Restriction map for
pFN21AE2563
(Linear) (Six-base) MAPSORT of: KIEE2563.seq Check: 6749 from: 1 to: 2985
KIEE2563.orf
With 182 enzymes: SgfI *
March 4, 2015 11:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 89 603 2985
Size: 89 514 2382
Fragments arranged by size:
2382 514 89
AatII G_ACGT'C
Cuts at: 0 2033 2985
Size: 2033 952
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 2515 2985
Size: 709 1806 470
Fragments arranged by size:
1806 709 470
AfeI AGC'GCT
Cuts at: 0 1498 2985
Size: 1498 1487
AgeI A'CCGG_T
Cuts at: 0 928 2985
Size: 928 2057
AlwNI CAG_nnn'CTG
Cuts at: 0 1671 1715 1947 2696 2769 2985
Size: 1671 44 232 749 73 216
Fragments arranged by size:
1671 749 232 216 73 44
ApaI G_GGCC'C
Cuts at: 0 512 2971 2985
Size: 512 2459 14
Fragments arranged by size:
2459 512 14
ApoI r'AATT_y
Cuts at: 0 582 802 819 2985
Size: 582 220 17 2166
Fragments arranged by size:
2166 582 220 17
AvaI C'yCGr_G
Cuts at: 0 137 2985
Size: 137 2848
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1181 1214 2985
Size: 1181 33 1771
Fragments arranged by size:
1771 1181 33
BanI G'GyrC_C
Cuts at: 0 964 2985
Size: 964 2021
BanII G_rGCy'C
Cuts at: 0 459 512 614 977 1133 2971 2985
Size: 459 53 102 363 156 1838 14
Fragments arranged by size:
1838 459 363 156 102 53 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 1823 2985
Size: 1823 1162
BbvCI CC'TCA_GC
Cuts at: 0 2963 2985
Size: 2963 22
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1701 1735 2985
Size: 1701 34 1250
Fragments arranged by size:
1701 1250 34
BclI T'GATC_A
Cuts at: 0 500 2225 2985
Size: 500 1725 760
Fragments arranged by size:
1725 760 500
BfrBI ATG'CAT
Cuts at: 0 798 2985
Size: 798 2187
BglI GCCn_nnn'nGGC
Cuts at: 0 281 1392 1960 2985
Size: 281 1111 568 1025
Fragments arranged by size:
1111 1025 568 281
BglII A'GATC_T
Cuts at: 0 54 573 1062 2457 2630 2985
Size: 54 519 489 1395 173 355
Fragments arranged by size:
1395 519 489 355 173 54
BlpI GC'TnA_GC
Cuts at: 0 1483 2985
Size: 1483 1502
Bme1580I G_kGCm'C
Cuts at: 0 512 2971 2985
Size: 512 2459 14
Fragments arranged by size:
2459 512 14
BmgBI CAC'GTC
Cuts at: 0 1650 2985
Size: 1650 1335
BmrI ACTGGGnnnn_n'
Cuts at: 0 1283 2171 2268 2985
Size: 1283 888 97 717
Fragments arranged by size:
1283 888 717 97
BmtI G_CTAG'C
Cuts at: 0 2218 2985
Size: 2218 767
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 249 379 1554 2985
Size: 249 130 1175 1431
Fragments arranged by size:
1431 1175 249 130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 1477 2362 2380 2424 2985
Size: 562 915 885 18 44 561
Fragments arranged by size:
915 885 562 561 44 18
Bpu10I CC'TnA_GC
Cuts at: 0 214 1874 2207 2963 2985
Size: 214 1660 333 756 22
Fragments arranged by size:
1660 756 333 214 22
BsaI GGTCTCn'nnnn_
Cuts at: 0 110 1897 2985
Size: 110 1787 1088
Fragments arranged by size:
1787 1088 110
BsaAI yAC'GTr
Cuts at: 0 2310 2435 2985
Size: 2310 125 550
Fragments arranged by size:
2310 550 125
BsaHI Gr'CG_yC
Cuts at: 0 988 2030 2062 2985
Size: 988 1042 32 923
Fragments arranged by size:
1042 988 923 32
BsaWI w'CCGG_w
Cuts at: 0 928 2985
Size: 928 2057
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 517 547 915 945 2985
Size: 517 30 368 30 2040
Fragments arranged by size:
2040 517 368 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 155 2359 2985
Size: 155 2204 626
Fragments arranged by size:
2204 626 155
BseYI C'CCAG_C
Cuts at: 0 1443 2762 2985
Size: 1443 1319 223
Fragments arranged by size:
1443 1319 223
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1978 2611 2936 2985
Size: 1978 633 325 49
Fragments arranged by size:
1978 633 325 49
BsiEI CG_ry'CG
Cuts at: 0 1438 2985
Size: 1438 1547
BsiHKAI G_wGCw'C
Cuts at: 0 659 679 2440 2985
Size: 659 20 1761 545
Fragments arranged by size:
1761 659 545 20
BsmI GAATG_Cn'
Cuts at: 0 2239 2985
Size: 2239 746
Bsp1286I G_dGCh'C
Cuts at: 0 459 512 614 659 679 977 1133 2440
Size: 459 53 102 45 20 298 156 1307
Cuts at: 2440 2971 2985
Size: 531 14
Fragments arranged by size:
1307 531 459 298 156 102 53 45
20 14
BspHI T'CATG_A
Cuts at: 0 1185 1583 2985
Size: 1185 398 1402
Fragments arranged by size:
1402 1185 398
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 89 603 945 2985
Size: 89 514 342 2040
Fragments arranged by size:
2040 514 342 89
BsrDI GCAATG_nn'
Cuts at: 0 915 2957 2985
Size: 915 2042 28
Fragments arranged by size:
2042 915 28
BsrFI r'CCGG_y
Cuts at: 0 928 1437 2985
Size: 928 509 1548
Fragments arranged by size:
1548 928 509
BsrGI T'GTAC_A
Cuts at: 0 292 2985
Size: 292 2693
BssSI C'ACGA_G
Cuts at: 0 107 399 706 2135 2260 2985
Size: 107 292 307 1429 125 725
Fragments arranged by size:
1429 725 307 292 125 107
BstAPI GCAn_nnn'nTGC
Cuts at: 0 683 1715 1947 1956 2926 2985
Size: 683 1032 232 9 970 59
Fragments arranged by size:
1032 970 683 232 59 9
BstEII G'GTnAC_C
Cuts at: 0 155 2985
Size: 155 2830
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1296 2073 2985
Size: 1296 777 912
Fragments arranged by size:
1296 912 777
BstYI r'GATC_y
Cuts at: 0 54 573 1062 2303 2457 2630 2985
Size: 54 519 489 1241 154 173 355
Fragments arranged by size:
1241 519 489 355 173 154 54
Bsu36I CC'TnA_GG
Cuts at: 0 224 2985
Size: 224 2761
BtgI C'CryG_G
Cuts at: 0 459 2744 2985
Size: 459 2285 241
Fragments arranged by size:
2285 459 241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1433 2985
Size: 1433 1552
BtsI GCAGTG_nn'
Cuts at: 0 198 258 556 766 1504 2084 2599 2792
Size: 198 60 298 210 738 580 515 193
Cuts at: 2792 2985
Size: 193
Fragments arranged by size:
738 580 515 298 210 198 193 193
60
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 615 650 2246 2281 2985
Size: 615 35 1596 35 704
Fragments arranged by size:
1596 704 615 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1291 2985
Size: 1291 1694
DrdI GACnn_nn'nnGTC
Cuts at: 0 304 1621 2985
Size: 304 1317 1364
Fragments arranged by size:
1364 1317 304
EaeI y'GGCC_r
Cuts at: 0 1435 1472 1520 1979 2985
Size: 1435 37 48 459 1006
Fragments arranged by size:
1435 1006 459 48 37
EagI C'GGCC_G
Cuts at: 0 1435 2985
Size: 1435 1550
EarI CTCTTCn'nnn_
Cuts at: 0 1582 2042 2985
Size: 1582 460 943
Fragments arranged by size:
1582 943 460
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 498 1501 1855 2985
Size: 498 1003 354 1130
Fragments arranged by size:
1130 1003 498 354
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 709 1477 2362 2380 2424 2515 2985
Size: 562 147 768 885 18 44 91 470
Fragments arranged by size:
885 768 562 470 147 91 44 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1723 2985
Size: 1723 1262
EcoO109I rG'GnC_Cy
Cuts at: 0 741 957 1370 1866 1998 2181 2391 2693
Size: 741 216 413 496 132 183 210 302
Cuts at: 2693 2967 2985
Size: 274 18
Fragments arranged by size:
741 496 413 302 274 216 210 183
132 18
EcoRI G'AATT_C
Cuts at: 0 582 2985
Size: 582 2403
EcoRV GAT'ATC
Cuts at: 0 1702 2985
Size: 1702 1283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1105 1641 2985
Size: 1105 536 1344
Fragments arranged by size:
1344 1105 536
FseI GG_CCGG'CC
Cuts at: 0 1441 2985
Size: 1441 1544
HaeII r_GCGC'y
Cuts at: 0 1500 2568 2985
Size: 1500 1068 417
Fragments arranged by size:
1500 1068 417
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 517 549 941 973 1001 1033 1138 1170
Size: 517 32 392 32 28 32 105 32
Cuts at: 1170 1297 1329 2985
Size: 127 32 1656
Fragments arranged by size:
1656 517 392 127 105 32 32 32
32 32 28
HincII GTy'rAC
Cuts at: 0 1809 2037 2985
Size: 1809 228 948
Fragments arranged by size:
1809 948 228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 632 1258 1571 2985
Size: 632 626 313 1414
Fragments arranged by size:
1414 632 626 313
MslI CAynn'nnrTG
Cuts at: 0 172 1046 1103 1124 2227 2912 2985
Size: 172 874 57 21 1103 685 73
Fragments arranged by size:
1103 874 685 172 73 57 21
MspA1I CmG'CkG
Cuts at: 0 668 1419 1482 1944 2340 2493 2985
Size: 668 751 63 462 396 153 492
Fragments arranged by size:
751 668 492 462 396 153 63
NaeI GCC'GGC
Cuts at: 0 1439 2985
Size: 1439 1546
NgoMIV G'CCGG_C
Cuts at: 0 1437 2985
Size: 1437 1548
NheI G'CTAG_C
Cuts at: 0 2214 2985
Size: 2214 771
NsiI A_TGCA'T
Cuts at: 0 800 2985
Size: 800 2185
NspI r_CATG'y
Cuts at: 0 732 1123 2985
Size: 732 391 1862
Fragments arranged by size:
1862 732 391
PasI CC'CwG_GG
Cuts at: 0 942 2985
Size: 942 2043
PflMI CCAn_nnn'nTGG
Cuts at: 0 622 2769 2985
Size: 622 2147 216
Fragments arranged by size:
2147 622 216
PfoI T'CCnGG_A
Cuts at: 0 1862 2985
Size: 1862 1123
PmlI CAC'GTG
Cuts at: 0 2310 2435 2985
Size: 2310 125 550
Fragments arranged by size:
2310 550 125
PpuMI rG'GwC_Cy
Cuts at: 0 741 957 1866 1998 2181 2693 2985
Size: 741 216 909 132 183 512 292
Fragments arranged by size:
909 741 512 292 216 183 132
PspOMI G'GGCC_C
Cuts at: 0 508 2967 2985
Size: 508 2459 18
Fragments arranged by size:
2459 508 18
PspXI vC'TCGA_Gb
Cuts at: 0 137 2985
Size: 137 2848
PstI C_TGCA'G
Cuts at: 0 604 1118 1952 2006 2857 2985
Size: 604 514 834 54 851 128
Fragments arranged by size:
851 834 604 514 128 54
PvuII CAG'CTG
Cuts at: 0 1419 1482 2340 2985
Size: 1419 63 858 645
Fragments arranged by size:
1419 858 645 63
ScaI AGT'ACT
Cuts at: 0 2806 2985
Size: 2806 179
SfcI C'TryA_G
Cuts at: 0 600 1114 1948 2002 2853 2985
Size: 600 514 834 54 851 132
Fragments arranged by size:
851 834 600 514 132 54
SmlI C'TyrA_G
Cuts at: 0 137 2985
Size: 137 2848
StyI C'CwwG_G
Cuts at: 0 839 866 2408 2985
Size: 839 27 1542 577
Fragments arranged by size:
1542 839 577 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1302 2761 2985
Size: 1302 1459 224
Fragments arranged by size:
1459 1302 224
TatI w'GTAC_w
Cuts at: 0 292 731 2804 2985
Size: 292 439 2073 181
Fragments arranged by size:
2073 439 292 181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 18 50 685 717 1528 1560 2985
Size: 18 32 635 32 811 32 1425
Fragments arranged by size:
1425 811 635 32 32 32 18
Tth111I GACn'n_nGTC
Cuts at: 0 1027 1566 2032 2985
Size: 1027 539 466 953
Fragments arranged by size:
1027 953 539 466
XbaI T'CTAG_A
Cuts at: 0 2613 2985
Size: 2613 372
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 565 1974 2679 2770 2985
Size: 565 1409 705 91 215
Fragments arranged by size:
1409 705 565 215 91
XhoI C'TCGA_G
Cuts at: 0 137 2985
Size: 137 2848
XmnI GAAnn'nnTTC
Cuts at: 0 1411 1855 2985
Size: 1411 444 1130
Fragments arranged by size:
1411 1130 444
ZraI GAC'GTC
Cuts at: 0 2031 2985
Size: 2031 954
Enzymes that do cut:
AarI AatII AcuI AfeI AgeI AlwNI ApaI ApoI
AvaI BaeI BanI BanII BbsI BbvCI BcgI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI HaeII Hin4I HincII MmeI MslI
MspA1I NaeI NgoMIV NheI NsiI NspI PasI PflMI
PfoI PmlI PpuMI PspOMI PspXI PstI PvuII ScaI
SfcI SmlI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AhdI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BamHI BbeI
BciVI BpuEI BsaBI BsiWI BsmBI BspEI BsrBI BssHII
BstBI BstZ17I ClaI DraI EcoICRI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MscI NarI NcoI
NdeI NotI NruI PacI PciI PmeI PpiI PshAI
PsiI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI XmaI