Restriction map for
pFN21AE2694
(Linear) (Six-base) MAPSORT of: KIEE2694.seq Check: 6047 from: 1 to: 3747
KIEE2694.orf
With 182 enzymes: SgfI *
June 29, 2017 17:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 307 414 1582 3747
Size: 307 107 1168 2165
Fragments arranged by size:
2165 1168 307 107
AatII G_ACGT'C
Cuts at: 0 686 3747
Size: 686 3061
AccI GT'mk_AC
Cuts at: 0 797 3149 3747
Size: 797 2352 598
Fragments arranged by size:
2352 797 598
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 516 1954 2377 2551 2791 3352 3747
Size: 61 455 1438 423 174 240 561 395
Fragments arranged by size:
1438 561 455 423 395 240 174 61
AfeI AGC'GCT
Cuts at: 0 38 3747
Size: 38 3709
AflII C'TTAA_G
Cuts at: 0 2026 3747
Size: 2026 1721
AflIII A'CryG_T
Cuts at: 0 2723 3747
Size: 2723 1024
AgeI A'CCGG_T
Cuts at: 0 2320 3747
Size: 2320 1427
AleI CACnn'nnGTG
Cuts at: 0 1852 2321 3473 3747
Size: 1852 469 1152 274
Fragments arranged by size:
1852 1152 469 274
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 136 664 3747
Size: 136 528 3083
Fragments arranged by size:
3083 528 136
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 538 570 2710 2742 3747
Size: 538 32 2140 32 1005
Fragments arranged by size:
2140 1005 538 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 204 393 678 873 1171 1174 1713 2709
Size: 204 189 285 195 298 3 539 996
Cuts at: 2709 2829 3037 3330 3747
Size: 120 208 293 417
Fragments arranged by size:
996 539 417 298 293 285 208 204
195 189 120 3
ApaI G_GGCC'C
Cuts at: 0 622 1540 2891 3630 3747
Size: 622 918 1351 739 117
Fragments arranged by size:
1351 918 739 622 117
AvaI C'yCGr_G
Cuts at: 0 103 463 622 1482 1862 2038 2456 2855
Size: 103 360 159 860 380 176 418 399
Cuts at: 2855 2890 2969 3131 3603 3629 3747
Size: 35 79 162 472 26 118
Fragments arranged by size:
860 472 418 399 380 360 176 162
159 118 103 79 35 26
BamHI G'GATC_C
Cuts at: 0 3015 3267 3396 3747
Size: 3015 252 129 351
Fragments arranged by size:
3015 351 252 129
BanI G'GyrC_C
Cuts at: 0 3478 3747
Size: 3478 269
BanII G_rGCy'C
Cuts at: 0 554 622 908 1054 1187 1239 1310 1540
Size: 554 68 286 146 133 52 71 230
Cuts at: 1540 1642 1790 1847 1880 2891 3101 3630 3747
Size: 102 148 57 33 1011 210 529 117
Fragments arranged by size:
1011 554 529 286 230 210 148 146
133 117 102 71 68 57 52 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 2968 3747
Size: 2968 779
BbvCI CC'TCA_GC
Cuts at: 0 355 3170 3747
Size: 355 2815 577
Fragments arranged by size:
2815 577 355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2138 2172 3747
Size: 2138 34 1575
Fragments arranged by size:
2138 1575 34
BclI T'GATC_A
Cuts at: 0 599 3747
Size: 599 3148
BglI GCCn_nnn'nGGC
Cuts at: 0 173 1106 1864 2092 2572 2629 2929 3747
Size: 173 933 758 228 480 57 300 818
Fragments arranged by size:
933 818 758 480 300 228 173 57
BlpI GC'TnA_GC
Cuts at: 0 592 1641 3747
Size: 592 1049 2106
Fragments arranged by size:
2106 1049 592
Bme1580I G_kGCm'C
Cuts at: 0 575 622 1540 2634 2891 3280 3616 3630
Size: 575 47 918 1094 257 389 336 14
Cuts at: 3630 3747
Size: 117
Fragments arranged by size:
1094 918 575 389 336 257 117 47
14
BmgBI CAC'GTC
Cuts at: 0 3404 3747
Size: 3404 343
BmrI ACTGGGnnnn_n'
Cuts at: 0 1334 3267 3747
Size: 1334 1933 480
Fragments arranged by size:
1933 1334 480
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1758 2538 3747
Size: 1758 780 1209
Fragments arranged by size:
1758 1209 780
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 190 226 253 727 754 903 922
Size: 109 81 36 27 474 27 149 19
Cuts at: 922 964 1021 1261 1782 2003 2434 2518 2563
Size: 42 57 240 521 221 431 84 45
Cuts at: 2563 2590 2737 3202 3235 3333 3406 3475 3663
Size: 27 147 465 33 98 73 69 188
Cuts at: 3663 3747
Size: 84
Fragments arranged by size:
521 474 465 431 240 221 188 149
147 109 98 84 84 81 73 69
57 45 42 36 33 27 27 27
19
Bpu10I CC'TnA_GC
Cuts at: 0 355 752 1352 1759 3170 3323 3747
Size: 355 397 600 407 1411 153 424
Fragments arranged by size:
1411 600 424 407 397 355 153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3325 3747
Size: 3325 422
BsaI GGTCTCn'nnnn_
Cuts at: 0 695 2362 2539 3747
Size: 695 1667 177 1208
Fragments arranged by size:
1667 1208 695 177
BsaAI yAC'GTr
Cuts at: 0 271 372 474 579 1732 2409 3747
Size: 271 101 102 105 1153 677 1338
Fragments arranged by size:
1338 1153 677 271 105 102 101
BsaHI Gr'CG_yC
Cuts at: 0 281 488 683 1252 1753 1772 2327 2699
Size: 281 207 195 569 501 19 555 372
Cuts at: 2699 3152 3416 3747
Size: 453 264 331
Fragments arranged by size:
569 555 501 453 372 331 281 264
207 195 19
BsaWI w'CCGG_w
Cuts at: 0 2320 2786 3747
Size: 2320 466 961
Fragments arranged by size:
2320 961 466
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 538 568 1271 1301 2359 2389 2710 2740
Size: 538 30 703 30 1058 30 321 30
Cuts at: 2740 2893 2923 3053 3083 3747
Size: 153 30 130 30 664
Fragments arranged by size:
1058 703 664 538 321 153 130 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 760 781 784 1412 1532 1618 1972 2092
Size: 760 21 3 628 120 86 354 120
Cuts at: 2092 2107 2829 2851 2854 2917 3208 3541 3591
Size: 15 722 22 3 63 291 333 50
Cuts at: 3591 3747
Size: 156
Fragments arranged by size:
760 722 628 354 333 291 156 120
120 86 63 50 22 21 15 3
3
BseYI C'CCAG_C
Cuts at: 0 51 644 866 1141 1413 1532 1654 2213
Size: 51 593 222 275 272 119 122 559
Cuts at: 2213 2378 2702 3137 3281 3411 3747
Size: 165 324 435 144 130 336
Fragments arranged by size:
593 559 435 336 324 275 272 222
165 144 130 122 119 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 534 880 1554 2386 3747
Size: 217 317 346 674 832 1361
Fragments arranged by size:
1361 832 674 346 317 217
BsiEI CG_ry'CG
Cuts at: 0 2183 3747
Size: 2183 1564
BsiHKAI G_wGCw'C
Cuts at: 0 554 971 1739 1951 3747
Size: 554 417 768 212 1796
Fragments arranged by size:
1796 768 554 417 212
BsmI GAATG_Cn'
Cuts at: 0 997 2068 3747
Size: 997 1071 1679
Fragments arranged by size:
1679 1071 997
Bsp1286I G_dGCh'C
Cuts at: 0 554 575 622 908 971 1054 1187 1239
Size: 554 21 47 286 63 83 133 52
Cuts at: 1239 1310 1540 1642 1739 1790 1847 1880 1951
Size: 71 230 102 97 51 57 33 71
Cuts at: 1951 2634 2891 3101 3280 3616 3630 3747
Size: 683 257 210 179 336 14 117
Fragments arranged by size:
683 554 336 286 257 230 210 179
133 117 102 97 83 71 71 63
57 52 51 47 33 21 14
BspHI T'CATG_A
Cuts at: 0 323 542 3747
Size: 323 219 3205
Fragments arranged by size:
3205 323 219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 233 307 414 638 728 1582 2069 3747
Size: 233 74 107 224 90 854 487 1678
Fragments arranged by size:
1678 854 487 233 224 107 90 74
BsrBI CCG'CTC
Cuts at: 0 215 917 1017 2510 2553 3747
Size: 215 702 100 1493 43 1194
Fragments arranged by size:
1493 1194 702 215 100 43
BsrDI GCAATG_nn'
Cuts at: 0 2350 3747
Size: 2350 1397
BsrFI r'CCGG_y
Cuts at: 0 2320 3303 3747
Size: 2320 983 444
Fragments arranged by size:
2320 983 444
BssHII G'CGCG_C
Cuts at: 0 315 633 635 637 3747
Size: 315 318 2 2 3110
Fragments arranged by size:
3110 318 315 2 2
BssSI C'ACGA_G
Cuts at: 0 952 2047 3747
Size: 952 1095 1700
Fragments arranged by size:
1700 1095 952
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1171 1174 1713 2409 3747
Size: 1171 3 539 696 1338
Fragments arranged by size:
1338 1171 696 539 3
BstEII G'GTnAC_C
Cuts at: 0 582 3591 3747
Size: 582 3009 156
Fragments arranged by size:
3009 582 156
BstXI CCAn_nnnn'nTGG
Cuts at: 0 411 1239 1854 3475 3747
Size: 411 828 615 1621 272
Fragments arranged by size:
1621 828 615 411 272
BstYI r'GATC_y
Cuts at: 0 3015 3063 3267 3396 3747
Size: 3015 48 204 129 351
Fragments arranged by size:
3015 351 204 129 48
BtgI C'CryG_G
Cuts at: 0 74 258 1187 1403 1586 1629 1850 2570
Size: 74 184 929 216 183 43 221 720
Cuts at: 2570 2976 3011 3068 3205 3474 3747
Size: 406 35 57 137 269 273
Fragments arranged by size:
929 720 406 273 269 221 216 184
183 137 74 57 43 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 995 3263 3747
Size: 995 2268 484
Fragments arranged by size:
2268 995 484
BtsI GCAGTG_nn'
Cuts at: 0 1681 3747
Size: 1681 2066
ClaI AT'CG_AT
Cuts at: 0 2918 3747
Size: 2918 829
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1980 2015 3747
Size: 1980 35 1732
Fragments arranged by size:
1980 1732 35
DraIII CAC_nnn'GTG
Cuts at: 0 843 2409 3747
Size: 843 1566 1338
Fragments arranged by size:
1566 1338 843
EaeI y'GGCC_r
Cuts at: 0 974 2093 2126 2180 2573 2678 3041 3301
Size: 974 1119 33 54 393 105 363 260
Cuts at: 3301 3747
Size: 446
Fragments arranged by size:
1119 974 446 393 363 260 105 54
33
EagI C'GGCC_G
Cuts at: 0 2180 3747
Size: 2180 1567
EarI CTCTTCn'nnn_
Cuts at: 0 1903 3686 3747
Size: 1903 1783 61
Fragments arranged by size:
1903 1783 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2848 3747
Size: 2848 899
EcoICRI GAG'CTC
Cuts at: 0 552 3747
Size: 552 3195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 109 190 226 253 516 727 754
Size: 61 48 81 36 27 263 211 27
Cuts at: 754 903 922 964 1021 1261 1782 1954 2003
Size: 149 19 42 57 240 521 172 49
Cuts at: 2003 2377 2434 2518 2551 2563 2590 2737 2791
Size: 374 57 84 33 12 27 147 54
Cuts at: 2791 3202 3235 3333 3352 3406 3475 3663 3747
Size: 411 33 98 19 54 69 188 84
Fragments arranged by size:
521 411 374 263 240 211 188 172
149 147 98 84 84 81 69 61
57 57 54 54 49 48 42 36
33 33 27 27 27 19 19 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1060 2692 3494 3747
Size: 1060 1632 802 253
Fragments arranged by size:
1632 1060 802 253
EcoO109I rG'GnC_Cy
Cuts at: 0 390 619 627 888 939 1356 1536 1537
Size: 390 229 8 261 51 417 180 1
Cuts at: 1537 1650 2460 2751 2887 3141 3296 3327 3626
Size: 113 810 291 136 254 155 31 299
Cuts at: 3626 3747
Size: 121
Fragments arranged by size:
810 417 390 299 291 261 254 229
180 155 136 121 113 51 31 8
1
FspI TGC'GCA
Cuts at: 0 160 1687 3747
Size: 160 1527 2060
Fragments arranged by size:
2060 1527 160
HaeII r_GCGC'y
Cuts at: 0 40 168 241 2102 2604 3747
Size: 40 128 73 1861 502 1143
Fragments arranged by size:
1861 1143 502 128 73 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2359 2391 3388 3420 3747
Size: 2359 32 997 32 327
Fragments arranged by size:
2359 997 327 32 32
HincII GTy'rAC
Cuts at: 0 144 798 3747
Size: 144 654 2949
Fragments arranged by size:
2949 654 144
HindIII A'AGCT_T
Cuts at: 0 2536 3747
Size: 2536 1211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 428 1745 1976 2549 3170 3747
Size: 302 126 1317 231 573 621 577
Fragments arranged by size:
1317 621 577 573 302 231 126
MscI TGG'CCA
Cuts at: 0 976 2095 2128 3043 3747
Size: 976 1119 33 915 704
Fragments arranged by size:
1119 976 915 704 33
MslI CAynn'nnrTG
Cuts at: 0 541 1852 2321 3473 3747
Size: 541 1311 469 1152 274
Fragments arranged by size:
1311 1152 541 469 274
MspA1I CmG'CkG
Cuts at: 0 260 308 648 675 732 927 1171 2145
Size: 260 48 340 27 57 195 244 974
Cuts at: 2145 2217 2259 2277 2451 2709 2829 2955 2978
Size: 72 42 18 174 258 120 126 23
Cuts at: 2978 3747
Size: 769
Fragments arranged by size:
974 769 340 260 258 244 195 174
126 120 72 57 48 42 27 23
18
NcoI C'CATG_G
Cuts at: 0 1403 1629 1850 3011 3747
Size: 1403 226 221 1161 736
Fragments arranged by size:
1403 1161 736 226 221
NotI GC'GGCC_GC
Cuts at: 0 2180 3747
Size: 2180 1567
NspI r_CATG'y
Cuts at: 0 2163 3747
Size: 2163 1584
PasI CC'CwG_GG
Cuts at: 0 3623 3747
Size: 3623 124
PflMI CCAn_nnn'nTGG
Cuts at: 0 393 1635 2412 3288 3747
Size: 393 1242 777 876 459
Fragments arranged by size:
1242 876 777 459 393
PfoI T'CCnGG_A
Cuts at: 0 203 2582 2747 3679 3747
Size: 203 2379 165 932 68
Fragments arranged by size:
2379 932 203 165 68
PmlI CAC'GTG
Cuts at: 0 474 1732 2409 3747
Size: 474 1258 677 1338
Fragments arranged by size:
1338 1258 677 474
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 538 570 2710 2742 3747
Size: 538 32 2140 32 1005
Fragments arranged by size:
2140 1005 538 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 627 1650 2460 2751 3141 3747
Size: 627 1023 810 291 390 606
Fragments arranged by size:
1023 810 627 606 390 291
PspOMI G'GGCC_C
Cuts at: 0 618 1536 2887 3626 3747
Size: 618 918 1351 739 121
Fragments arranged by size:
1351 918 739 618 121
PstI C_TGCA'G
Cuts at: 0 293 674 887 1579 1830 2450 3060 3369
Size: 293 381 213 692 251 620 610 309
Cuts at: 3369 3495 3747
Size: 126 252
Fragments arranged by size:
692 620 610 381 309 293 252 251
213 126
PvuII CAG'CTG
Cuts at: 0 308 648 675 732 927 1171 2145 2217
Size: 308 340 27 57 195 244 974 72
Cuts at: 2217 2259 2277 2451 2709 2829 2955 3747
Size: 42 18 174 258 120 126 792
Fragments arranged by size:
974 792 340 308 258 244 195 174
126 120 72 57 42 27 18
SacI G_AGCT'C
Cuts at: 0 554 3747
Size: 554 3193
SacII CC_GC'GG
Cuts at: 0 261 2979 3747
Size: 261 2718 768
Fragments arranged by size:
2718 768 261
SalI G'TCGA_C
Cuts at: 0 796 3747
Size: 796 2951
SanDI GG'GwC_CC
Cuts at: 0 1650 3747
Size: 1650 2097
SapI GCTCTTCn'nnn_
Cuts at: 0 1903 3747
Size: 1903 1844
SbfI CC_TGCA'GG
Cuts at: 0 3060 3747
Size: 3060 687
SfcI C'TryA_G
Cuts at: 0 289 670 883 1575 1826 2446 3056 3365
Size: 289 381 213 692 251 620 610 309
Cuts at: 3365 3491 3747
Size: 126 256
Fragments arranged by size:
692 620 610 381 309 289 256 251
213 126
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1864 3747
Size: 1864 1883
SgrAI Cr'CCGG_yG
Cuts at: 0 2320 3747
Size: 2320 1427
SmaI CCC'GGG
Cuts at: 0 1484 1864 2458 2892 2971 3133 3631 3747
Size: 1484 380 594 434 79 162 498 116
Fragments arranged by size:
1484 594 498 434 380 162 116 79
SmlI C'TyrA_G
Cuts at: 0 2026 3340 3747
Size: 2026 1314 407
Fragments arranged by size:
2026 1314 407
SphI G_CATG'C
Cuts at: 0 2163 3747
Size: 2163 1584
StuI AGG'CCT
Cuts at: 0 1394 3747
Size: 1394 2353
StyI C'CwwG_G
Cuts at: 0 1403 1629 1838 1850 1880 2207 3011 3287
Size: 1403 226 209 12 30 327 804 276
Cuts at: 3287 3392 3747
Size: 105 355
Fragments arranged by size:
1403 804 355 327 276 226 209 105
30 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 658 1062 1692 2563 3610 3747
Size: 658 404 630 871 1047 137
Fragments arranged by size:
1047 871 658 630 404 137
TatI w'GTAC_w
Cuts at: 0 347 2591 3747
Size: 347 2244 1156
Fragments arranged by size:
2244 1156 347
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1271 1303 2893 2925 3053 3069 3085 3101
Size: 1271 32 1590 32 128 16 16 16
Cuts at: 3101 3252 3259 3284 3291 3747
Size: 151 7 25 7 456
Fragments arranged by size:
1590 1271 456 151 128 32 32 25
16 16 16 7 7
Tth111I GACn'n_nGTC
Cuts at: 0 2373 3463 3747
Size: 2373 1090 284
Fragments arranged by size:
2373 1090 284
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1143 1627 2001 2214 3036 3408 3747
Size: 1143 484 374 213 822 372 339
Fragments arranged by size:
1143 822 484 374 372 339 213
XmaI C'CCGG_G
Cuts at: 0 1482 1862 2456 2890 2969 3131 3629 3747
Size: 1482 380 594 434 79 162 498 118
Fragments arranged by size:
1482 594 498 434 380 162 118 79
XmnI GAAnn'nnTTC
Cuts at: 0 2743 2947 3695 3747
Size: 2743 204 748 52
Fragments arranged by size:
2743 748 204 52
ZraI GAC'GTC
Cuts at: 0 684 3747
Size: 684 3063
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BclI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII HindIII
MmeI MscI MslI MspA1I NcoI NotI NspI PasI
PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII
SacI SacII SalI SanDI SapI SbfI SfcI SfiI
SgrAI SmaI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AhdI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BciVI BfrBI BglII BmtI BsaBI
BsiWI BsmBI BspEI BsrGI BstBI BstZ17I Bsu36I DraI
DrdI EcoRI EcoRV FalI FseI FspAI HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NruI NsiI PacI PciI PmeI PshAI PsiI PspXI
PsrI PvuI RsrII ScaI SexAI SfoI SgfI SnaBI
SpeI SrfI SspI SwaI XbaI XhoI