Restriction map for
pFN21AE2581
(Linear) (Six-base) MAPSORT of: KIEE2581.seq Check: 4114 from: 1 to: 3057
KIEE2581.orf
With 182 enzymes: SgfI *
July 22, 2015 09:50 ..
AatII G_ACGT'C
Cuts at: 0 2829 3057
Size: 2829 228
AccI GT'mk_AC
Cuts at: 0 2435 3057
Size: 2435 622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 572 996 3057
Size: 177 395 424 2061
Fragments arranged by size:
2061 424 395 177
AflIII A'CryG_T
Cuts at: 0 2430 3057
Size: 2430 627
AleI CACnn'nnGTG
Cuts at: 0 323 655 1348 3057
Size: 323 332 693 1709
Fragments arranged by size:
1709 693 332 323
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2647 2679 3057
Size: 2647 32 378
Fragments arranged by size:
2647 378 32
AlwNI CAG_nnn'CTG
Cuts at: 0 972 1533 1548 1836 3057
Size: 972 561 15 288 1221
Fragments arranged by size:
1221 972 561 288 15
ApoI r'AATT_y
Cuts at: 0 1279 2778 2818 3057
Size: 1279 1499 40 239
Fragments arranged by size:
1499 1279 239 40
AseI AT'TA_AT
Cuts at: 0 1805 3057
Size: 1805 1252
AvaI C'yCGr_G
Cuts at: 0 132 3057
Size: 132 2925
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2628 2661 3057
Size: 2628 33 396
Fragments arranged by size:
2628 396 33
BamHI G'GATC_C
Cuts at: 0 1956 3057
Size: 1956 1101
BanI G'GyrC_C
Cuts at: 0 1320 1733 2927 3057
Size: 1320 413 1194 130
Fragments arranged by size:
1320 1194 413 130
BanII G_rGCy'C
Cuts at: 0 146 1949 2048 3057
Size: 146 1803 99 1009
Fragments arranged by size:
1803 1009 146 99
BbsI GAAGACnn'nnnn_
Cuts at: 0 2840 2933 3057
Size: 2840 93 124
Fragments arranged by size:
2840 124 93
BbvCI CC'TCA_GC
Cuts at: 0 350 1526 3057
Size: 350 1176 1531
Fragments arranged by size:
1531 1176 350
BclI T'GATC_A
Cuts at: 0 1910 2963 3057
Size: 1910 1053 94
Fragments arranged by size:
1910 1053 94
BglI GCCn_nnn'nGGC
Cuts at: 0 488 714 827 1792 2264 3057
Size: 488 226 113 965 472 793
Fragments arranged by size:
965 793 488 472 226 113
Bme1580I G_kGCm'C
Cuts at: 0 560 662 2932 3057
Size: 560 102 2270 125
Fragments arranged by size:
2270 560 125 102
BmgBI CAC'GTC
Cuts at: 0 2433 3057
Size: 2433 624
BmrI ACTGGGnnnn_n'
Cuts at: 0 1342 2167 2931 3057
Size: 1342 825 764 126
Fragments arranged by size:
1342 825 764 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 326 2301 2608 2758 3057
Size: 200 126 1975 307 150 299
Fragments arranged by size:
1975 307 299 200 150 126
Bpu10I CC'TnA_GC
Cuts at: 0 350 1526 1943 2042 3057
Size: 350 1176 417 99 1015
Fragments arranged by size:
1176 1015 417 350 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2133 2392 3057
Size: 2133 259 665
Fragments arranged by size:
2133 665 259
BsaAI yAC'GTr
Cuts at: 0 657 3057
Size: 657 2400
BsaHI Gr'CG_yC
Cuts at: 0 2335 2594 2826 3057
Size: 2335 259 232 231
Fragments arranged by size:
2335 259 232 231
BsaWI w'CCGG_w
Cuts at: 0 594 702 3057
Size: 594 108 2355
Fragments arranged by size:
2355 594 108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 402 432 1300 1330 1501 1531 3057
Size: 402 30 868 30 171 30 1526
Fragments arranged by size:
1526 868 402 171 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1322 1381 1507 1615 2581 2878 3057
Size: 1322 59 126 108 966 297 179
Fragments arranged by size:
1322 966 297 179 126 108 59
BseYI C'CCAG_C
Cuts at: 0 678 1259 1470 1541 2267 3057
Size: 678 581 211 71 726 790
Fragments arranged by size:
790 726 678 581 211 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1129 1358 3057
Size: 1129 229 1699
Fragments arranged by size:
1699 1129 229
BsiEI CG_ry'CG
Cuts at: 0 810 822 3057
Size: 810 12 2235
Fragments arranged by size:
2235 810 12
BsiHKAI G_wGCw'C
Cuts at: 0 2040 2291 3057
Size: 2040 251 766
Fragments arranged by size:
2040 766 251
BsiWI C'GTAC_G
Cuts at: 0 892 3057
Size: 892 2165
Bsp1286I G_dGCh'C
Cuts at: 0 146 560 662 1949 2040 2048 2291 2932
Size: 146 414 102 1287 91 8 243 641
Cuts at: 2932 3057
Size: 125
Fragments arranged by size:
1287 641 414 243 146 125 102 91
8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 621 3057
Size: 621 2436
BsrBI CCG'CTC
Cuts at: 0 2063 2469 3057
Size: 2063 406 588
Fragments arranged by size:
2063 588 406
BsrDI GCAATG_nn'
Cuts at: 0 166 2371 3057
Size: 166 2205 686
Fragments arranged by size:
2205 686 166
BsrFI r'CCGG_y
Cuts at: 0 1730 2989 3057
Size: 1730 1259 68
Fragments arranged by size:
1730 1259 68
BssSI C'ACGA_G
Cuts at: 0 1334 3057
Size: 1334 1723
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1214 2731 2781 3057
Size: 1214 1517 50 276
Fragments arranged by size:
1517 1214 276 50
BstYI r'GATC_y
Cuts at: 0 1694 1956 2847 3057
Size: 1694 262 891 210
Fragments arranged by size:
1694 891 262 210
BtgI C'CryG_G
Cuts at: 0 662 780 786 2855 3057
Size: 662 118 6 2069 202
Fragments arranged by size:
2069 662 202 118 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2040 3057
Size: 2040 1017
BtsI GCAGTG_nn'
Cuts at: 0 1637 3057
Size: 1637 1420
DraIII CAC_nnn'GTG
Cuts at: 0 1107 2889 3057
Size: 1107 1782 168
Fragments arranged by size:
1782 1107 168
DrdI GACnn_nn'nnGTC
Cuts at: 0 1684 3057
Size: 1684 1373
EaeI y'GGCC_r
Cuts at: 0 783 807 819 1462 1793 2018 2487 2721
Size: 783 24 12 643 331 225 469 234
Cuts at: 2721 2991 3057
Size: 270 66
Fragments arranged by size:
783 643 469 331 270 234 225 66
24 12
EagI C'GGCC_G
Cuts at: 0 807 819 3057
Size: 807 12 2238
Fragments arranged by size:
2238 807 12
EarI CTCTTCn'nnn_
Cuts at: 0 739 1226 1447 1822 2518 3057
Size: 739 487 221 375 696 539
Fragments arranged by size:
739 696 539 487 375 221
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1638 3057
Size: 1638 1419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 200 326 572 996 2301 2608 2758
Size: 177 23 126 246 424 1305 307 150
Cuts at: 2758 3057
Size: 299
Fragments arranged by size:
1305 424 307 299 246 177 150 126
23
EcoO109I rG'GnC_Cy
Cuts at: 0 886 1113 1398 1833 2111 2294 3057
Size: 886 227 285 435 278 183 763
Fragments arranged by size:
886 763 435 285 278 227 183
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1650 3057
Size: 1650 1407
HaeII r_GCGC'y
Cuts at: 0 996 1787 2457 3057
Size: 996 791 670 600
Fragments arranged by size:
996 791 670 600
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 272 304 718 750 1300 1332 1642 1674
Size: 272 32 414 32 550 32 310 32
Cuts at: 1674 3057
Size: 1383
Fragments arranged by size:
1383 550 414 310 272 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1689 3057
Size: 1689 1368
MfeI C'AATT_G
Cuts at: 0 291 3057
Size: 291 2766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2847 3057
Size: 2847 210
MscI TGG'CCA
Cuts at: 0 1795 2020 2489 2723 3057
Size: 1795 225 469 234 334
Fragments arranged by size:
1795 469 334 234 225
MslI CAynn'nnrTG
Cuts at: 0 323 655 1348 3057
Size: 323 332 693 1709
Fragments arranged by size:
1709 693 332 323
MspA1I CmG'CkG
Cuts at: 0 127 238 1533 1548 2580 3057
Size: 127 111 1295 15 1032 477
Fragments arranged by size:
1295 1032 477 127 111 15
NaeI GCC'GGC
Cuts at: 0 1732 3057
Size: 1732 1325
NcoI C'CATG_G
Cuts at: 0 2855 3057
Size: 2855 202
NgoMIV G'CCGG_C
Cuts at: 0 1730 3057
Size: 1730 1327
NspI r_CATG'y
Cuts at: 0 1728 3057
Size: 1728 1329
PasI CC'CwG_GG
Cuts at: 0 97 1738 3057
Size: 97 1641 1319
Fragments arranged by size:
1641 1319 97
PfoI T'CCnGG_A
Cuts at: 0 3002 3057
Size: 3002 55
PpuMI rG'GwC_Cy
Cuts at: 0 1833 2111 3057
Size: 1833 278 946
Fragments arranged by size:
1833 946 278
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2554 2586 3057
Size: 2554 32 471
Fragments arranged by size:
2554 471 32
PvuII CAG'CTG
Cuts at: 0 127 238 1533 1548 2580 3057
Size: 127 111 1295 15 1032 477
Fragments arranged by size:
1295 1032 477 127 111 15
SfcI C'TryA_G
Cuts at: 0 2339 2607 2640 3057
Size: 2339 268 33 417
Fragments arranged by size:
2339 417 268 33
SmlI C'TyrA_G
Cuts at: 0 2148 2407 3057
Size: 2148 259 650
Fragments arranged by size:
2148 650 259
SspI AAT'ATT
Cuts at: 0 2764 3057
Size: 2764 293
StuI AGG'CCT
Cuts at: 0 30 1458 2952 3057
Size: 30 1428 1494 105
Fragments arranged by size:
1494 1428 105 30
StyI C'CwwG_G
Cuts at: 0 119 221 1394 1556 1796 2249 2855 3057
Size: 119 102 1173 162 240 453 606 202
Fragments arranged by size:
1173 606 453 240 202 162 119 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 59 387 1723 1751 2259 2848 3000 3057
Size: 59 328 1336 28 508 589 152 57
Fragments arranged by size:
1336 589 508 328 152 59 57 28
TatI w'GTAC_w
Cuts at: 0 902 3057
Size: 902 2155
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1501 1533 1976 2008 3057
Size: 1501 32 443 32 1049
Fragments arranged by size:
1501 1049 443 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 934 1314 3057
Size: 934 380 1743
Fragments arranged by size:
1743 934 380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 567 3057
Size: 567 2490
XmnI GAAnn'nnTTC
Cuts at: 0 1283 1633 3057
Size: 1283 350 1424
Fragments arranged by size:
1424 1283 350
ZraI GAC'GTC
Cuts at: 0 2827 3057
Size: 2827 230
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AloI AlwNI ApoI
AseI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BclI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI
BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I
HincII MfeI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PasI PfoI PpuMI PsrI PvuII SfcI
SmlI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI
ApaI ApaLI AscI AsiSI AvrII BbeI BcgI BciVI
BfrBI BglII BlpI BmtI BplI BsaI BsaBI BsmI
BsmBI BspEI BspHI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI
MluI NarI NdeI NheI NotI NruI NsiI PacI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI XbaI XhoI
XmaI