Restriction map for
pFN21AE2608
(Linear) (Six-base) MAPSORT of: KIEE2608.seq Check: 4411 from: 1 to: 1047
KIEE2608.orf
With 182 enzymes: SgfI *
February 23, 2016 17:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 600 1047
Size: 600 447
AfeI AGC'GCT
Cuts at: 0 143 1047
Size: 143 904
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 320 1047
Size: 320 727
AlwNI CAG_nnn'CTG
Cuts at: 0 277 516 592 1047
Size: 277 239 76 455
Fragments arranged by size:
455 277 239 76
ApaI G_GGCC'C
Cuts at: 0 184 205 1047
Size: 184 21 842
Fragments arranged by size:
842 184 21
ApoI r'AATT_y
Cuts at: 0 955 1047
Size: 955 92
AvaI C'yCGr_G
Cuts at: 0 183 358 1006 1047
Size: 183 175 648 41
Fragments arranged by size:
648 183 175 41
BamHI G'GATC_C
Cuts at: 0 1030 1047
Size: 1030 17
BanI G'GyrC_C
Cuts at: 0 76 552 1047
Size: 76 476 495
Fragments arranged by size:
495 476 76
BanII G_rGCy'C
Cuts at: 0 184 205 500 1007 1047
Size: 184 21 295 507 40
Fragments arranged by size:
507 295 184 40 21
BbeI G_GCGC'C
Cuts at: 0 80 556 1047
Size: 80 476 491
Fragments arranged by size:
491 476 80
BbsI GAAGACnn'nnnn_
Cuts at: 0 722 1047
Size: 722 325
BbvCI CC'TCA_GC
Cuts at: 0 278 1047
Size: 278 769
BglI GCCn_nnn'nGGC
Cuts at: 0 74 179 1047
Size: 74 105 868
Fragments arranged by size:
868 105 74
BlpI GC'TnA_GC
Cuts at: 0 64 1047
Size: 64 983
Bme1580I G_kGCm'C
Cuts at: 0 184 205 1047
Size: 184 21 842
Fragments arranged by size:
842 184 21
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 555 1047
Size: 555 492
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 246 362 762 1047
Size: 79 167 116 400 285
Fragments arranged by size:
400 285 167 116 79
Bpu10I CC'TnA_GC
Cuts at: 0 278 293 1047
Size: 278 15 754
Fragments arranged by size:
754 278 15
BsaHI Gr'CG_yC
Cuts at: 0 77 151 553 1047
Size: 77 74 402 494
Fragments arranged by size:
494 402 77 74
BsaWI w'CCGG_w
Cuts at: 0 962 1047
Size: 962 85
BseYI C'CCAG_C
Cuts at: 0 4 138 214 472 638 1047
Size: 4 134 76 258 166 409
Fragments arranged by size:
409 258 166 134 76 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 861 1047
Size: 427 434 186
Fragments arranged by size:
434 427 186
BsiHKAI G_wGCw'C
Cuts at: 0 416 500 1047
Size: 416 84 547
Fragments arranged by size:
547 416 84
BsmBI CGTCTCn'nnnn_
Cuts at: 0 414 1003 1047
Size: 414 589 44
Fragments arranged by size:
589 414 44
Bsp1286I G_dGCh'C
Cuts at: 0 184 205 416 500 1007 1047
Size: 184 21 211 84 507 40
Fragments arranged by size:
507 211 184 84 40 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 338 600 869 1047
Size: 338 262 269 178
Fragments arranged by size:
338 269 262 178
BsrBI CCG'CTC
Cuts at: 0 25 1047
Size: 25 1022
BsrDI GCAATG_nn'
Cuts at: 0 346 1047
Size: 346 701
BsrFI r'CCGG_y
Cuts at: 0 173 1047
Size: 173 874
BstAPI GCAn_nnn'nTGC
Cuts at: 0 592 1047
Size: 592 455
BstEII G'GTnAC_C
Cuts at: 0 240 1047
Size: 240 807
BstXI CCAn_nnnn'nTGG
Cuts at: 0 146 549 1047
Size: 146 403 498
Fragments arranged by size:
498 403 146
BstYI r'GATC_y
Cuts at: 0 1030 1047
Size: 1030 17
Bsu36I CC'TnA_GG
Cuts at: 0 155 1047
Size: 155 892
BtgI C'CryG_G
Cuts at: 0 527 765 1047
Size: 527 238 282
Fragments arranged by size:
527 282 238
EaeI y'GGCC_r
Cuts at: 0 1013 1047
Size: 1013 34
EarI CTCTTCn'nnn_
Cuts at: 0 596 1047
Size: 596 451
EcoICRI GAG'CTC
Cuts at: 0 498 1047
Size: 498 549
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 246 362 762 1047
Size: 79 167 116 400 285
Fragments arranged by size:
400 285 167 116 79
EcoNI CCTnn'n_nnAGG
Cuts at: 0 316 1047
Size: 316 731
EcoO109I rG'GnC_Cy
Cuts at: 0 92 180 201 327 469 1047
Size: 92 88 21 126 142 578
Fragments arranged by size:
578 142 126 92 88 21
HaeII r_GCGC'y
Cuts at: 0 80 145 556 996 1047
Size: 80 65 411 440 51
Fragments arranged by size:
440 411 80 65 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 446 478 1015 1022 1047
Size: 446 32 537 7 25
Fragments arranged by size:
537 446 32 25 7
KasI G'GCGC_C
Cuts at: 0 76 552 1047
Size: 76 476 495
Fragments arranged by size:
495 476 76
MslI CAynn'nnrTG
Cuts at: 0 928 1047
Size: 928 119
MspA1I CmG'CkG
Cuts at: 0 767 1047
Size: 767 280
NaeI GCC'GGC
Cuts at: 0 175 1047
Size: 175 872
NarI GG'CG_CC
Cuts at: 0 77 553 1047
Size: 77 476 494
Fragments arranged by size:
494 476 77
NcoI C'CATG_G
Cuts at: 0 527 1047
Size: 527 520
NgoMIV G'CCGG_C
Cuts at: 0 173 1047
Size: 173 874
NspI r_CATG'y
Cuts at: 0 927 1047
Size: 927 120
PflMI CCAn_nnn'nTGG
Cuts at: 0 516 1047
Size: 516 531
PpuMI rG'GwC_Cy
Cuts at: 0 92 327 469 1047
Size: 92 235 142 578
Fragments arranged by size:
578 235 142 92
PspOMI G'GGCC_C
Cuts at: 0 180 201 1047
Size: 180 21 846
Fragments arranged by size:
846 180 21
PstI C_TGCA'G
Cuts at: 0 309 320 1047
Size: 309 11 727
Fragments arranged by size:
727 309 11
SacI G_AGCT'C
Cuts at: 0 500 1047
Size: 500 547
SacII CC_GC'GG
Cuts at: 0 768 1047
Size: 768 279
SanDI GG'GwC_CC
Cuts at: 0 92 1047
Size: 92 955
SbfI CC_TGCA'GG
Cuts at: 0 309 1047
Size: 309 738
SfcI C'TryA_G
Cuts at: 0 305 316 1047
Size: 305 11 731
Fragments arranged by size:
731 305 11
SfoI GGC'GCC
Cuts at: 0 78 554 1047
Size: 78 476 493
Fragments arranged by size:
493 476 78
SgrAI Cr'CCGG_yG
Cuts at: 0 173 1047
Size: 173 874
SmaI CCC'GGG
Cuts at: 0 185 1047
Size: 185 862
SrfI GCCC'GGGC
Cuts at: 0 185 1047
Size: 185 862
StuI AGG'CCT
Cuts at: 0 209 259 311 896 1047
Size: 209 50 52 585 151
Fragments arranged by size:
585 209 151 52 50
StyI C'CwwG_G
Cuts at: 0 447 458 527 1047
Size: 447 11 69 520
Fragments arranged by size:
520 447 69 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 721 1047
Size: 721 326
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 226 684 1047
Size: 226 458 363
Fragments arranged by size:
458 363 226
XmaI C'CCGG_G
Cuts at: 0 183 1047
Size: 183 864
Enzymes that do cut:
AarI AfeI AlfI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I
BplI BpmI Bpu10I BsaHI BsaWI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstEII
BstXI BstYI Bsu36I BtgI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PflMI PpuMI PspOMI PstI
SacI SacII SanDI SbfI SfcI SfoI SgrAI SmaI
SrfI StuI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BcgI BciVI BclI BfrBI BglII BmgBI BmrI
BmtI BpuEI BsaI BsaAI BsaBI BsaXI BseRI BsiEI
BsiWI BsmI BspEI BspHI BsrGI BssHII BssSI BstBI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SalI SapI ScaI SexAI SfiI
SgfI SmlI SnaBI SpeI SphI SspI SwaI TatI
TstI Tth111I XbaI XhoI XmnI ZraI