Restriction map for
pFN21AE2623
(Linear) (Six-base) MAPSORT of: KIEE2623.seq Check: 7503 from: 1 to: 681
KIEE2623.orf
With 182 enzymes: SgfI *
May 10, 2016 15:41 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 556 681
Size: 521 35 125
Fragments arranged by size:
521 125 35
AleI CACnn'nnGTG
Cuts at: 0 64 681
Size: 64 617
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 502 534 681
Size: 502 32 147
Fragments arranged by size:
502 147 32
AlwNI CAG_nnn'CTG
Cuts at: 0 172 681
Size: 172 509
ApoI r'AATT_y
Cuts at: 0 566 681
Size: 566 115
BanI G'GyrC_C
Cuts at: 0 160 681
Size: 160 521
BanII G_rGCy'C
Cuts at: 0 457 681
Size: 457 224
BbvCI CC'TCA_GC
Cuts at: 0 200 681
Size: 200 481
BfrBI ATG'CAT
Cuts at: 0 415 681
Size: 415 266
BmrI ACTGGGnnnn_n'
Cuts at: 0 621 681
Size: 621 60
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 471 681
Size: 471 210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 681
Size: 463 218
Bpu10I CC'TnA_GC
Cuts at: 0 200 353 681
Size: 200 153 328
Fragments arranged by size:
328 200 153
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 217 355 460 465 681
Size: 217 138 105 5 216
Fragments arranged by size:
217 216 138 105 5
BsiHKAI G_wGCw'C
Cuts at: 0 457 681
Size: 457 224
BsmI GAATG_Cn'
Cuts at: 0 415 681
Size: 415 266
Bsp1286I G_dGCh'C
Cuts at: 0 457 681
Size: 457 224
BsrBI CCG'CTC
Cuts at: 0 616 681
Size: 616 65
BstBI TT'CG_AA
Cuts at: 0 130 681
Size: 130 551
BstEII G'GTnAC_C
Cuts at: 0 27 681
Size: 27 654
BstXI CCAn_nnnn'nTGG
Cuts at: 0 270 681
Size: 270 411
BtgI C'CryG_G
Cuts at: 0 62 681
Size: 62 619
EaeI y'GGCC_r
Cuts at: 0 620 681
Size: 620 61
EarI CTCTTCn'nnn_
Cuts at: 0 186 377 497 681
Size: 186 191 120 184
Fragments arranged by size:
191 186 184 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 55 681
Size: 55 626
EcoICRI GAG'CTC
Cuts at: 0 455 681
Size: 455 226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 521 556 681
Size: 463 58 35 125
Fragments arranged by size:
463 125 58 35
HincII GTy'rAC
Cuts at: 0 305 681
Size: 305 376
HpaI GTT'AAC
Cuts at: 0 305 681
Size: 305 376
MscI TGG'CCA
Cuts at: 0 622 681
Size: 622 59
MslI CAynn'nnrTG
Cuts at: 0 61 64 681
Size: 61 3 617
Fragments arranged by size:
617 61 3
MspA1I CmG'CkG
Cuts at: 0 141 378 432 681
Size: 141 237 54 249
Fragments arranged by size:
249 237 141 54
NsiI A_TGCA'T
Cuts at: 0 417 681
Size: 417 264
NspI r_CATG'y
Cuts at: 0 666 681
Size: 666 15
PstI C_TGCA'G
Cuts at: 0 560 681
Size: 560 121
PvuII CAG'CTG
Cuts at: 0 378 432 681
Size: 378 54 249
Fragments arranged by size:
378 249 54
SacI G_AGCT'C
Cuts at: 0 457 681
Size: 457 224
SfcI C'TryA_G
Cuts at: 0 465 556 681
Size: 465 91 125
Fragments arranged by size:
465 125 91
SmlI C'TyrA_G
Cuts at: 0 667 681
Size: 667 14
SphI G_CATG'C
Cuts at: 0 666 681
Size: 666 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 290 407 607 640 681
Size: 290 117 200 33 41
Fragments arranged by size:
290 200 117 41 33
Enzymes that do cut:
AcuI AleI AloI AlwNI ApoI BanI BanII BbvCI
BfrBI BmrI BplI BpmI Bpu10I BseRI BsiHKAI BsmI
Bsp1286I BsrBI BstBI BstEII BstXI BtgI EaeI EarI
EciI EcoICRI Eco57MI HincII HpaI MscI MslI MspA1I
NsiI NspI PstI PvuII SacI SfcI SmlI SphI
TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmtI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HindIII KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI