Restriction map for
pFN21AE2642
(Linear) (Six-base) MAPSORT of: KIEE2642.seq Check: 1462 from: 1 to: 1191
KIEE2642.orf
With 182 enzymes: SgfI *
November 2, 2016 14:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 659 705 1191
Size: 659 46 486
Fragments arranged by size:
659 486 46
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 660 1133 1191
Size: 305 355 473 58
Fragments arranged by size:
473 355 305 58
AleI CACnn'nnGTG
Cuts at: 0 886 1191
Size: 886 305
AlwNI CAG_nnn'CTG
Cuts at: 0 459 543 1191
Size: 459 84 648
Fragments arranged by size:
648 459 84
ApaI G_GGCC'C
Cuts at: 0 684 1191
Size: 684 507
ApoI r'AATT_y
Cuts at: 0 912 1191
Size: 912 279
AseI AT'TA_AT
Cuts at: 0 389 1191
Size: 389 802
AvaI C'yCGr_G
Cuts at: 0 298 584 1191
Size: 298 286 607
Fragments arranged by size:
607 298 286
BanII G_rGCy'C
Cuts at: 0 684 1191
Size: 684 507
BbsI GAAGACnn'nnnn_
Cuts at: 0 293 350 443 992 1121 1191
Size: 293 57 93 549 129 70
Fragments arranged by size:
549 293 129 93 70 57
BbvCI CC'TCA_GC
Cuts at: 0 506 1191
Size: 506 685
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 799 833 1191
Size: 799 34 358
Fragments arranged by size:
799 358 34
BlpI GC'TnA_GC
Cuts at: 0 856 1191
Size: 856 335
Bme1580I G_kGCm'C
Cuts at: 0 684 1191
Size: 684 507
BmgBI CAC'GTC
Cuts at: 0 91 396 1119 1191
Size: 91 305 723 72
Fragments arranged by size:
723 305 91 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 933 1191
Size: 933 258
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 451 952 1191
Size: 100 351 501 239
Fragments arranged by size:
501 351 239 100
Bpu10I CC'TnA_GC
Cuts at: 0 506 698 1191
Size: 506 192 493
Fragments arranged by size:
506 493 192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 1075 1191
Size: 185 890 116
Fragments arranged by size:
890 185 116
BsaI GGTCTCn'nnnn_
Cuts at: 0 663 928 1191
Size: 663 265 263
Fragments arranged by size:
663 265 263
BsaWI w'CCGG_w
Cuts at: 0 599 1023 1191
Size: 599 424 168
Fragments arranged by size:
599 424 168
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 925 955 1191
Size: 925 30 236
Fragments arranged by size:
925 236 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 44 221 316 325 367 421 784 1191
Size: 44 177 95 9 42 54 363 407
Fragments arranged by size:
407 363 177 95 54 44 42 9
BseYI C'CCAG_C
Cuts at: 0 107 293 590 1191
Size: 107 186 297 601
Fragments arranged by size:
601 297 186 107
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 733 1191
Size: 733 458
BsiHKAI G_wGCw'C
Cuts at: 0 121 1191
Size: 121 1070
BsmI GAATG_Cn'
Cuts at: 0 243 750 1191
Size: 243 507 441
Fragments arranged by size:
507 441 243
BsmBI CGTCTCn'nnnn_
Cuts at: 0 81 1191
Size: 81 1110
Bsp1286I G_dGCh'C
Cuts at: 0 121 684 1191
Size: 121 563 507
Fragments arranged by size:
563 507 121
BspEI T'CCGG_A
Cuts at: 0 1023 1191
Size: 1023 168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 227 581 659 705 1191
Size: 227 354 78 46 486
Fragments arranged by size:
486 354 227 78 46
BsrBI CCG'CTC
Cuts at: 0 813 1191
Size: 813 378
BsrFI r'CCGG_y
Cuts at: 0 803 1191
Size: 803 388
BstAPI GCAn_nnn'nTGC
Cuts at: 0 552 1191
Size: 552 639
Bsu36I CC'TnA_GG
Cuts at: 0 1147 1191
Size: 1147 44
DraI TTT'AAA
Cuts at: 0 15 1191
Size: 15 1176
DraIII CAC_nnn'GTG
Cuts at: 0 9 1053 1191
Size: 9 1044 138
Fragments arranged by size:
1044 138 9
EaeI y'GGCC_r
Cuts at: 0 450 638 665 1191
Size: 450 188 27 526
Fragments arranged by size:
526 450 188 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 286 679 1191
Size: 286 393 512
Fragments arranged by size:
512 393 286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 305 451 660 952 1133 1191
Size: 100 205 146 209 292 181 58
Fragments arranged by size:
292 209 205 181 146 100 58
EcoO109I rG'GnC_Cy
Cuts at: 0 111 372 680 681 1191
Size: 111 261 308 1 510
Fragments arranged by size:
510 308 261 111 1
HaeII r_GCGC'y
Cuts at: 0 598 1191
Size: 598 593
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1123 1155 1191
Size: 1123 32 36
Fragments arranged by size:
1123 36 32
HincII GTy'rAC
Cuts at: 0 1125 1191
Size: 1125 66
HindIII A'AGCT_T
Cuts at: 0 1029 1191
Size: 1029 162
HpaI GTT'AAC
Cuts at: 0 1125 1191
Size: 1125 66
MscI TGG'CCA
Cuts at: 0 640 667 1191
Size: 640 27 524
Fragments arranged by size:
640 524 27
MslI CAynn'nnrTG
Cuts at: 0 886 1191
Size: 886 305
MspA1I CmG'CkG
Cuts at: 0 107 429 1191
Size: 107 322 762
Fragments arranged by size:
762 322 107
NspI r_CATG'y
Cuts at: 0 706 1191
Size: 706 485
PflMI CCAn_nnn'nTGG
Cuts at: 0 654 1191
Size: 654 537
PpuMI rG'GwC_Cy
Cuts at: 0 372 1191
Size: 372 819
PspOMI G'GGCC_C
Cuts at: 0 680 1191
Size: 680 511
PspXI vC'TCGA_Gb
Cuts at: 0 298 1191
Size: 298 893
PstI C_TGCA'G
Cuts at: 0 73 106 224 464 548 1191
Size: 73 33 118 240 84 643
Fragments arranged by size:
643 240 118 84 73 33
PvuII CAG'CTG
Cuts at: 0 107 429 1191
Size: 107 322 762
Fragments arranged by size:
762 322 107
SbfI CC_TGCA'GG
Cuts at: 0 224 1191
Size: 224 967
SexAI A'CCwGG_T
Cuts at: 0 632 959 1191
Size: 632 327 232
Fragments arranged by size:
632 327 232
SfcI C'TryA_G
Cuts at: 0 69 102 220 460 544 785 1191
Size: 69 33 118 240 84 241 406
Fragments arranged by size:
406 241 240 118 84 69 33
SmlI C'TyrA_G
Cuts at: 0 200 298 1090 1191
Size: 200 98 792 101
Fragments arranged by size:
792 200 101 98
StuI AGG'CCT
Cuts at: 0 743 1088 1191
Size: 743 345 103
Fragments arranged by size:
743 345 103
StyI C'CwwG_G
Cuts at: 0 338 1100 1191
Size: 338 762 91
Fragments arranged by size:
762 338 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 83 307 1191
Size: 83 224 884
Fragments arranged by size:
884 224 83
TatI w'GTAC_w
Cuts at: 0 564 1175 1191
Size: 564 611 16
Fragments arranged by size:
611 564 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1039 1071 1191
Size: 1039 32 120
Fragments arranged by size:
1039 120 32
Tth111I GACn'n_nGTC
Cuts at: 0 516 1191
Size: 516 675
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 946 1191
Size: 946 245
XhoI C'TCGA_G
Cuts at: 0 298 1191
Size: 298 893
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI ApoI AseI AvaI
BanII BbsI BbvCI BcgI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrFI BstAPI Bsu36I DraI DraIII EaeI EciI Eco57MI
EcoO109I HaeII Hin4I HincII HindIII HpaI MscI MslI
MspA1I NspI PflMI PpuMI PspOMI PspXI PstI PvuII
SbfI SexAI SfcI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI AscI AsiSI AvrII BaeI
BamHI BanI BbeI BciVI BclI BfrBI BglI BglII
BmtI BplI BsaAI BsaBI BsaHI BsiEI BsiWI BspHI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DrdI EagI
EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XmaI XmnI ZraI