Restriction map for
pFN21AE2599
(Linear) (Six-base) MAPSORT of: KIEE2599.seq Check: 6015 from: 1 to: 1218
KIEE2599.orf
With 182 enzymes: SgfI *
January 6, 2016 09:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 15 1218
Size: 15 1203
Acc65I G'GTAC_C
Cuts at: 0 392 1218
Size: 392 826
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 796 1070 1174 1218
Size: 106 690 274 104 44
Fragments arranged by size:
690 274 106 104 44
AfeI AGC'GCT
Cuts at: 0 904 1218
Size: 904 314
AflIII A'CryG_T
Cuts at: 0 336 1218
Size: 336 882
AhdI GACnn_n'nnGTC
Cuts at: 0 1096 1218
Size: 1096 122
AleI CACnn'nnGTG
Cuts at: 0 484 1218
Size: 484 734
AlwNI CAG_nnn'CTG
Cuts at: 0 1143 1218
Size: 1143 75
AvaI C'yCGr_G
Cuts at: 0 171 1218
Size: 171 1047
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 384 417 1218
Size: 384 33 801
Fragments arranged by size:
801 384 33
BanI G'GyrC_C
Cuts at: 0 3 392 448 853 1218
Size: 3 389 56 405 365
Fragments arranged by size:
405 389 365 56 3
BanII G_rGCy'C
Cuts at: 0 287 662 1218
Size: 287 375 556
Fragments arranged by size:
556 375 287
BbeI G_GCGC'C
Cuts at: 0 452 857 1218
Size: 452 405 361
Fragments arranged by size:
452 405 361
BglI GCCn_nnn'nGGC
Cuts at: 0 96 1218
Size: 96 1122
Bme1580I G_kGCm'C
Cuts at: 0 481 606 1218
Size: 481 125 612
Fragments arranged by size:
612 481 125
BmgBI CAC'GTC
Cuts at: 0 339 786 1218
Size: 339 447 432
Fragments arranged by size:
447 432 339
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 1003 1153 1218
Size: 676 327 150 65
Fragments arranged by size:
676 327 150 65
Bpu10I CC'TnA_GC
Cuts at: 0 944 1210 1218
Size: 944 266 8
Fragments arranged by size:
944 266 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 941 1218
Size: 646 295 277
Fragments arranged by size:
646 295 277
BsaHI Gr'CG_yC
Cuts at: 0 449 854 1169 1218
Size: 449 405 315 49
Fragments arranged by size:
449 405 315 49
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 488 518 1218
Size: 488 30 700
Fragments arranged by size:
700 488 30
BseYI C'CCAG_C
Cuts at: 0 91 259 549 1070 1188 1218
Size: 91 168 290 521 118 30
Fragments arranged by size:
521 290 168 118 91 30
BsiEI CG_ry'CG
Cuts at: 0 137 141 1218
Size: 137 4 1077
Fragments arranged by size:
1077 137 4
BsiHKAI G_wGCw'C
Cuts at: 0 662 1218
Size: 662 556
Bsp1286I G_dGCh'C
Cuts at: 0 287 481 606 662 1218
Size: 287 194 125 56 556
Fragments arranged by size:
556 287 194 125 56
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 15 1218
Size: 15 1203
BsrBI CCG'CTC
Cuts at: 0 1209 1218
Size: 1209 9
BsrDI GCAATG_nn'
Cuts at: 0 539 1218
Size: 539 679
BsrFI r'CCGG_y
Cuts at: 0 95 1120 1218
Size: 95 1025 98
Fragments arranged by size:
1025 98 95
BstYI r'GATC_y
Cuts at: 0 930 1218
Size: 930 288
Bsu36I CC'TnA_GG
Cuts at: 0 889 1218
Size: 889 329
BtgI C'CryG_G
Cuts at: 0 473 749 949 1218
Size: 473 276 200 269
Fragments arranged by size:
473 276 269 200
BtsI GCAGTG_nn'
Cuts at: 0 450 715 830 1218
Size: 450 265 115 388
Fragments arranged by size:
450 388 265 115
DraI TTT'AAA
Cuts at: 0 971 1218
Size: 971 247
DrdI GACnn_nn'nnGTC
Cuts at: 0 361 1218
Size: 361 857
EaeI y'GGCC_r
Cuts at: 0 138 509 587 827 1218
Size: 138 371 78 240 391
Fragments arranged by size:
391 371 240 138 78
EagI C'GGCC_G
Cuts at: 0 138 1218
Size: 138 1080
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 870 1218
Size: 870 348
EcoICRI GAG'CTC
Cuts at: 0 660 1218
Size: 660 558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 676 796 1003 1070 1153 1174 1218
Size: 106 570 120 207 67 83 21 44
Fragments arranged by size:
570 207 120 106 83 67 44 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 311 888 1218
Size: 311 577 330
Fragments arranged by size:
577 330 311
EcoO109I rG'GnC_Cy
Cuts at: 0 304 468 999 1013 1218
Size: 304 164 531 14 205
Fragments arranged by size:
531 304 205 164 14
HaeII r_GCGC'y
Cuts at: 0 260 452 857 906 1218
Size: 260 192 405 49 312
Fragments arranged by size:
405 312 260 192 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 221 253 1082 1114 1218
Size: 221 32 829 32 104
Fragments arranged by size:
829 221 104 32 32
HincII GTy'rAC
Cuts at: 0 354 963 1118 1218
Size: 354 609 155 100
Fragments arranged by size:
609 354 155 100
KasI G'GCGC_C
Cuts at: 0 448 853 1218
Size: 448 405 365
Fragments arranged by size:
448 405 365
KpnI G_GTAC'C
Cuts at: 0 396 1218
Size: 396 822
MscI TGG'CCA
Cuts at: 0 589 1218
Size: 589 629
MslI CAynn'nnrTG
Cuts at: 0 484 1218
Size: 484 734
MspA1I CmG'CkG
Cuts at: 0 111 1140 1218
Size: 111 1029 78
Fragments arranged by size:
1029 111 78
NaeI GCC'GGC
Cuts at: 0 97 1218
Size: 97 1121
NarI GG'CG_CC
Cuts at: 0 449 854 1218
Size: 449 405 364
Fragments arranged by size:
449 405 364
NgoMIV G'CCGG_C
Cuts at: 0 95 1218
Size: 95 1123
PfoI T'CCnGG_A
Cuts at: 0 1076 1218
Size: 1076 142
PpuMI rG'GwC_Cy
Cuts at: 0 468 1218
Size: 468 750
PvuII CAG'CTG
Cuts at: 0 1140 1218
Size: 1140 78
SacI G_AGCT'C
Cuts at: 0 662 1218
Size: 662 556
SanDI GG'GwC_CC
Cuts at: 0 468 1218
Size: 468 750
SfoI GGC'GCC
Cuts at: 0 450 855 1218
Size: 450 405 363
Fragments arranged by size:
450 405 363
SmaI CCC'GGG
Cuts at: 0 173 1218
Size: 173 1045
SmlI C'TyrA_G
Cuts at: 0 661 956 1218
Size: 661 295 262
Fragments arranged by size:
661 295 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 123 369 1218
Size: 123 246 849
Fragments arranged by size:
849 246 123
TatI w'GTAC_w
Cuts at: 0 536 1218
Size: 536 682
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 403 747 1218
Size: 403 344 471
Fragments arranged by size:
471 403 344
XmaI C'CCGG_G
Cuts at: 0 171 1218
Size: 171 1047
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AhdI AleI AlwNI
AvaI BaeI BanI BanII BbeI BglI Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaHI BsaXI BseYI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BstYI Bsu36I BtgI
BtsI DraI DrdI EaeI EagI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII KasI KpnI MscI
MslI MspA1I NaeI NarI NgoMIV PfoI PpuMI PvuII
SacI SanDI SfoI SmaI SmlI TaqII TatI XcmI
XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AlfI AloI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BamHI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmrI
BmtI BplI BsaI BsaAI BsaBI BsaWI BseRI BsgI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI
DraIII EarI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI MfeI MluI MmeI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TstI Tth111I XbaI
XhoI XmnI ZraI