Restriction map for
pFN21AE2681
(Linear) (Six-base) MAPSORT of: KIEE2681.seq Check: 8528 from: 1 to: 1098
KIEE2681.orf
With 182 enzymes: SgfI *
March 10, 2017 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 607 1098
Size: 607 491
AccI GT'mk_AC
Cuts at: 0 780 1098
Size: 780 318
Acc65I G'GTAC_C
Cuts at: 0 608 1098
Size: 608 490
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 192 814 1098
Size: 58 134 622 284
Fragments arranged by size:
622 284 134 58
AflIII A'CryG_T
Cuts at: 0 304 1098
Size: 304 794
AleI CACnn'nnGTG
Cuts at: 0 307 1098
Size: 307 791
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 250 282 1098
Size: 250 32 816
Fragments arranged by size:
816 250 32
AlwNI CAG_nnn'CTG
Cuts at: 0 174 332 1098
Size: 174 158 766
Fragments arranged by size:
766 174 158
ApaI G_GGCC'C
Cuts at: 0 391 1098
Size: 391 707
AvaI C'yCGr_G
Cuts at: 0 249 1023 1098
Size: 249 774 75
Fragments arranged by size:
774 249 75
BamHI G'GATC_C
Cuts at: 0 890 1098
Size: 890 208
BanI G'GyrC_C
Cuts at: 0 40 151 292 608 954 1098
Size: 40 111 141 316 346 144
Fragments arranged by size:
346 316 144 141 111 40
BanII G_rGCy'C
Cuts at: 0 391 487 929 1050 1098
Size: 391 96 442 121 48
Fragments arranged by size:
442 391 121 96 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 971 1098
Size: 971 127
BciVI GTATCCnnnnn_n'
Cuts at: 0 875 1098
Size: 875 223
Bme1580I G_kGCm'C
Cuts at: 0 154 391 959 1098
Size: 154 237 568 139
Fragments arranged by size:
568 237 154 139
BmrI ACTGGGnnnn_n'
Cuts at: 0 59 793 1098
Size: 59 734 305
Fragments arranged by size:
734 305 59
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 931 1098
Size: 931 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 897 923 1098
Size: 897 26 175
Fragments arranged by size:
897 175 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 157 658 1098
Size: 157 501 440
Fragments arranged by size:
501 440 157
BsaHI Gr'CG_yC
Cuts at: 0 777 1098
Size: 777 321
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 250 280 557 587 690 720 1098
Size: 250 30 277 30 103 30 378
Fragments arranged by size:
378 277 250 103 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 1098
Size: 139 959
BseYI C'CCAG_C
Cuts at: 0 417 644 1098
Size: 417 227 454
Fragments arranged by size:
454 417 227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 585 1098
Size: 585 513
BsiEI CG_ry'CG
Cuts at: 0 104 1098
Size: 104 994
BsiHKAI G_wGCw'C
Cuts at: 0 1050 1098
Size: 1050 48
BsmI GAATG_Cn'
Cuts at: 0 462 1098
Size: 462 636
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1053 1098
Size: 1053 45
Bsp1286I G_dGCh'C
Cuts at: 0 154 391 487 929 959 1050 1098
Size: 154 237 96 442 30 91 48
Fragments arranged by size:
442 237 154 96 91 48 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 485 607 1098
Size: 485 122 491
Fragments arranged by size:
491 485 122
BsrFI r'CCGG_y
Cuts at: 0 154 650 1098
Size: 154 496 448
Fragments arranged by size:
496 448 154
BstYI r'GATC_y
Cuts at: 0 53 890 1098
Size: 53 837 208
Fragments arranged by size:
837 208 53
BtgI C'CryG_G
Cuts at: 0 115 270 901 1098
Size: 115 155 631 197
Fragments arranged by size:
631 197 155 115
BtsI GCAGTG_nn'
Cuts at: 0 137 243 816 832 1098
Size: 137 106 573 16 266
Fragments arranged by size:
573 266 137 106 16
EaeI y'GGCC_r
Cuts at: 0 101 652 1098
Size: 101 551 446
Fragments arranged by size:
551 446 101
EagI C'GGCC_G
Cuts at: 0 101 1098
Size: 101 997
EcoICRI GAG'CTC
Cuts at: 0 1048 1098
Size: 1048 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 192 814 931 1098
Size: 58 134 622 117 167
Fragments arranged by size:
622 167 134 117 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 280 1098
Size: 280 818
EcoO109I rG'GnC_Cy
Cuts at: 0 388 474 1098
Size: 388 86 624
Fragments arranged by size:
624 388 86
HaeII r_GCGC'y
Cuts at: 0 140 651 1098
Size: 140 511 447
Fragments arranged by size:
511 447 140
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 622 654 690 722 882 914
Size: 343 32 247 32 36 32 160 32
Cuts at: 914 1098
Size: 184
Fragments arranged by size:
343 247 184 160 36 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 589 1098
Size: 589 509
KpnI G_GTAC'C
Cuts at: 0 612 1098
Size: 612 486
MslI CAynn'nnrTG
Cuts at: 0 307 428 920 1098
Size: 307 121 492 178
Fragments arranged by size:
492 307 178 121
MspA1I CmG'CkG
Cuts at: 0 117 750 1006 1098
Size: 117 633 256 92
Fragments arranged by size:
633 256 117 92
NaeI GCC'GGC
Cuts at: 0 652 1098
Size: 652 446
NgoMIV G'CCGG_C
Cuts at: 0 650 1098
Size: 650 448
NspI r_CATG'y
Cuts at: 0 267 308 1098
Size: 267 41 790
Fragments arranged by size:
790 267 41
PasI CC'CwG_GG
Cuts at: 0 391 1098
Size: 391 707
PciI A'CATG_T
Cuts at: 0 304 1098
Size: 304 794
PflMI CCAn_nnn'nTGG
Cuts at: 0 174 1098
Size: 174 924
PfoI T'CCnGG_A
Cuts at: 0 371 708 1098
Size: 371 337 390
Fragments arranged by size:
390 371 337
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 250 282 1098
Size: 250 32 816
Fragments arranged by size:
816 250 32
PpuMI rG'GwC_Cy
Cuts at: 0 474 1098
Size: 474 624
PspOMI G'GGCC_C
Cuts at: 0 387 1098
Size: 387 711
PstI C_TGCA'G
Cuts at: 0 482 1098
Size: 482 616
PvuII CAG'CTG
Cuts at: 0 750 1006 1098
Size: 750 256 92
Fragments arranged by size:
750 256 92
SacI G_AGCT'C
Cuts at: 0 1050 1098
Size: 1050 48
SacII CC_GC'GG
Cuts at: 0 118 1098
Size: 118 980
SbfI CC_TGCA'GG
Cuts at: 0 482 1098
Size: 482 616
SexAI A'CCwGG_T
Cuts at: 0 614 1098
Size: 614 484
SfcI C'TryA_G
Cuts at: 0 478 1098
Size: 478 620
SgrAI Cr'CCGG_yG
Cuts at: 0 154 1098
Size: 154 944
SmaI CCC'GGG
Cuts at: 0 251 1098
Size: 251 847
SmlI C'TyrA_G
Cuts at: 0 876 938 1098
Size: 876 62 160
Fragments arranged by size:
876 160 62
SrfI GCCC'GGGC
Cuts at: 0 251 1098
Size: 251 847
StyI C'CwwG_G
Cuts at: 0 995 1041 1098
Size: 995 46 57
Fragments arranged by size:
995 57 46
Tth111I GACn'n_nGTC
Cuts at: 0 871 1098
Size: 871 227
XmaI C'CCGG_G
Cuts at: 0 249 1098
Size: 249 849
XmnI GAAnn'nnTTC
Cuts at: 0 205 1098
Size: 205 893
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AloI AlwNI
ApaI AvaI BamHI BanI BanII BbsI BciVI Bme1580I
BmrI BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI
BstYI BtgI BtsI EaeI EagI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HindIII KpnI MslI MspA1I NaeI
NgoMIV NspI PasI PciI PflMI PfoI PpiI PpuMI
PspOMI PstI PvuII SacI SacII SbfI SexAI SfcI
SgrAI SmaI SmlI SrfI StyI Tth111I XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AlfI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI
BcgI BclI BfrBI BglI BglII BlpI BmgBI BmtI
BplI Bpu10I BsaAI BsaBI BsaWI BsiWI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EarI EciI EcoRI EcoRV FalI FseI FspI
FspAI HincII HpaI KasI MfeI MluI MmeI MscI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SanDI SapI ScaI SfiI SfoI SgfI SnaBI
SpeI SphI SspI StuI SwaI TaqII TatI TstI
XbaI XcmI XhoI ZraI