Restriction map for
pFN21AE2574
(Linear) (Six-base) MAPSORT of: KIEE2574.seq Check: 1569 from: 1 to: 1323
KIEE2574.orf
With 182 enzymes: SgfI *
April 21, 2015 11:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 579 616 1188 1323
Size: 579 37 572 135
Fragments arranged by size:
579 572 135 37
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 1011 1323
Size: 430 581 312
Fragments arranged by size:
581 430 312
AfeI AGC'GCT
Cuts at: 0 11 1323
Size: 11 1312
AflIII A'CryG_T
Cuts at: 0 769 1323
Size: 769 554
ApaI G_GGCC'C
Cuts at: 0 45 1323
Size: 45 1278
ApoI r'AATT_y
Cuts at: 0 299 383 434 746 1089 1159 1323
Size: 299 84 51 312 343 70 164
Fragments arranged by size:
343 312 299 164 84 70 51
AvaI C'yCGr_G
Cuts at: 0 483 1323
Size: 483 840
AvrII C'CTAG_G
Cuts at: 0 930 1323
Size: 930 393
BanI G'GyrC_C
Cuts at: 0 3 28 1323
Size: 3 25 1295
Fragments arranged by size:
1295 25 3
BanII G_rGCy'C
Cuts at: 0 45 1323
Size: 45 1278
Bme1580I G_kGCm'C
Cuts at: 0 45 1323
Size: 45 1278
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 1323
Size: 454 869
BsaWI w'CCGG_w
Cuts at: 0 903 1323
Size: 903 420
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 346 1323
Size: 346 977
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 794 798 1323
Size: 368 426 4 525
Fragments arranged by size:
525 426 368 4
BsiHKAI G_wGCw'C
Cuts at: 0 198 1323
Size: 198 1125
BsmI GAATG_Cn'
Cuts at: 0 710 1323
Size: 710 613
Bsp1286I G_dGCh'C
Cuts at: 0 45 198 1323
Size: 45 153 1125
Fragments arranged by size:
1125 153 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 579 616 863 1188 1323
Size: 579 37 247 325 135
Fragments arranged by size:
579 325 247 135 37
BsrFI r'CCGG_y
Cuts at: 0 58 917 1323
Size: 58 859 406
Fragments arranged by size:
859 406 58
BsrGI T'GTAC_A
Cuts at: 0 339 1323
Size: 339 984
BstAPI GCAn_nnn'nTGC
Cuts at: 0 991 1323
Size: 991 332
Bsu36I CC'TnA_GG
Cuts at: 0 490 1323
Size: 490 833
BtgI C'CryG_G
Cuts at: 0 45 1323
Size: 45 1278
BtsI GCAGTG_nn'
Cuts at: 0 403 1323
Size: 403 920
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 381 416 1323
Size: 381 35 907
Fragments arranged by size:
907 381 35
DraI TTT'AAA
Cuts at: 0 999 1320 1323
Size: 999 321 3
Fragments arranged by size:
999 321 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 182 1323
Size: 182 1141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 454 1011 1323
Size: 430 24 557 312
Fragments arranged by size:
557 430 312 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 929 1323
Size: 929 394
EcoO109I rG'GnC_Cy
Cuts at: 0 450 976 1323
Size: 450 526 347
Fragments arranged by size:
526 450 347
HaeII r_GCGC'y
Cuts at: 0 13 131 1323
Size: 13 118 1192
Fragments arranged by size:
1192 118 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 185 217 1323
Size: 185 32 1106
Fragments arranged by size:
1106 185 32
HindIII A'AGCT_T
Cuts at: 0 406 1323
Size: 406 917
MfeI C'AATT_G
Cuts at: 0 716 1323
Size: 716 607
MslI CAynn'nnrTG
Cuts at: 0 278 1243 1323
Size: 278 965 80
Fragments arranged by size:
965 278 80
MspA1I CmG'CkG
Cuts at: 0 47 108 1323
Size: 47 61 1215
Fragments arranged by size:
1215 61 47
NspI r_CATG'y
Cuts at: 0 773 1127 1323
Size: 773 354 196
Fragments arranged by size:
773 354 196
PasI CC'CwG_GG
Cuts at: 0 70 1323
Size: 70 1253
PciI A'CATG_T
Cuts at: 0 769 1323
Size: 769 554
PflMI CCAn_nnn'nTGG
Cuts at: 0 568 1323
Size: 568 755
PsiI TTA'TAA
Cuts at: 0 308 724 1323
Size: 308 416 599
Fragments arranged by size:
599 416 308
PspOMI G'GGCC_C
Cuts at: 0 41 1323
Size: 41 1282
SacII CC_GC'GG
Cuts at: 0 48 1323
Size: 48 1275
ScaI AGT'ACT
Cuts at: 0 367 1323
Size: 367 956
SgrAI Cr'CCGG_yG
Cuts at: 0 58 1323
Size: 58 1265
SmaI CCC'GGG
Cuts at: 0 485 1323
Size: 485 838
SspI AAT'ATT
Cuts at: 0 897 1323
Size: 897 426
StuI AGG'CCT
Cuts at: 0 467 1323
Size: 467 856
StyI C'CwwG_G
Cuts at: 0 930 949 1323
Size: 930 19 374
Fragments arranged by size:
930 374 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1230 1323
Size: 1230 93
TatI w'GTAC_w
Cuts at: 0 339 365 1323
Size: 339 26 958
Fragments arranged by size:
958 339 26
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 807 839 1323
Size: 807 32 484
Fragments arranged by size:
807 484 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 625 1041 1323
Size: 625 416 282
Fragments arranged by size:
625 416 282
XmaI C'CCGG_G
Cuts at: 0 483 1323
Size: 483 840
XmnI GAAnn'nnTTC
Cuts at: 0 434 1323
Size: 434 889
Enzymes that do cut:
AarI AcuI AfeI AflIII ApaI ApoI AvaI AvrII
BanI BanII Bme1580I BpmI BsaWI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrFI BsrGI BstAPI Bsu36I BtgI
BtsI CspCI DraI EciI Eco57MI EcoNI EcoO109I HaeII
Hin4I HindIII MfeI MslI MspA1I NspI PasI PciI
PflMI PsiI PspOMI SacII ScaI SgrAI SmaI SspI
StuI StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseYI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BssHII BssSI
BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI DraIII
DrdI EaeI EagI EarI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HincII HpaI KasI KpnI MluI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PfoI PmeI PmlI PpiI
PpuMI PshAI PspXI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI SexAI SfcI SfiI
SfoI SgfI SmlI SnaBI SpeI SphI SrfI SwaI
Tth111I XbaI XhoI ZraI