Restriction map for
pFN21AE2688
(Linear) (Six-base) MAPSORT of: KIEE2688.seq Check: 1437 from: 1 to: 4182
KIEE2688.orf
With 182 enzymes: SgfI *
May 11, 2017 11:17 ..
AatII G_ACGT'C
Cuts at: 0 1347 4182
Size: 1347 2835
AccI GT'mk_AC
Cuts at: 0 2166 4182
Size: 2166 2016
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 918 1626 2332 2752 3599 4108 4182
Size: 442 476 708 706 420 847 509 74
Fragments arranged by size:
847 708 706 509 476 442 420 74
AflIII A'CryG_T
Cuts at: 0 985 4182
Size: 985 3197
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3086 3118 4182
Size: 3086 32 1064
Fragments arranged by size:
3086 1064 32
AlwNI CAG_nnn'CTG
Cuts at: 0 867 3097 4182
Size: 867 2230 1085
Fragments arranged by size:
2230 1085 867
ApaI G_GGCC'C
Cuts at: 0 140 4182
Size: 140 4042
ApoI r'AATT_y
Cuts at: 0 430 532 968 1067 1850 1947 2154 2206
Size: 430 102 436 99 783 97 207 52
Cuts at: 2206 3069 3879 4138 4182
Size: 863 810 259 44
Fragments arranged by size:
863 810 783 436 430 259 207 102
99 97 52 44
AvaI C'yCGr_G
Cuts at: 0 90 1721 4182
Size: 90 1631 2461
Fragments arranged by size:
2461 1631 90
AvrII C'CTAG_G
Cuts at: 0 351 4182
Size: 351 3831
BanI G'GyrC_C
Cuts at: 0 643 4182
Size: 643 3539
BanII G_rGCy'C
Cuts at: 0 140 163 258 2754 3816 4182
Size: 140 23 95 2496 1062 366
Fragments arranged by size:
2496 1062 366 140 95 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 3512 4182
Size: 3512 670
BciVI GTATCCnnnnn_n'
Cuts at: 0 959 4182
Size: 959 3223
BclI T'GATC_A
Cuts at: 0 1416 4182
Size: 1416 2766
BfrBI ATG'CAT
Cuts at: 0 826 4182
Size: 826 3356
BglI GCCn_nnn'nGGC
Cuts at: 0 46 4182
Size: 46 4136
BglII A'GATC_T
Cuts at: 0 270 4182
Size: 270 3912
Bme1580I G_kGCm'C
Cuts at: 0 140 711 4182
Size: 140 571 3471
Fragments arranged by size:
3471 571 140
BmrI ACTGGGnnnn_n'
Cuts at: 0 679 4182
Size: 679 3503
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 1852 2723 4118 4182
Size: 889 963 871 1395 64
Fragments arranged by size:
1395 963 889 871 64
Bpu10I CC'TnA_GC
Cuts at: 0 342 4182
Size: 342 3840
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1508 2545 4182
Size: 1508 1037 1637
Fragments arranged by size:
1637 1508 1037
BsaI GGTCTCn'nnnn_
Cuts at: 0 2855 3274 4182
Size: 2855 419 908
Fragments arranged by size:
2855 908 419
BsaAI yAC'GTr
Cuts at: 0 382 4182
Size: 382 3800
BsaBI GATnn'nnATC
Cuts at: 0 3712 4182
Size: 3712 470
BsaHI Gr'CG_yC
Cuts at: 0 1344 4182
Size: 1344 2838
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4091 4121 4182
Size: 4091 30 61
Fragments arranged by size:
4091 61 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 223 1816 3694 4182
Size: 67 156 1593 1878 488
Fragments arranged by size:
1878 1593 488 156 67
BseYI C'CCAG_C
Cuts at: 0 162 943 4182
Size: 162 781 3239
Fragments arranged by size:
3239 781 162
BsiHKAI G_wGCw'C
Cuts at: 0 2754 3816 4182
Size: 2754 1062 366
Fragments arranged by size:
2754 1062 366
BsmI GAATG_Cn'
Cuts at: 0 296 753 828 1397 1874 3174 3271 4182
Size: 296 457 75 569 477 1300 97 911
Fragments arranged by size:
1300 911 569 477 457 296 97 75
Bsp1286I G_dGCh'C
Cuts at: 0 140 163 258 711 2754 3816 4182
Size: 140 23 95 453 2043 1062 366
Fragments arranged by size:
2043 1062 453 366 140 95 23
BspHI T'CATG_A
Cuts at: 0 2574 3971 4182
Size: 2574 1397 211
Fragments arranged by size:
2574 1397 211
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 343 4182
Size: 343 3839
BsrBI CCG'CTC
Cuts at: 0 63 127 341 4182
Size: 63 64 214 3841
Fragments arranged by size:
3841 214 64 63
BsrDI GCAATG_nn'
Cuts at: 0 179 1019 1330 1594 2636 3263 3545 4182
Size: 179 840 311 264 1042 627 282 637
Fragments arranged by size:
1042 840 637 627 311 282 264 179
BsrFI r'CCGG_y
Cuts at: 0 95 2242 4182
Size: 95 2147 1940
Fragments arranged by size:
2147 1940 95
BsrGI T'GTAC_A
Cuts at: 0 1691 2490 4182
Size: 1691 799 1692
Fragments arranged by size:
1692 1691 799
BssSI C'ACGA_G
Cuts at: 0 1472 2963 4182
Size: 1472 1491 1219
Fragments arranged by size:
1491 1472 1219
BstBI TT'CG_AA
Cuts at: 0 282 4182
Size: 282 3900
BstEII G'GTnAC_C
Cuts at: 0 3007 4182
Size: 3007 1175
BstYI r'GATC_y
Cuts at: 0 16 270 678 4182
Size: 16 254 408 3504
Fragments arranged by size:
3504 408 254 16
Bsu36I CC'TnA_GG
Cuts at: 0 3874 4182
Size: 3874 308
BtgI C'CryG_G
Cuts at: 0 140 4182
Size: 140 4042
BtsI GCAGTG_nn'
Cuts at: 0 3428 4182
Size: 3428 754
DraIII CAC_nnn'GTG
Cuts at: 0 1140 4182
Size: 1140 3042
EaeI y'GGCC_r
Cuts at: 0 143 291 1327 3446 3731 4182
Size: 143 148 1036 2119 285 451
Fragments arranged by size:
2119 1036 451 285 148 143
EarI CTCTTCn'nnn_
Cuts at: 0 413 2069 2686 4182
Size: 413 1656 617 1496
Fragments arranged by size:
1656 1496 617 413
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3736 4182
Size: 3736 446
EcoICRI GAG'CTC
Cuts at: 0 2752 3814 4182
Size: 2752 1062 368
Fragments arranged by size:
2752 1062 368
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 889 918 1626 1852 2332 2723 2752
Size: 442 447 29 708 226 480 391 29
Cuts at: 2752 3599 4108 4118 4182
Size: 847 509 10 64
Fragments arranged by size:
847 708 509 480 447 442 391 226
64 29 29 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3001 4182
Size: 3001 1181
EcoRI G'AATT_C
Cuts at: 0 3069 3879 4182
Size: 3069 810 303
Fragments arranged by size:
3069 810 303
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 532 4182
Size: 532 3650
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 225 255 257 287 2736 2768 3988 4020
Size: 225 30 2 30 2449 32 1220 32
Cuts at: 4020 4091 4123 4182
Size: 71 32 59
Fragments arranged by size:
2449 1220 225 71 59 32 32 32
30 30 2
HincII GTy'rAC
Cuts at: 0 2167 4182
Size: 2167 2015
HindIII A'AGCT_T
Cuts at: 0 307 412 4131 4182
Size: 307 105 3719 51
Fragments arranged by size:
3719 307 105 51
MfeI C'AATT_G
Cuts at: 0 3836 4182
Size: 3836 346
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1320 1327 2144 3668 4182
Size: 1320 7 817 1524 514
Fragments arranged by size:
1524 1320 817 514 7
MscI TGG'CCA
Cuts at: 0 1329 3448 3733 4182
Size: 1329 2119 285 449
Fragments arranged by size:
2119 1329 449 285
MslI CAynn'nnrTG
Cuts at: 0 494 769 2059 4182
Size: 494 275 1290 2123
Fragments arranged by size:
2123 1290 494 275
MspA1I CmG'CkG
Cuts at: 0 1830 4182
Size: 1830 2352
NaeI GCC'GGC
Cuts at: 0 97 4182
Size: 97 4085
NdeI CA'TA_TG
Cuts at: 0 828 3153 4182
Size: 828 2325 1029
Fragments arranged by size:
2325 1029 828
NgoMIV G'CCGG_C
Cuts at: 0 95 4182
Size: 95 4087
NsiI A_TGCA'T
Cuts at: 0 828 4182
Size: 828 3354
NspI r_CATG'y
Cuts at: 0 106 727 989 1082 2376 4182
Size: 106 621 262 93 1294 1806
Fragments arranged by size:
1806 1294 621 262 106 93
PciI A'CATG_T
Cuts at: 0 985 4182
Size: 985 3197
PflMI CCAn_nnn'nTGG
Cuts at: 0 1830 3444 4182
Size: 1830 1614 738
Fragments arranged by size:
1830 1614 738
PfoI T'CCnGG_A
Cuts at: 0 1886 4182
Size: 1886 2296
PshAI GACnn'nnGTC
Cuts at: 0 3430 4182
Size: 3430 752
PsiI TTA'TAA
Cuts at: 0 1757 3752 4182
Size: 1757 1995 430
Fragments arranged by size:
1995 1757 430
PspOMI G'GGCC_C
Cuts at: 0 136 4182
Size: 136 4046
PspXI vC'TCGA_Gb
Cuts at: 0 1721 4182
Size: 1721 2461
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 375 407 4182
Size: 375 32 3775
Fragments arranged by size:
3775 375 32
PstI C_TGCA'G
Cuts at: 0 1223 1613 4182
Size: 1223 390 2569
Fragments arranged by size:
2569 1223 390
PvuII CAG'CTG
Cuts at: 0 1830 4182
Size: 1830 2352
SacI G_AGCT'C
Cuts at: 0 2754 3816 4182
Size: 2754 1062 366
Fragments arranged by size:
2754 1062 366
SalI G'TCGA_C
Cuts at: 0 2165 4182
Size: 2165 2017
SfcI C'TryA_G
Cuts at: 0 1219 1609 1866 3207 3820 4182
Size: 1219 390 257 1341 613 362
Fragments arranged by size:
1341 1219 613 390 362 257
SmaI CCC'GGG
Cuts at: 0 92 4182
Size: 92 4090
SmlI C'TyrA_G
Cuts at: 0 1523 1721 2524 4182
Size: 1523 198 803 1658
Fragments arranged by size:
1658 1523 803 198
SnaBI TAC'GTA
Cuts at: 0 382 4182
Size: 382 3800
SphI G_CATG'C
Cuts at: 0 106 727 1082 4182
Size: 106 621 355 3100
Fragments arranged by size:
3100 621 355 106
SrfI GCCC'GGGC
Cuts at: 0 92 4182
Size: 92 4090
StuI AGG'CCT
Cuts at: 0 2258 4182
Size: 2258 1924
StyI C'CwwG_G
Cuts at: 0 351 1796 2566 4182
Size: 351 1445 770 1616
Fragments arranged by size:
1616 1445 770 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1743 4182
Size: 1743 2439
TatI w'GTAC_w
Cuts at: 0 1407 1691 2490 2870 4182
Size: 1407 284 799 380 1312
Fragments arranged by size:
1407 1312 799 380 284
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2377 4182
Size: 2377 1805
XhoI C'TCGA_G
Cuts at: 0 1721 4182
Size: 1721 2461
XmaI C'CCGG_G
Cuts at: 0 90 4182
Size: 90 4092
XmnI GAAnn'nnTTC
Cuts at: 0 758 1301 3271 3479 3929 4182
Size: 758 543 1970 208 450 253
Fragments arranged by size:
1970 758 543 450 253 208
ZraI GAC'GTC
Cuts at: 0 1345 4182
Size: 1345 2837
Enzymes that do cut:
AatII AccI AcuI AflIII AloI AlwNI ApaI ApoI
AvaI AvrII BanI BanII BbsI BciVI BclI BfrBI
BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstBI BstEII BstYI Bsu36I BtgI BtsI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI Hin4I
HincII HindIII MfeI MmeI MscI MslI MspA1I NaeI
NdeI NgoMIV NsiI NspI PciI PflMI PfoI PshAI
PsiI PspOMI PspXI PsrI PstI PvuII SacI SalI
SfcI SmaI SmlI SnaBI SphI SrfI StuI StyI
TaqII TatI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI ApaLI AscI AseI AsiSI BaeI BamHI BbeI
BbvCI BcgI BlpI BmgBI BmtI BplI BsaWI BsgI
BsiEI BsiWI BsmBI BspEI BssHII BstAPI BstXI BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EcoO109I EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
NarI NcoI NheI NotI NruI PacI PasI PmeI
PmlI PpiI PpuMI PvuI RsrII SacII SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SpeI
SspI SwaI TstI Tth111I XbaI