Restriction map for
pFN21AE2697
(Linear) (Six-base) MAPSORT of: KIEE2697.seq Check: 7066 from: 1 to: 1896
KIEE2697.orf
With 182 enzymes: SgfI *
June 16, 2017 16:55 ..
AccI GT'mk_AC
Cuts at: 0 1280 1419 1896
Size: 1280 139 477
Fragments arranged by size:
1280 477 139
Acc65I G'GTAC_C
Cuts at: 0 1194 1896
Size: 1194 702
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 1451 1502 1717 1896
Size: 866 585 51 215 179
Fragments arranged by size:
866 585 215 179 51
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 437 1896
Size: 437 1459
AlwNI CAG_nnn'CTG
Cuts at: 0 1510 1896
Size: 1510 386
ApoI r'AATT_y
Cuts at: 0 838 1005 1896
Size: 838 167 891
Fragments arranged by size:
891 838 167
AvaI C'yCGr_G
Cuts at: 0 648 1896
Size: 648 1248
BamHI G'GATC_C
Cuts at: 0 1726 1896
Size: 1726 170
BanI G'GyrC_C
Cuts at: 0 463 1194 1896
Size: 463 731 702
Fragments arranged by size:
731 702 463
BanII G_rGCy'C
Cuts at: 0 31 1896
Size: 31 1865
BbsI GAAGACnn'nnnn_
Cuts at: 0 410 1896
Size: 410 1486
BglI GCCn_nnn'nGGC
Cuts at: 0 117 430 1896
Size: 117 313 1466
Fragments arranged by size:
1466 313 117
BglII A'GATC_T
Cuts at: 0 337 681 1896
Size: 337 344 1215
Fragments arranged by size:
1215 344 337
Bme1580I G_kGCm'C
Cuts at: 0 1442 1896
Size: 1442 454
BmgBI CAC'GTC
Cuts at: 0 1636 1896
Size: 1636 260
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1543 1896
Size: 1543 353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1779 1896
Size: 646 1133 117
Fragments arranged by size:
1133 646 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1159 1896
Size: 1159 737
BsaAI yAC'GTr
Cuts at: 0 1690 1896
Size: 1690 206
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1651 1681 1896
Size: 1651 30 215
Fragments arranged by size:
1651 215 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 1598 1655 1896
Size: 19 1579 57 241
Fragments arranged by size:
1579 241 57 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 762 1896
Size: 762 1134
BsiHKAI G_wGCw'C
Cuts at: 0 543 1896
Size: 543 1353
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1514 1580 1896
Size: 1514 66 316
Fragments arranged by size:
1514 316 66
Bsp1286I G_dGCh'C
Cuts at: 0 31 543 1442 1896
Size: 31 512 899 454
Fragments arranged by size:
899 512 454 31
BspHI T'CATG_A
Cuts at: 0 69 132 657 1896
Size: 69 63 525 1239
Fragments arranged by size:
1239 525 69 63
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 366 784 1896
Size: 366 418 1112
Fragments arranged by size:
1112 418 366
BssSI C'ACGA_G
Cuts at: 0 1879 1896
Size: 1879 17
BstAPI GCAn_nnn'nTGC
Cuts at: 0 298 463 1492 1896
Size: 298 165 1029 404
Fragments arranged by size:
1029 404 298 165
BstYI r'GATC_y
Cuts at: 0 337 681 1726 1896
Size: 337 344 1045 170
Fragments arranged by size:
1045 344 337 170
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1212 1896
Size: 1212 684
BtsI GCAGTG_nn'
Cuts at: 0 749 818 1896
Size: 749 69 1078
Fragments arranged by size:
1078 749 69
DraI TTT'AAA
Cuts at: 0 450 1271 1896
Size: 450 821 625
Fragments arranged by size:
821 625 450
EarI CTCTTCn'nnn_
Cuts at: 0 980 1553 1608 1623 1896
Size: 980 573 55 15 273
Fragments arranged by size:
980 573 273 55 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 866 1451 1502 1717 1779 1896
Size: 646 220 585 51 215 62 117
Fragments arranged by size:
646 585 220 215 117 62 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 155 1896
Size: 155 1741
FspI TGC'GCA
Cuts at: 0 577 1579 1816 1896
Size: 577 1002 237 80
Fragments arranged by size:
1002 577 237 80
HaeII r_GCGC'y
Cuts at: 0 41 1896
Size: 41 1855
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1651 1683 1896
Size: 1651 32 213
Fragments arranged by size:
1651 213 32
HincII GTy'rAC
Cuts at: 0 1746 1896
Size: 1746 150
HindIII A'AGCT_T
Cuts at: 0 828 1896
Size: 828 1068
KpnI G_GTAC'C
Cuts at: 0 1198 1896
Size: 1198 698
MfeI C'AATT_G
Cuts at: 0 323 1896
Size: 323 1573
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1014 1573 1896
Size: 1014 559 323
Fragments arranged by size:
1014 559 323
MslI CAynn'nnrTG
Cuts at: 0 791 1896
Size: 791 1105
MspA1I CmG'CkG
Cuts at: 0 624 1896
Size: 624 1272
NdeI CA'TA_TG
Cuts at: 0 545 1896
Size: 545 1351
NspI r_CATG'y
Cuts at: 0 296 561 1896
Size: 296 265 1335
Fragments arranged by size:
1335 296 265
PfoI T'CCnGG_A
Cuts at: 0 1455 1896
Size: 1455 441
PsiI TTA'TAA
Cuts at: 0 164 1896
Size: 164 1732
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 50 82 845 877 1896
Size: 50 32 763 32 1019
Fragments arranged by size:
1019 763 50 32 32
PvuII CAG'CTG
Cuts at: 0 624 1896
Size: 624 1272
SfcI C'TryA_G
Cuts at: 0 155 1896
Size: 155 1741
SmlI C'TyrA_G
Cuts at: 0 648 1174 1896
Size: 648 526 722
Fragments arranged by size:
722 648 526
SspI AAT'ATT
Cuts at: 0 720 1896
Size: 720 1176
StyI C'CwwG_G
Cuts at: 0 865 1896
Size: 865 1031
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1102 1896
Size: 1102 794
TatI w'GTAC_w
Cuts at: 0 1108 1896
Size: 1108 788
Tth111I GACn'n_nGTC
Cuts at: 0 1451 1896
Size: 1451 445
XbaI T'CTAG_A
Cuts at: 0 762 1342 1896
Size: 762 580 554
Fragments arranged by size:
762 580 554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1245 1896
Size: 1245 651
XhoI C'TCGA_G
Cuts at: 0 648 1896
Size: 648 1248
XmnI GAAnn'nnTTC
Cuts at: 0 1476 1896
Size: 1476 420
Enzymes that do cut:
AccI Acc65I AcuI AlfI AlwNI ApoI AvaI BamHI
BanI BanII BbsI BglI BglII Bme1580I BmgBI BplI
BpmI BpuEI BsaAI BsaXI BseRI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BssSI BstAPI BstYI BtgZI BtsI
DraI EarI Eco57MI EcoNI FspI HaeII Hin4I HincII
HindIII KpnI MfeI MmeI MslI MspA1I NdeI NspI
PfoI PsiI PsrI PvuII SfcI SmlI SspI StyI
TaqII TatI Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI
BmrI BmtI Bpu10I BsaI BsaBI BsaHI BsaWI BseYI
BsiEI BsiWI BsmI BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoO109I
EcoRI EcoRV FalI FseI FspAI HpaI KasI MluI
MscI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TstI XmaI ZraI