Restriction map for
pFN21AE2573
(Linear) (Six-base) MAPSORT of: KIEE2573.seq Check: 8966 from: 1 to: 2325
KIEE2573.orf
With 182 enzymes: SgfI *
May 7, 2015 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 536 557 2325
Size: 536 21 1768
Fragments arranged by size:
1768 536 21
AfeI AGC'GCT
Cuts at: 0 2188 2325
Size: 2188 137
AflIII A'CryG_T
Cuts at: 0 995 2325
Size: 995 1330
AleI CACnn'nnGTG
Cuts at: 0 97 862 2325
Size: 97 765 1463
Fragments arranged by size:
1463 765 97
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 781 2325
Size: 781 1544
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1813 1845 2325
Size: 1813 32 480
Fragments arranged by size:
1813 480 32
AlwNI CAG_nnn'CTG
Cuts at: 0 354 1341 2325
Size: 354 987 984
Fragments arranged by size:
987 984 354
ApaI G_GGCC'C
Cuts at: 0 44 477 1976 2199 2325
Size: 44 433 1499 223 126
Fragments arranged by size:
1499 433 223 126 44
ApaLI G'TGCA_C
Cuts at: 0 892 991 2325
Size: 892 99 1334
Fragments arranged by size:
1334 892 99
AscI GG'CGCG_CC
Cuts at: 0 175 2325
Size: 175 2150
AvaI C'yCGr_G
Cuts at: 0 220 1022 1373 1739 2325
Size: 220 802 351 366 586
Fragments arranged by size:
802 586 366 351 220
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1027 1060 2325
Size: 1027 33 1265
Fragments arranged by size:
1265 1027 33
BanI G'GyrC_C
Cuts at: 0 235 425 591 1735 1743 2066 2325
Size: 235 190 166 1144 8 323 259
Fragments arranged by size:
1144 323 259 235 190 166 8
BanII G_rGCy'C
Cuts at: 0 44 477 710 919 1976 2199 2325
Size: 44 433 233 209 1057 223 126
Fragments arranged by size:
1057 433 233 223 209 126 44
BbeI G_GCGC'C
Cuts at: 0 239 2070 2325
Size: 239 1831 255
Fragments arranged by size:
1831 255 239
BbsI GAAGACnn'nnnn_
Cuts at: 0 1677 2325
Size: 1677 648
BbvCI CC'TCA_GC
Cuts at: 0 646 2325
Size: 646 1679
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 789 823 2325
Size: 789 34 1502
Fragments arranged by size:
1502 789 34
BfrBI ATG'CAT
Cuts at: 0 1890 1926 2325
Size: 1890 36 399
Fragments arranged by size:
1890 399 36
BglI GCCn_nnn'nGGC
Cuts at: 0 39 304 638 734 1708 2026 2325
Size: 39 265 334 96 974 318 299
Fragments arranged by size:
974 334 318 299 265 96 39
BglII A'GATC_T
Cuts at: 0 1484 2325
Size: 1484 841
BlpI GC'TnA_GC
Cuts at: 0 85 913 1471 1550 2325
Size: 85 828 558 79 775
Fragments arranged by size:
828 775 558 85 79
Bme1580I G_kGCm'C
Cuts at: 0 44 428 477 896 963 995 1499 1740
Size: 44 384 49 419 67 32 504 241
Cuts at: 1740 1976 2199 2325
Size: 236 223 126
Fragments arranged by size:
504 419 384 241 236 223 126 67
49 44 32
BmgBI CAC'GTC
Cuts at: 0 1519 2204 2325
Size: 1519 685 121
Fragments arranged by size:
1519 685 121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 454 574 928 1312 1426 1621 1738
Size: 155 299 120 354 384 114 195 117
Cuts at: 1738 2125 2127 2230 2325
Size: 387 2 103 95
Fragments arranged by size:
387 384 354 299 195 155 120 117
114 103 95 2
Bpu10I CC'TnA_GC
Cuts at: 0 76 646 2325
Size: 76 570 1679
Fragments arranged by size:
1679 570 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2278 2325
Size: 2278 47
BsaAI yAC'GTr
Cuts at: 0 1043 1707 2052 2325
Size: 1043 664 345 273
Fragments arranged by size:
1043 664 345 273
BsaHI Gr'CG_yC
Cuts at: 0 236 2067 2325
Size: 236 1831 258
Fragments arranged by size:
1831 258 236
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 94 124 1051 1081 1192 1222 2325
Size: 94 30 927 30 111 30 1103
Fragments arranged by size:
1103 927 111 94 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 523 1306 2110 2325
Size: 118 405 783 804 215
Fragments arranged by size:
804 783 405 215 118
BseYI C'CCAG_C
Cuts at: 0 336 396 420 501 834 1178 1661 1944
Size: 336 60 24 81 333 344 483 283
Cuts at: 1944 1983 2279 2325
Size: 39 296 46
Fragments arranged by size:
483 344 336 333 296 283 81 60
46 39 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 263 514 855 952 991 1474 1780
Size: 214 49 251 341 97 39 483 306
Cuts at: 1780 2226 2325
Size: 446 99
Fragments arranged by size:
483 446 341 306 251 214 99 97
49 39
BsiEI CG_ry'CG
Cuts at: 0 720 1468 2325
Size: 720 748 857
Fragments arranged by size:
857 748 720
BsiHKAI G_wGCw'C
Cuts at: 0 896 995 1859 2325
Size: 896 99 864 466
Fragments arranged by size:
896 864 466 99
BsmI GAATG_Cn'
Cuts at: 0 845 2325
Size: 845 1480
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1525 2325
Size: 1525 800
Bsp1286I G_dGCh'C
Cuts at: 0 44 428 477 710 896 919 963 995
Size: 44 384 49 233 186 23 44 32
Cuts at: 995 1499 1740 1859 1976 2199 2325
Size: 504 241 119 117 223 126
Fragments arranged by size:
504 384 241 233 223 186 126 119
117 49 44 44 32 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 536 557 2325
Size: 536 21 1768
Fragments arranged by size:
1768 536 21
BsrBI CCG'CTC
Cuts at: 0 205 1722 2325
Size: 205 1517 603
Fragments arranged by size:
1517 603 205
BsrFI r'CCGG_y
Cuts at: 0 179 226 428 680 1433 2172 2325
Size: 179 47 202 252 753 739 153
Fragments arranged by size:
753 739 252 202 179 153 47
BssHII G'CGCG_C
Cuts at: 0 175 817 2325
Size: 175 642 1508
Fragments arranged by size:
1508 642 175
BssSI C'ACGA_G
Cuts at: 0 883 2325
Size: 883 1442
BstAPI GCAn_nnn'nTGC
Cuts at: 0 354 774 2157 2325
Size: 354 420 1383 168
Fragments arranged by size:
1383 420 354 168
BstEII G'GTnAC_C
Cuts at: 0 877 2325
Size: 877 1448
BstXI CCAn_nnnn'nTGG
Cuts at: 0 864 1092 2325
Size: 864 228 1233
Fragments arranged by size:
1233 864 228
BstYI r'GATC_y
Cuts at: 0 1052 1073 1484 1794 2325
Size: 1052 21 411 310 531
Fragments arranged by size:
1052 531 411 310 21
BtgI C'CryG_G
Cuts at: 0 44 50 729 1127 1747 2061 2325
Size: 44 6 679 398 620 314 264
Fragments arranged by size:
679 620 398 314 264 44 6
BtsI GCAGTG_nn'
Cuts at: 0 497 1237 1589 1780 2325
Size: 497 740 352 191 545
Fragments arranged by size:
740 545 497 352 191
DraIII CAC_nnn'GTG
Cuts at: 0 978 1243 2325
Size: 978 265 1082
Fragments arranged by size:
1082 978 265
EaeI y'GGCC_r
Cuts at: 0 47 288 404 639 717 1094 1265 1465
Size: 47 241 116 235 78 377 171 200
Cuts at: 1465 1838 1998 2285 2325
Size: 373 160 287 40
Fragments arranged by size:
377 373 287 241 235 200 171 160
116 78 47 40
EagI C'GGCC_G
Cuts at: 0 717 1465 2325
Size: 717 748 860
Fragments arranged by size:
860 748 717
EarI CTCTTCn'nnn_
Cuts at: 0 2087 2325
Size: 2087 238
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 648 716 2325
Size: 648 68 1609
Fragments arranged by size:
1609 648 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 454 574 928 1312 1426 1621 1738
Size: 155 299 120 354 384 114 195 117
Cuts at: 1738 2125 2127 2230 2325
Size: 387 2 103 95
Fragments arranged by size:
387 384 354 299 195 155 120 117
114 103 95 2
EcoO109I rG'GnC_Cy
Cuts at: 0 40 314 474 942 1966 1973 2226 2262
Size: 40 274 160 468 1024 7 253 36
Cuts at: 2262 2325
Size: 63
Fragments arranged by size:
1024 468 274 253 160 63 40 36
7
FseI GG_CCGG'CC
Cuts at: 0 684 2325
Size: 684 1641
HaeII r_GCGC'y
Cuts at: 0 239 541 2070 2179 2190 2325
Size: 239 302 1529 109 11 135
Fragments arranged by size:
1529 302 239 135 109 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1786 1818 2325
Size: 1786 32 507
Fragments arranged by size:
1786 507 32
HindIII A'AGCT_T
Cuts at: 0 1013 2325
Size: 1013 1312
KasI G'GCGC_C
Cuts at: 0 235 2066 2325
Size: 235 1831 259
Fragments arranged by size:
1831 259 235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2069 2325
Size: 2069 256
MscI TGG'CCA
Cuts at: 0 641 2287 2325
Size: 641 1646 38
Fragments arranged by size:
1646 641 38
MslI CAynn'nnrTG
Cuts at: 0 97 490 862 1679 2325
Size: 97 393 372 817 646
Fragments arranged by size:
817 646 393 372 97
MspA1I CmG'CkG
Cuts at: 0 1005 1117 1232 1341 1404 1458 1470 1818
Size: 1005 112 115 109 63 54 12 348
Cuts at: 1818 1880 1948 2240 2283 2325
Size: 62 68 292 43 42
Fragments arranged by size:
1005 348 292 115 112 109 68 63
62 54 43 42 12
NaeI GCC'GGC
Cuts at: 0 181 228 682 1435 2174 2325
Size: 181 47 454 753 739 151
Fragments arranged by size:
753 739 454 181 151 47
NarI GG'CG_CC
Cuts at: 0 236 2067 2325
Size: 236 1831 258
Fragments arranged by size:
1831 258 236
NcoI C'CATG_G
Cuts at: 0 1747 2325
Size: 1747 578
NgoMIV G'CCGG_C
Cuts at: 0 179 226 680 1433 2172 2325
Size: 179 47 454 753 739 153
Fragments arranged by size:
753 739 454 179 153 47
NsiI A_TGCA'T
Cuts at: 0 1892 1928 2325
Size: 1892 36 397
Fragments arranged by size:
1892 397 36
NspI r_CATG'y
Cuts at: 0 762 999 1890 1894 2155 2325
Size: 762 237 891 4 261 170
Fragments arranged by size:
891 762 261 237 170 4
PasI CC'CwG_GG
Cuts at: 0 63 919 1422 2325
Size: 63 856 503 903
Fragments arranged by size:
903 856 503 63
PciI A'CATG_T
Cuts at: 0 995 2325
Size: 995 1330
PflMI CCAn_nnn'nTGG
Cuts at: 0 552 1243 1668 2325
Size: 552 691 425 657
Fragments arranged by size:
691 657 552 425
PfoI T'CCnGG_A
Cuts at: 0 1055 2325
Size: 1055 1270
PmlI CAC'GTG
Cuts at: 0 1707 2325
Size: 1707 618
PpuMI rG'GwC_Cy
Cuts at: 0 314 942 2226 2325
Size: 314 628 1284 99
Fragments arranged by size:
1284 628 314 99
PspOMI G'GGCC_C
Cuts at: 0 40 473 1972 2195 2325
Size: 40 433 1499 223 130
Fragments arranged by size:
1499 433 223 130 40
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1291 1323 2113 2145 2325
Size: 1291 32 790 32 180
Fragments arranged by size:
1291 790 180 32 32
PstI C_TGCA'G
Cuts at: 0 1596 2186 2325
Size: 1596 590 139
Fragments arranged by size:
1596 590 139
PvuII CAG'CTG
Cuts at: 0 1005 1117 1341 1404 1458 1948 2283 2325
Size: 1005 112 224 63 54 490 335 42
Fragments arranged by size:
1005 490 335 224 112 63 54 42
SanDI GG'GwC_CC
Cuts at: 0 942 2325
Size: 942 1383
SfcI C'TryA_G
Cuts at: 0 897 1592 2182 2325
Size: 897 695 590 143
Fragments arranged by size:
897 695 590 143
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 39 2325
Size: 39 2286
SfoI GGC'GCC
Cuts at: 0 237 2068 2325
Size: 237 1831 257
Fragments arranged by size:
1831 257 237
SmaI CCC'GGG
Cuts at: 0 222 1375 1741 2325
Size: 222 1153 366 584
Fragments arranged by size:
1153 584 366 222
SmlI C'TyrA_G
Cuts at: 0 2257 2325
Size: 2257 68
SphI G_CATG'C
Cuts at: 0 762 1890 1894 2325
Size: 762 1128 4 431
Fragments arranged by size:
1128 762 431 4
StuI AGG'CCT
Cuts at: 0 1513 1582 1912 2029 2325
Size: 1513 69 330 117 296
Fragments arranged by size:
1513 330 296 117 69
StyI C'CwwG_G
Cuts at: 0 1747 2325
Size: 1747 578
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 87 1958 2325
Size: 87 1871 367
Fragments arranged by size:
1871 367 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 94 126 1051 1083 1192 1224 2325
Size: 94 32 925 32 109 32 1101
Fragments arranged by size:
1101 925 109 94 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 808 2325
Size: 808 1517
XbaI T'CTAG_A
Cuts at: 0 1287 2325
Size: 1287 1038
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 399 736 1596 2325
Size: 399 337 860 729
Fragments arranged by size:
860 729 399 337
XmaI C'CCGG_G
Cuts at: 0 220 1373 1739 2325
Size: 220 1153 366 586
Fragments arranged by size:
1153 586 366 220
Enzymes that do cut:
AarI AfeI AflIII AleI AlfI AloI AlwNI ApaI
ApaLI AscI AvaI BaeI BanI BanII BbeI BbsI
BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI
BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoO109I
FseI HaeII Hin4I HindIII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmlI PpuMI PspOMI PsrI PstI
PvuII SanDI SfcI SfiI SfoI SmaI SmlI SphI
StuI StyI TaqII TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AhdI
ApoI AseI AsiSI AvrII BamHI BciVI BclI BmrI
BmtI BplI BsaI BsaBI BsaWI BsiWI BspEI BspHI
BsrDI BsrGI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EcoICRI EcoNI EcoRI EcoRV FalI FspI
FspAI HincII HpaI KpnI MfeI MluI NdeI NheI
NotI NruI PacI PmeI PpiI PshAI PsiI PspXI
PvuI RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TatI XhoI XmnI ZraI