Restriction map for
pFN21AE2613
(Linear) (Six-base) MAPSORT of: KIEE2613.seq Check: 5456 from: 1 to: 1230
KIEE2613.orf
With 182 enzymes: SgfI *
March 25, 2016 09:08 ..
Acc65I G'GTAC_C
Cuts at: 0 1100 1230
Size: 1100 130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 928 1230
Size: 928 302
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 52 84 1230
Size: 52 32 1146
Fragments arranged by size:
1146 52 32
AlwNI CAG_nnn'CTG
Cuts at: 0 90 681 1097 1230
Size: 90 591 416 133
Fragments arranged by size:
591 416 133 90
AvaI C'yCGr_G
Cuts at: 0 257 533 1230
Size: 257 276 697
Fragments arranged by size:
697 276 257
BamHI G'GATC_C
Cuts at: 0 101 1230
Size: 101 1129
BanI G'GyrC_C
Cuts at: 0 34 320 1100 1143 1230
Size: 34 286 780 43 87
Fragments arranged by size:
780 286 87 43 34
BanII G_rGCy'C
Cuts at: 0 17 161 667 839 1141 1230
Size: 17 144 506 172 302 89
Fragments arranged by size:
506 302 172 144 89 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 420 925 1230
Size: 420 505 305
Fragments arranged by size:
505 420 305
BbvCI CC'TCA_GC
Cuts at: 0 1160 1166 1230
Size: 1160 6 64
Fragments arranged by size:
1160 64 6
BfrBI ATG'CAT
Cuts at: 0 269 1230
Size: 269 961
BglI GCCn_nnn'nGGC
Cuts at: 0 648 899 1230
Size: 648 251 331
Fragments arranged by size:
648 331 251
BglII A'GATC_T
Cuts at: 0 539 1230
Size: 539 691
BlpI GC'TnA_GC
Cuts at: 0 553 578 1230
Size: 553 25 652
Fragments arranged by size:
652 553 25
Bme1580I G_kGCm'C
Cuts at: 0 99 470 1148 1230
Size: 99 371 678 82
Fragments arranged by size:
678 371 99 82
BmrI ACTGGGnnnn_n'
Cuts at: 0 141 1230
Size: 141 1089
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 177 1230
Size: 177 1053
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 172 181 214 655 853 870 1009
Size: 147 25 9 33 441 198 17 139
Cuts at: 1009 1230
Size: 221
Fragments arranged by size:
441 221 198 147 139 33 25 17
9
Bpu10I CC'TnA_GC
Cuts at: 0 1160 1166 1230
Size: 1160 6 64
Fragments arranged by size:
1160 64 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1192 1230
Size: 1192 38
BsaAI yAC'GTr
Cuts at: 0 243 1230
Size: 243 987
BsaBI GATnn'nnATC
Cuts at: 0 515 1230
Size: 515 715
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 430 433 436 941 974 1230
Size: 169 261 3 3 505 33 256
Fragments arranged by size:
505 261 256 169 33 3 3
BseYI C'CCAG_C
Cuts at: 0 125 211 451 1230
Size: 125 86 240 779
Fragments arranged by size:
779 240 125 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 765 1230
Size: 765 465
BsiHKAI G_wGCw'C
Cuts at: 0 161 1230
Size: 161 1069
BsmBI CGTCTCn'nnnn_
Cuts at: 0 875 1230
Size: 875 355
Bsp1286I G_dGCh'C
Cuts at: 0 17 99 161 470 667 839 1141 1148
Size: 17 82 62 309 197 172 302 7
Cuts at: 1148 1230
Size: 82
Fragments arranged by size:
309 302 197 172 82 82 62 17
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1051 1230
Size: 1051 179
BsrDI GCAATG_nn'
Cuts at: 0 179 958 1177 1230
Size: 179 779 219 53
Fragments arranged by size:
779 219 179 53
BsrFI r'CCGG_y
Cuts at: 0 49 1230
Size: 49 1181
BsrGI T'GTAC_A
Cuts at: 0 302 1230
Size: 302 928
BstAPI GCAn_nnn'nTGC
Cuts at: 0 181 1230
Size: 181 1049
BstXI CCAn_nnnn'nTGG
Cuts at: 0 171 384 894 1111 1230
Size: 171 213 510 217 119
Fragments arranged by size:
510 217 213 171 119
BstYI r'GATC_y
Cuts at: 0 101 539 1230
Size: 101 438 691
Fragments arranged by size:
691 438 101
BtgI C'CryG_G
Cuts at: 0 506 1230
Size: 506 724
BtsI GCAGTG_nn'
Cuts at: 0 729 1079 1230
Size: 729 350 151
Fragments arranged by size:
729 350 151
DrdI GACnn_nn'nnGTC
Cuts at: 0 184 1230
Size: 184 1046
EaeI y'GGCC_r
Cuts at: 0 386 461 749 1230
Size: 386 75 288 481
Fragments arranged by size:
481 386 288 75
EarI CTCTTCn'nnn_
Cuts at: 0 998 1230
Size: 998 232
EcoICRI GAG'CTC
Cuts at: 0 159 1230
Size: 159 1071
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 172 181 214 655 853 870 1009
Size: 147 25 9 33 441 198 17 139
Cuts at: 1009 1230
Size: 221
Fragments arranged by size:
441 221 198 147 139 33 25 17
9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 486 1230
Size: 486 744
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 279 311 1230
Size: 279 32 919
Fragments arranged by size:
919 279 32
KpnI G_GTAC'C
Cuts at: 0 1104 1230
Size: 1104 126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 1230
Size: 542 688
MscI TGG'CCA
Cuts at: 0 388 1230
Size: 388 842
MslI CAynn'nnrTG
Cuts at: 0 382 1109 1230
Size: 382 727 121
Fragments arranged by size:
727 382 121
MspA1I CmG'CkG
Cuts at: 0 678 890 1230
Size: 678 212 340
Fragments arranged by size:
678 340 212
NaeI GCC'GGC
Cuts at: 0 51 1230
Size: 51 1179
NcoI C'CATG_G
Cuts at: 0 506 1230
Size: 506 724
NdeI CA'TA_TG
Cuts at: 0 714 1230
Size: 714 516
NgoMIV G'CCGG_C
Cuts at: 0 49 1230
Size: 49 1181
NsiI A_TGCA'T
Cuts at: 0 271 1230
Size: 271 959
NspI r_CATG'y
Cuts at: 0 269 349 1230
Size: 269 80 881
Fragments arranged by size:
881 269 80
PasI CC'CwG_GG
Cuts at: 0 660 1230
Size: 660 570
PflMI CCAn_nnn'nTGG
Cuts at: 0 512 681 1097 1230
Size: 512 169 416 133
Fragments arranged by size:
512 416 169 133
PfoI T'CCnGG_A
Cuts at: 0 56 830 1230
Size: 56 774 400
Fragments arranged by size:
774 400 56
PvuII CAG'CTG
Cuts at: 0 890 1230
Size: 890 340
SacI G_AGCT'C
Cuts at: 0 161 1230
Size: 161 1069
SfcI C'TryA_G
Cuts at: 0 144 1230
Size: 144 1086
SmaI CCC'GGG
Cuts at: 0 259 1230
Size: 259 971
SmlI C'TyrA_G
Cuts at: 0 1207 1230
Size: 1207 23
SnaBI TAC'GTA
Cuts at: 0 243 1230
Size: 243 987
SphI G_CATG'C
Cuts at: 0 269 1230
Size: 269 961
StyI C'CwwG_G
Cuts at: 0 482 506 807 1132 1230
Size: 482 24 301 325 98
Fragments arranged by size:
482 325 301 98 24
TatI w'GTAC_w
Cuts at: 0 302 1230
Size: 302 928
XmaI C'CCGG_G
Cuts at: 0 257 1230
Size: 257 973
Enzymes that do cut:
Acc65I AlfI AloI AlwNI AvaI BamHI BanI BanII
BbsI BbvCI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI
BstAPI BstXI BstYI BtgI BtsI DrdI EaeI EarI
EcoICRI Eco57MI EcoNI Hin4I KpnI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PasI
PflMI PfoI PvuII SacI SfcI SmaI SmlI SnaBI
SphI StyI TatI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BcgI BciVI BclI BmgBI
BmtI BsaI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BspEI BspHI BsrBI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EciI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI MfeI MluI NarI NheI
NotI NruI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SpeI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmnI ZraI