Size: 439 32 511 32 341 32 135 32
Cuts at: 12609 12691 12723 12859 12891 13641
Size: 82 32 136 32 750
Fragments arranged by size:
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341 181 166 136 135 82 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 156 8250 13008 13641
Size: 156 8094 4758 633
Fragments arranged by size:
8094 4758 633 156
HindIII A'AGCT_T
Cuts at: 0 7479 13641
Size: 7479 6162
KasI G'GCGC_C
Cuts at: 0 1538 3658 4649 5945 6065 12599 13285 13641
Size: 1538 2120 991 1296 120 6534 686 356
Fragments arranged by size:
6534 2120 1538 1296 991 686 356 120
KpnI G_GTAC'C
Cuts at: 0 525 13641
Size: 525 13116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 1241 4361 7410 9152 9176 9380 10757
Size: 443 798 3120 3049 1742 24 204 1377
Cuts at: 10757 11612 13641
Size: 855 2029
Fragments arranged by size:
3120 3049 2029 1742 1377 855 798 443
204 24
MscI TGG'CCA
Cuts at: 0 218 1126 2353 2734 5272 5305 7573 7648
Size: 218 908 1227 381 2538 33 2268 75
Cuts at: 7648 8348 11413 13641
Size: 700 3065 2228
Fragments arranged by size:
3065 2538 2268 2228 1227 908 700 381
218 75 33
MslI CAynn'nnrTG
Cuts at: 0 188 616 1948 2471 3051 8032 8881 8884
Size: 188 428 1332 523 580 4981 849 3
Cuts at: 8884 9160 10666 13641
Size: 276 1506 2975
Fragments arranged by size:
4981 2975 1506 1332 849 580 523 428
276 188 3
MspA1I CmG'CkG
Cuts at: 0 557 834 1089 1233 1299 1662 2127 2319
Size: 557 277 255 144 66 363 465 192
Cuts at: 2319 2379 2487 2901 2985 3030 3287 3495 3549
Size: 60 108 414 84 45 257 208 54
Cuts at: 3549 3624 3711 3741 3855 4062 4116 4122 4272
Size: 75 87 30 114 207 54 6 150
Cuts at: 4272 4596 4773 4815 5058 5091 5097 5131 5184
Size: 324 177 42 243 33 6 34 53
Cuts at: 5184 5190 5448 5454 5481 5628 5691 5757 5815
Size: 6 258 6 27 147 63 66 58
Cuts at: 5815 5916 5922 6009 6105 6111 6234 6487 6582
Size: 101 6 87 96 6 123 253 95
Cuts at: 6582 6630 6885 6912 7068 7095 7119 7125 7449
Size: 48 255 27 156 27 24 6 324
Cuts at: 7449 7512 7587 7605 7638 7725 7737 7791 7909
Size: 63 75 18 33 87 12 54 118
Cuts at: 7909 8010 8157 8811 8997 9411 9591 9609 9798
Size: 101 147 654 186 414 180 18 189
Cuts at: 9798 9917 10275 10575 10800 10809 11409 11508 11577
Size: 119 358 300 225 9 600 99 69
Cuts at: 11577 11616 11730 11798 12309 12360 12798 12927 13442
Size: 39 114 68 511 51 438 129 515
Cuts at: 13442 13459 13508 13641
Size: 17 49 133
Fragments arranged by size:
654 600 557 515 511 465 438 414
414 363 358 324 324 300 277 258
257 255 255 253 243 225 208 207
192 189 186 180 177 156 150 147
147 144 133 129 123 119 118 114
114 108 101 101 99 96 95 87
87 87 84 75 75 69 68 66
66 63 63 60 58 54 54 54
53 51 49 48 45 42 39 34
33 33 30 27 27 27 24 18
18 17 12 9 6 6 6 6
6 6 6
NaeI GCC'GGC
Cuts at: 0 3044 3356 4339 5379 7983 8911 9186 9566
Size: 3044 312 983 1040 2604 928 275 380
Cuts at: 9566 9598 10074 10830 12672 13149 13497 13641
Size: 32 476 756 1842 477 348 144
Fragments arranged by size:
3044 2604 1842 1040 983 928 756 477
476 380 348 312 275 144 32
NarI GG'CG_CC
Cuts at: 0 1539 3659 4650 5946 6066 12600 13286 13641
Size: 1539 2120 991 1296 120 6534 686 355
Fragments arranged by size:
6534 2120 1539 1296 991 686 355 120
NcoI C'CATG_G
Cuts at: 0 11678 12111 13641
Size: 11678 433 1530
Fragments arranged by size:
11678 1530 433
NgoMIV G'CCGG_C
Cuts at: 0 3042 3354 4337 5377 7981 8909 9184 9564
Size: 3042 312 983 1040 2604 928 275 380
Cuts at: 9564 9596 10072 10828 12670 13147 13495 13641
Size: 32 476 756 1842 477 348 146
Fragments arranged by size:
3042 2604 1842 1040 983 928 756 477
476 380 348 312 275 146 32
NotI GC'GGCC_GC
Cuts at: 0 5810 13641
Size: 5810 7831
NspI r_CATG'y
Cuts at: 0 4191 12705 13641
Size: 4191 8514 936
Fragments arranged by size:
8514 4191 936
PasI CC'CwG_GG
Cuts at: 0 13614 13641
Size: 13614 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 1035 1089 2490 2634 10812 13641
Size: 1035 54 1401 144 8178 2829
Fragments arranged by size:
8178 2829 1401 1035 144 54
PfoI T'CCnGG_A
Cuts at: 0 704 1130 3365 3938 7559 7799 8393 10997
Size: 704 426 2235 573 3621 240 594 2604
Cuts at: 10997 11783 11897 12787 12848 13160 13641
Size: 786 114 890 61 312 481
Fragments arranged by size:
3621 2604 2235 890 786 704 594 573
481 426 312 240 114 61
PmlI CAC'GTG
Cuts at: 0 1122 2661 4800 13641
Size: 1122 1539 2139 8841
Fragments arranged by size:
8841 2139 1539 1122
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 10939 10971 13641
Size: 10939 32 2670
Fragments arranged by size:
10939 2670 32
PpuMI rG'GwC_Cy
Cuts at: 0 243 684 1578 1838 2283 6897 8334 8619
Size: 243 441 894 260 445 4614 1437 285
Cuts at: 8619 8817 9318 9720 9963 10017 10596 10950 12045
Size: 198 501 402 243 54 579 354 1095
Cuts at: 12045 12791 13080 13601 13641
Size: 746 289 521 40
Fragments arranged by size:
4614 1437 1095 894 746 579 521 501
445 441 402 354 289 285 260 243
243 198 54 40
PshAI GACnn'nnGTC
Cuts at: 0 10009 13641
Size: 10009 3632
PspOMI G'GGCC_C
Cuts at: 0 2493 3317 3407 4598 8277 9261 9506 9653
Size: 2493 824 90 1191 3679 984 245 147
Cuts at: 9653 10254 10802 11260 11988 12831 13620 13641
Size: 601 548 458 728 843 789 21
Fragments arranged by size:
3679 2493 1191 984 843 824 789 728
601 548 458 245 147 90 21
PspXI vC'TCGA_Gb
Cuts at: 0 3373 3604 13641
Size: 3373 231 10037
Fragments arranged by size:
10037 3373 231
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 613 645 2152 2184 3988 4020 8572 8604
Size: 613 32 1507 32 1804 32 4552 32
Cuts at: 8604 10549 10581 12283 12315 13641
Size: 1945 32 1702 32 1326
Fragments arranged by size:
4552 1945 1804 1702 1507 1326 613 32
32 32 32 32 32
PstI C_TGCA'G
Cuts at: 0 302 833 1205 1259 1284 1526 1577 1799
Size: 302 531 372 54 25 242 51 222
Cuts at: 1799 2102 2225 2294 2884 2909 2984 3491 3671
Size: 303 123 69 590 25 75 507 180
Cuts at: 3671 3746 4181 4268 4379 4427 4607 4631 4736
Size: 75 435 87 111 48 180 24 105
Cuts at: 4736 4790 4940 5219 5235 6110 6143 6209 6405
Size: 54 150 279 16 875 33 66 196
Cuts at: 6405 6581 6629 6800 6998 7040 7409 7457 7475
Size: 176 48 171 198 42 369 48 18
Cuts at: 7475 7721 7796 7976 8009 8078 8150 8597 9002
Size: 246 75 180 33 69 72 447 405
Cuts at: 9002 9686 10055 11729 12308 13238 13641
Size: 684 369 1674 579 930 403
Fragments arranged by size:
1674 930 875 684 590 579 531 507
447 435 405 403 372 369 369 303
302 279 246 242 222 198 196 180
180 180 176 171 150 123 111 105
87 75 75 75 72 69 69 66
54 54 51 48 48 48 42 33
33 25 25 24 18 16
PvuII CAG'CTG
Cuts at: 0 557 834 1233 1662 2127 2319 2379 2487
Size: 557 277 399 429 465 192 60 108
Cuts at: 2487 2901 2985 3549 3624 3741 3855 4122 4272
Size: 414 84 564 75 117 114 267 150
Cuts at: 4272 4773 4815 5097 5190 5454 5691 5922 6105
Size: 501 42 282 93 264 237 231 183
Cuts at: 6105 6111 6234 6487 6582 6885 7068 7125 7449
Size: 6 123 253 95 303 183 57 324
Cuts at: 7449 7587 7725 7791 8157 8997 9411 9609 10275
Size: 138 138 66 366 840 414 198 666
Cuts at: 10275 10575 10809 11409 11508 11577 11616 11730 11798
Size: 300 234 600 99 69 39 114 68
Cuts at: 11798 12309 12798 13641
Size: 511 489 843
Fragments arranged by size:
843 840 666 600 564 557 511 501
489 465 429 414 414 399 366 324
303 300 282 277 267 264 253 237
234 231 198 192 183 183 150 138
138 123 117 114 114 108 99 95
93 84 75 69 68 66 60 57
42 39 6
SacI G_AGCT'C
Cuts at: 0 2024 2966 3374 3605 4285 6689 7265 8045
Size: 2024 942 408 231 680 2404 576 780
Cuts at: 8045 8987 9584 9671 9767 9950 11897 13641
Size: 942 597 87 96 183 1947 1744
Fragments arranged by size:
2404 2024 1947 1744 942 942 780 680
597 576 408 231 183 96 87
SacII CC_GC'GG
Cuts at: 0 3288 5132 7910 13509 13641
Size: 3288 1844 2778 5599 132
Fragments arranged by size:
5599 3288 2778 1844 132
SanDI GG'GwC_CC
Cuts at: 0 9318 13641
Size: 9318 4323
SapI GCTCTTCn'nnn_
Cuts at: 0 1360 2665 4957 5968 6694 7561 11350 13641
Size: 1360 1305 2292 1011 726 867 3789 2291
Fragments arranged by size:
3789 2292 2291 1360 1305 1011 867 726
SbfI CC_TGCA'GG
Cuts at: 0 1577 4607 4790 6800 8078 13641
Size: 1577 3030 183 2010 1278 5563
Fragments arranged by size:
5563 3030 2010 1577 1278 183
ScaI AGT'ACT
Cuts at: 0 9781 13641
Size: 9781 3860
SexAI A'CCwGG_T
Cuts at: 0 12902 13641
Size: 12902 739
SfcI C'TryA_G
Cuts at: 0 298 829 1201 1255 1280 1522 1573 1795
Size: 298 531 372 54 25 242 51 222
Cuts at: 1795 2098 2122 2221 2290 2880 2905 2980 3487
Size: 303 24 99 69 590 25 75 507
Cuts at: 3487 3667 3742 3966 4177 4264 4375 4423 4603
Size: 180 75 224 211 87 111 48 180
Cuts at: 4603 4627 4732 4786 4936 5215 5231 6106 6139
Size: 24 105 54 150 279 16 875 33
Cuts at: 6139 6193 6205 6401 6577 6625 6796 6994 7036
Size: 54 12 196 176 48 171 198 42
Cuts at: 7036 7405 7453 7471 7507 7717 7792 7972 8005
Size: 369 48 18 36 210 75 180 33
Cuts at: 8005 8074 8146 8593 8998 9540 9682 10051 10305
Size: 69 72 447 405 542 142 369 254
Cuts at: 10305 11725 12304 13234 13422 13431 13641
Size: 1420 579 930 188 9 210
Fragments arranged by size:
1420 930 875 590 579 542 531 507
447 405 372 369 369 303 298 279
254 242 224 222 211 210 210 198
196 188 180 180 180 176 171 150
142 111 105 99 87 75 75 75
72 69 69 54 54 54 51 48
48 48 42 36 33 33 25 25
24 24 18 16 12 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2359 2599 3355 3385 3895 4606 5404 6121
Size: 2359 240 756 30 510 711 798 717
Cuts at: 6121 6652 9260 13060 13114 13641
Size: 531 2608 3800 54 527
Fragments arranged by size:
3800 2608 2359 798 756 717 711 531
527 510 240 54 30
SfoI GGC'GCC
Cuts at: 0 1540 3660 4651 5947 6067 12601 13287 13641
Size: 1540 2120 991 1296 120 6534 686 354
Fragments arranged by size:
6534 2120 1540 1296 991 686 354 120
SgrAI Cr'CCGG_yG
Cuts at: 0 11416 12718 12976 13641
Size: 11416 1302 258 665
Fragments arranged by size:
11416 1302 665 258
SmaI CCC'GGG
Cuts at: 0 268 1759 6289 6382 8704 9379 13492 13641
Size: 268 1491 4530 93 2322 675 4113 149
Fragments arranged by size:
4530 4113 2322 1491 675 268 149 93
SmlI C'TyrA_G
Cuts at: 0 1063 1375 3262 3334 3373 3553 3604 3877
Size: 1063 312 1887 72 39 180 51 273
Cuts at: 3877 4906 5542 5566 6727 6934 6973 7153 7411
Size: 1029 636 24 1161 207 39 180 258
Cuts at: 7411 9991 10978 11029 11128 13324 13641
Size: 2580 987 51 99 2196 317
Fragments arranged by size:
2580 2196 1887 1161 1063 1029 987 636
317 312 273 258 207 180 180 99
72 51 51 39 39 24
SphI G_CATG'C
Cuts at: 0 4191 13641
Size: 4191 9450
SrfI GCCC'GGGC
Cuts at: 0 6382 13641
Size: 6382 7259
StuI AGG'CCT
Cuts at: 0 664 1679 2348 2803 2992 3388 5620 9538
Size: 664 1015 669 455 189 396 2232 3918
Cuts at: 9538 12469 12946 13063 13641
Size: 2931 477 117 578
Fragments arranged by size:
3918 2931 2232 1015 669 664 578 477
455 396 189 117
StyI C'CwwG_G
Cuts at: 0 399 479 992 1016 1823 2363 3383 3653
Size: 399 80 513 24 807 540 1020 270
Cuts at: 3653 3908 4304 5273 6047 6917 7574 8549 8666
Size: 255 396 969 774 870 657 975 117
Cuts at: 8666 9520 9533 10094 10526 10841 11678 11828 12111
Size: 854 13 561 432 315 837 150 283
Cuts at: 12111 12260 13412 13641
Size: 149 1152 229
Fragments arranged by size:
1152 1020 975 969 870 854 837 807
774 657 561 540 513 432 399 396
315 283 270 255 229 150 149 117
80 24 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1581 2408 3598 6607 7285 9718 12256 13641
Size: 1581 827 1190 3009 678 2433 2538 1385
Fragments arranged by size:
3009 2538 2433 1581 1385 1190 827 678
TatI w'GTAC_w
Cuts at: 0 1460 2327 3821 3995 9779 10670 13641
Size: 1460 867 1494 174 5784 891 2971
Fragments arranged by size:
5784 2971 1494 1460 891 867 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1090 1122 1873 1905 7289 7321 10729 10761
Size: 1090 32 751 32 5384 32 3408 32
Cuts at: 10761 11004 11036 12514 12546 13641
Size: 243 32 1478 32 1095
Fragments arranged by size:
5384 3408 1478 1095 1090 751 243 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 13003 13641
Size: 13003 638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1203 5361 7359 9948 10188 11685 12795 13641
Size: 1203 4158 1998 2589 240 1497 1110 846
Fragments arranged by size:
4158 2589 1998 1497 1203 1110 846 240
XhoI C'TCGA_G
Cuts at: 0 3373 3604 10978 11029 13641
Size: 3373 231 7374 51 2612
Fragments arranged by size:
7374 3373 2612 231 51
XmaI C'CCGG_G
Cuts at: 0 266 1757 6287 6380 8702 9377 13490 13641
Size: 266 1491 4530 93 2322 675 4113 151
Fragments arranged by size:
4530 4113 2322 1491 675 266 151 93
XmnI GAAnn'nnTTC
Cuts at: 0 139 13063 13641
Size: 139 12924 578
Fragments arranged by size:
12924 578 139
ZraI GAC'GTC
Cuts at: 0 8709 12389 13641
Size: 8709 3680 1252
Fragments arranged by size:
8709 3680 1252
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI HaeII Hin4I HincII HindIII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PflMI PfoI PmlI PpiI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuII SacI
SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgrAI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AclI AflII AscI AseI AsiSI AvrII BaeI BciVI
BfrBI BmtI BsrDI BstBI BstZ17I DraI FspAI HpaI
MfeI MluI NdeI NheI NruI NsiI PacI PciI
PmeI PsiI PvuI RsrII SalI SgfI SnaBI SpeI
SspI SwaI XbaI