Restriction map for
pFN21AE2693
(Linear) (Six-base) MAPSORT of: KIEE2693.seq Check: 9189 from: 1 to: 11022
KIEE2693.orf
With 182 enzymes: SgfI *
August 25, 2017 13:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1329 1572 3109 4713 5889 7955 8798 8957
Size: 1329 243 1537 1604 1176 2066 843 159
Cuts at: 8957 10643 11022
Size: 1686 379
Fragments arranged by size:
2066 1686 1604 1537 1329 1176 843 379
243 159
AccI GT'mk_AC
Cuts at: 0 11004 11022
Size: 11004 18
Acc65I G'GTAC_C
Cuts at: 0 7064 10594 11022
Size: 7064 3530 428
Fragments arranged by size:
7064 3530 428
AclI AA'CG_TT
Cuts at: 0 7767 11022
Size: 7767 3255
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 1540 1984 2983 3271 3598 5959 6304
Size: 880 660 444 999 288 327 2361 345
Cuts at: 6304 6355 6535 6574 6608 7611 7986 8599 8679
Size: 51 180 39 34 1003 375 613 80
Cuts at: 8679 9113 10351 10504 11022
Size: 434 1238 153 518
Fragments arranged by size:
2361 1238 1003 999 880 660 613 518
444 434 375 345 327 288 180 153
80 51 39 34
AfeI AGC'GCT
Cuts at: 0 802 11022
Size: 802 10220
AflII C'TTAA_G
Cuts at: 0 1324 10830 11022
Size: 1324 9506 192
Fragments arranged by size:
9506 1324 192
AflIII A'CryG_T
Cuts at: 0 4101 7098 7100 11022
Size: 4101 2997 2 3922
Fragments arranged by size:
4101 3922 2997 2
AleI CACnn'nnGTG
Cuts at: 0 586 1816 5851 7931 11022
Size: 586 1230 4035 2080 3091
Fragments arranged by size:
4035 3091 2080 1230 586
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 728 3031 3857 5869 10262 11022
Size: 728 2303 826 2012 4393 760
Fragments arranged by size:
4393 2303 2012 826 760 728
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 65 97 10445 10477 11022
Size: 65 32 10348 32 545
Fragments arranged by size:
10348 545 65 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 744 762 1032 1222 1239 1404 1455 1692
Size: 744 18 270 190 17 165 51 237
Cuts at: 1692 1869 1914 2711 3216 3246 3339 3417 3480
Size: 177 45 797 505 30 93 78 63
Cuts at: 3480 3576 3609 3930 4350 4368 4809 4863 5442
Size: 96 33 321 420 18 441 54 579
Cuts at: 5442 5640 5682 5712 5895 6513 6687 6696 6750
Size: 198 42 30 183 618 174 9 54
Cuts at: 6750 6858 7167 7203 7464 7605 7650 7671 7725
Size: 108 309 36 261 141 45 21 54
Cuts at: 7725 8217 8238 8454 8772 8811 8928 9012 9408
Size: 492 21 216 318 39 117 84 396
Cuts at: 9408 9468 9664 9927 9975 10005 10116 10137 10191
Size: 60 196 263 48 30 111 21 54
Cuts at: 10191 10212 10284 10482 10635 10670 10929 11022
Size: 21 72 198 153 35 259 93
Fragments arranged by size:
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396 321 318 309 270 263 261 259
237 216 198 198 196 190 183 177
174 165 153 141 117 111 108 96
93 93 84 78 72 63 60 54
54 54 54 51 48 45 45 42
39 36 35 33 30 30 30 21
21 21 21 18 18 17 9
ApaI G_GGCC'C
Cuts at: 0 1890 1899 2507 2649 2670 3285 4323 4857
Size: 1890 9 608 142 21 615 1038 534
Cuts at: 4857 5811 6507 7377 10230 11022
Size: 954 696 870 2853 792
Fragments arranged by size:
2853 1890 1038 954 870 792 696 615
608 534 142 21 9
ApaLI G'TGCA_C
Cuts at: 0 334 7237 9524 10974 11022
Size: 334 6903 2287 1450 48
Fragments arranged by size:
6903 2287 1450 334 48
ApoI r'AATT_y
Cuts at: 0 8172 11022
Size: 8172 2850
AvaI C'yCGr_G
Cuts at: 0 23 34 312 769 2146 2195 2438 3785
Size: 23 11 278 457 1377 49 243 1347
Cuts at: 3785 5287 5483 5981 6353 6662 7109 7303 7942
Size: 1502 196 498 372 309 447 194 639
Cuts at: 7942 8846 8915 9958 10489 11022
Size: 904 69 1043 531 533
Fragments arranged by size:
1502 1377 1347 1043 904 639 533 531
498 457 447 372 309 278 243 196
194 69 49 23 11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 7087 7120 11022
Size: 7087 33 3902
Fragments arranged by size:
7087 3902 33
BamHI G'GATC_C
Cuts at: 0 2142 6491 6809 9034 11022
Size: 2142 4349 318 2225 1988
Fragments arranged by size:
4349 2225 2142 1988 318
BanI G'GyrC_C
Cuts at: 0 47 384 557 1810 2026 2354 3287 3534
Size: 47 337 173 1253 216 328 933 247
Cuts at: 3534 4379 4660 4769 5659 6347 7064 7619 7937
Size: 845 281 109 890 688 717 555 318
Cuts at: 7937 7967 8786 9508 9764 10594 11022
Size: 30 819 722 256 830 428
Fragments arranged by size:
1253 933 890 845 830 819 722 717
688 555 428 337 328 318 281 256
247 216 173 109 47 30
BanII G_rGCy'C
Cuts at: 0 32 287 363 1362 1761 1890 1899 2476
Size: 32 255 76 999 399 129 9 577
Cuts at: 2476 2507 2649 2670 2723 3091 3285 4022 4244
Size: 31 142 21 53 368 194 737 222
Cuts at: 4244 4323 4333 4598 4694 4857 5041 5811 6078
Size: 79 10 265 96 163 184 770 267
Cuts at: 6078 6238 6507 7377 7473 7489 7750 7838 8042
Size: 160 269 870 96 16 261 88 204
Cuts at: 8042 8414 8436 8474 8642 9023 9671 10230 10463
Size: 372 22 38 168 381 648 559 233
Cuts at: 10463 10782 10905 10936 11022
Size: 319 123 31 86
Fragments arranged by size:
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381 372 368 319 269 267 265 261
255 233 222 204 194 184 168 163
160 142 129 123 96 96 88 86
79 76 53 38 32 31 31 22
21 16 10 9
BbeI G_GCGC'C
Cuts at: 0 2030 2358 3291 6351 9768 11022
Size: 2030 328 933 3060 3417 1254
Fragments arranged by size:
3417 3060 2030 1254 933 328
BbsI GAAGACnn'nnnn_
Cuts at: 0 178 192 574 3136 6270 6343 7793 9697
Size: 178 14 382 2562 3134 73 1450 1904
Cuts at: 9697 11022
Size: 1325
Fragments arranged by size:
3134 2562 1904 1450 1325 382 178 73
14
BbvCI CC'TCA_GC
Cuts at: 0 2057 2113 2350 2852 3421 5216 6482 8092
Size: 2057 56 237 502 569 1795 1266 1610
Cuts at: 8092 8528 10028 10295 11022
Size: 436 1500 267 727
Fragments arranged by size:
2057 1795 1610 1500 1266 727 569 502
436 267 237 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 6244 6278 9229 9263 10255 10289 11022
Size: 6244 34 2951 34 992 34 733
Fragments arranged by size:
6244 2951 992 733 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3883 11022
Size: 3883 7139
BglI GCCn_nnn'nGGC
Cuts at: 0 218 1074 1099 1375 1894 2392 2531 3280
Size: 218 856 25 276 519 498 139 749
Cuts at: 3280 4423 4441 4465 5872 6583 8266 8362 8524
Size: 1143 18 24 1407 711 1683 96 162
Cuts at: 8524 8668 9103 9833 10306 10808 11022
Size: 144 435 730 473 502 214
Fragments arranged by size:
1683 1407 1143 856 749 730 711 519
502 498 473 435 276 218 214 162
144 139 96 25 24 18
BglII A'GATC_T
Cuts at: 0 476 2088 5532 7182 11022
Size: 476 1612 3444 1650 3840
Fragments arranged by size:
3840 3444 1650 1612 476
BlpI GC'TnA_GC
Cuts at: 0 734 755 1606 1778 2567 2870 3368 3410
Size: 734 21 851 172 789 303 498 42
Cuts at: 3410 7672 9047 9665 10046 11022
Size: 4262 1375 618 381 976
Fragments arranged by size:
4262 1375 976 851 789 734 618 498
381 303 172 42 21
Bme1580I G_kGCm'C
Cuts at: 0 50 338 1813 1890 1899 2507 2649 2670
Size: 50 288 1475 77 9 608 142 21
Cuts at: 2670 3285 3539 3807 4323 4665 4772 4857 5142
Size: 615 254 268 516 342 107 85 285
Cuts at: 5142 5405 5662 5811 5826 5952 6117 6507 7241
Size: 263 257 149 15 126 165 390 734
Cuts at: 7241 7377 7622 7940 7959 8482 8863 8891 9296
Size: 136 245 318 19 523 381 28 405
Cuts at: 9296 9528 9990 10230 10349 10978 11022
Size: 232 462 240 119 629 44
Fragments arranged by size:
1475 734 629 615 608 523 516 462
405 390 381 342 318 288 285 268
263 257 254 245 240 232 165 149
142 136 126 119 107 85 77 50
44 28 21 19 15 9
BmgBI CAC'GTC
Cuts at: 0 777 3054 3717 10035 11022
Size: 777 2277 663 6318 987
Fragments arranged by size:
6318 2277 987 777 663
BmrI ACTGGGnnnn_n'
Cuts at: 0 83 921 1185 4830 4851 6717 7604 8873
Size: 83 838 264 3645 21 1866 887 1269
Cuts at: 8873 9841 11022
Size: 968 1181
Fragments arranged by size:
3645 1866 1269 1181 968 887 838 264
83 21
BmtI G_CTAG'C
Cuts at: 0 9232 9982 11022
Size: 9232 750 1040
Fragments arranged by size:
9232 1040 750
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 306 1650 1815 3073 3230 6658 6677 7508
Size: 306 1344 165 1258 157 3428 19 831
Cuts at: 7508 7575 11022
Size: 67 3447
Fragments arranged by size:
3447 3428 1344 1258 831 306 165 157
67 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 364 636 886 1006 1228 1435 1666
Size: 301 63 272 250 120 222 207 231
Cuts at: 1666 1753 1924 2173 2323 2389 2431 2462 2529
Size: 87 171 249 150 66 42 31 67
Cuts at: 2529 2587 2707 2755 2806 2974 3046 3088 3163
Size: 58 120 48 51 168 72 42 75
Cuts at: 3163 3463 3643 3880 3925 3931 4228 4324 4341
Size: 300 180 237 45 6 297 96 17
Cuts at: 4341 4411 4525 4678 4852 4933 5055 5197 5632
Size: 70 114 153 174 81 122 142 435
Cuts at: 5632 5662 5707 5836 5995 6202 6772 6793 6880
Size: 30 45 129 159 207 570 21 87
Cuts at: 6880 7207 7246 7408 7747 7882 7894 7924 8053
Size: 327 39 162 339 135 12 30 129
Cuts at: 8053 8305 8332 8416 8428 8515 8686 8833 8950
Size: 252 27 84 12 87 171 147 117
Cuts at: 8950 9046 9142 9214 9349 9430 9535 9655 9790
Size: 96 96 72 135 81 105 120 135
Cuts at: 9790 10234 10306 10647 11022
Size: 444 72 341 375
Fragments arranged by size:
570 444 435 375 341 339 327 301
300 297 272 252 250 249 237 231
222 207 207 180 174 171 171 168
162 159 153 150 147 142 135 135
135 129 129 122 120 120 120 117
114 105 96 96 96 87 87 87
84 81 81 75 72 72 72 70
67 66 63 58 51 48 45 45
42 42 39 31 30 30 27 21
17 12 12 6
Bpu10I CC'TnA_GC
Cuts at: 0 2057 2113 2350 2470 2852 3421 3730 3769
Size: 2057 56 237 120 382 569 309 39
Cuts at: 3769 4582 5093 5216 6482 8092 8528 8993 10028
Size: 813 511 123 1266 1610 436 465 1035
Cuts at: 10028 10295 11022
Size: 267 727
Fragments arranged by size:
2057 1610 1266 1035 813 727 569 511
465 436 382 309 267 237 123 120
56 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1724 2640 4039 6955 11022
Size: 1724 916 1399 2916 4067
Fragments arranged by size:
4067 2916 1724 1399 916
BsaI GGTCTCn'nnnn_
Cuts at: 0 419 2335 4474 5503 6213 6681 9131 9331
Size: 419 1916 2139 1029 710 468 2450 200
Cuts at: 9331 11022
Size: 1691
Fragments arranged by size:
2450 2139 1916 1691 1029 710 468 419
200
BsaAI yAC'GTr
Cuts at: 0 2361 4104 7101 11022
Size: 2361 1743 2997 3921
Fragments arranged by size:
3921 2997 2361 1743
BsaHI Gr'CG_yC
Cuts at: 0 2027 2355 2443 3288 6348 8702 9353 9765
Size: 2027 328 88 845 3060 2354 651 412
Cuts at: 9765 10009 11022
Size: 244 1013
Fragments arranged by size:
3060 2354 2027 1013 845 651 412 328
244 88
BsaWI w'CCGG_w
Cuts at: 0 230 4024 4496 7061 7364 11022
Size: 230 3794 472 2565 303 3658
Fragments arranged by size:
3794 3658 2565 472 303 230
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 664 694 826 856 1748 1778 2296 2326
Size: 664 30 132 30 892 30 518 30
Cuts at: 2326 2710 2740 4137 4167 4339 4369 5023 5053
Size: 384 30 1397 30 172 30 654 30
Cuts at: 5053 5851 5881 5977 6007 6872 6902 6936 6966
Size: 798 30 96 30 865 30 34 30
Cuts at: 6966 9994 10024 10445 10475 10810 10840 11022
Size: 3028 30 421 30 335 30 182
Fragments arranged by size:
3028 1397 892 865 798 664 654 518
421 384 335 182 172 132 96 34
30 30 30 30 30 30 30 30
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 517 888 1663 1791 1960 1963 2428
Size: 22 495 371 775 128 169 3 465
Cuts at: 2428 2692 3049 3162 3202 3460 3918 3930 3933
Size: 264 357 113 40 258 458 12 3
Cuts at: 3933 4942 5062 5920 6199 6619 7234 7484 8050
Size: 1009 120 858 279 420 615 250 566
Cuts at: 8050 8134 8302 8671 9337 9573 10033 10231 11022
Size: 84 168 369 666 236 460 198 791
Fragments arranged by size:
1009 858 791 775 666 615 566 495
465 460 458 420 371 369 357 279
264 258 250 236 198 169 168 128
120 113 84 40 22 12 3 3
BseYI C'CCAG_C
Cuts at: 0 64 627 744 1196 1404 1499 1853 2562
Size: 64 563 117 452 208 95 354 709
Cuts at: 2562 3837 4919 5810 6008 6012 6506 6680 8256
Size: 1275 1082 891 198 4 494 174 1576
Cuts at: 8256 8292 8538 8856 8884 9002 9233 10074 10256
Size: 36 246 318 28 118 231 841 182
Cuts at: 10256 10313 11022
Size: 57 709
Fragments arranged by size:
1576 1275 1082 891 841 709 709 563
494 452 354 318 246 231 208 198
182 174 118 117 95 64 57 36
28 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 2785 3001 3027 3676 3827 4549 5131
Size: 688 2097 216 26 649 151 722 582
Cuts at: 5131 5284 5292 5725 5761 7216 7900 7909 8746
Size: 153 8 433 36 1455 684 9 837
Cuts at: 8746 8899 8941 9481 9895 9923 10360 11022
Size: 153 42 540 414 28 437 662
Fragments arranged by size:
2097 1455 837 722 688 684 662 649
582 540 437 433 414 216 153 153
151 42 36 28 26 9 8
BsiEI CG_ry'CG
Cuts at: 0 2336 2396 4939 6177 6914 11022
Size: 2336 60 2543 1238 737 4108
Fragments arranged by size:
4108 2543 2336 1238 737 60
BsiHKAI G_wGCw'C
Cuts at: 0 32 287 338 363 2723 3059 3091 3431
Size: 32 255 51 25 2360 336 32 340
Cuts at: 3431 3775 4244 4333 4598 4616 4694 5041 5294
Size: 344 469 89 265 18 78 347 253
Cuts at: 5294 6238 6866 7241 7750 7902 8042 8301 8474
Size: 944 628 375 509 152 140 259 173
Cuts at: 8474 9023 9528 9671 10463 10557 10978 11022
Size: 549 505 143 792 94 421 44
Fragments arranged by size:
2360 944 792 628 549 509 505 469
421 375 347 344 340 336 265 259
255 253 173 152 143 140 94 89
78 51 44 32 32 25 18
BsmI GAATG_Cn'
Cuts at: 0 1039 1995 11022
Size: 1039 956 9027
Fragments arranged by size:
9027 1039 956
BsmBI CGTCTCn'nnnn_
Cuts at: 0 115 825 3044 4961 10561 11022
Size: 115 710 2219 1917 5600 461
Fragments arranged by size:
5600 2219 1917 710 461 115
Bsp1286I G_dGCh'C
Cuts at: 0 32 50 287 338 363 1362 1761 1813
Size: 32 18 237 51 25 999 399 52
Cuts at: 1813 1890 1899 2476 2507 2649 2670 2723 3059
Size: 77 9 577 31 142 21 53 336
Cuts at: 3059 3091 3285 3431 3539 3775 3807 4022 4244
Size: 32 194 146 108 236 32 215 222
Cuts at: 4244 4323 4333 4598 4616 4665 4694 4772 4857
Size: 79 10 265 18 49 29 78 85
Cuts at: 4857 5041 5142 5294 5405 5662 5811 5826 5952
Size: 184 101 152 111 257 149 15 126
Cuts at: 5952 6078 6117 6238 6507 6866 7241 7377 7473
Size: 126 39 121 269 359 375 136 96
Cuts at: 7473 7489 7622 7750 7838 7902 7940 7959 8042
Size: 16 133 128 88 64 38 19 83
Cuts at: 8042 8301 8414 8436 8474 8482 8642 8863 8891
Size: 259 113 22 38 8 160 221 28
Cuts at: 8891 9023 9296 9528 9671 9990 10230 10349 10463
Size: 132 273 232 143 319 240 119 114
Cuts at: 10463 10557 10782 10905 10936 10978 11022
Size: 94 225 123 31 42 44
Fragments arranged by size:
999 577 399 375 359 336 319 273
269 265 259 257 240 237 236 232
225 222 221 215 194 184 160 152
149 146 143 142 136 133 132 128
126 126 123 121 119 114 113 111
108 101 96 94 88 85 83 79
78 77 64 53 52 51 49 44
42 39 38 38 32 32 32 31
31 29 28 25 22 21 19 18
18 16 15 10 9 8
BspEI T'CCGG_A
Cuts at: 0 230 4024 4496 7364 11022
Size: 230 3794 472 2868 3658
Fragments arranged by size:
3794 3658 2868 472 230
BspHI T'CATG_A
Cuts at: 0 809 1161 6611 11022
Size: 809 352 5450 4411
Fragments arranged by size:
5450 4411 809 352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1329 1572 1736 3109 3446 4110 4358 4713
Size: 1329 243 164 1373 337 664 248 355
Cuts at: 4713 5231 5889 5903 7955 8798 8957 10124 10643
Size: 518 658 14 2052 843 159 1167 519
Cuts at: 10643 11022
Size: 379
Fragments arranged by size:
2052 1373 1329 1167 843 664 658 519
518 379 355 337 248 243 164 159
14
BsrBI CCG'CTC
Cuts at: 0 2289 5667 5820 6330 7230 7492 9051 9168
Size: 2289 3378 153 510 900 262 1559 117
Cuts at: 9168 9519 11022
Size: 351 1503
Fragments arranged by size:
3378 2289 1559 1503 900 510 351 262
153 117
BsrDI GCAATG_nn'
Cuts at: 0 749 6734 7436 11022
Size: 749 5985 702 3586
Fragments arranged by size:
5985 3586 749 702
BsrFI r'CCGG_y
Cuts at: 0 1662 5369 6365 6522 11022
Size: 1662 3707 996 157 4500
Fragments arranged by size:
4500 3707 1662 996 157
BsrGI T'GTAC_A
Cuts at: 0 239 11022
Size: 239 10783
BssHII G'CGCG_C
Cuts at: 0 2382 4030 11022
Size: 2382 1648 6992
Fragments arranged by size:
6992 2382 1648
BssSI C'ACGA_G
Cuts at: 0 2092 5571 5653 6271 7216 7482 8833 8902
Size: 2092 3479 82 618 945 266 1351 69
Cuts at: 8902 11022
Size: 2120
Fragments arranged by size:
3479 2120 2092 1351 945 618 266 82
69
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1222 1602 1728 1914 2748 3246 3609 4350
Size: 1222 380 126 186 834 498 363 741
Cuts at: 4350 4734 5298 5442 5682 6031 6696 6870 7203
Size: 384 564 144 240 349 665 174 333
Cuts at: 7203 7443 7605 7857 8217 9300 10635 10638 11022
Size: 240 162 252 360 1083 1335 3 384
Fragments arranged by size:
1335 1222 1083 834 741 665 564 498
384 384 380 363 360 349 333 252
240 240 186 174 162 144 126 3
BstEII G'GTnAC_C
Cuts at: 0 6841 11022
Size: 6841 4181
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1848 2507 4252 5589 6012 6228 8761 11022
Size: 1848 659 1745 1337 423 216 2533 2261
Fragments arranged by size:
2533 2261 1848 1745 1337 659 423 216
BstYI r'GATC_y
Cuts at: 0 227 476 887 2007 2088 2142 3449 5439
Size: 227 249 411 1120 81 54 1307 1990
Cuts at: 5439 5532 6188 6491 6809 7182 9034 10853 11022
Size: 93 656 303 318 373 1852 1819 169
Fragments arranged by size:
1990 1852 1819 1307 1120 656 411 373
318 303 249 227 169 93 81 54
Bsu36I CC'TnA_GG
Cuts at: 0 373 2491 4273 4764 4984 5188 5206 5726
Size: 373 2118 1782 491 220 204 18 520
Cuts at: 5726 6718 6736 6986 7321 8632 9493 11022
Size: 992 18 250 335 1311 861 1529
Fragments arranged by size:
2118 1782 1529 1311 992 861 520 491
373 335 250 220 204 18 18
BtgI C'CryG_G
Cuts at: 0 104 1752 2684 3500 4439 4446 4623 5042
Size: 104 1648 932 816 939 7 177 419
Cuts at: 5042 5417 7047 7314 7829 7910 7917 8939 10604
Size: 375 1630 267 515 81 7 1022 1665
Cuts at: 10604 10683 10806 11022
Size: 79 123 216
Fragments arranged by size:
1665 1648 1630 1022 939 932 816 515
419 375 267 216 177 123 104 81
79 7 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 635 8011 11022
Size: 635 7376 3011
Fragments arranged by size:
7376 3011 635
BtsI GCAGTG_nn'
Cuts at: 0 700 2819 3235 3610 3659 3988 4804 5140
Size: 700 2119 416 375 49 329 816 336
Cuts at: 5140 5783 5934 6864 6961 8510 8533 8571 10913
Size: 643 151 930 97 1549 23 38 2342
Cuts at: 10913 11022
Size: 109
Fragments arranged by size:
2342 2119 1549 930 816 700 643 416
375 336 329 151 109 97 49 38
23
DraIII CAC_nnn'GTG
Cuts at: 0 185 2995 2997 4791 5337 8907 10353 11022
Size: 185 2810 2 1794 546 3570 1446 669
Fragments arranged by size:
3570 2810 1794 1446 669 546 185 2
DrdI GACnn_nn'nnGTC
Cuts at: 0 5454 11022
Size: 5454 5568
EaeI y'GGCC_r
Cuts at: 0 210 1376 1664 2333 2393 3639 4155 4454
Size: 210 1166 288 669 60 1246 516 299
Cuts at: 4454 4466 4526 4936 5156 5360 6174 6452 6911
Size: 12 60 410 220 204 814 278 459
Cuts at: 6911 8339 8744 8763 8909 9374 9866 11022
Size: 1428 405 19 146 465 492 1156
Fragments arranged by size:
1428 1246 1166 1156 814 669 516 492
465 459 410 405 299 288 278 220
210 204 146 60 60 19 12
EagI C'GGCC_G
Cuts at: 0 2333 2393 4936 6174 6911 11022
Size: 2333 60 2543 1238 737 4111
Fragments arranged by size:
4111 2543 2333 1238 737 60
EarI CTCTTCn'nnn_
Cuts at: 0 1114 2959 3986 4472 6059 6163 6239 7523
Size: 1114 1845 1027 486 1587 104 76 1284
Cuts at: 7523 8005 8588 9425 10064 10412 10871 11022
Size: 482 583 837 639 348 459 151
Fragments arranged by size:
1845 1587 1284 1114 1027 837 639 583
486 482 459 348 151 104 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2278 4764 5395 5983 6427 6777 8197 8394
Size: 2278 2486 631 588 444 350 1420 197
Cuts at: 8394 9295 11022
Size: 901 1727
Fragments arranged by size:
2486 2278 1727 1420 901 631 588 444
350 197
EcoICRI GAG'CTC
Cuts at: 0 30 285 361 2721 3089 4242 4331 4596
Size: 30 255 76 2360 368 1153 89 265
Cuts at: 4596 4692 5039 6236 7748 8040 8472 9021 9669
Size: 96 347 1197 1512 292 432 549 648
Cuts at: 9669 10461 11022
Size: 792 561
Fragments arranged by size:
2360 1512 1197 1153 792 648 561 549
432 368 347 292 265 255 96 89
76 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 364 636 880 886 1006 1228 1435
Size: 301 63 272 244 6 120 222 207
Cuts at: 1435 1540 1666 1753 1924 1984 2173 2323 2389
Size: 105 126 87 171 60 189 150 66
Cuts at: 2389 2431 2462 2529 2587 2707 2755 2806 2974
Size: 42 31 67 58 120 48 51 168
Cuts at: 2974 2983 3046 3088 3163 3271 3463 3598 3643
Size: 9 63 42 75 108 192 135 45
Cuts at: 3643 3880 3925 3931 4228 4324 4341 4411 4525
Size: 237 45 6 297 96 17 70 114
Cuts at: 4525 4678 4852 4933 5055 5197 5632 5662 5707
Size: 153 174 81 122 142 435 30 45
Cuts at: 5707 5836 5959 5995 6202 6304 6355 6535 6574
Size: 129 123 36 207 102 51 180 39
Cuts at: 6574 6608 6772 6793 6880 7207 7246 7408 7611
Size: 34 164 21 87 327 39 162 203
Cuts at: 7611 7747 7882 7894 7924 7986 8053 8305 8332
Size: 136 135 12 30 62 67 252 27
Cuts at: 8332 8416 8428 8515 8599 8679 8686 8833 8950
Size: 84 12 87 84 80 7 147 117
Cuts at: 8950 9046 9113 9142 9214 9349 9430 9535 9655
Size: 96 67 29 72 135 81 105 120
Cuts at: 9655 9790 10234 10306 10351 10504 10647 11022
Size: 135 444 72 45 153 143 375
Fragments arranged by size:
444 435 375 327 301 297 272 252
244 237 222 207 207 203 192 189
180 174 171 168 164 162 153 153
150 147 143 142 136 135 135 135
135 129 126 123 122 120 120 120
117 114 108 105 105 102 96 96
87 87 87 84 84 81 81 80
75 72 72 70 67 67 67 66
63 63 62 60 58 51 51 48
45 45 45 45 42 42 39 39
36 34 31 30 30 29 27 21
17 12 12 9 7 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 372 853 1432 1480 1615 1732 3218 3442
Size: 372 481 579 48 135 117 1486 224
Cuts at: 3442 3460 3709 4117 5209 5278 5551 6721 6799
Size: 18 249 408 1092 69 273 1170 78
Cuts at: 6799 6850 6939 7159 7927 8559 9472 9703 9868
Size: 51 89 220 768 632 913 231 165
Cuts at: 9868 10120 11022
Size: 252 902
Fragments arranged by size:
1486 1170 1092 913 902 768 632 579
481 408 372 273 252 249 231 224
220 165 135 117 89 78 69 51
48 18
EcoO109I rG'GnC_Cy
Cuts at: 0 81 300 1101 1148 1167 1236 1452 1886
Size: 81 219 801 47 19 69 216 434
Cuts at: 1886 1896 2046 2126 2503 2504 2646 2666 2841
Size: 10 150 80 377 1 142 20 175
Cuts at: 2841 2958 3072 3552 3726 3781 4110 4148 4319
Size: 117 114 480 174 55 329 38 171
Cuts at: 4319 4320 4347 4536 5183 5220 5283 5598 5722
Size: 1 27 189 647 37 63 315 124
Cuts at: 5722 5807 5892 6537 6693 6714 6855 6981 7085
Size: 85 85 645 156 21 141 126 104
Cuts at: 7085 7164 7373 7688 7722 7815 7869 8015 8364
Size: 79 209 315 34 93 54 146 349
Cuts at: 8364 8451 9105 9405 9861 10113 10227 10260 10529
Size: 87 654 300 456 252 114 33 269
Cuts at: 10529 10575 10688 11022
Size: 46 113 334
Fragments arranged by size:
801 654 647 645 480 456 434 377
349 334 329 315 315 300 269 252
219 216 209 189 175 174 171 156
150 146 142 141 126 124 117 114
114 113 104 93 87 85 85 81
80 79 69 63 55 54 47 46
38 37 34 33 27 21 20 19
10 1 1
EcoRV GAT'ATC
Cuts at: 0 2890 11022
Size: 2890 8132
FspI TGC'GCA
Cuts at: 0 1936 3376 4013 11022
Size: 1936 1440 637 7009
Fragments arranged by size:
7009 1936 1440 637
HaeII r_GCGC'y
Cuts at: 0 804 1290 1446 2030 2120 2358 3291 6351
Size: 804 486 156 584 90 238 933 3060
Cuts at: 6351 6390 7396 7457 7999 9537 9768 9966 11022
Size: 39 1006 61 542 1538 231 198 1056
Fragments arranged by size:
3060 1538 1056 1006 933 804 584 542
486 238 231 198 156 90 61 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 664 696 787 819 2096 2128 2278 2296
Size: 664 32 91 32 1277 32 150 18
Cuts at: 2296 2310 2328 3388 3420 4339 4371 5851 5883
Size: 14 18 1060 32 919 32 1480 32
Cuts at: 5883 6872 6904 7884 7916 9019 9051 9994 10026
Size: 989 32 980 32 1103 32 943 32
Cuts at: 10026 10991 11022
Size: 965 31
Fragments arranged by size:
1480 1277 1103 1060 989 980 965 943
919 664 150 91 32 32 32 32
32 32 32 32 32 32 31 18
18 14
HindIII A'AGCT_T
Cuts at: 0 4205 10270 11022
Size: 4205 6065 752
Fragments arranged by size:
6065 4205 752
KasI G'GCGC_C
Cuts at: 0 2026 2354 3287 6347 9764 11022
Size: 2026 328 933 3060 3417 1258
Fragments arranged by size:
3417 3060 2026 1258 933 328
KpnI G_GTAC'C
Cuts at: 0 7068 10598 11022
Size: 7068 3530 424
Fragments arranged by size:
7068 3530 424
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 1884 2654 2937 5438 6903 7658 9569
Size: 650 1234 770 283 2501 1465 755 1911
Cuts at: 9569 11022
Size: 1453
Fragments arranged by size:
2501 1911 1465 1453 1234 770 755 650
283
MscI TGG'CCA
Cuts at: 0 1378 4157 4528 6454 8341 8765 9868 11022
Size: 1378 2779 371 1926 1887 424 1103 1154
Fragments arranged by size:
2779 1926 1887 1378 1154 1103 424 371
MslI CAynn'nnrTG
Cuts at: 0 160 586 1816 5851 7931 8140 11022
Size: 160 426 1230 4035 2080 209 2882
Fragments arranged by size:
4035 2882 2080 1230 426 209 160
MspA1I CmG'CkG
Cuts at: 0 564 759 939 984 1260 1266 1499 1644
Size: 564 195 180 45 276 6 233 145
Cuts at: 1644 1692 1920 1980 2184 2566 2952 2985 3045
Size: 48 228 60 204 382 386 33 60
Cuts at: 3045 3066 3117 3372 3414 3480 3841 3864 4072
Size: 21 51 255 42 66 361 23 208
Cuts at: 4072 4281 4302 4389 4545 4941 5133 5355 5622
Size: 209 21 87 156 396 192 222 267
Cuts at: 5622 5673 5814 5937 6012 6090 6513 6684 7011
Size: 51 141 123 75 78 423 171 327
Cuts at: 7011 7615 7713 7737 7919 8049 8223 8749 8928
Size: 604 98 24 182 130 174 526 179
Cuts at: 8928 9237 9312 9327 9664 10137 10560 10679 10685
Size: 309 75 15 337 473 423 119 6
Cuts at: 10685 10738 11022
Size: 53 284
Fragments arranged by size:
604 564 526 473 423 423 396 386
382 361 337 327 309 284 276 267
255 233 228 222 209 208 204 195
192 182 180 179 174 171 156 145
141 130 123 119 98 87 78 75
75 66 60 60 53 51 51 48
45 42 33 24 23 21 21 15
6 6
NaeI GCC'GGC
Cuts at: 0 1664 5371 11022
Size: 1664 3707 5651
Fragments arranged by size:
5651 3707 1664
NarI GG'CG_CC
Cuts at: 0 2027 2355 3288 6348 9765 11022
Size: 2027 328 933 3060 3417 1257
Fragments arranged by size:
3417 3060 2027 1257 933 328
NcoI C'CATG_G
Cuts at: 0 104 2684 3500 4439 4446 4623 5042 5417
Size: 104 2580 816 939 7 177 419 375
Cuts at: 5417 7047 7829 8939 10604 11022
Size: 1630 782 1110 1665 418
Fragments arranged by size:
2580 1665 1630 1110 939 816 782 419
418 375 177 104 7
NgoMIV G'CCGG_C
Cuts at: 0 1662 5369 11022
Size: 1662 3707 5653
Fragments arranged by size:
5653 3707 1662
NheI G'CTAG_C
Cuts at: 0 9228 9978 11022
Size: 9228 750 1044
Fragments arranged by size:
9228 1044 750
NotI GC'GGCC_GC
Cuts at: 0 2333 4936 6174 11022
Size: 2333 2603 1238 4848
Fragments arranged by size:
4848 2603 2333 1238
NspI r_CATG'y
Cuts at: 0 159 1413 1596 4620 4844 6439 8202 11022
Size: 159 1254 183 3024 224 1595 1763 2820
Fragments arranged by size:
3024 2820 1763 1595 1254 224 183 159
PasI CC'CwG_GG
Cuts at: 0 1806 1998 2500 2536 3777 7959 10905 11022
Size: 1806 192 502 36 1241 4182 2946 117
Fragments arranged by size:
4182 2946 1806 1241 502 192 117 36
PflMI CCAn_nnn'nTGG
Cuts at: 0 1404 1455 1644 3171 3501 4389 4452 4809
Size: 1404 51 189 1527 330 888 63 357
Cuts at: 4809 5033 5358 5640 6750 6858 7167 7650 8031
Size: 224 325 282 1110 108 309 483 381
Cuts at: 8031 8079 8589 8811 8907 9063 9927 10670 11022
Size: 48 510 222 96 156 864 743 352
Fragments arranged by size:
1527 1404 1110 888 864 743 510 483
381 357 352 330 325 309 282 224
222 189 156 108 96 63 51 48
PfoI T'CCnGG_A
Cuts at: 0 341 902 1310 2478 3947 4472 5123 5558
Size: 341 561 408 1168 1469 525 651 435
Cuts at: 5558 6059 6770 6812 7561 8273 9023 9062 9191
Size: 501 711 42 749 712 750 39 129
Cuts at: 9191 10718 11022
Size: 1527 304
Fragments arranged by size:
1527 1469 1168 750 749 712 711 651
561 525 501 435 408 341 304 129
42 39
PmlI CAC'GTG
Cuts at: 0 2361 4104 7101 11022
Size: 2361 1743 2997 3921
Fragments arranged by size:
3921 2997 2361 1743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 10445 10477 11022
Size: 10445 32 545
Fragments arranged by size:
10445 545 32
PpuMI rG'GwC_Cy
Cuts at: 0 81 3552 4347 5183 5220 5283 5598 5892
Size: 81 3471 795 836 37 63 315 294
Cuts at: 5892 6537 6714 6855 6981 7815 7869 8015 9405
Size: 645 177 141 126 834 54 146 1390
Cuts at: 9405 10113 10575 10688 11022
Size: 708 462 113 334
Fragments arranged by size:
3471 1390 836 834 795 708 645 462
334 315 294 177 146 141 126 113
81 63 54 37
PshAI GACnn'nnGTC
Cuts at: 0 817 11022
Size: 817 10205
PspOMI G'GGCC_C
Cuts at: 0 1886 1895 2503 2645 2666 3281 4319 4853
Size: 1886 9 608 142 21 615 1038 534
Cuts at: 4853 5807 6503 7373 10226 11022
Size: 954 696 870 2853 796
Fragments arranged by size:
2853 1886 1038 954 870 796 696 615
608 534 142 21 9
PspXI vC'TCGA_Gb
Cuts at: 0 6662 11022
Size: 6662 4360
PstI C_TGCA'G
Cuts at: 0 45 923 1244 1259 1265 1619 1658 1718
Size: 45 878 321 15 6 354 39 60
Cuts at: 1718 1832 1919 2036 2225 2246 2707 3020 3080
Size: 114 87 117 189 21 461 313 60
Cuts at: 3080 3116 3242 3476 3632 3743 3869 4049 4118
Size: 36 126 234 156 111 126 180 69
Cuts at: 4118 4286 4373 4517 4544 4550 4868 5147 5228
Size: 168 87 144 27 6 318 279 81
Cuts at: 5228 5447 5678 5697 5786 5900 6039 6083 6137
Size: 219 231 19 89 114 139 44 54
Cuts at: 6137 6386 6692 6785 6875 6968 7439 7718 8054
Size: 249 306 93 90 93 471 279 336
Cuts at: 8054 8066 8222 8246 8648 8795 8987 9176 9242
Size: 12 156 24 402 147 192 189 66
Cuts at: 9242 9305 9317 9630 9752 9852 9899 10187 10208
Size: 63 12 313 122 100 47 288 21
Cuts at: 10208 10268 10472 10920 11022
Size: 60 204 448 102
Fragments arranged by size:
878 471 461 448 402 354 336 321
318 313 313 306 288 279 279 249
234 231 219 204 192 189 189 180
168 156 156 147 144 139 126 126
122 117 114 114 111 102 100 93
93 90 89 87 87 81 69 66
63 60 60 60 54 47 45 44
39 36 27 24 21 21 19 15
12 12 6 6
PvuII CAG'CTG
Cuts at: 0 759 939 984 1260 1499 1644 1692 2184
Size: 759 180 45 276 239 145 48 492
Cuts at: 2184 2566 2952 2985 3045 3066 3117 3372 3480
Size: 382 386 33 60 21 51 255 108
Cuts at: 3480 3841 3864 4072 4281 4302 4389 4545 5133
Size: 361 23 208 209 21 87 156 588
Cuts at: 5133 5622 5673 5814 6012 6513 7713 7737 8049
Size: 489 51 141 198 501 1200 24 312
Cuts at: 8049 8928 9237 9312 9327 9664 10137 10560 10679
Size: 879 309 75 15 337 473 423 119
Cuts at: 10679 11022
Size: 343
Fragments arranged by size:
1200 879 759 588 501 492 489 473
423 386 382 361 343 337 312 309
276 255 239 209 208 198 180 156
145 141 119 108 87 75 60 51
51 48 45 33 24 23 21 21
15
SacI G_AGCT'C
Cuts at: 0 32 287 363 2723 3091 4244 4333 4598
Size: 32 255 76 2360 368 1153 89 265
Cuts at: 4598 4694 5041 6238 7750 8042 8474 9023 9671
Size: 96 347 1197 1512 292 432 549 648
Cuts at: 9671 10463 11022
Size: 792 559
Fragments arranged by size:
2360 1512 1197 1153 792 648 559 549
432 368 347 292 265 255 96 89
76 32
SacII CC_GC'GG
Cuts at: 0 7920 10686 11022
Size: 7920 2766 336
Fragments arranged by size:
7920 2766 336
SanDI GG'GwC_CC
Cuts at: 0 81 5183 6981 7815 8015 10575 11022
Size: 81 5102 1798 834 200 2560 447
Fragments arranged by size:
5102 2560 1798 834 447 200 81
SapI GCTCTTCn'nnn_
Cuts at: 0 1114 6059 6163 7523 8005 8588 10064 10871
Size: 1114 4945 104 1360 482 583 1476 807
Cuts at: 10871 11022
Size: 151
Fragments arranged by size:
4945 1476 1360 1114 807 583 482 151
104
SbfI CC_TGCA'GG
Cuts at: 0 5228 5900 6083 8066 8648 8795 9305 9899
Size: 5228 672 183 1983 582 147 510 594
Cuts at: 9899 10187 11022
Size: 288 835
Fragments arranged by size:
5228 1983 835 672 594 582 510 288
183 147
ScaI AGT'ACT
Cuts at: 0 2248 4891 11022
Size: 2248 2643 6131
Fragments arranged by size:
6131 2643 2248
SexAI A'CCwGG_T
Cuts at: 0 6095 8105 11022
Size: 6095 2010 2917
Fragments arranged by size:
6095 2917 2010
SfcI C'TryA_G
Cuts at: 0 41 142 919 1078 1117 1240 1255 1261
Size: 41 101 777 159 39 123 15 6
Cuts at: 1261 1564 1615 1654 1714 1828 1915 2032 2221
Size: 303 51 39 60 114 87 117 189
Cuts at: 2221 2242 2703 3016 3076 3112 3238 3472 3628
Size: 21 461 313 60 36 126 234 156
Cuts at: 3628 3739 3865 4045 4114 4282 4324 4369 4513
Size: 111 126 180 69 168 42 45 144
Cuts at: 4513 4540 4546 4864 5011 5143 5224 5443 5674
Size: 27 6 318 147 132 81 219 231
Cuts at: 5674 5693 5782 5896 6035 6079 6133 6382 6688
Size: 19 89 114 139 44 54 249 306
Cuts at: 6688 6781 6871 6964 7435 7708 7714 7732 8050
Size: 93 90 93 471 273 6 18 318
Cuts at: 8050 8062 8218 8242 8644 8791 8983 9172 9238
Size: 12 156 24 402 147 192 189 66
Cuts at: 9238 9301 9313 9626 9748 9811 9848 9895 10183
Size: 63 12 313 122 63 37 47 288
Cuts at: 10183 10204 10264 10468 10916 11022
Size: 21 60 204 448 106
Fragments arranged by size:
777 471 461 448 402 318 318 313
313 306 303 288 273 249 234 231
219 204 192 189 189 180 168 159
156 156 147 147 144 139 132 126
126 123 122 117 114 114 111 106
101 93 93 90 89 87 81 69
66 63 63 60 60 60 54 51
47 45 44 42 41 39 39 37
36 27 24 21 21 19 18 15
12 12 6 6 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1099 1894 3280 8362 8668 10306 11022
Size: 1099 795 1386 5082 306 1638 716
Fragments arranged by size:
5082 1638 1386 1099 795 716 306
SfoI GGC'GCC
Cuts at: 0 2028 2356 3289 6349 9766 11022
Size: 2028 328 933 3060 3417 1256
Fragments arranged by size:
3417 3060 2028 1256 933 328
SmaI CCC'GGG
Cuts at: 0 25 2148 2197 2440 5983 6355 8848 8917
Size: 25 2123 49 243 3543 372 2493 69
Cuts at: 8917 10491 11022
Size: 1574 531
Fragments arranged by size:
3543 2493 2123 1574 531 372 243 69
49 25
SmlI C'TyrA_G
Cuts at: 0 1324 1739 2619 4054 6662 6970 10830 11022
Size: 1324 415 880 1435 2608 308 3860 192
Fragments arranged by size:
3860 2608 1435 1324 880 415 308 192
SphI G_CATG'C
Cuts at: 0 1413 6439 8202 11022
Size: 1413 5026 1763 2820
Fragments arranged by size:
5026 2820 1763 1413
SrfI GCCC'GGGC
Cuts at: 0 2440 11022
Size: 2440 8582
StuI AGG'CCT
Cuts at: 0 3685 4187 4552 6577 6805 8056 8356 8554
Size: 3685 502 365 2025 228 1251 300 198
Cuts at: 8554 9883 10300 11022
Size: 1329 417 722
Fragments arranged by size:
3685 2025 1329 1251 722 502 417 365
300 228 198
StyI C'CwwG_G
Cuts at: 0 104 380 491 536 1835 2684 3500 3509
Size: 104 276 111 45 1299 849 816 9
Cuts at: 3509 3705 4439 4446 4623 5042 5417 6050 6227
Size: 196 734 7 177 419 375 633 177
Cuts at: 6227 7047 7088 7820 7829 8114 8939 9221 9368
Size: 820 41 732 9 285 825 282 147
Cuts at: 9368 9479 9869 10056 10604 10982 11022
Size: 111 390 187 548 378 40
Fragments arranged by size:
1299 849 825 820 816 734 732 633
548 419 390 378 375 285 282 276
196 187 177 177 147 111 111 104
45 41 40 9 9 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1072 3851 4911 5566 6022 6060 6478 6916
Size: 1072 2779 1060 655 456 38 418 438
Cuts at: 6916 8270 8712 9543 11022
Size: 1354 442 831 1479
Fragments arranged by size:
2779 1479 1354 1072 1060 831 655 456
442 438 418 38
TatI w'GTAC_w
Cuts at: 0 239 2246 3258 4889 11022
Size: 239 2007 1012 1631 6133
Fragments arranged by size:
6133 2007 1631 1012 239
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 826 858 1748 1780 1967 1999 4137 4169
Size: 826 32 890 32 187 32 2138 32
Cuts at: 4169 5977 6009 6936 6968 7090 7122 10810 10842
Size: 1808 32 927 32 122 32 3688 32
Cuts at: 10842 11022
Size: 180
Fragments arranged by size:
3688 2138 1808 927 890 826 187 180
122 32 32 32 32 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 7123 11022
Size: 7123 3899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2533 3507 5074 7651 8455 9138 11022
Size: 2533 974 1567 2577 804 683 1884
Fragments arranged by size:
2577 2533 1884 1567 974 804 683
XhoI C'TCGA_G
Cuts at: 0 6662 11022
Size: 6662 4360
XmaI C'CCGG_G
Cuts at: 0 23 2146 2195 2438 5981 6353 8846 8915
Size: 23 2123 49 243 3543 372 2493 69
Cuts at: 8915 10489 11022
Size: 1574 533
Fragments arranged by size:
3543 2493 2123 1574 533 372 243 69
49 23
XmnI GAAnn'nnTTC
Cuts at: 0 175 6068 8692 11022
Size: 175 5893 2624 2330
Fragments arranged by size:
5893 2624 2330 175
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII Hin4I
HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NotI NspI PasI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SacII SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SmaI SmlI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AgeI AhdI AscI AseI AsiSI AvrII BclI
BfrBI BsaBI BsiWI BstBI BstZ17I ClaI CspCI DraI
EcoRI FalI FseI FspAI HincII HpaI MfeI MluI
NdeI NruI NsiI PacI PciI PmeI PsiI PsrI
PvuI RsrII SalI SgfI SgrAI SnaBI SpeI SspI
SwaI XbaI ZraI