Restriction map for
pFN21AE2647
(Linear) (Six-base) MAPSORT of: KIEE2647.seq Check: 3636 from: 1 to: 1476
KIEE2647.orf
With 182 enzymes: SgfI *
November 11, 2016 16:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 552 1476
Size: 552 924
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 1476
Size: 124 1352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 538 841 1476
Size: 538 303 635
Fragments arranged by size:
635 538 303
AlwNI CAG_nnn'CTG
Cuts at: 0 623 1302 1476
Size: 623 679 174
Fragments arranged by size:
679 623 174
ApaI G_GGCC'C
Cuts at: 0 763 1476
Size: 763 713
ApaLI G'TGCA_C
Cuts at: 0 283 565 1476
Size: 283 282 911
Fragments arranged by size:
911 283 282
AvaI C'yCGr_G
Cuts at: 0 7 49 556 1096 1476
Size: 7 42 507 540 380
Fragments arranged by size:
540 507 380 42 7
BanI G'GyrC_C
Cuts at: 0 448 817 1236 1476
Size: 448 369 419 240
Fragments arranged by size:
448 419 369 240
BanII G_rGCy'C
Cuts at: 0 8 136 557 763 1056 1476
Size: 8 128 421 206 293 420
Fragments arranged by size:
421 420 293 206 128 8
BbeI G_GCGC'C
Cuts at: 0 452 1476
Size: 452 1024
BbsI GAAGACnn'nnnn_
Cuts at: 0 611 1476
Size: 611 865
BclI T'GATC_A
Cuts at: 0 431 1476
Size: 431 1045
BglI GCCn_nnn'nGGC
Cuts at: 0 394 649 758 772 934 943 1021 1168
Size: 394 255 109 14 162 9 78 147
Cuts at: 1168 1476
Size: 308
Fragments arranged by size:
394 308 255 162 147 109 78 14
9
BglII A'GATC_T
Cuts at: 0 1322 1476
Size: 1322 154
Bme1580I G_kGCm'C
Cuts at: 0 262 287 569 763 1476
Size: 262 25 282 194 713
Fragments arranged by size:
713 282 262 194 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 175 1476
Size: 175 1301
BsaHI Gr'CG_yC
Cuts at: 0 449 920 1476
Size: 449 471 556
Fragments arranged by size:
556 471 449
BsaWI w'CCGG_w
Cuts at: 0 184 1476
Size: 184 1292
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 473 503 669 699 865 895 1476
Size: 473 30 166 30 166 30 581
Fragments arranged by size:
581 473 166 166 30 30 30
BseYI C'CCAG_C
Cuts at: 0 118 756 951 1476
Size: 118 638 195 525
Fragments arranged by size:
638 525 195 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 187 511 610 1476
Size: 45 142 324 99 866
Fragments arranged by size:
866 324 142 99 45
BsiEI CG_ry'CG
Cuts at: 0 725 737 908 1163 1172 1476
Size: 725 12 171 255 9 304
Fragments arranged by size:
725 304 255 171 12 9
BsiHKAI G_wGCw'C
Cuts at: 0 287 569 1476
Size: 287 282 907
Fragments arranged by size:
907 287 282
BsiWI C'GTAC_G
Cuts at: 0 689 1476
Size: 689 787
BsmI GAATG_Cn'
Cuts at: 0 282 1476
Size: 282 1194
Bsp1286I G_dGCh'C
Cuts at: 0 8 136 262 287 557 569 763 1056
Size: 8 128 126 25 270 12 194 293
Cuts at: 1056 1476
Size: 420
Fragments arranged by size:
420 293 270 194 128 126 25 12
8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 530 552 833 1476
Size: 530 22 281 643
Fragments arranged by size:
643 530 281 22
BsrBI CCG'CTC
Cuts at: 0 240 1405 1476
Size: 240 1165 71
Fragments arranged by size:
1165 240 71
BsrFI r'CCGG_y
Cuts at: 0 679 1361 1476
Size: 679 682 115
Fragments arranged by size:
682 679 115
BstAPI GCAn_nnn'nTGC
Cuts at: 0 623 1476
Size: 623 853
BstEII G'GTnAC_C
Cuts at: 0 1368 1428 1476
Size: 1368 60 48
Fragments arranged by size:
1368 60 48
BstXI CCAn_nnnn'nTGG
Cuts at: 0 885 978 1221 1476
Size: 885 93 243 255
Fragments arranged by size:
885 255 243 93
BstYI r'GATC_y
Cuts at: 0 1322 1476
Size: 1322 154
Bsu36I CC'TnA_GG
Cuts at: 0 338 1476
Size: 338 1138
BtgI C'CryG_G
Cuts at: 0 452 659 701 719 932 1157 1388 1476
Size: 452 207 42 18 213 225 231 88
Fragments arranged by size:
452 231 225 213 207 88 42 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 340 1476
Size: 340 1136
EaeI y'GGCC_r
Cuts at: 0 662 722 734 830 905 926 935 1073
Size: 662 60 12 96 75 21 9 138
Cuts at: 1073 1145 1160 1169 1391 1476
Size: 72 15 9 222 85
Fragments arranged by size:
662 222 138 96 85 75 72 60
21 15 12 9 9
EagI C'GGCC_G
Cuts at: 0 722 734 905 1160 1169 1476
Size: 722 12 171 255 9 307
Fragments arranged by size:
722 307 255 171 12 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1139 1204 1476
Size: 1139 65 272
Fragments arranged by size:
1139 272 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 1476
Size: 124 1352
EcoNI CCTnn'n_nnAGG
Cuts at: 0 253 1267 1476
Size: 253 1014 209
Fragments arranged by size:
1014 253 209
EcoO109I rG'GnC_Cy
Cuts at: 0 12 32 655 760 785 1061 1451 1476
Size: 12 20 623 105 25 276 390 25
Fragments arranged by size:
623 390 276 105 25 25 20 12
HaeII r_GCGC'y
Cuts at: 0 452 584 847 1313 1476
Size: 452 132 263 466 163
Fragments arranged by size:
466 452 263 163 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 179 211 1296 1328 1476
Size: 179 32 1085 32 148
Fragments arranged by size:
1085 179 148 32 32
HincII GTy'rAC
Cuts at: 0 348 1476
Size: 348 1128
KasI G'GCGC_C
Cuts at: 0 448 1476
Size: 448 1028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 1476
Size: 695 781
MscI TGG'CCA
Cuts at: 0 664 832 1476
Size: 664 168 644
Fragments arranged by size:
664 644 168
MspA1I CmG'CkG
Cuts at: 0 171 495 548 623 703 721 1140 1150
Size: 171 324 53 75 80 18 419 10
Cuts at: 1150 1159 1390 1425 1476
Size: 9 231 35 51
Fragments arranged by size:
419 324 231 171 80 75 53 51
35 18 10 9
NarI GG'CG_CC
Cuts at: 0 449 1476
Size: 449 1027
NotI GC'GGCC_GC
Cuts at: 0 722 1476
Size: 722 754
PasI CC'CwG_GG
Cuts at: 0 885 1056 1476
Size: 885 171 420
Fragments arranged by size:
885 420 171
PflMI CCAn_nnn'nTGG
Cuts at: 0 1470 1476
Size: 1470 6
PfoI T'CCnGG_A
Cuts at: 0 1047 1476
Size: 1047 429
PpuMI rG'GwC_Cy
Cuts at: 0 1061 1476
Size: 1061 415
PspOMI G'GGCC_C
Cuts at: 0 759 1476
Size: 759 717
PstI C_TGCA'G
Cuts at: 0 176 527 1155 1476
Size: 176 351 628 321
Fragments arranged by size:
628 351 321 176
PvuII CAG'CTG
Cuts at: 0 171 495 623 1476
Size: 171 324 128 853
Fragments arranged by size:
853 324 171 128
SacII CC_GC'GG
Cuts at: 0 704 722 1160 1391 1476
Size: 704 18 438 231 85
Fragments arranged by size:
704 438 231 85 18
SbfI CC_TGCA'GG
Cuts at: 0 527 1476
Size: 527 949
SexAI A'CCwGG_T
Cuts at: 0 1247 1476
Size: 1247 229
SfcI C'TryA_G
Cuts at: 0 172 523 1151 1476
Size: 172 351 628 325
Fragments arranged by size:
628 351 325 172
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 758 934 943 1168 1476
Size: 758 176 9 225 308
Fragments arranged by size:
758 308 225 176 9
SfoI GGC'GCC
Cuts at: 0 450 1476
Size: 450 1026
StuI AGG'CCT
Cuts at: 0 271 1476
Size: 271 1205
StyI C'CwwG_G
Cuts at: 0 1214 1476
Size: 1214 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 477 1476
Size: 477 999
TatI w'GTAC_w
Cuts at: 0 252 1476
Size: 252 1224
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 473 505 669 701 1476
Size: 473 32 164 32 775
Fragments arranged by size:
775 473 164 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 339 1221 1476
Size: 339 882 255
Fragments arranged by size:
882 339 255
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI ApaLI AvaI BanI
BanII BbeI BbsI BclI BglI BglII Bme1580I BsaI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI EaeI EagI EciI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MscI
MspA1I NarI NotI PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SacII SbfI SexAI SfcI SfiI SfoI
StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BfrBI BlpI BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI
BseRI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstBI BstZ17I BtsI ClaI CspCI DraI DraIII DrdI
EarI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MslI NaeI NcoI
NdeI NgoMIV NheI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SanDI SapI ScaI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI