Restriction map for
pFN21AE2571
(Linear) (Six-base) MAPSORT of: KIEE2571.seq Check: 1020 from: 1 to: 3978
KIEE2571.orf
With 182 enzymes: SgfI *
April 14, 2015 09:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 343 3978
Size: 343 3635
Acc65I G'GTAC_C
Cuts at: 0 2844 3978
Size: 2844 1134
AclI AA'CG_TT
Cuts at: 0 2194 2676 3978
Size: 2194 482 1302
Fragments arranged by size:
2194 1302 482
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 2948 3764 3978
Size: 186 2762 816 214
Fragments arranged by size:
2762 816 214 186
AfeI AGC'GCT
Cuts at: 0 2834 3978
Size: 2834 1144
AflIII A'CryG_T
Cuts at: 0 1067 3978
Size: 1067 2911
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3514 3978
Size: 3514 464
AlwNI CAG_nnn'CTG
Cuts at: 0 429 1388 3978
Size: 429 959 2590
Fragments arranged by size:
2590 959 429
ApaI G_GGCC'C
Cuts at: 0 463 3978
Size: 463 3515
ApoI r'AATT_y
Cuts at: 0 74 582 930 1554 2205 3082 3978
Size: 74 508 348 624 651 877 896
Fragments arranged by size:
896 877 651 624 508 348 74
AvaI C'yCGr_G
Cuts at: 0 190 298 2901 3978
Size: 190 108 2603 1077
Fragments arranged by size:
2603 1077 190 108
BanI G'GyrC_C
Cuts at: 0 331 2844 3978
Size: 331 2513 1134
Fragments arranged by size:
2513 1134 331
BanII G_rGCy'C
Cuts at: 0 16 463 1155 1319 2902 3607 3628 3978
Size: 16 447 692 164 1583 705 21 350
Fragments arranged by size:
1583 705 692 447 350 164 21 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 1200 1958 2867 3978
Size: 1200 758 909 1111
Fragments arranged by size:
1200 1111 909 758
BbvCI CC'TCA_GC
Cuts at: 0 2657 3978
Size: 2657 1321
BciVI GTATCCnnnnn_n'
Cuts at: 0 540 2724 2779 3978
Size: 540 2184 55 1199
Fragments arranged by size:
2184 1199 540 55
BglI GCCn_nnn'nGGC
Cuts at: 0 3923 3978
Size: 3923 55
BglII A'GATC_T
Cuts at: 0 710 2724 3978
Size: 710 2014 1254
Fragments arranged by size:
2014 1254 710
BlpI GC'TnA_GC
Cuts at: 0 452 3978
Size: 452 3526
Bme1580I G_kGCm'C
Cuts at: 0 463 3978
Size: 463 3515
BmgBI CAC'GTC
Cuts at: 0 306 2611 3978
Size: 306 2305 1367
Fragments arranged by size:
2305 1367 306
BmrI ACTGGGnnnn_n'
Cuts at: 0 3650 3978
Size: 3650 328
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 30 1075 1174 2940 3978
Size: 30 1045 99 1766 1038
Fragments arranged by size:
1766 1045 1038 99 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 406 883 1182 2312 3576 3888 3978
Size: 350 56 477 299 1130 1264 312 90
Fragments arranged by size:
1264 1130 477 350 312 299 90 56
Bpu10I CC'TnA_GC
Cuts at: 0 1313 1801 2657 3978
Size: 1313 488 856 1321
Fragments arranged by size:
1321 1313 856 488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 735 1397 1651 1904 2356 3978
Size: 735 662 254 253 452 1622
Fragments arranged by size:
1622 735 662 452 254 253
BsaI GGTCTCn'nnnn_
Cuts at: 0 2266 3055 3602 3978
Size: 2266 789 547 376
Fragments arranged by size:
2266 789 547 376
BsaAI yAC'GTr
Cuts at: 0 2443 3978
Size: 2443 1535
BsaBI GATnn'nnATC
Cuts at: 0 1282 3917 3978
Size: 1282 2635 61
Fragments arranged by size:
2635 1282 61
BsaHI Gr'CG_yC
Cuts at: 0 224 292 3978
Size: 224 68 3686
Fragments arranged by size:
3686 224 68
BsaWI w'CCGG_w
Cuts at: 0 1217 3978
Size: 1217 2761
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 104 134 3978
Size: 104 30 3844
Fragments arranged by size:
3844 104 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 2674 2710 3179 3267 3385 3978
Size: 22 2652 36 469 88 118 593
Fragments arranged by size:
2652 593 469 118 88 36 22
BseYI C'CCAG_C
Cuts at: 0 1085 2060 3812 3817 3857 3978
Size: 1085 975 1752 5 40 121
Fragments arranged by size:
1752 1085 975 121 40 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 1820 3978
Size: 291 1529 2158
Fragments arranged by size:
2158 1529 291
BsiHKAI G_wGCw'C
Cuts at: 0 16 807 1155 1190 1413 2902 3347 3607
Size: 16 791 348 35 223 1489 445 260
Cuts at: 3607 3978
Size: 371
Fragments arranged by size:
1489 791 445 371 348 260 223 35
16
BsmI GAATG_Cn'
Cuts at: 0 2786 3978
Size: 2786 1192
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2123 3978
Size: 2123 1855
Bsp1286I G_dGCh'C
Cuts at: 0 16 463 807 1155 1190 1319 1413 2902
Size: 16 447 344 348 35 129 94 1489
Cuts at: 2902 3347 3607 3628 3978
Size: 445 260 21 350
Fragments arranged by size:
1489 447 445 350 348 344 260 129
94 35 21 16
BspHI T'CATG_A
Cuts at: 0 377 3978
Size: 377 3601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 343 1988 3978
Size: 343 1645 1990
Fragments arranged by size:
1990 1645 343
BsrBI CCG'CTC
Cuts at: 0 2163 3978
Size: 2163 1815
BsrDI GCAATG_nn'
Cuts at: 0 629 1430 2574 3978
Size: 629 801 1144 1404
Fragments arranged by size:
1404 1144 801 629
BssSI C'ACGA_G
Cuts at: 0 1993 3978
Size: 1993 1985
BstEII G'GTnAC_C
Cuts at: 0 1677 3099 3978
Size: 1677 1422 879
Fragments arranged by size:
1677 1422 879
BstXI CCAn_nnnn'nTGG
Cuts at: 0 288 741 2221 3978
Size: 288 453 1480 1757
Fragments arranged by size:
1757 1480 453 288
BstYI r'GATC_y
Cuts at: 0 678 710 1377 2724 3978
Size: 678 32 667 1347 1254
Fragments arranged by size:
1347 1254 678 667 32
Bsu36I CC'TnA_GG
Cuts at: 0 340 3978
Size: 340 3638
BtgI C'CryG_G
Cuts at: 0 392 446 1161 2342 3978
Size: 392 54 715 1181 1636
Fragments arranged by size:
1636 1181 715 392 54
BtsI GCAGTG_nn'
Cuts at: 0 1780 1832 3498 3978
Size: 1780 52 1666 480
Fragments arranged by size:
1780 1666 480 52
DraI TTT'AAA
Cuts at: 0 2150 3978
Size: 2150 1828
DraIII CAC_nnn'GTG
Cuts at: 0 988 3978
Size: 988 2990
EaeI y'GGCC_r
Cuts at: 0 797 1772 3798 3915 3978
Size: 797 975 2026 117 63
Fragments arranged by size:
2026 975 797 117 63
EarI CTCTTCn'nnn_
Cuts at: 0 1160 2758 2834 2924 3392 3978
Size: 1160 1598 76 90 468 586
Fragments arranged by size:
1598 1160 586 468 90 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3480 3978
Size: 3480 498
EcoICRI GAG'CTC
Cuts at: 0 14 1153 2900 3605 3978
Size: 14 1139 1747 705 373
Fragments arranged by size:
1747 1139 705 373 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 350 406 883 1182 2312 2948 3576
Size: 186 164 56 477 299 1130 636 628
Cuts at: 3576 3764 3888 3978
Size: 188 124 90
Fragments arranged by size:
1130 636 628 477 299 188 186 164
124 90 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 339 2035 2884 3978
Size: 339 1696 849 1094
Fragments arranged by size:
1696 1094 849 339
EcoO109I rG'GnC_Cy
Cuts at: 0 459 460 615 3978
Size: 459 1 155 3363
Fragments arranged by size:
3363 459 155 1
EcoRV GAT'ATC
Cuts at: 0 2487 3978
Size: 2487 1491
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3766 3978
Size: 3766 212
HaeII r_GCGC'y
Cuts at: 0 411 430 2836 3978
Size: 411 19 2406 1142
Fragments arranged by size:
2406 1142 411 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1045 1077 1384 1416 1612 1644 2120 2137
Size: 1045 32 307 32 196 32 476 17
Cuts at: 2137 2152 2169 2554 2586 2610 2642 3715 3747
Size: 15 17 385 32 24 32 1073 32
Cuts at: 3747 3978
Size: 231
Fragments arranged by size:
1073 1045 476 385 307 231 196 32
32 32 32 32 32 24 17 17
15
HincII GTy'rAC
Cuts at: 0 197 3128 3978
Size: 197 2931 850
Fragments arranged by size:
2931 850 197
HpaI GTT'AAC
Cuts at: 0 197 3978
Size: 197 3781
KpnI G_GTAC'C
Cuts at: 0 2848 3978
Size: 2848 1130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 737 2447 3978
Size: 80 657 1710 1531
Fragments arranged by size:
1710 1531 657 80
MscI TGG'CCA
Cuts at: 0 799 1774 3800 3917 3978
Size: 799 975 2026 117 61
Fragments arranged by size:
2026 975 799 117 61
MslI CAynn'nnrTG
Cuts at: 0 280 286 2219 2689 3978
Size: 280 6 1933 470 1289
Fragments arranged by size:
1933 1289 470 280 6
MspA1I CmG'CkG
Cuts at: 0 3004 3978
Size: 3004 974
NcoI C'CATG_G
Cuts at: 0 392 2342 3978
Size: 392 1950 1636
Fragments arranged by size:
1950 1636 392
NspI r_CATG'y
Cuts at: 0 1071 3978
Size: 1071 2907
PasI CC'CwG_GG
Cuts at: 0 26 1698 3978
Size: 26 1672 2280
Fragments arranged by size:
2280 1672 26
PciI A'CATG_T
Cuts at: 0 1067 3978
Size: 1067 2911
PflMI CCAn_nnn'nTGG
Cuts at: 0 447 3122 3486 3978
Size: 447 2675 364 492
Fragments arranged by size:
2675 492 447 364
PfoI T'CCnGG_A
Cuts at: 0 2138 2558 3978
Size: 2138 420 1420
Fragments arranged by size:
2138 1420 420
PpuMI rG'GwC_Cy
Cuts at: 0 615 3978
Size: 615 3363
PshAI GACnn'nnGTC
Cuts at: 0 1675 3978
Size: 1675 2303
PspOMI G'GGCC_C
Cuts at: 0 459 3978
Size: 459 3519
PspXI vC'TCGA_Gb
Cuts at: 0 190 3978
Size: 190 3788
PstI C_TGCA'G
Cuts at: 0 1640 1787 2036 3644 3978
Size: 1640 147 249 1608 334
Fragments arranged by size:
1640 1608 334 249 147
SacI G_AGCT'C
Cuts at: 0 16 1155 2902 3607 3978
Size: 16 1139 1747 705 371
Fragments arranged by size:
1747 1139 705 371 16
SapI GCTCTTCn'nnn_
Cuts at: 0 1160 3978
Size: 1160 2818
SfcI C'TryA_G
Cuts at: 0 1636 1783 2032 3640 3978
Size: 1636 147 249 1608 338
Fragments arranged by size:
1636 1608 338 249 147
SmlI C'TyrA_G
Cuts at: 0 190 714 1412 1666 1919 2335 3978
Size: 190 524 698 254 253 416 1643
Fragments arranged by size:
1643 698 524 416 254 253 190
SspI AAT'ATT
Cuts at: 0 1177 3978
Size: 1177 2801
StuI AGG'CCT
Cuts at: 0 2023 3978
Size: 2023 1955
StyI C'CwwG_G
Cuts at: 0 392 2342 3978
Size: 392 1950 1636
Fragments arranged by size:
1950 1636 392
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 2074 3010 3978
Size: 19 2055 936 968
Fragments arranged by size:
2055 968 936 19
TatI w'GTAC_w
Cuts at: 0 531 1605 2091 3978
Size: 531 1074 486 1887
Fragments arranged by size:
1887 1074 531 486
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3186 3218 3978
Size: 3186 32 760
Fragments arranged by size:
3186 760 32
Tth111I GACn'n_nGTC
Cuts at: 0 1141 3978
Size: 1141 2837
XbaI T'CTAG_A
Cuts at: 0 2823 3978
Size: 2823 1155
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1104 2688 3657 3978
Size: 1104 1584 969 321
Fragments arranged by size:
1584 1104 969 321
XhoI C'TCGA_G
Cuts at: 0 190 3978
Size: 190 3788
Enzymes that do cut:
AarI Acc65I AclI AcuI AfeI AflIII AlfI AlwNI
ApaI ApoI AvaI BanI BanII BbsI BbvCI BciVI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BssSI BstEII BstXI BstYI Bsu36I
BtgI BtsI DraI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII
HpaI KpnI MmeI MscI MslI MspA1I NcoI NspI
PasI PciI PflMI PfoI PpuMI PshAI PspOMI PspXI
PstI SacI SapI SfcI SmlI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI
Enzymes that do not cut:
AatII AccI AflII AgeI AhdI AleI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI
BclI BfrBI BmtI BsiEI BsiWI BspEI BsrFI BsrGI
BssHII BstAPI BstBI BstZ17I BtgZI ClaI CspCI DrdI
EagI EcoRI FseI FspI FspAI HindIII KasI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PmeI PmlI PpiI PsiI PsrI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI XmaI XmnI ZraI