(Linear) (Six-base) MAPSORT of: KIEE2679.seq  Check: 4641  from: 1  to: 270



KIEE2679.orf

 With 182 enzymes: SgfI * 

                        February 17, 2017 10:47  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     270 
   Size:        196      74
 
ApoI r'AATT_y

Cuts at:      0     142     270 
   Size:        142     128
 
BsaWI w'CCGG_w

Cuts at:      0     156     270 
   Size:        156     114
 
BseYI C'CCAG_C

Cuts at:      0      72     270 
   Size:         72     198
 
BspEI T'CCGG_A

Cuts at:      0     156     270 
   Size:        156     114
 
BsrDI GCAATG_nn'

Cuts at:      0     227     270 
   Size:        227      43
 
EarI CTCTTCn'nnn_

Cuts at:      0     181     270 
   Size:        181      89
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     270 
   Size:        196      74
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     190     222     270 
   Size:        190      32      48

  Fragments arranged by size: 

                 190      48      32
 
SspI AAT'ATT

Cuts at:      0     251     270 
   Size:        251      19
 

 Enzymes that do cut:

     AcuI     ApoI    BsaWI    BseYI    BspEI    BsrDI     EarI  Eco57MI
    Hin4I     SspI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI