Restriction map for
pFN21AE2628
(Linear) (Six-base) MAPSORT of: KIEE2628.seq Check: 6948 from: 1 to: 1851
KIEE2628.orf
With 182 enzymes: SgfI *
June 10, 2016 16:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 15 1851
Size: 15 1836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1333 1335 1851
Size: 1333 2 516
Fragments arranged by size:
1333 516 2
AflIII A'CryG_T
Cuts at: 0 233 1851
Size: 233 1618
AlwNI CAG_nnn'CTG
Cuts at: 0 132 1311 1851
Size: 132 1179 540
Fragments arranged by size:
1179 540 132
ApoI r'AATT_y
Cuts at: 0 1000 1394 1851
Size: 1000 394 457
Fragments arranged by size:
1000 457 394
AvaI C'yCGr_G
Cuts at: 0 34 164 1851
Size: 34 130 1687
Fragments arranged by size:
1687 130 34
BanI G'GyrC_C
Cuts at: 0 138 274 1432 1704 1851
Size: 138 136 1158 272 147
Fragments arranged by size:
1158 272 147 138 136
BanII G_rGCy'C
Cuts at: 0 1747 1851
Size: 1747 104
BbeI G_GCGC'C
Cuts at: 0 142 1851
Size: 142 1709
BbsI GAAGACnn'nnnn_
Cuts at: 0 775 1851
Size: 775 1076
BbvCI CC'TCA_GC
Cuts at: 0 1732 1851
Size: 1732 119
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 55 89 168 202 1851
Size: 55 34 79 34 1649
Fragments arranged by size:
1649 79 55 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1181 1851
Size: 1181 670
BglI GCCn_nnn'nGGC
Cuts at: 0 702 1851
Size: 702 1149
Bme1580I G_kGCm'C
Cuts at: 0 1435 1709 1851
Size: 1435 274 142
Fragments arranged by size:
1435 274 142
BmrI ACTGGGnnnn_n'
Cuts at: 0 693 1674 1851
Size: 693 981 177
Fragments arranged by size:
981 693 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1569 1851
Size: 1569 282
Bpu10I CC'TnA_GC
Cuts at: 0 1732 1851
Size: 1732 119
BsaI GGTCTCn'nnnn_
Cuts at: 0 163 1851
Size: 163 1688
BsaHI Gr'CG_yC
Cuts at: 0 122 139 842 1343 1851
Size: 122 17 703 501 508
Fragments arranged by size:
703 508 501 122 17
BsaWI w'CCGG_w
Cuts at: 0 1322 1851
Size: 1322 529
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 475 505 1851
Size: 475 30 1346
Fragments arranged by size:
1346 475 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 483 559 1851
Size: 483 76 1292
Fragments arranged by size:
1292 483 76
BseYI C'CCAG_C
Cuts at: 0 1242 1851
Size: 1242 609
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 640 868 1851
Size: 49 591 228 983
Fragments arranged by size:
983 591 228 49
BsiHKAI G_wGCw'C
Cuts at: 0 830 1793 1851
Size: 830 963 58
Fragments arranged by size:
963 830 58
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1071 1851
Size: 1071 780
Bsp1286I G_dGCh'C
Cuts at: 0 830 1435 1709 1747 1793 1851
Size: 830 605 274 38 46 58
Fragments arranged by size:
830 605 274 58 46 38
BspHI T'CATG_A
Cuts at: 0 681 1851
Size: 681 1170
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 15 518 890 1851
Size: 15 503 372 961
Fragments arranged by size:
961 503 372 15
BsrFI r'CCGG_y
Cuts at: 0 624 1851
Size: 624 1227
BssHII G'CGCG_C
Cuts at: 0 79 1851
Size: 79 1772
BssSI C'ACGA_G
Cuts at: 0 664 1851
Size: 664 1187
BstAPI GCAn_nnn'nTGC
Cuts at: 0 711 1851
Size: 711 1140
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1668 1701 1851
Size: 1668 33 150
Fragments arranged by size:
1668 150 33
BstYI r'GATC_y
Cuts at: 0 1198 1851
Size: 1198 653
BtgI C'CryG_G
Cuts at: 0 837 914 1694 1851
Size: 837 77 780 157
Fragments arranged by size:
837 780 157 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 294 450 1665 1851
Size: 294 156 1215 186
Fragments arranged by size:
1215 294 186 156
BtsI GCAGTG_nn'
Cuts at: 0 224 1851
Size: 224 1627
DraIII CAC_nnn'GTG
Cuts at: 0 1800 1851
Size: 1800 51
DrdI GACnn_nn'nnGTC
Cuts at: 0 1306 1851
Size: 1306 545
EaeI y'GGCC_r
Cuts at: 0 227 528 1136 1844 1851
Size: 227 301 608 708 7
Fragments arranged by size:
708 608 301 227 7
EarI CTCTTCn'nnn_
Cuts at: 0 493 1336 1851
Size: 493 843 515
Fragments arranged by size:
843 515 493
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 64 1851
Size: 64 1787
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1333 1335 1569 1851
Size: 1333 2 234 282
Fragments arranged by size:
1333 282 234 2
EcoO109I rG'GnC_Cy
Cuts at: 0 1308 1851
Size: 1308 543
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 207 1851
Size: 207 1644
HaeII r_GCGC'y
Cuts at: 0 142 243 1243 1851
Size: 142 101 1000 608
Fragments arranged by size:
1000 608 142 101
HincII GTy'rAC
Cuts at: 0 1122 1851
Size: 1122 729
KasI G'GCGC_C
Cuts at: 0 138 1851
Size: 138 1713
MscI TGG'CCA
Cuts at: 0 229 530 1138 1846 1851
Size: 229 301 608 708 5
Fragments arranged by size:
708 608 301 229 5
MspA1I CmG'CkG
Cuts at: 0 674 1851
Size: 674 1177
NaeI GCC'GGC
Cuts at: 0 626 1851
Size: 626 1225
NarI GG'CG_CC
Cuts at: 0 139 1851
Size: 139 1712
NcoI C'CATG_G
Cuts at: 0 837 1694 1851
Size: 837 857 157
Fragments arranged by size:
857 837 157
NgoMIV G'CCGG_C
Cuts at: 0 624 1851
Size: 624 1227
PasI CC'CwG_GG
Cuts at: 0 1655 1851
Size: 1655 196
PflMI CCAn_nnn'nTGG
Cuts at: 0 236 380 920 1800 1851
Size: 236 144 540 880 51
Fragments arranged by size:
880 540 236 144 51
PfoI T'CCnGG_A
Cuts at: 0 1412 1851
Size: 1412 439
PpuMI rG'GwC_Cy
Cuts at: 0 1308 1851
Size: 1308 543
PshAI GACnn'nnGTC
Cuts at: 0 56 1851
Size: 56 1795
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 283 315 1851
Size: 283 32 1536
Fragments arranged by size:
1536 283 32
ScaI AGT'ACT
Cuts at: 0 604 1851
Size: 604 1247
SexAI A'CCwGG_T
Cuts at: 0 1796 1851
Size: 1796 55
SfoI GGC'GCC
Cuts at: 0 140 1851
Size: 140 1711
SmaI CCC'GGG
Cuts at: 0 166 1851
Size: 166 1685
StyI C'CwwG_G
Cuts at: 0 837 953 1092 1385 1694 1851
Size: 837 116 139 293 309 157
Fragments arranged by size:
837 309 293 157 139 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 535 1656 1851
Size: 535 1121 195
Fragments arranged by size:
1121 535 195
TatI w'GTAC_w
Cuts at: 0 353 602 611 1851
Size: 353 249 9 1240
Fragments arranged by size:
1240 353 249 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 510 542 770 802 1851
Size: 510 32 228 32 1049
Fragments arranged by size:
1049 510 228 32 32
XmaI C'CCGG_G
Cuts at: 0 164 1851
Size: 164 1687
XmnI GAAnn'nnTTC
Cuts at: 0 1330 1851
Size: 1330 521
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglI Bme1580I BmrI
BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrFI BssHII
BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI DraIII
DrdI EaeI EarI EciI Eco57MI EcoO109I FalI HaeII
HincII KasI MscI MspA1I NaeI NarI NcoI NgoMIV
PasI PflMI PfoI PpuMI PshAI PsrI ScaI SexAI
SfoI SmaI StyI TaqII TatI TstI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BclI BfrBI BglII BlpI BmgBI
BmtI BplI BpuEI BsaAI BsaBI BsiEI BsiWI BsmI
BspEI BsrBI BsrDI BsrGI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI EagI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI
MluI MmeI MslI NdeI NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI SfcI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XhoI ZraI