Restriction map for
pFN21AE2591
(Linear) (Six-base) MAPSORT of: KIEE2591.seq Check: 5711 from: 1 to: 1623
KIEE2591.orf
With 182 enzymes: SgfI *
October 13, 2015 18:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 401 418 946 1079 1623
Size: 394 7 17 528 133 544
Fragments arranged by size:
544 528 394 133 17 7
AflII C'TTAA_G
Cuts at: 0 106 1623
Size: 106 1517
AlwNI CAG_nnn'CTG
Cuts at: 0 235 427 1623
Size: 235 192 1196
Fragments arranged by size:
1196 235 192
ApoI r'AATT_y
Cuts at: 0 363 543 570 1236 1302 1492 1623
Size: 363 180 27 666 66 190 131
Fragments arranged by size:
666 363 190 180 131 66 27
BamHI G'GATC_C
Cuts at: 0 153 1623
Size: 153 1470
BanII G_rGCy'C
Cuts at: 0 994 1429 1623
Size: 994 435 194
Fragments arranged by size:
994 435 194
BbsI GAAGACnn'nnnn_
Cuts at: 0 194 1623
Size: 194 1429
BciVI GTATCCnnnnn_n'
Cuts at: 0 843 1623
Size: 843 780
BclI T'GATC_A
Cuts at: 0 1563 1623
Size: 1563 60
BlpI GC'TnA_GC
Cuts at: 0 4 1623
Size: 4 1619
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 672 959 1623
Size: 672 287 664
Fragments arranged by size:
672 664 287
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 509 1466 1519 1623
Size: 460 49 957 53 104
Fragments arranged by size:
957 460 104 53 49
BsaWI w'CCGG_w
Cuts at: 0 1328 1623
Size: 1328 295
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 640 1601 1623
Size: 640 961 22
Fragments arranged by size:
961 640 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1623
Size: 190 1433
BsiHKAI G_wGCw'C
Cuts at: 0 994 1623
Size: 994 629
Bsp1286I G_dGCh'C
Cuts at: 0 994 1429 1623
Size: 994 435 194
Fragments arranged by size:
994 435 194
BstAPI GCAn_nnn'nTGC
Cuts at: 0 427 595 1623
Size: 427 168 1028
Fragments arranged by size:
1028 427 168
BstYI r'GATC_y
Cuts at: 0 153 1623
Size: 153 1470
BtsI GCAGTG_nn'
Cuts at: 0 627 1494 1623
Size: 627 867 129
Fragments arranged by size:
867 627 129
ClaI AT'CG_AT
Cuts at: 0 1354 1623
Size: 1354 269
EarI CTCTTCn'nnn_
Cuts at: 0 95 437 548 1623
Size: 95 342 111 1075
Fragments arranged by size:
1075 342 111 95
EcoICRI GAG'CTC
Cuts at: 0 992 1623
Size: 992 631
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 401 418 672 946 959 1079 1623
Size: 394 7 17 254 274 13 120 544
Fragments arranged by size:
544 394 274 254 120 17 13 7
EcoRI G'AATT_C
Cuts at: 0 570 1302 1623
Size: 570 732 321
Fragments arranged by size:
732 570 321
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 790 1623
Size: 790 833
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 102 134 379 411 622 654 1366 1398
Size: 102 32 245 32 211 32 712 32
Cuts at: 1398 1453 1485 1623
Size: 55 32 138
Fragments arranged by size:
712 245 211 138 102 55 32 32
32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1224 1370 1496 1623
Size: 1224 146 126 127
Fragments arranged by size:
1224 146 127 126
PstI C_TGCA'G
Cuts at: 0 600 1623
Size: 600 1023
SacI G_AGCT'C
Cuts at: 0 994 1623
Size: 994 629
SapI GCTCTTCn'nnn_
Cuts at: 0 95 437 1623
Size: 95 342 1186
Fragments arranged by size:
1186 342 95
ScaI AGT'ACT
Cuts at: 0 972 1623
Size: 972 651
SfcI C'TryA_G
Cuts at: 0 236 596 1623
Size: 236 360 1027
Fragments arranged by size:
1027 360 236
SmlI C'TyrA_G
Cuts at: 0 106 475 524 1481 1498 1623
Size: 106 369 49 957 17 125
Fragments arranged by size:
957 369 125 106 49 17
StyI C'CwwG_G
Cuts at: 0 164 1623
Size: 164 1459
TatI w'GTAC_w
Cuts at: 0 937 970 1623
Size: 937 33 653
Fragments arranged by size:
937 653 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 195 227 1623
Size: 195 32 1396
Fragments arranged by size:
1396 195 32
Enzymes that do cut:
AcuI AflII AlwNI ApoI BamHI BanII BbsI BciVI
BclI BlpI BpmI BpuEI BsaWI BseRI BsgI BsiHKAI
Bsp1286I BstAPI BstYI BtsI ClaI EarI EcoICRI Eco57MI
EcoRI FalI Hin4I MmeI PstI SacI SapI ScaI
SfcI SmlI StyI TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI
BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoNI EcoO109I EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI