Restriction map for
pFN21AE2588
(Linear) (Six-base) MAPSORT of: KIEE2588.seq Check: 314 from: 1 to: 3429
KIEE2588.orf
With 182 enzymes: SgfI *
September 18, 2015 17:09 ..
AatII G_ACGT'C
Cuts at: 0 1232 3429
Size: 1232 2197
AccI GT'mk_AC
Cuts at: 0 2721 3429
Size: 2721 708
Acc65I G'GTAC_C
Cuts at: 0 916 3429
Size: 916 2513
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 1667 3429
Size: 215 1452 1762
Fragments arranged by size:
1762 1452 215
AfeI AGC'GCT
Cuts at: 0 188 3429
Size: 188 3241
AflIII A'CryG_T
Cuts at: 0 788 2535 2570 3429
Size: 788 1747 35 859
Fragments arranged by size:
1747 859 788 35
AgeI A'CCGG_T
Cuts at: 0 976 3429
Size: 976 2453
AhdI GACnn_n'nnGTC
Cuts at: 0 2262 3429
Size: 2262 1167
AleI CACnn'nnGTG
Cuts at: 0 598 2623 3429
Size: 598 2025 806
Fragments arranged by size:
2025 806 598
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 284 1550 3429
Size: 284 1266 1879
Fragments arranged by size:
1879 1266 284
AlwNI CAG_nnn'CTG
Cuts at: 0 170 483 526 640 1512 2921 3429
Size: 170 313 43 114 872 1409 508
Fragments arranged by size:
1409 872 508 313 170 114 43
ApaI G_GGCC'C
Cuts at: 0 85 2758 3429
Size: 85 2673 671
Fragments arranged by size:
2673 671 85
ApaLI G'TGCA_C
Cuts at: 0 1495 3429
Size: 1495 1934
ApoI r'AATT_y
Cuts at: 0 2208 3429
Size: 2208 1221
AseI AT'TA_AT
Cuts at: 0 1316 3429
Size: 1316 2113
AvaI C'yCGr_G
Cuts at: 0 1349 3429
Size: 1349 2080
BamHI G'GATC_C
Cuts at: 0 1305 3126 3429
Size: 1305 1821 303
Fragments arranged by size:
1821 1305 303
BanI G'GyrC_C
Cuts at: 0 742 916 2404 2674 3376 3429
Size: 742 174 1488 270 702 53
Fragments arranged by size:
1488 742 702 270 174 53
BanII G_rGCy'C
Cuts at: 0 85 110 211 461 701 1127 1573 2022
Size: 85 25 101 250 240 426 446 449
Cuts at: 2022 2329 2750 2758 2882 2936 3010 3308 3429
Size: 307 421 8 124 54 74 298 121
Fragments arranged by size:
449 446 426 421 307 298 250 240
124 121 101 85 74 54 25 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 203 3429
Size: 203 3226
BbvCI CC'TCA_GC
Cuts at: 0 126 544 1292 1448 1664 2136 3429
Size: 126 418 748 156 216 472 1293
Fragments arranged by size:
1293 748 472 418 216 156 126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1889 1923 3429
Size: 1889 34 1506
Fragments arranged by size:
1889 1506 34
BglI GCCn_nnn'nGGC
Cuts at: 0 2852 3014 3429
Size: 2852 162 415
Fragments arranged by size:
2852 415 162
BlpI GC'TnA_GC
Cuts at: 0 104 2876 3429
Size: 104 2772 553
Fragments arranged by size:
2772 553 104
Bme1580I G_kGCm'C
Cuts at: 0 85 1349 1499 2578 2758 3429
Size: 85 1264 150 1079 180 671
Fragments arranged by size:
1264 1079 671 180 150 85
BmgBI CAC'GTC
Cuts at: 0 752 804 3429
Size: 752 52 2625
Fragments arranged by size:
2625 752 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 1995 3429
Size: 1995 1434
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 729 1444 2325 3429
Size: 729 715 881 1104
Fragments arranged by size:
1104 881 729 715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 1439 1442 1637 2335 2734 2974 3429
Size: 715 724 3 195 698 399 240 455
Fragments arranged by size:
724 715 698 455 399 240 195 3
Bpu10I CC'TnA_GC
Cuts at: 0 126 142 278 544 1292 1448 1664 2136
Size: 126 16 136 266 748 156 216 472
Cuts at: 2136 3429
Size: 1293
Fragments arranged by size:
1293 748 472 266 216 156 136 126
16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 2212 2497 3429
Size: 865 1347 285 932
Fragments arranged by size:
1347 932 865 285
BsaI GGTCTCn'nnnn_
Cuts at: 0 717 2099 3429
Size: 717 1382 1330
Fragments arranged by size:
1382 1330 717
BsaAI yAC'GTr
Cuts at: 0 789 2573 3429
Size: 789 1784 856
Fragments arranged by size:
1784 856 789
BsaHI Gr'CG_yC
Cuts at: 0 1229 3429
Size: 1229 2200
BsaWI w'CCGG_w
Cuts at: 0 976 1361 2884 3386 3429
Size: 976 385 1523 502 43
Fragments arranged by size:
1523 976 502 385 43
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1981 2011 3429
Size: 1981 30 1418
Fragments arranged by size:
1981 1418 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 565 979 2124 2602 2785 2944 3429
Size: 565 414 1145 478 183 159 485
Fragments arranged by size:
1145 565 485 478 414 183 159
BseYI C'CCAG_C
Cuts at: 0 84 109 808 955 1287 1659 1712 1741
Size: 84 25 699 147 332 372 53 29
Cuts at: 1741 1762 1882 1941 2890 3012 3429
Size: 21 120 59 949 122 417
Fragments arranged by size:
949 699 417 372 332 147 122 120
84 59 53 29 25 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 244 862 1011 2106 3429
Size: 74 170 618 149 1095 1323
Fragments arranged by size:
1323 1095 618 170 149 74
BsiEI CG_ry'CG
Cuts at: 0 1959 3285 3429
Size: 1959 1326 144
Fragments arranged by size:
1959 1326 144
BsiHKAI G_wGCw'C
Cuts at: 0 211 284 557 1127 1499 2750 2882 2909
Size: 211 73 273 570 372 1251 132 27
Cuts at: 2909 3182 3429
Size: 273 247
Fragments arranged by size:
1251 570 372 273 273 247 211 132
73 27
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2696 3429
Size: 2696 733
Bsp1286I G_dGCh'C
Cuts at: 0 85 110 211 284 461 557 701 1127
Size: 85 25 101 73 177 96 144 426
Cuts at: 1127 1349 1499 1573 2022 2329 2578 2750 2758
Size: 222 150 74 449 307 249 172 8
Cuts at: 2758 2882 2909 2936 3010 3182 3308 3429
Size: 124 27 27 74 172 126 121
Fragments arranged by size:
449 426 307 249 222 177 172 172
150 144 126 124 121 101 96 85
74 74 73 27 27 25 8
BspEI T'CCGG_A
Cuts at: 0 1361 3429
Size: 1361 2068
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 104 1589 1984 3429
Size: 104 1485 395 1445
Fragments arranged by size:
1485 1445 395 104
BsrBI CCG'CTC
Cuts at: 0 998 1722 2074 3429
Size: 998 724 352 1355
Fragments arranged by size:
1355 998 724 352
BsrDI GCAATG_nn'
Cuts at: 0 1242 3429
Size: 1242 2187
BsrFI r'CCGG_y
Cuts at: 0 976 2401 3429
Size: 976 1425 1028
Fragments arranged by size:
1425 1028 976
BsrGI T'GTAC_A
Cuts at: 0 247 3429
Size: 247 3182
BstEII G'GTnAC_C
Cuts at: 0 2610 3429
Size: 2610 819
BstXI CCAn_nnnn'nTGG
Cuts at: 0 739 1224 2898 2946 3429
Size: 739 485 1674 48 483
Fragments arranged by size:
1674 739 485 483 48
BstYI r'GATC_y
Cuts at: 0 428 1305 1358 1581 2663 3126 3429
Size: 428 877 53 223 1082 463 303
Fragments arranged by size:
1082 877 463 428 303 223 53
BstZ17I GTA'TAC
Cuts at: 0 2722 3429
Size: 2722 707
Bsu36I CC'TnA_GG
Cuts at: 0 345 2564 3429
Size: 345 2219 865
Fragments arranged by size:
2219 865 345
BtgI C'CryG_G
Cuts at: 0 301 701 1071 1270 1772 1936 2156 3429
Size: 301 400 370 199 502 164 220 1273
Fragments arranged by size:
1273 502 400 370 301 220 199 164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 752 3429
Size: 752 2677
BtsI GCAGTG_nn'
Cuts at: 0 98 261 315 1532 1683 1824 2189 2327
Size: 98 163 54 1217 151 141 365 138
Cuts at: 2327 3024 3429
Size: 697 405
Fragments arranged by size:
1217 697 405 365 163 151 141 138
98 54
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1224 1259 3429
Size: 1224 35 2170
Fragments arranged by size:
2170 1224 35
DraIII CAC_nnn'GTG
Cuts at: 0 1344 3429
Size: 1344 2085
EaeI y'GGCC_r
Cuts at: 0 489 570 858 1214 1335 1775 2153 2421
Size: 489 81 288 356 121 440 378 268
Cuts at: 2421 3429
Size: 1008
Fragments arranged by size:
1008 489 440 378 356 288 268 121
81
EarI CTCTTCn'nnn_
Cuts at: 0 23 646 897 1958 2316 3274 3429
Size: 23 623 251 1061 358 958 155
Fragments arranged by size:
1061 958 623 358 251 155 23
EcoICRI GAG'CTC
Cuts at: 0 209 1125 2748 2880 3429
Size: 209 916 1623 132 549
Fragments arranged by size:
1623 916 549 209 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 715 1439 1442 1637 1667 2335 2734
Size: 215 500 724 3 195 30 668 399
Cuts at: 2734 2974 3429
Size: 240 455
Fragments arranged by size:
724 668 500 455 399 240 215 195
30 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 547 1457 3429
Size: 547 910 1972
Fragments arranged by size:
1972 910 547
EcoO109I rG'GnC_Cy
Cuts at: 0 81 480 540 1284 2172 2754 2755 2825
Size: 81 399 60 744 888 582 1 70
Cuts at: 2825 3023 3429
Size: 198 406
Fragments arranged by size:
888 744 582 406 399 198 81 70
60 1
EcoRI G'AATT_C
Cuts at: 0 2208 3429
Size: 2208 1221
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 417 2253 3429
Size: 417 1836 1176
Fragments arranged by size:
1836 1176 417
HaeII r_GCGC'y
Cuts at: 0 190 672 1513 3429
Size: 190 482 841 1916
Fragments arranged by size:
1916 841 482 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1343 1375 1908 1940 1981 2013 2248 2280
Size: 1343 32 533 32 41 32 235 32
Cuts at: 2280 3111 3143 3392 3399 3424 3429
Size: 831 32 249 7 25 5
Fragments arranged by size:
1343 831 533 249 235 41 32 32
32 32 32 25 7 5
HincII GTy'rAC
Cuts at: 0 2415 3429
Size: 2415 1014
HindIII A'AGCT_T
Cuts at: 0 449 3429
Size: 449 2980
KpnI G_GTAC'C
Cuts at: 0 920 3429
Size: 920 2509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 879 2074 2488 2531 3097 3351 3429
Size: 879 1195 414 43 566 254 78
Fragments arranged by size:
1195 879 566 414 254 78 43
MscI TGG'CCA
Cuts at: 0 491 860 1216 1337 1777 2155 2423 3429
Size: 491 369 356 121 440 378 268 1006
Fragments arranged by size:
1006 491 440 378 369 356 268 121
MslI CAynn'nnrTG
Cuts at: 0 598 2623 3429
Size: 598 2025 806
Fragments arranged by size:
2025 806 598
MspA1I CmG'CkG
Cuts at: 0 88 303 1220 1291 1663 1745 1800 1995
Size: 88 215 917 71 372 82 55 195
Cuts at: 1995 2658 2872 3012 3429
Size: 663 214 140 417
Fragments arranged by size:
917 663 417 372 215 214 195 140
88 82 71 55
NspI r_CATG'y
Cuts at: 0 1692 3429
Size: 1692 1737
PasI CC'CwG_GG
Cuts at: 0 2167 3429
Size: 2167 1262
PflMI CCAn_nnn'nTGG
Cuts at: 0 1875 2921 3429
Size: 1875 1046 508
Fragments arranged by size:
1875 1046 508
PfoI T'CCnGG_A
Cuts at: 0 692 2218 2351 2372 2438 2666 2683 3429
Size: 692 1526 133 21 66 228 17 746
Fragments arranged by size:
1526 746 692 228 133 66 21 17
PmlI CAC'GTG
Cuts at: 0 2573 3429
Size: 2573 856
PpuMI rG'GwC_Cy
Cuts at: 0 480 1284 2825 3429
Size: 480 804 1541 604
Fragments arranged by size:
1541 804 604 480
PshAI GACnn'nnGTC
Cuts at: 0 721 3429
Size: 721 2708
PspOMI G'GGCC_C
Cuts at: 0 81 2754 3429
Size: 81 2673 675
Fragments arranged by size:
2673 675 81
PstI C_TGCA'G
Cuts at: 0 1973 2657 3429
Size: 1973 684 772
Fragments arranged by size:
1973 772 684
PvuII CAG'CTG
Cuts at: 0 88 1220 1291 1663 1800 1995 3429
Size: 88 1132 71 372 137 195 1434
Fragments arranged by size:
1434 1132 372 195 137 88 71
SacI G_AGCT'C
Cuts at: 0 211 1127 2750 2882 3429
Size: 211 916 1623 132 547
Fragments arranged by size:
1623 916 547 211 132
SacII CC_GC'GG
Cuts at: 0 304 3429
Size: 304 3125
SapI GCTCTTCn'nnn_
Cuts at: 0 897 1958 3429
Size: 897 1061 1471
Fragments arranged by size:
1471 1061 897
ScaI AGT'ACT
Cuts at: 0 2983 3429
Size: 2983 446
SfcI C'TryA_G
Cuts at: 0 1164 1969 2653 3429
Size: 1164 805 684 776
Fragments arranged by size:
1164 805 776 684
SgrAI Cr'CCGG_yG
Cuts at: 0 976 3429
Size: 976 2453
SmlI C'TyrA_G
Cuts at: 0 880 2227 2476 3429
Size: 880 1347 249 953
Fragments arranged by size:
1347 953 880 249
SspI AAT'ATT
Cuts at: 0 243 3429
Size: 243 3186
StuI AGG'CCT
Cuts at: 0 1904 3371 3429
Size: 1904 1467 58
Fragments arranged by size:
1904 1467 58
StyI C'CwwG_G
Cuts at: 0 1262 1309 1517 1538 1790 2150 2315 2418
Size: 1262 47 208 21 252 360 165 103
Cuts at: 2418 2424 2593 2939 3219 3429
Size: 6 169 346 280 210
Fragments arranged by size:
1262 360 346 280 252 210 208 169
165 103 47 21 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1008 1235 1973 2875 3364 3429
Size: 1008 227 738 902 489 65
Fragments arranged by size:
1008 902 738 489 227 65
TatI w'GTAC_w
Cuts at: 0 247 2981 2993 3429
Size: 247 2734 12 436
Fragments arranged by size:
2734 436 247 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2527 2559 3429
Size: 2527 32 870
Fragments arranged by size:
2527 870 32
Tth111I GACn'n_nGTC
Cuts at: 0 1471 3429
Size: 1471 1958
XbaI T'CTAG_A
Cuts at: 0 1926 3429
Size: 1926 1503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 405 484 641 1797 3009 3429
Size: 405 79 157 1156 1212 420
Fragments arranged by size:
1212 1156 420 405 157 79
XmnI GAAnn'nnTTC
Cuts at: 0 2045 3429
Size: 2045 1384
ZraI GAC'GTC
Cuts at: 0 1230 3429
Size: 1230 2199
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI
BamHI BanI BanII BbsI BbvCI BcgI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII KpnI
MmeI MscI MslI MspA1I NspI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PstI PvuII SacI SacII
SapI ScaI SfcI SgrAI SmlI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AclI AflII AloI AscI AsiSI AvrII BaeI
BbeI BciVI BclI BfrBI BglII BmtI BsaBI BsiWI
BsmI BspHI BssHII BssSI BstAPI BstBI ClaI DraI
DrdI EagI EciI EcoRV FseI FspI FspAI HpaI
KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PpiI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SphI
SrfI SwaI XhoI XmaI