Restriction map for
pFN21AE2621
(Linear) (Six-base) MAPSORT of: KIEE2621.seq Check: 6726 from: 1 to: 2004
KIEE2621.orf
With 182 enzymes: SgfI *
May 17, 2016 15:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 819 2004
Size: 819 1185
Acc65I G'GTAC_C
Cuts at: 0 881 1883 2004
Size: 881 1002 121
Fragments arranged by size:
1002 881 121
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1455 1751 2004
Size: 1455 296 253
Fragments arranged by size:
1455 296 253
AflIII A'CryG_T
Cuts at: 0 425 2004
Size: 425 1579
AgeI A'CCGG_T
Cuts at: 0 1359 2004
Size: 1359 645
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 276 2004
Size: 276 1728
AlwNI CAG_nnn'CTG
Cuts at: 0 158 630 1059 1438 2004
Size: 158 472 429 379 566
Fragments arranged by size:
566 472 429 379 158
ApaI G_GGCC'C
Cuts at: 0 453 1660 1844 2004
Size: 453 1207 184 160
Fragments arranged by size:
1207 453 184 160
ApoI r'AATT_y
Cuts at: 0 1226 2004
Size: 1226 778
AvaI C'yCGr_G
Cuts at: 0 184 690 720 1187 1671 2004
Size: 184 506 30 467 484 333
Fragments arranged by size:
506 484 467 333 184 30
BanI G'GyrC_C
Cuts at: 0 369 391 502 794 881 1883 2004
Size: 369 22 111 292 87 1002 121
Fragments arranged by size:
1002 369 292 121 111 87 22
BanII G_rGCy'C
Cuts at: 0 107 203 453 1004 1660 1844 2004
Size: 107 96 250 551 656 184 160
Fragments arranged by size:
656 551 250 184 160 107 96
BbeI G_GCGC'C
Cuts at: 0 373 395 506 798 2004
Size: 373 22 111 292 1206
Fragments arranged by size:
1206 373 292 111 22
BbvCI CC'TCA_GC
Cuts at: 0 1955 2004
Size: 1955 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1105 1139 2004
Size: 1105 34 865
Fragments arranged by size:
1105 865 34
BglI GCCn_nnn'nGGC
Cuts at: 0 238 361 948 2004
Size: 238 123 587 1056
Fragments arranged by size:
1056 587 238 123
BlpI GC'TnA_GC
Cuts at: 0 1080 2004
Size: 1080 924
Bme1580I G_kGCm'C
Cuts at: 0 453 1660 1844 2004
Size: 453 1207 184 160
Fragments arranged by size:
1207 453 184 160
BmrI ACTGGGnnnn_n'
Cuts at: 0 265 613 2004
Size: 265 348 1391
Fragments arranged by size:
1391 348 265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 297 2004
Size: 297 1707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 550 595 1807 1845 2004
Size: 44 506 45 1212 38 159
Fragments arranged by size:
1212 506 159 45 44 38
Bpu10I CC'TnA_GC
Cuts at: 0 305 1955 2004
Size: 305 1650 49
Fragments arranged by size:
1650 305 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1290 2004
Size: 1290 714
BsaAI yAC'GTr
Cuts at: 0 588 2004
Size: 588 1416
BsaHI Gr'CG_yC
Cuts at: 0 190 370 392 503 581 795 2004
Size: 190 180 22 111 78 214 1209
Fragments arranged by size:
1209 214 190 180 111 78 22
BsaWI w'CCGG_w
Cuts at: 0 129 1359 2004
Size: 129 1230 645
Fragments arranged by size:
1230 645 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 190 220 412 442 1668 1698 1912 1942
Size: 190 30 192 30 1226 30 214 30
Cuts at: 1942 2004
Size: 62
Fragments arranged by size:
1226 214 192 190 62 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1012 1192 1222 1552 1689 1788 1936 2004
Size: 1012 180 30 330 137 99 148 68
Fragments arranged by size:
1012 330 180 148 137 99 68 30
BseYI C'CCAG_C
Cuts at: 0 614 1478 1514 1820 1907 1948 2004
Size: 614 864 36 306 87 41 56
Fragments arranged by size:
864 614 306 87 56 41 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 405 485 615 2004
Size: 113 292 80 130 1389
Fragments arranged by size:
1389 292 130 113 80
BsiEI CG_ry'CG
Cuts at: 0 516 657 2004
Size: 516 141 1347
Fragments arranged by size:
1347 516 141
BsiHKAI G_wGCw'C
Cuts at: 0 107 255 425 778 785 905 1004 1463
Size: 107 148 170 353 7 120 99 459
Cuts at: 1463 1696 2004
Size: 233 308
Fragments arranged by size:
459 353 308 233 170 148 120 107
99 7
Bsp1286I G_dGCh'C
Cuts at: 0 107 203 255 425 453 778 785 905
Size: 107 96 52 170 28 325 7 120
Cuts at: 905 1004 1463 1660 1696 1844 2004
Size: 99 459 197 36 148 160
Fragments arranged by size:
459 325 197 170 160 148 120 107
99 96 52 36 28 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 819 1544 2004
Size: 819 725 460
Fragments arranged by size:
819 725 460
BsrBI CCG'CTC
Cuts at: 0 123 2004
Size: 123 1881
BsrDI GCAATG_nn'
Cuts at: 0 996 2004
Size: 996 1008
BsrFI r'CCGG_y
Cuts at: 0 511 1359 2004
Size: 511 848 645
Fragments arranged by size:
848 645 511
BssHII G'CGCG_C
Cuts at: 0 476 2004
Size: 476 1528
BstAPI GCAn_nnn'nTGC
Cuts at: 0 158 352 648 2004
Size: 158 194 296 1356
Fragments arranged by size:
1356 296 194 158
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1221 2004
Size: 1221 783
Bsu36I CC'TnA_GG
Cuts at: 0 1424 2004
Size: 1424 580
BtgI C'CryG_G
Cuts at: 0 657 1744 2004
Size: 657 1087 260
Fragments arranged by size:
1087 657 260
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 572 2004
Size: 572 1432
BtsI GCAGTG_nn'
Cuts at: 0 96 1629 2004
Size: 96 1533 375
Fragments arranged by size:
1533 375 96
DraIII CAC_nnn'GTG
Cuts at: 0 591 866 2004
Size: 591 275 1138
Fragments arranged by size:
1138 591 275
EaeI y'GGCC_r
Cuts at: 0 362 513 654 747 1811 2004
Size: 362 151 141 93 1064 193
Fragments arranged by size:
1064 362 193 151 141 93
EagI C'GGCC_G
Cuts at: 0 513 654 2004
Size: 513 141 1350
Fragments arranged by size:
1350 513 141
EarI CTCTTCn'nnn_
Cuts at: 0 13 1583 1704 2004
Size: 13 1570 121 300
Fragments arranged by size:
1570 300 121 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 286 2004
Size: 286 1718
EcoICRI GAG'CTC
Cuts at: 0 105 1002 2004
Size: 105 897 1002
Fragments arranged by size:
1002 897 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 550 595 1455 1751 1807 1845 2004
Size: 44 506 45 860 296 56 38 159
Fragments arranged by size:
860 506 296 159 56 45 44 38
EcoO109I rG'GnC_Cy
Cuts at: 0 570 627 1071 1413 1657 1840 1841 1943
Size: 570 57 444 342 244 183 1 102
Cuts at: 1943 2004
Size: 61
Fragments arranged by size:
570 444 342 244 183 102 61 57
1
EcoRI G'AATT_C
Cuts at: 0 1226 2004
Size: 1226 778
HaeII r_GCGC'y
Cuts at: 0 217 373 395 506 798 812 1478 2004
Size: 217 156 22 111 292 14 666 526
Fragments arranged by size:
666 526 292 217 156 111 22 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 772 804 1420 1426 1452 1458
Size: 532 32 208 32 616 6 26 6
Cuts at: 1458 1633 1665 1843 1875 2004
Size: 175 32 178 32 129
Fragments arranged by size:
616 532 208 178 175 129 32 32
32 32 26 6 6
HindIII A'AGCT_T
Cuts at: 0 701 2004
Size: 701 1303
KasI G'GCGC_C
Cuts at: 0 369 391 502 794 2004
Size: 369 22 111 292 1210
Fragments arranged by size:
1210 369 292 111 22
KpnI G_GTAC'C
Cuts at: 0 885 1887 2004
Size: 885 1002 117
Fragments arranged by size:
1002 885 117
MluI A'CGCG_T
Cuts at: 0 425 2004
Size: 425 1579
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1037 1064 2004
Size: 1037 27 940
Fragments arranged by size:
1037 940 27
MscI TGG'CCA
Cuts at: 0 1813 2004
Size: 1813 191
MspA1I CmG'CkG
Cuts at: 0 158 164 389 659 818 1518 2004
Size: 158 6 225 270 159 700 486
Fragments arranged by size:
700 486 270 225 159 158 6
NaeI GCC'GGC
Cuts at: 0 513 2004
Size: 513 1491
NarI GG'CG_CC
Cuts at: 0 370 392 503 795 2004
Size: 370 22 111 292 1209
Fragments arranged by size:
1209 370 292 111 22
NcoI C'CATG_G
Cuts at: 0 1744 2004
Size: 1744 260
NgoMIV G'CCGG_C
Cuts at: 0 511 2004
Size: 511 1493
NspI r_CATG'y
Cuts at: 0 1600 2004
Size: 1600 404
PasI CC'CwG_GG
Cuts at: 0 1653 2004
Size: 1653 351
PflMI CCAn_nnn'nTGG
Cuts at: 0 915 1059 2004
Size: 915 144 945
Fragments arranged by size:
945 915 144
PfoI T'CCnGG_A
Cuts at: 0 1087 1784 2004
Size: 1087 697 220
Fragments arranged by size:
1087 697 220
PmlI CAC'GTG
Cuts at: 0 588 2004
Size: 588 1416
PpuMI rG'GwC_Cy
Cuts at: 0 570 1071 1943 2004
Size: 570 501 872 61
Fragments arranged by size:
872 570 501 61
PspOMI G'GGCC_C
Cuts at: 0 449 1656 1840 2004
Size: 449 1207 184 164
Fragments arranged by size:
1207 449 184 164
PspXI vC'TCGA_Gb
Cuts at: 0 184 2004
Size: 184 1820
PstI C_TGCA'G
Cuts at: 0 163 327 1051 2004
Size: 163 164 724 953
Fragments arranged by size:
953 724 164 163
PvuII CAG'CTG
Cuts at: 0 158 2004
Size: 158 1846
RsrII CG'GwC_CG
Cuts at: 0 132 2004
Size: 132 1872
SacI G_AGCT'C
Cuts at: 0 107 1004 2004
Size: 107 897 1000
Fragments arranged by size:
1000 897 107
SacII CC_GC'GG
Cuts at: 0 660 2004
Size: 660 1344
SanDI GG'GwC_CC
Cuts at: 0 1943 2004
Size: 1943 61
SapI GCTCTTCn'nnn_
Cuts at: 0 13 2004
Size: 13 1991
SexAI A'CCwGG_T
Cuts at: 0 677 2004
Size: 677 1327
SfcI C'TryA_G
Cuts at: 0 159 323 1047 1145 2004
Size: 159 164 724 98 859
Fragments arranged by size:
859 724 164 159 98
SfoI GGC'GCC
Cuts at: 0 371 393 504 796 2004
Size: 371 22 111 292 1208
Fragments arranged by size:
1208 371 292 111 22
SmaI CCC'GGG
Cuts at: 0 722 2004
Size: 722 1282
SmlI C'TyrA_G
Cuts at: 0 184 1269 2004
Size: 184 1085 735
Fragments arranged by size:
1085 735 184
SphI G_CATG'C
Cuts at: 0 1600 2004
Size: 1600 404
StyI C'CwwG_G
Cuts at: 0 896 914 944 1236 1571 1744 2004
Size: 896 18 30 292 335 173 260
Fragments arranged by size:
896 335 292 260 173 30 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1834 2004
Size: 1834 170
TatI w'GTAC_w
Cuts at: 0 977 2004
Size: 977 1027
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 190 222 412 444 949 981 1090 1122
Size: 190 32 190 32 505 32 109 32
Cuts at: 1122 1668 1700 2004
Size: 546 32 304
Fragments arranged by size:
546 505 304 190 190 109 32 32
32 32 32
XhoI C'TCGA_G
Cuts at: 0 184 2004
Size: 184 1820
XmaI C'CCGG_G
Cuts at: 0 720 2004
Size: 720 1284
Enzymes that do cut:
AarI Acc65I AcuI AflIII AgeI AlfI AlwNI ApaI
ApoI AvaI BanI BanII BbeI BbvCI BcgI BglI
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstXI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI HaeII Hin4I HindIII
KasI KpnI MluI MmeI MscI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpuMI
PspOMI PspXI PstI PvuII RsrII SacI SacII SanDI
SapI SexAI SfcI SfoI SmaI SmlI SphI StyI
TaqII TatI TstI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AhdI AleI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BciVI BclI BfrBI BglII BmgBI BmtI BsaI BsaBI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BssSI BstBI
BstEII BstYI BstZ17I ClaI CspCI DraI DrdI EcoNI
EcoRV FalI FseI FspI FspAI HincII HpaI MfeI
MslI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PpiI PshAI PsiI PsrI PvuI SalI SbfI
ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI
StuI SwaI Tth111I XbaI XcmI XmnI ZraI