Restriction map for
pFN21AE2676
(Linear) (Six-base) MAPSORT of: KIEE2676.seq Check: 3936 from: 1 to: 4386
KIEE2676.orf
With 182 enzymes: SgfI *
July 27, 2017 10:26 ..
AccI GT'mk_AC
Cuts at: 0 452 1989 4386
Size: 452 1537 2397
Fragments arranged by size:
2397 1537 452
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 593 2014 2633 2803 4386
Size: 562 31 1421 619 170 1583
Fragments arranged by size:
1583 1421 619 562 170 31
AflIII A'CryG_T
Cuts at: 0 3465 3853 4386
Size: 3465 388 533
Fragments arranged by size:
3465 533 388
AgeI A'CCGG_T
Cuts at: 0 4184 4386
Size: 4184 202
AhdI GACnn_n'nnGTC
Cuts at: 0 27 4386
Size: 27 4359
AleI CACnn'nnGTG
Cuts at: 0 1748 3322 4386
Size: 1748 1574 1064
Fragments arranged by size:
1748 1574 1064
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1004 1036 2311 2343 4386
Size: 1004 32 1275 32 2043
Fragments arranged by size:
2043 1275 1004 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3824 4386
Size: 3824 562
ApaLI G'TGCA_C
Cuts at: 0 10 586 4208 4386
Size: 10 576 3622 178
Fragments arranged by size:
3622 576 178 10
ApoI r'AATT_y
Cuts at: 0 679 1246 2081 2611 3483 4386
Size: 679 567 835 530 872 903
Fragments arranged by size:
903 872 835 679 567 530
AseI AT'TA_AT
Cuts at: 0 1952 4386
Size: 1952 2434
AvaI C'yCGr_G
Cuts at: 0 56 4386
Size: 56 4330
BamHI G'GATC_C
Cuts at: 0 3423 4386
Size: 3423 963
BanI G'GyrC_C
Cuts at: 0 3 89 4386
Size: 3 86 4297
Fragments arranged by size:
4297 86 3
BanII G_rGCy'C
Cuts at: 0 63 770 3221 3914 4386
Size: 63 707 2451 693 472
Fragments arranged by size:
2451 707 693 472 63
BbeI G_GCGC'C
Cuts at: 0 93 4386
Size: 93 4293
BbsI GAAGACnn'nnnn_
Cuts at: 0 1639 4386
Size: 1639 2747
BbvCI CC'TCA_GC
Cuts at: 0 2435 4386
Size: 2435 1951
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3918 3952 4344 4378 4386
Size: 3918 34 392 34 8
Fragments arranged by size:
3918 392 34 34 8
BclI T'GATC_A
Cuts at: 0 927 3303 3989 4386
Size: 927 2376 686 397
Fragments arranged by size:
2376 927 686 397
BfrBI ATG'CAT
Cuts at: 0 2383 2955 4386
Size: 2383 572 1431
Fragments arranged by size:
2383 1431 572
Bme1580I G_kGCm'C
Cuts at: 0 14 590 4212 4386
Size: 14 576 3622 174
Fragments arranged by size:
3622 576 174 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 2455 3379 3618 3646 4109 4386
Size: 2455 924 239 28 463 277
Fragments arranged by size:
2455 924 463 277 239 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 814 2540 3533 4234 4386
Size: 618 196 1726 993 701 152
Fragments arranged by size:
1726 993 701 618 196 152
Bpu10I CC'TnA_GC
Cuts at: 0 2435 3584 4386
Size: 2435 1149 802
Fragments arranged by size:
2435 1149 802
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1379 3473 4282 4386
Size: 1379 2094 809 104
Fragments arranged by size:
2094 1379 809 104
BsaI GGTCTCn'nnnn_
Cuts at: 0 1105 4386
Size: 1105 3281
BsaHI Gr'CG_yC
Cuts at: 0 90 4386
Size: 90 4296
BsaWI w'CCGG_w
Cuts at: 0 2744 4184 4386
Size: 2744 1440 202
Fragments arranged by size:
2744 1440 202
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1200 1230 2513 2543 3350 3380 3506 3536
Size: 1200 30 1283 30 807 30 126 30
Cuts at: 3536 4386
Size: 850
Fragments arranged by size:
1283 1200 850 807 126 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1958 4386
Size: 1958 2428
BseYI C'CCAG_C
Cuts at: 0 2314 3638 3789 3913 4113 4169 4386
Size: 2314 1324 151 124 200 56 217
Fragments arranged by size:
2314 1324 217 200 151 124 56
BsiHKAI G_wGCw'C
Cuts at: 0 14 590 3221 4212 4386
Size: 14 576 2631 991 174
Fragments arranged by size:
2631 991 576 174 14
BsmI GAATG_Cn'
Cuts at: 0 1650 4386
Size: 1650 2736
Bsp1286I G_dGCh'C
Cuts at: 0 14 63 590 770 3221 3914 4212 4386
Size: 14 49 527 180 2451 693 298 174
Fragments arranged by size:
2451 693 527 298 180 174 49 14
BspEI T'CCGG_A
Cuts at: 0 2744 4386
Size: 2744 1642
BspHI T'CATG_A
Cuts at: 0 4233 4386
Size: 4233 153
BsrDI GCAATG_nn'
Cuts at: 0 413 743 1559 2293 3701 4386
Size: 413 330 816 734 1408 685
Fragments arranged by size:
1408 816 734 685 413 330
BsrFI r'CCGG_y
Cuts at: 0 4184 4386
Size: 4184 202
BsrGI T'GTAC_A
Cuts at: 0 696 2192 3095 3462 4163 4386
Size: 696 1496 903 367 701 223
Fragments arranged by size:
1496 903 701 696 367 223
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2515 4386
Size: 2515 1871
BstBI TT'CG_AA
Cuts at: 0 199 4386
Size: 199 4187
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1535 2913 4386
Size: 1535 1378 1473
Fragments arranged by size:
1535 1473 1378
BstYI r'GATC_y
Cuts at: 0 492 553 2690 2706 2741 3423 3644 3665
Size: 492 61 2137 16 35 682 221 21
Cuts at: 3665 4386
Size: 721
Fragments arranged by size:
2137 721 682 492 221 61 35 21
16
BtgI C'CryG_G
Cuts at: 0 596 770 4386
Size: 596 174 3616
Fragments arranged by size:
3616 596 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 704 2295 4386
Size: 704 1591 2091
Fragments arranged by size:
2091 1591 704
BtsI GCAGTG_nn'
Cuts at: 0 386 1849 3384 3557 4386
Size: 386 1463 1535 173 829
Fragments arranged by size:
1535 1463 829 386 173
ClaI AT'CG_AT
Cuts at: 0 3938 4386
Size: 3938 448
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1012 1047 1499 1534 4386
Size: 1012 35 452 35 2852
Fragments arranged by size:
2852 1012 452 35 35
EaeI y'GGCC_r
Cuts at: 0 270 3288 4386
Size: 270 3018 1098
Fragments arranged by size:
3018 1098 270
EarI CTCTTCn'nnn_
Cuts at: 0 713 776 3237 3334 3758 3803 4040 4366
Size: 713 63 2461 97 424 45 237 326
Cuts at: 4366 4386
Size: 20
Fragments arranged by size:
2461 713 424 326 237 97 63 45
20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 874 4386
Size: 874 3512
EcoICRI GAG'CTC
Cuts at: 0 3219 4386
Size: 3219 1167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 593 618 814 2014 2540 2633 2803
Size: 562 31 25 196 1200 526 93 170
Cuts at: 2803 3533 4234 4386
Size: 730 701 152
Fragments arranged by size:
1200 730 701 562 526 196 170 152
93 31 25
EcoO109I rG'GnC_Cy
Cuts at: 0 1483 3572 4386
Size: 1483 2089 814
Fragments arranged by size:
2089 1483 814
EcoRV GAT'ATC
Cuts at: 0 1896 4386
Size: 1896 2490
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1749 2663 4150 4386
Size: 1749 914 1487 236
Fragments arranged by size:
1749 1487 914 236
HaeII r_GCGC'y
Cuts at: 0 93 4386
Size: 93 4293
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 712 744 3172 3204 3408 3440
Size: 13 32 667 32 2428 32 204 32
Cuts at: 3440 3471 3503 3508 3539 3540 3571 4386
Size: 31 32 5 31 1 31 815
Fragments arranged by size:
2428 815 667 204 32 32 32 32
32 31 31 31 13 5 1
HincII GTy'rAC
Cuts at: 0 1990 4386
Size: 1990 2396
HindIII A'AGCT_T
Cuts at: 0 2014 2712 4386
Size: 2014 698 1674
Fragments arranged by size:
2014 1674 698
KasI G'GCGC_C
Cuts at: 0 89 4386
Size: 89 4297
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1016 2027 2204 3170 4386
Size: 1016 1011 177 966 1216
Fragments arranged by size:
1216 1016 1011 966 177
MscI TGG'CCA
Cuts at: 0 3290 4386
Size: 3290 1096
MslI CAynn'nnrTG
Cuts at: 0 1661 1748 3322 3464 3758 4386
Size: 1661 87 1574 142 294 628
Fragments arranged by size:
1661 1574 628 294 142 87
MspA1I CmG'CkG
Cuts at: 0 3591 4173 4386
Size: 3591 582 213
Fragments arranged by size:
3591 582 213
NarI GG'CG_CC
Cuts at: 0 90 4386
Size: 90 4296
NcoI C'CATG_G
Cuts at: 0 770 4386
Size: 770 3616
NdeI CA'TA_TG
Cuts at: 0 1785 3528 4386
Size: 1785 1743 858
Fragments arranged by size:
1785 1743 858
NsiI A_TGCA'T
Cuts at: 0 2385 2957 4386
Size: 2385 572 1429
Fragments arranged by size:
2385 1429 572
NspI r_CATG'y
Cuts at: 0 2010 3469 3857 4386
Size: 2010 1459 388 529
Fragments arranged by size:
2010 1459 529 388
PciI A'CATG_T
Cuts at: 0 3465 3853 4386
Size: 3465 388 533
Fragments arranged by size:
3465 533 388
PflMI CCAn_nnn'nTGG
Cuts at: 0 815 2017 4386
Size: 815 1202 2369
Fragments arranged by size:
2369 1202 815
PfoI T'CCnGG_A
Cuts at: 0 902 2329 4386
Size: 902 1427 2057
Fragments arranged by size:
2057 1427 902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4089 4121 4386
Size: 4089 32 265
Fragments arranged by size:
4089 265 32
PpuMI rG'GwC_Cy
Cuts at: 0 1483 4386
Size: 1483 2903
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1919 1951 4386
Size: 1919 32 2435
Fragments arranged by size:
2435 1919 32
PstI C_TGCA'G
Cuts at: 0 441 1946 3596 4283 4386
Size: 441 1505 1650 687 103
Fragments arranged by size:
1650 1505 687 441 103
PvuII CAG'CTG
Cuts at: 0 3591 4173 4386
Size: 3591 582 213
Fragments arranged by size:
3591 582 213
SacI G_AGCT'C
Cuts at: 0 3221 4386
Size: 3221 1165
SalI G'TCGA_C
Cuts at: 0 1988 4386
Size: 1988 2398
SapI GCTCTTCn'nnn_
Cuts at: 0 713 4040 4386
Size: 713 3327 346
Fragments arranged by size:
3327 713 346
ScaI AGT'ACT
Cuts at: 0 1354 1937 3628 4021 4386
Size: 1354 583 1691 393 365
Fragments arranged by size:
1691 1354 583 393 365
SexAI A'CCwGG_T
Cuts at: 0 1065 1571 4386
Size: 1065 506 2815
Fragments arranged by size:
2815 1065 506
SfcI C'TryA_G
Cuts at: 0 437 449 487 1942 2889 3320 3592 4279
Size: 437 12 38 1455 947 431 272 687
Cuts at: 4279 4287 4386
Size: 8 99
Fragments arranged by size:
1455 947 687 437 431 272 99 38
12 8
SfoI GGC'GCC
Cuts at: 0 91 4386
Size: 91 4295
SmlI C'TyrA_G
Cuts at: 0 56 1394 3488 4297 4386
Size: 56 1338 2094 809 89
Fragments arranged by size:
2094 1338 809 89 56
SspI AAT'ATT
Cuts at: 0 1914 3870 4386
Size: 1914 1956 516
Fragments arranged by size:
1956 1914 516
StyI C'CwwG_G
Cuts at: 0 461 770 809 2921 4386
Size: 461 309 39 2112 1465
Fragments arranged by size:
2112 1465 461 309 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2332 3637 3665 3745 4183 4386
Size: 2332 1305 28 80 438 203
Fragments arranged by size:
2332 1305 438 203 80 28
TatI w'GTAC_w
Cuts at: 0 696 709 1055 1352 1935 2192 2634 3095
Size: 696 13 346 297 583 257 442 461
Cuts at: 3095 3462 3626 3696 3969 4019 4163 4386
Size: 367 164 70 273 50 144 223
Fragments arranged by size:
696 583 461 442 367 346 297 273
257 223 164 144 70 50 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 588 620 3266 3298 3350 3382 3506 3538
Size: 588 32 2646 32 52 32 124 32
Cuts at: 3538 4386
Size: 848
Fragments arranged by size:
2646 848 588 124 52 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 879 4386
Size: 879 3507
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2337 4386
Size: 2337 2049
XhoI C'TCGA_G
Cuts at: 0 56 4386
Size: 56 4330
XmnI GAAnn'nnTTC
Cuts at: 0 577 2099 2132 3332 4386
Size: 577 1522 33 1200 1054
Fragments arranged by size:
1522 1200 1054 577 33
Enzymes that do cut:
AccI AcuI AflIII AgeI AhdI AleI AloI AlwNI
ApaLI ApoI AseI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BclI BfrBI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspEI BspHI BsrDI BsrFI BsrGI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV
FalI HaeII Hin4I HincII HindIII KasI MmeI MscI
MslI MspA1I NarI NcoI NdeI NsiI NspI PciI
PflMI PfoI PpiI PpuMI PsrI PstI PvuII SacI
SalI SapI ScaI SexAI SfcI SfoI SmlI SspI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AlfI ApaI
AscI AsiSI AvrII BaeI BciVI BglI BglII BlpI
BmgBI BmtI BplI BsaAI BsaBI BsgI BsiEI BsiWI
BsmBI BspMI BsrBI BssHII BssSI BstEII BstZ17I Bsu36I
DraI DraIII DrdI EagI EcoNI EcoRI FseI FspI
FspAI HpaI KpnI MfeI MluI NaeI NgoMIV NheI
NotI NruI PacI PasI PmeI PmlI PshAI PsiI
PspOMI PspXI PvuI RsrII SacII SanDI SbfI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI XbaI XmaI ZraI