Restriction map for
pFN21AE2565
(Linear) (Six-base) MAPSORT of: KIEE2565.seq Check: 2302 from: 1 to: 3456
KIEE2565.orf
With 182 enzymes: SgfI *
March 23, 2015 09:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 971 1970 2216 2815 3344 3362 3456
Size: 754 217 999 246 599 529 18 94
Fragments arranged by size:
999 754 599 529 246 217 94 18
AflIII A'CryG_T
Cuts at: 0 1514 3456
Size: 1514 1942
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1865 3456
Size: 1865 1591
AlwNI CAG_nnn'CTG
Cuts at: 0 622 2377 2440 3340 3456
Size: 622 1755 63 900 116
Fragments arranged by size:
1755 900 622 116 63
ApaLI G'TGCA_C
Cuts at: 0 2948 3456
Size: 2948 508
ApoI r'AATT_y
Cuts at: 0 304 3456
Size: 304 3152
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 965 998 3456
Size: 965 33 2458
Fragments arranged by size:
2458 965 33
BamHI G'GATC_C
Cuts at: 0 3214 3456
Size: 3214 242
BanI G'GyrC_C
Cuts at: 0 750 3349 3456
Size: 750 2599 107
Fragments arranged by size:
2599 750 107
BanII G_rGCy'C
Cuts at: 0 2078 2476 3456
Size: 2078 398 980
Fragments arranged by size:
2078 980 398
BbeI G_GCGC'C
Cuts at: 0 3353 3456
Size: 3353 103
BbsI GAAGACnn'nnnn_
Cuts at: 0 742 3265 3456
Size: 742 2523 191
Fragments arranged by size:
2523 742 191
BbvCI CC'TCA_GC
Cuts at: 0 638 3456
Size: 638 2818
BfrBI ATG'CAT
Cuts at: 0 25 3456
Size: 25 3431
BglI GCCn_nnn'nGGC
Cuts at: 0 2688 3377 3456
Size: 2688 689 79
Fragments arranged by size:
2688 689 79
BglII A'GATC_T
Cuts at: 0 2392 3456
Size: 2392 1064
BlpI GC'TnA_GC
Cuts at: 0 2758 3456
Size: 2758 698
Bme1580I G_kGCm'C
Cuts at: 0 2952 3456
Size: 2952 504
BmgBI CAC'GTC
Cuts at: 0 2310 3456
Size: 2310 1146
BmrI ACTGGGnnnn_n'
Cuts at: 0 1414 3456
Size: 1414 2042
BmtI G_CTAG'C
Cuts at: 0 667 3456
Size: 667 2789
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1088 1166 3456
Size: 1088 78 2290
Fragments arranged by size:
2290 1088 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 450 1553 1595 1697 2373 2867 3074
Size: 26 424 1103 42 102 676 494 207
Cuts at: 3074 3456
Size: 382
Fragments arranged by size:
1103 676 494 424 382 207 102 42
26
Bpu10I CC'TnA_GC
Cuts at: 0 386 638 1676 2174 3456
Size: 386 252 1038 498 1282
Fragments arranged by size:
1282 1038 498 386 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2592 3456
Size: 2592 864
BsaI GGTCTCn'nnnn_
Cuts at: 0 299 1984 3108 3456
Size: 299 1685 1124 348
Fragments arranged by size:
1685 1124 348 299
BsaHI Gr'CG_yC
Cuts at: 0 3350 3456
Size: 3350 106
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 107 137 230 260 279 309 1551 1581
Size: 107 30 93 30 19 30 1242 30
Cuts at: 1581 2769 2799 3143 3173 3239 3269 3456
Size: 1188 30 344 30 66 30 187
Fragments arranged by size:
1242 1188 344 187 107 93 66 30
30 30 30 30 30 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 626 1933 2864 3096 3167 3275 3328 3456
Size: 626 1307 931 232 71 108 53 128
Fragments arranged by size:
1307 931 626 232 128 108 71 53
BseYI C'CCAG_C
Cuts at: 0 1029 1215 1647 2382 2656 2737 3307 3456
Size: 1029 186 432 735 274 81 570 149
Fragments arranged by size:
1029 735 570 432 274 186 149 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1835 1923 3456
Size: 1835 88 1533
Fragments arranged by size:
1835 1533 88
BsiEI CG_ry'CG
Cuts at: 0 1916 3456
Size: 1916 1540
BsiHKAI G_wGCw'C
Cuts at: 0 202 2078 2410 2590 2952 3456
Size: 202 1876 332 180 362 504
Fragments arranged by size:
1876 504 362 332 202 180
Bsp1286I G_dGCh'C
Cuts at: 0 202 2078 2410 2476 2590 2952 3456
Size: 202 1876 332 66 114 362 504
Fragments arranged by size:
1876 504 362 332 202 114 66
BspHI T'CATG_A
Cuts at: 0 1862 3456
Size: 1862 1594
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2133 2535 2970 3249 3456
Size: 2133 402 435 279 207
Fragments arranged by size:
2133 435 402 279 207
BsrBI CCG'CTC
Cuts at: 0 2807 3456
Size: 2807 649
BsrDI GCAATG_nn'
Cuts at: 0 74 2324 3456
Size: 74 2250 1132
Fragments arranged by size:
2250 1132 74
BsrFI r'CCGG_y
Cuts at: 0 1911 2932 3318 3456
Size: 1911 1021 386 138
Fragments arranged by size:
1911 1021 386 138
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3250 3456
Size: 3250 206
BstEII G'GTnAC_C
Cuts at: 0 3403 3456
Size: 3403 53
BstXI CCAn_nnnn'nTGG
Cuts at: 0 789 999 1107 1185 1227 1473 3456
Size: 789 210 108 78 42 246 1983
Fragments arranged by size:
1983 789 246 210 108 78 42
BstYI r'GATC_y
Cuts at: 0 2392 3214 3437 3456
Size: 2392 822 223 19
Fragments arranged by size:
2392 822 223 19
Bsu36I CC'TnA_GG
Cuts at: 0 560 1995 3456
Size: 560 1435 1461
Fragments arranged by size:
1461 1435 560
BtgI C'CryG_G
Cuts at: 0 884 2822 3456
Size: 884 1938 634
Fragments arranged by size:
1938 884 634
BtsI GCAGTG_nn'
Cuts at: 0 1351 2501 3456
Size: 1351 1150 955
Fragments arranged by size:
1351 1150 955
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2178 2213 3129 3164 3456
Size: 2178 35 916 35 292
Fragments arranged by size:
2178 916 292 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2553 3456
Size: 2553 903
EaeI y'GGCC_r
Cuts at: 0 355 863 1337 1463 1697 1913 2111 2729
Size: 355 508 474 126 234 216 198 618
Cuts at: 2729 2768 3320 3378 3456
Size: 39 552 58 78
Fragments arranged by size:
618 552 508 474 355 234 216 198
126 78 58 39
EagI C'GGCC_G
Cuts at: 0 1913 3456
Size: 1913 1543
EarI CTCTTCn'nnn_
Cuts at: 0 308 686 3456
Size: 308 378 2770
Fragments arranged by size:
2770 378 308
EcoICRI GAG'CTC
Cuts at: 0 2076 3456
Size: 2076 1380
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 450 754 971 1553 1595 1697 1970
Size: 26 424 304 217 582 42 102 273
Cuts at: 1970 2216 2373 2815 2867 3074 3344 3362 3456
Size: 246 157 442 52 207 270 18 94
Fragments arranged by size:
582 442 424 304 273 270 246 217
207 157 102 94 52 42 26 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 781 907 949 1633 1675 2280 2849 3149
Size: 781 126 42 684 42 605 569 300
Cuts at: 3149 3245 3456
Size: 96 211
Fragments arranged by size:
781 684 605 569 300 211 126 96
42 42
EcoO109I rG'GnC_Cy
Cuts at: 0 1644 3298 3456
Size: 1644 1654 158
Fragments arranged by size:
1654 1644 158
EcoRI G'AATT_C
Cuts at: 0 304 3456
Size: 304 3152
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 87 1923 3201 3456
Size: 87 1836 1278 255
Fragments arranged by size:
1836 1278 255 87
HaeII r_GCGC'y
Cuts at: 0 3340 3353 3456
Size: 3340 13 103
Fragments arranged by size:
3340 103 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 214 246 820 852 1054 1086 1132 1164
Size: 214 32 574 32 202 32 46 32
Cuts at: 1164 1258 1290 1551 1583 1623 1655 3143 3156
Size: 94 32 261 32 40 32 1488 13
Cuts at: 3156 3175 3188 3456
Size: 19 13 268
Fragments arranged by size:
1488 574 268 261 214 202 94 46
40 32 32 32 32 32 32 32
19 13 13
HincII GTy'rAC
Cuts at: 0 1969 3456
Size: 1969 1487
KasI G'GCGC_C
Cuts at: 0 3349 3456
Size: 3349 107
MfeI C'AATT_G
Cuts at: 0 2939 3456
Size: 2939 517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 322 405 3456
Size: 105 217 83 3051
Fragments arranged by size:
3051 217 105 83
MscI TGG'CCA
Cuts at: 0 357 865 1339 1699 2113 2770 3380 3456
Size: 357 508 474 360 414 657 610 76
Fragments arranged by size:
657 610 508 474 414 360 357 76
MslI CAynn'nnrTG
Cuts at: 0 437 2677 3456
Size: 437 2240 779
Fragments arranged by size:
2240 779 437
MspA1I CmG'CkG
Cuts at: 0 478 550 1849 2248 2437 2566 2641 2887
Size: 478 72 1299 399 189 129 75 246
Cuts at: 2887 3456
Size: 569
Fragments arranged by size:
1299 569 478 399 246 189 129 75
72
NaeI GCC'GGC
Cuts at: 0 1913 2934 3320 3456
Size: 1913 1021 386 136
Fragments arranged by size:
1913 1021 386 136
NarI GG'CG_CC
Cuts at: 0 3350 3456
Size: 3350 106
NcoI C'CATG_G
Cuts at: 0 884 3456
Size: 884 2572
NgoMIV G'CCGG_C
Cuts at: 0 1911 2932 3318 3456
Size: 1911 1021 386 138
Fragments arranged by size:
1911 1021 386 138
NheI G'CTAG_C
Cuts at: 0 663 3456
Size: 663 2793
NsiI A_TGCA'T
Cuts at: 0 27 3456
Size: 27 3429
NspI r_CATG'y
Cuts at: 0 876 1518 3049 3456
Size: 876 642 1531 407
Fragments arranged by size:
1531 876 642 407
PciI A'CATG_T
Cuts at: 0 1514 3456
Size: 1514 1942
PflMI CCAn_nnn'nTGG
Cuts at: 0 3426 3456
Size: 3426 30
PfoI T'CCnGG_A
Cuts at: 0 149 3440 3456
Size: 149 3291 16
Fragments arranged by size:
3291 149 16
PpuMI rG'GwC_Cy
Cuts at: 0 1644 3298 3456
Size: 1644 1654 158
Fragments arranged by size:
1654 1644 158
PshAI GACnn'nnGTC
Cuts at: 0 11 2573 3456
Size: 11 2562 883
Fragments arranged by size:
2562 883 11
PstI C_TGCA'G
Cuts at: 0 1832 2532 2646 3246 3456
Size: 1832 700 114 600 210
Fragments arranged by size:
1832 700 600 210 114
PvuII CAG'CTG
Cuts at: 0 478 550 1849 2248 2437 2566 2887 3456
Size: 478 72 1299 399 189 129 321 569
Fragments arranged by size:
1299 569 478 399 321 189 129 72
SacI G_AGCT'C
Cuts at: 0 2078 3456
Size: 2078 1378
SanDI GG'GwC_CC
Cuts at: 0 3298 3456
Size: 3298 158
SbfI CC_TGCA'GG
Cuts at: 0 3246 3456
Size: 3246 210
SfcI C'TryA_G
Cuts at: 0 287 1828 2528 2642 3000 3242 3456
Size: 287 1541 700 114 358 242 214
Fragments arranged by size:
1541 700 358 287 242 214 114
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3377 3456
Size: 3377 79
SfoI GGC'GCC
Cuts at: 0 3351 3456
Size: 3351 105
SmlI C'TyrA_G
Cuts at: 0 2571 3456
Size: 2571 885
SspI AAT'ATT
Cuts at: 0 491 3039 3456
Size: 491 2548 417
Fragments arranged by size:
2548 491 417
StuI AGG'CCT
Cuts at: 0 97 2025 2286 3456
Size: 97 1928 261 1170
Fragments arranged by size:
1928 1170 261 97
StyI C'CwwG_G
Cuts at: 0 668 782 866 884 1034 1100 1178 1220
Size: 668 114 84 18 150 66 78 42
Cuts at: 1220 1382 1466 2810 3029 3206 3425 3456
Size: 162 84 1344 219 177 219 31
Fragments arranged by size:
1344 668 219 219 177 162 150 114
84 84 78 66 42 31 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 855 1131 1209 2271 2691 3456
Size: 855 276 78 1062 420 765
Fragments arranged by size:
1062 855 765 420 276 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 586 618 2012 2044 3239 3271 3456
Size: 586 32 1394 32 1195 32 185
Fragments arranged by size:
1394 1195 586 185 32 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 1583 3456
Size: 1583 1873
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaLI ApoI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII KasI MfeI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI
NspI PciI PflMI PfoI PpuMI PshAI PstI PvuII
SacI SanDI SbfI SfcI SfiI SfoI SmlI SspI
StuI StyI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvaI
AvrII BcgI BciVI BclI BsaAI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BsrGI BssHII BssSI BstBI BstZ17I
BtgZI ClaI DraI DraIII EciI EcoRV FseI FspI
FspAI HindIII HpaI KpnI MluI NdeI NotI NruI
PacI PasI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SapI ScaI SexAI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
TatI Tth111I XbaI XcmI XhoI XmaI ZraI