Restriction map for
pFN21AE2650
(Linear) (Six-base) MAPSORT of: KIEE2650.seq Check: 8017 from: 1 to: 2595
KIEE2650.orf
With 182 enzymes: SgfI *
November 16, 2016 14:56 ..
AccI GT'mk_AC
Cuts at: 0 1512 2595
Size: 1512 1083
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1247 1301 2595
Size: 1247 54 1294
Fragments arranged by size:
1294 1247 54
AflII C'TTAA_G
Cuts at: 0 1756 2595
Size: 1756 839
AlwNI CAG_nnn'CTG
Cuts at: 0 97 2595
Size: 97 2498
ApaLI G'TGCA_C
Cuts at: 0 1585 2595
Size: 1585 1010
ApoI r'AATT_y
Cuts at: 0 31 179 273 393 577 616 646 823
Size: 31 148 94 120 184 39 30 177
Cuts at: 823 901 992 1523 1725 1899 1938 2014 2117
Size: 78 91 531 202 174 39 76 103
Cuts at: 2117 2287 2295 2533 2595
Size: 170 8 238 62
Fragments arranged by size:
531 238 202 184 177 174 170 148
120 103 94 91 78 76 62 39
39 31 30 8
AseI AT'TA_AT
Cuts at: 0 1865 2595
Size: 1865 730
BanII G_rGCy'C
Cuts at: 0 1229 1459 2595
Size: 1229 230 1136
Fragments arranged by size:
1229 1136 230
BbsI GAAGACnn'nnnn_
Cuts at: 0 780 2421 2595
Size: 780 1641 174
Fragments arranged by size:
1641 780 174
BciVI GTATCCnnnnn_n'
Cuts at: 0 2019 2595
Size: 2019 576
BglI GCCn_nnn'nGGC
Cuts at: 0 1233 2485 2595
Size: 1233 1252 110
Fragments arranged by size:
1252 1233 110
BglII A'GATC_T
Cuts at: 0 882 2086 2595
Size: 882 1204 509
Fragments arranged by size:
1204 882 509
BlpI GC'TnA_GC
Cuts at: 0 1097 2595
Size: 1097 1498
Bme1580I G_kGCm'C
Cuts at: 0 1589 1622 2595
Size: 1589 33 973
Fragments arranged by size:
1589 973 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 2587 2595
Size: 2587 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 230 2282 2595
Size: 230 2052 313
Fragments arranged by size:
2052 313 230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 672 1061 1228 1258 2595
Size: 270 402 389 167 30 1337
Fragments arranged by size:
1337 402 389 270 167 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1083 1112 1869 2233 2586 2595
Size: 1083 29 757 364 353 9
Fragments arranged by size:
1083 757 364 353 29 9
BsaAI yAC'GTr
Cuts at: 0 1510 2595
Size: 1510 1085
BsaBI GATnn'nnATC
Cuts at: 0 557 2595
Size: 557 2038
BsaWI w'CCGG_w
Cuts at: 0 114 2595
Size: 114 2481
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 685 1120 1168 1225 1228 1231 1255
Size: 214 471 435 48 57 3 3 24
Cuts at: 1255 1288 1312 1450 1903 2095 2595
Size: 33 24 138 453 192 500
Fragments arranged by size:
500 471 453 435 214 192 138 57
48 33 24 24 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1774 2595
Size: 1774 821
BsiHKAI G_wGCw'C
Cuts at: 0 1459 1589 2595
Size: 1459 130 1006
Fragments arranged by size:
1459 1006 130
BsmI GAATG_Cn'
Cuts at: 0 1713 2595
Size: 1713 882
Bsp1286I G_dGCh'C
Cuts at: 0 1229 1459 1589 1622 2595
Size: 1229 230 130 33 973
Fragments arranged by size:
1229 973 230 130 33
BspHI T'CATG_A
Cuts at: 0 1695 2595
Size: 1695 900
BsrDI GCAATG_nn'
Cuts at: 0 570 781 2430 2595
Size: 570 211 1649 165
Fragments arranged by size:
1649 570 211 165
BsrGI T'GTAC_A
Cuts at: 0 21 2595
Size: 21 2574
BstYI r'GATC_y
Cuts at: 0 882 2086 2595
Size: 882 1204 509
Fragments arranged by size:
1204 882 509
Bsu36I CC'TnA_GG
Cuts at: 0 263 1173 1200 1230 1287 2595
Size: 263 910 27 30 57 1308
Fragments arranged by size:
1308 910 263 57 30 27
DraI TTT'AAA
Cuts at: 0 128 405 680 852 2595
Size: 128 277 275 172 1743
Fragments arranged by size:
1743 277 275 172 128
DraIII CAC_nnn'GTG
Cuts at: 0 1581 2595
Size: 1581 1014
EarI CTCTTCn'nnn_
Cuts at: 0 1130 1160 1217 1250 1298 1598 2334 2595
Size: 1130 30 57 33 48 300 736 261
Fragments arranged by size:
1130 736 300 261 57 48 33 30
EcoICRI GAG'CTC
Cuts at: 0 1457 2595
Size: 1457 1138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 672 1061 1228 1247 1258 1301 2595
Size: 270 402 389 167 19 11 43 1294
Fragments arranged by size:
1294 402 389 270 167 43 19 11
EcoRI G'AATT_C
Cuts at: 0 273 992 2014 2117 2295 2595
Size: 273 719 1022 103 178 300
Fragments arranged by size:
1022 719 300 273 178 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 733 765 1270 1302 1414 1446 2071 2103
Size: 733 32 505 32 112 32 625 32
Cuts at: 2103 2595
Size: 492
Fragments arranged by size:
733 625 505 492 112 32 32 32
32
HincII GTy'rAC
Cuts at: 0 89 2375 2595
Size: 89 2286 220
Fragments arranged by size:
2286 220 89
HpaI GTT'AAC
Cuts at: 0 89 2595
Size: 89 2506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1250 2595
Size: 1250 1345
MslI CAynn'nnrTG
Cuts at: 0 70 852 1919 2281 2595
Size: 70 782 1067 362 314
Fragments arranged by size:
1067 782 362 314 70
MspA1I CmG'CkG
Cuts at: 0 1615 2595
Size: 1615 980
NdeI CA'TA_TG
Cuts at: 0 2555 2595
Size: 2555 40
PasI CC'CwG_GG
Cuts at: 0 483 2595
Size: 483 2112
PvuII CAG'CTG
Cuts at: 0 1615 2595
Size: 1615 980
SacI G_AGCT'C
Cuts at: 0 1459 2595
Size: 1459 1136
SapI GCTCTTCn'nnn_
Cuts at: 0 1217 2595
Size: 1217 1378
ScaI AGT'ACT
Cuts at: 0 725 2595
Size: 725 1870
SexAI A'CCwGG_T
Cuts at: 0 1577 2595
Size: 1577 1018
SfcI C'TryA_G
Cuts at: 0 1415 2595
Size: 1415 1180
SmlI C'TyrA_G
Cuts at: 0 1062 1127 1756 1848 2212 2565 2595
Size: 1062 65 629 92 364 353 30
Fragments arranged by size:
1062 629 364 353 92 65 30
SpeI A'CTAG_T
Cuts at: 0 16 2595
Size: 16 2579
StuI AGG'CCT
Cuts at: 0 1171 1285 2595
Size: 1171 114 1310
Fragments arranged by size:
1310 1171 114
StyI C'CwwG_G
Cuts at: 0 2355 2595
Size: 2355 240
SwaI ATTT'AAAT
Cuts at: 0 852 2595
Size: 852 1743
TatI w'GTAC_w
Cuts at: 0 21 723 945 2595
Size: 21 702 222 1650
Fragments arranged by size:
1650 702 222 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2286 2318 2595
Size: 2286 32 277
Fragments arranged by size:
2286 277 32
XbaI T'CTAG_A
Cuts at: 0 1995 2089 2329 2595
Size: 1995 94 240 266
Fragments arranged by size:
1995 266 240 94
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1389 2595
Size: 1389 1206
Enzymes that do cut:
AccI AcuI AflII AlwNI ApaLI ApoI AseI BanII
BbsI BciVI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaAI BsaBI BsaWI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspHI BsrDI BsrGI BstYI Bsu36I DraI
DraIII EarI EcoICRI Eco57MI EcoRI Hin4I HincII HpaI
MmeI MslI MspA1I NdeI PasI PvuII SacI SapI
ScaI SexAI SfcI SmlI SpeI StuI StyI SwaI
TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BclI BfrBI
BmgBI BmtI Bpu10I BsaI BsaHI BsaXI BseYI BsiEI
BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DrdI EaeI EagI EciI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HaeII HindIII KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI
SrfI SspI TaqII Tth111I XhoI XmaI XmnI ZraI