Restriction map for
pFN21AE2699
(Linear) (Six-base) MAPSORT of: KIEE2699.seq Check: 8352 from: 1 to: 2754
KIEE2699.orf
With 182 enzymes: SgfI *
July 6, 2017 13:31 ..
AccI GT'mk_AC
Cuts at: 0 555 2754
Size: 555 2199
Acc65I G'GTAC_C
Cuts at: 0 1684 2754
Size: 1684 1070
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 2754
Size: 977 1777
AflII C'TTAA_G
Cuts at: 0 718 2754
Size: 718 2036
AflIII A'CryG_T
Cuts at: 0 866 2754
Size: 866 1888
AleI CACnn'nnGTG
Cuts at: 0 1606 2754
Size: 1606 1148
AlwNI CAG_nnn'CTG
Cuts at: 0 709 729 2754
Size: 709 20 2025
Fragments arranged by size:
2025 709 20
ApaI G_GGCC'C
Cuts at: 0 1518 2754
Size: 1518 1236
ApoI r'AATT_y
Cuts at: 0 1263 2566 2754
Size: 1263 1303 188
Fragments arranged by size:
1303 1263 188
AseI AT'TA_AT
Cuts at: 0 2704 2754
Size: 2704 50
AvaI C'yCGr_G
Cuts at: 0 28 922 2458 2754
Size: 28 894 1536 296
Fragments arranged by size:
1536 894 296 28
AvrII C'CTAG_G
Cuts at: 0 1650 2754
Size: 1650 1104
BamHI G'GATC_C
Cuts at: 0 2216 2754
Size: 2216 538
BanI G'GyrC_C
Cuts at: 0 1061 1684 2754
Size: 1061 623 1070
Fragments arranged by size:
1070 1061 623
BanII G_rGCy'C
Cuts at: 0 35 566 1518 1899 2754
Size: 35 531 952 381 855
Fragments arranged by size:
952 855 531 381 35
BbeI G_GCGC'C
Cuts at: 0 1065 2754
Size: 1065 1689
BbsI GAAGACnn'nnnn_
Cuts at: 0 539 2471 2754
Size: 539 1932 283
Fragments arranged by size:
1932 539 283
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 357 391 2754
Size: 34 323 34 2363
Fragments arranged by size:
2363 323 34 34
BclI T'GATC_A
Cuts at: 0 1088 2754
Size: 1088 1666
BglI GCCn_nnn'nGGC
Cuts at: 0 2008 2754
Size: 2008 746
Bme1580I G_kGCm'C
Cuts at: 0 60 1518 2487 2754
Size: 60 1458 969 267
Fragments arranged by size:
1458 969 267 60
BmgBI CAC'GTC
Cuts at: 0 1845 2754
Size: 1845 909
BmrI ACTGGGnnnn_n'
Cuts at: 0 1127 2754
Size: 1127 1627
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 1709 2754
Size: 564 1145 1045
Fragments arranged by size:
1145 1045 564
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 1170 2754
Size: 631 539 1584
Fragments arranged by size:
1584 631 539
BsaAI yAC'GTr
Cuts at: 0 867 1608 2754
Size: 867 741 1146
Fragments arranged by size:
1146 867 741
BsaBI GATnn'nnATC
Cuts at: 0 1163 2754
Size: 1163 1591
BsaHI Gr'CG_yC
Cuts at: 0 1062 2754
Size: 1062 1692
BsaWI w'CCGG_w
Cuts at: 0 1670 2754
Size: 1670 1084
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 273 303 2754
Size: 273 30 2451
Fragments arranged by size:
2451 273 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7 52 330 1580 2728 2754
Size: 7 45 278 1250 1148 26
Fragments arranged by size:
1250 1148 278 45 26 7
BseYI C'CCAG_C
Cuts at: 0 23 53 1016 2754
Size: 23 30 963 1738
Fragments arranged by size:
1738 963 30 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 2754
Size: 376 2378
BsiHKAI G_wGCw'C
Cuts at: 0 8 566 1748 1899 2231 2754
Size: 8 558 1182 151 332 523
Fragments arranged by size:
1182 558 523 332 151 8
BsmI GAATG_Cn'
Cuts at: 0 1661 2754
Size: 1661 1093
Bsp1286I G_dGCh'C
Cuts at: 0 8 35 60 566 1518 1748 1899 2231
Size: 8 27 25 506 952 230 151 332
Cuts at: 2231 2487 2754
Size: 256 267
Fragments arranged by size:
952 506 332 267 256 230 151 27
25 8
BspHI T'CATG_A
Cuts at: 0 1643 2168 2754
Size: 1643 525 586
Fragments arranged by size:
1643 586 525
BsrDI GCAATG_nn'
Cuts at: 0 375 995 2638 2754
Size: 375 620 1643 116
Fragments arranged by size:
1643 620 375 116
BsrGI T'GTAC_A
Cuts at: 0 983 1382 2754
Size: 983 399 1372
Fragments arranged by size:
1372 983 399
BstBI TT'CG_AA
Cuts at: 0 2727 2754
Size: 2727 27
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1362 1733 2487 2754
Size: 1362 371 754 267
Fragments arranged by size:
1362 754 371 267
BstYI r'GATC_y
Cuts at: 0 486 795 2216 2754
Size: 486 309 1421 538
Fragments arranged by size:
1421 538 486 309
BtgI C'CryG_G
Cuts at: 0 893 1057 2754
Size: 893 164 1697
Fragments arranged by size:
1697 893 164
BtsI GCAGTG_nn'
Cuts at: 0 1045 1589 2754
Size: 1045 544 1165
Fragments arranged by size:
1165 1045 544
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2250 2285 2754
Size: 2250 35 469
Fragments arranged by size:
2250 469 35
EaeI y'GGCC_r
Cuts at: 0 992 1331 2000 2754
Size: 992 339 669 754
Fragments arranged by size:
992 754 669 339
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 595 2754
Size: 595 2159
EcoICRI GAG'CTC
Cuts at: 0 564 1897 2754
Size: 564 1333 857
Fragments arranged by size:
1333 857 564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 977 1709 2754
Size: 564 413 732 1045
Fragments arranged by size:
1045 732 564 413
EcoO109I rG'GnC_Cy
Cuts at: 0 909 1514 2313 2754
Size: 909 605 799 441
Fragments arranged by size:
909 799 605 441
EcoRI G'AATT_C
Cuts at: 0 1263 2566 2754
Size: 1263 1303 188
Fragments arranged by size:
1303 1263 188
HaeII r_GCGC'y
Cuts at: 0 1065 2754
Size: 1065 1689
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 471 503 874 906 2754
Size: 471 32 371 32 1848
Fragments arranged by size:
1848 471 371 32 32
KasI G'GCGC_C
Cuts at: 0 1061 2754
Size: 1061 1693
KpnI G_GTAC'C
Cuts at: 0 1688 2754
Size: 1688 1066
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1292 1872 1932 2674 2754
Size: 1292 580 60 742 80
Fragments arranged by size:
1292 742 580 80 60
MscI TGG'CCA
Cuts at: 0 994 1333 2754
Size: 994 339 1421
Fragments arranged by size:
1421 994 339
MslI CAynn'nnrTG
Cuts at: 0 739 1606 2754
Size: 739 867 1148
Fragments arranged by size:
1148 867 739
MspA1I CmG'CkG
Cuts at: 0 53 297 401 612 709 1020 1769 2754
Size: 53 244 104 211 97 311 749 985
Fragments arranged by size:
985 749 311 244 211 104 97 53
NarI GG'CG_CC
Cuts at: 0 1062 2754
Size: 1062 1692
NcoI C'CATG_G
Cuts at: 0 893 1057 2754
Size: 893 164 1697
Fragments arranged by size:
1697 893 164
NdeI CA'TA_TG
Cuts at: 0 2420 2754
Size: 2420 334
NspI r_CATG'y
Cuts at: 0 290 2754
Size: 290 2464
PasI CC'CwG_GG
Cuts at: 0 2487 2754
Size: 2487 267
PfoI T'CCnGG_A
Cuts at: 0 482 2754
Size: 482 2272
PmlI CAC'GTG
Cuts at: 0 867 2754
Size: 867 1887
PspOMI G'GGCC_C
Cuts at: 0 1514 2754
Size: 1514 1240
PstI C_TGCA'G
Cuts at: 0 908 1040 2754
Size: 908 132 1714
Fragments arranged by size:
1714 908 132
PvuII CAG'CTG
Cuts at: 0 53 297 612 1020 1769 2754
Size: 53 244 315 408 749 985
Fragments arranged by size:
985 749 408 315 244 53
SacI G_AGCT'C
Cuts at: 0 566 1899 2754
Size: 566 1333 855
Fragments arranged by size:
1333 855 566
ScaI AGT'ACT
Cuts at: 0 107 754 2754
Size: 107 647 2000
Fragments arranged by size:
2000 647 107
SfcI C'TryA_G
Cuts at: 0 807 904 1036 2754
Size: 807 97 132 1718
Fragments arranged by size:
1718 807 132 97
SfoI GGC'GCC
Cuts at: 0 1063 2754
Size: 1063 1691
SmaI CCC'GGG
Cuts at: 0 30 2754
Size: 30 2724
SmlI C'TyrA_G
Cuts at: 0 646 718 1149 2754
Size: 646 72 431 1605
Fragments arranged by size:
1605 646 431 72
SpeI A'CTAG_T
Cuts at: 0 1491 2754
Size: 1491 1263
SrfI GCCC'GGGC
Cuts at: 0 30 2754
Size: 30 2724
SspI AAT'ATT
Cuts at: 0 591 2502 2754
Size: 591 1911 252
Fragments arranged by size:
1911 591 252
StyI C'CwwG_G
Cuts at: 0 893 1057 1650 2754
Size: 893 164 593 1104
Fragments arranged by size:
1104 893 593 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 763 930 1838 2754
Size: 763 167 908 916
Fragments arranged by size:
916 908 763 167
TatI w'GTAC_w
Cuts at: 0 105 752 983 1382 1773 2754
Size: 105 647 231 399 391 981
Fragments arranged by size:
981 647 399 391 231 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 273 305 856 888 997 1029 2754
Size: 273 32 551 32 109 32 1725
Fragments arranged by size:
1725 551 273 109 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 301 616 2754
Size: 301 315 2138
Fragments arranged by size:
2138 315 301
XmaI C'CCGG_G
Cuts at: 0 28 2754
Size: 28 2726
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AleI AlwNI ApaI
ApoI AseI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BclI BglI Bme1580I BmgBI BmrI BpmI
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI BstBI
BstXI BstYI BtgI BtsI CspCI EaeI EciI EcoICRI
Eco57MI EcoO109I EcoRI HaeII Hin4I KasI KpnI MmeI
MscI MslI MspA1I NarI NcoI NdeI NspI PasI
PfoI PmlI PspOMI PstI PvuII SacI ScaI SfcI
SfoI SmaI SmlI SpeI SrfI SspI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AlfI AloI
ApaLI AscI AsiSI BaeI BbvCI BciVI BfrBI BglII
BlpI BmtI BplI Bpu10I BsaI BsiEI BsiWI BsmBI
BspEI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII
BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI
EarI EcoNI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI MfeI MluI NaeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI
SphI StuI SwaI Tth111I XbaI XhoI XmnI ZraI