Restriction map for
pFN21AE2614
(Linear) (Six-base) MAPSORT of: KIEE2614.seq Check: 6794 from: 1 to: 1590
KIEE2614.orf
With 182 enzymes: SgfI *
March 25, 2016 09:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1256 1590
Size: 1256 334
AatII G_ACGT'C
Cuts at: 0 875 1590
Size: 875 715
AccI GT'mk_AC
Cuts at: 0 1304 1590
Size: 1304 286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 999 1590
Size: 999 591
AflIII A'CryG_T
Cuts at: 0 461 1472 1590
Size: 461 1011 118
Fragments arranged by size:
1011 461 118
AleI CACnn'nnGTG
Cuts at: 0 271 1590
Size: 271 1319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1264 1564 1590
Size: 1264 300 26
Fragments arranged by size:
1264 300 26
AlwNI CAG_nnn'CTG
Cuts at: 0 455 1590
Size: 455 1135
ApaI G_GGCC'C
Cuts at: 0 436 1590
Size: 436 1154
ApaLI G'TGCA_C
Cuts at: 0 847 1590
Size: 847 743
AvaI C'yCGr_G
Cuts at: 0 57 482 1120 1590
Size: 57 425 638 470
Fragments arranged by size:
638 470 425 57
BanI G'GyrC_C
Cuts at: 0 520 1240 1590
Size: 520 720 350
Fragments arranged by size:
720 520 350
BanII G_rGCy'C
Cuts at: 0 7 35 436 1023 1429 1590
Size: 7 28 401 587 406 161
Fragments arranged by size:
587 406 401 161 28 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 1269 1590
Size: 1269 321
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 648 682 750 784 1590
Size: 648 34 68 34 806
Fragments arranged by size:
806 648 68 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 156 1590
Size: 156 1434
BglI GCCn_nnn'nGGC
Cuts at: 0 809 1590
Size: 809 781
Bme1580I G_kGCm'C
Cuts at: 0 188 436 523 851 1243 1400 1590
Size: 188 248 87 328 392 157 190
Fragments arranged by size:
392 328 248 190 188 157 87
BmgBI CAC'GTC
Cuts at: 0 273 462 1590
Size: 273 189 1128
Fragments arranged by size:
1128 273 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 742 1590
Size: 118 624 848
Fragments arranged by size:
848 624 118
Bpu10I CC'TnA_GC
Cuts at: 0 119 1590
Size: 119 1471
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 1590
Size: 46 1544
BsaAI yAC'GTr
Cuts at: 0 1041 1590
Size: 1041 549
BsaHI Gr'CG_yC
Cuts at: 0 146 872 1590
Size: 146 726 718
Fragments arranged by size:
726 718 146
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 715 745 1057 1087 1590
Size: 715 30 312 30 503
Fragments arranged by size:
715 503 312 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 115 1590
Size: 43 72 1475
Fragments arranged by size:
1475 72 43
BseYI C'CCAG_C
Cuts at: 0 34 150 888 930 1309 1488 1590
Size: 34 116 738 42 379 179 102
Fragments arranged by size:
738 379 179 116 102 42 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 522 565 727 1015 1494 1534 1590
Size: 379 143 43 162 288 479 40 56
Fragments arranged by size:
479 379 288 162 143 56 43 40
BsiEI CG_ry'CG
Cuts at: 0 793 1590
Size: 793 797
BsiHKAI G_wGCw'C
Cuts at: 0 622 776 851 887 1590
Size: 622 154 75 36 703
Fragments arranged by size:
703 622 154 75 36
Bsp1286I G_dGCh'C
Cuts at: 0 7 35 188 436 523 622 776 851
Size: 7 28 153 248 87 99 154 75
Cuts at: 851 887 1023 1243 1400 1429 1590
Size: 36 136 220 157 29 161
Fragments arranged by size:
248 220 161 157 154 153 136 99
87 75 36 29 28 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 290 1256 1590
Size: 290 966 334
Fragments arranged by size:
966 334 290
BsrFI r'CCGG_y
Cuts at: 0 946 1590
Size: 946 644
BssHII G'CGCG_C
Cuts at: 0 681 1590
Size: 681 909
BssSI C'ACGA_G
Cuts at: 0 937 1590
Size: 937 653
BstEII G'GTnAC_C
Cuts at: 0 942 1590
Size: 942 648
BstXI CCAn_nnnn'nTGG
Cuts at: 0 507 1407 1590
Size: 507 900 183
Fragments arranged by size:
900 507 183
BstYI r'GATC_y
Cuts at: 0 1130 1208 1590
Size: 1130 78 382
Fragments arranged by size:
1130 382 78
Bsu36I CC'TnA_GG
Cuts at: 0 133 1590
Size: 133 1457
BtgI C'CryG_G
Cuts at: 0 8 524 982 1226 1499 1590
Size: 8 516 458 244 273 91
Fragments arranged by size:
516 458 273 244 91 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 232 1062 1590
Size: 232 830 528
Fragments arranged by size:
830 528 232
BtsI GCAGTG_nn'
Cuts at: 0 1137 1357 1590
Size: 1137 220 233
Fragments arranged by size:
1137 233 220
DraIII CAC_nnn'GTG
Cuts at: 0 942 1103 1590
Size: 942 161 487
Fragments arranged by size:
942 487 161
DrdI GACnn_nn'nnGTC
Cuts at: 0 1491 1590
Size: 1491 99
EaeI y'GGCC_r
Cuts at: 0 509 790 985 1590
Size: 509 281 195 605
Fragments arranged by size:
605 509 281 195
EagI C'GGCC_G
Cuts at: 0 790 1590
Size: 790 800
EarI CTCTTCn'nnn_
Cuts at: 0 481 781 1294 1590
Size: 481 300 513 296
Fragments arranged by size:
513 481 300 296
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 124 1123 1485 1590
Size: 124 999 362 105
Fragments arranged by size:
999 362 124 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 742 999 1590
Size: 118 624 257 591
Fragments arranged by size:
624 591 257 118
EcoO109I rG'GnC_Cy
Cuts at: 0 129 302 433 959 1590
Size: 129 173 131 526 631
Fragments arranged by size:
631 526 173 131 129
HaeII r_GCGC'y
Cuts at: 0 24 721 1590
Size: 24 697 869
Fragments arranged by size:
869 697 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 624 656 1057 1089 1590
Size: 624 32 401 32 501
Fragments arranged by size:
624 501 401 32 32
MslI CAynn'nnrTG
Cuts at: 0 271 340 574 1081 1177 1462 1590
Size: 271 69 234 507 96 285 128
Fragments arranged by size:
507 285 271 234 128 96 69
MspA1I CmG'CkG
Cuts at: 0 96 355 514 953 984 1004 1313 1437
Size: 96 259 159 439 31 20 309 124
Cuts at: 1437 1544 1590
Size: 107 46
Fragments arranged by size:
439 309 259 159 124 107 96 46
31 20
NspI r_CATG'y
Cuts at: 0 969 1071 1320 1476 1590
Size: 969 102 249 156 114
Fragments arranged by size:
969 249 156 114 102
PasI CC'CwG_GG
Cuts at: 0 298 1590
Size: 298 1292
PciI A'CATG_T
Cuts at: 0 1472 1590
Size: 1472 118
PflMI CCAn_nnn'nTGG
Cuts at: 0 1251 1590
Size: 1251 339
PpuMI rG'GwC_Cy
Cuts at: 0 302 1590
Size: 302 1288
PspOMI G'GGCC_C
Cuts at: 0 432 1590
Size: 432 1158
PspXI vC'TCGA_Gb
Cuts at: 0 1120 1590
Size: 1120 470
PstI C_TGCA'G
Cuts at: 0 473 1590
Size: 473 1117
PvuII CAG'CTG
Cuts at: 0 96 355 1004 1544 1590
Size: 96 259 649 540 46
Fragments arranged by size:
649 540 259 96 46
SacII CC_GC'GG
Cuts at: 0 985 1590
Size: 985 605
SapI GCTCTTCn'nnn_
Cuts at: 0 481 781 1590
Size: 481 300 809
Fragments arranged by size:
809 481 300
SfcI C'TryA_G
Cuts at: 0 469 1515 1590
Size: 469 1046 75
Fragments arranged by size:
1046 469 75
SmaI CCC'GGG
Cuts at: 0 59 1590
Size: 59 1531
SmlI C'TyrA_G
Cuts at: 0 25 1120 1590
Size: 25 1095 470
Fragments arranged by size:
1095 470 25
SphI G_CATG'C
Cuts at: 0 1320 1590
Size: 1320 270
StuI AGG'CCT
Cuts at: 0 493 821 1586 1590
Size: 493 328 765 4
Fragments arranged by size:
765 493 328 4
StyI C'CwwG_G
Cuts at: 0 428 988 1590
Size: 428 560 602
Fragments arranged by size:
602 560 428
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 902 1258 1590
Size: 902 356 332
Fragments arranged by size:
902 356 332
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 715 747 1590
Size: 715 32 843
Fragments arranged by size:
843 715 32
Tth111I GACn'n_nGTC
Cuts at: 0 859 1464 1590
Size: 859 605 126
Fragments arranged by size:
859 605 126
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1170 1590
Size: 1170 420
XhoI C'TCGA_G
Cuts at: 0 1120 1590
Size: 1120 470
XmaI C'CCGG_G
Cuts at: 0 57 1590
Size: 57 1533
ZraI GAC'GTC
Cuts at: 0 873 1590
Size: 873 717
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI AvaI BanI BanII BbsI BcgI BciVI
BglI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrFI BssHII BssSI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I HaeII Hin4I MslI MspA1I NspI PasI
PciI PflMI PpuMI PspOMI PspXI PstI PvuII SacII
SapI SfcI SmaI SmlI SphI StuI StyI TaqII
TstI Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BclI BfrBI BglII BlpI BmrI BmtI BplI BsaI
BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BstAPI BstBI BstZ17I ClaI CspCI DraI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PfoI PmeI PmlI PpiI PshAI
PsiI PsrI PvuI RsrII SacI SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI TatI XbaI XmnI