(Linear) (Six-base) MAPSORT of: KIEE2669.seq  Check: 6035  from: 1  to: 1062



KIEE2669.orf

 With 182 enzymes: SgfI * 

                        January 26, 2017 16:50  ..

AccI GT'mk_AC

Cuts at:      0     867    1062 
   Size:        867     195
 
AleI CACnn'nnGTG

Cuts at:      0      73     256    1062 
   Size:         73     183     806

  Fragments arranged by size: 

                 806     183      73
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      20      52    1062 
   Size:         20      32    1010

  Fragments arranged by size: 

                1010      32      20
 
ApoI r'AATT_y

Cuts at:      0     967    1062 
   Size:        967      95
 
BamHI G'GATC_C

Cuts at:      0     936    1062 
   Size:        936     126
 
BanI G'GyrC_C

Cuts at:      0     104     318    1062 
   Size:        104     214     744

  Fragments arranged by size: 

                 744     214     104
 
BanII G_rGCy'C

Cuts at:      0     407    1062 
   Size:        407     655
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      24     780    1062 
   Size:         24     756     282

  Fragments arranged by size: 

                 756     282      24
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     545     579    1062 
   Size:        545      34     483

  Fragments arranged by size: 

                 545     483      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      46    1062 
   Size:         46    1016
 
BglI GCCn_nnn'nGGC

Cuts at:      0     603    1062 
   Size:        603     459
 
BglII A'GATC_T

Cuts at:      0      94    1062 
   Size:         94     968
 
Bme1580I G_kGCm'C

Cuts at:      0     109    1062 
   Size:        109     953
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     561    1062 
   Size:        412     149     501

  Fragments arranged by size: 

                 501     412     149
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     367     492    1062 
   Size:        367     125     570

  Fragments arranged by size: 

                 570     367     125
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     213    1062 
   Size:        213     849
 
BseYI C'CCAG_C

Cuts at:      0     141     217    1062 
   Size:        141      76     845

  Fragments arranged by size: 

                 845     141      76
 
Bsp1286I G_dGCh'C

Cuts at:      0     109     407    1062 
   Size:        109     298     655

  Fragments arranged by size: 

                 655     298     109
 
BsrBI CCG'CTC

Cuts at:      0     102    1062 
   Size:        102     960
 
BsrDI GCAATG_nn'

Cuts at:      0      68    1062 
   Size:         68     994
 
BsrFI r'CCGG_y

Cuts at:      0     169    1062 
   Size:        169     893
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     384    1062 
   Size:        384     678
 
BstYI r'GATC_y

Cuts at:      0      94     936    1062 
   Size:         94     842     126

  Fragments arranged by size: 

                 842     126      94
 
Bsu36I CC'TnA_GG

Cuts at:      0    1056    1062 
   Size:       1056       6
 
EaeI y'GGCC_r

Cuts at:      0     260    1062 
   Size:        260     802
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      43    1062 
   Size:         43    1019
 
EcoO109I rG'GnC_Cy

Cuts at:      0     595    1062 
   Size:        595     467
 
MslI CAynn'nnrTG

Cuts at:      0      73      76     256     382     931    1062 
   Size:         73       3     180     126     549     131

  Fragments arranged by size: 

                 549     180     131     126      73       3
 
MspA1I CmG'CkG

Cuts at:      0     132    1062 
   Size:        132     930
 
NdeI CA'TA_TG

Cuts at:      0     634    1062 
   Size:        634     428
 
PvuII CAG'CTG

Cuts at:      0     132    1062 
   Size:        132     930
 
SfcI C'TryA_G

Cuts at:      0     864    1062 
   Size:        864     198
 
SmlI C'TyrA_G

Cuts at:      0     427     540    1062 
   Size:        427     113     522

  Fragments arranged by size: 

                 522     427     113
 
SspI AAT'ATT

Cuts at:      0     453    1062 
   Size:        453     609
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     189    1062 
   Size:        189     873
 
TatI w'GTAC_w

Cuts at:      0     126    1062 
   Size:        126     936
 

 Enzymes that do cut:

     AccI     AleI     AloI     ApoI    BamHI     BanI    BanII     BbsI
     BcgI    BciVI     BglI    BglII Bme1580I    BpuEI     BsaI    BseRI
    BseYI Bsp1286I    BsrBI    BsrDI    BsrFI    BstXI    BstYI   Bsu36I
     EaeI    EcoNI EcoO109I     MslI   MspA1I     NdeI    PvuII     SfcI
     SmlI     SspI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BbeI    BbvCI     BclI    BfrBI
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI