Restriction map for
pFN21AE2561
(Linear) (Six-base) MAPSORT of: KIEE2561.seq Check: 7574 from: 1 to: 7653
KIEE2561.orf
With 182 enzymes: SgfI *
March 4, 2015 11:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2689 6901 7653
Size: 2689 4212 752
Fragments arranged by size:
4212 2689 752
AatII G_ACGT'C
Cuts at: 0 3396 3593 7653
Size: 3396 197 4060
Fragments arranged by size:
4060 3396 197
AccI GT'mk_AC
Cuts at: 0 874 2108 6563 7653
Size: 874 1234 4455 1090
Fragments arranged by size:
4455 1234 1090 874
Acc65I G'GTAC_C
Cuts at: 0 4979 7653
Size: 4979 2674
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 662 2152 3649 4116 5440 7631 7653
Size: 401 261 1490 1497 467 1324 2191 22
Fragments arranged by size:
2191 1497 1490 1324 467 401 261 22
AflIII A'CryG_T
Cuts at: 0 1078 1355 3242 6194 6196 7653
Size: 1078 277 1887 2952 2 1457
Fragments arranged by size:
2952 1887 1457 1078 277 2
AleI CACnn'nnGTG
Cuts at: 0 2029 2102 2683 4108 6244 6449 7653
Size: 2029 73 581 1425 2136 205 1204
Fragments arranged by size:
2136 2029 1425 1204 581 205 73
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 514 2778 5602 6560 7204 7653
Size: 514 2264 2824 958 644 449
Fragments arranged by size:
2824 2264 958 644 514 449
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5377 5409 7653
Size: 5377 32 2244
Fragments arranged by size:
5377 2244 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1878 3228 5849 6951 7653
Size: 1878 1350 2621 1102 702
Fragments arranged by size:
2621 1878 1350 1102 702
ApaI G_GGCC'C
Cuts at: 0 319 2495 7653
Size: 319 2176 5158
Fragments arranged by size:
5158 2176 319
ApaLI G'TGCA_C
Cuts at: 0 4491 4587 7653
Size: 4491 96 3066
Fragments arranged by size:
4491 3066 96
ApoI r'AATT_y
Cuts at: 0 48 618 936 1931 3797 4702 5181 5216
Size: 48 570 318 995 1866 905 479 35
Cuts at: 5216 5263 7653
Size: 47 2390
Fragments arranged by size:
2390 1866 995 905 570 479 318 48
47 35
AseI AT'TA_AT
Cuts at: 0 7502 7653
Size: 7502 151
AvaI C'yCGr_G
Cuts at: 0 2797 4931 5694 6435 7016 7653
Size: 2797 2134 763 741 581 637
Fragments arranged by size:
2797 2134 763 741 637 581
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 6572 6605 7653
Size: 6572 33 1048
Fragments arranged by size:
6572 1048 33
BanI G'GyrC_C
Cuts at: 0 2671 3965 4979 7063 7180 7653
Size: 2671 1294 1014 2084 117 473
Fragments arranged by size:
2671 2084 1294 1014 473 117
BanII G_rGCy'C
Cuts at: 0 319 2081 2495 5600 6079 7653
Size: 319 1762 414 3105 479 1574
Fragments arranged by size:
3105 1762 1574 479 414 319
BbsI GAAGACnn'nnnn_
Cuts at: 0 3224 3951 4304 5503 6852 7653
Size: 3224 727 353 1199 1349 801
Fragments arranged by size:
3224 1349 1199 801 727 353
BbvCI CC'TCA_GC
Cuts at: 0 2481 7569 7653
Size: 2481 5088 84
Fragments arranged by size:
5088 2481 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 716 750 1002 1022 1036 1056 5529 5563
Size: 716 34 252 20 14 20 4473 34
Cuts at: 5563 7653
Size: 2090
Fragments arranged by size:
4473 2090 716 252 34 34 20 20
14
BciVI GTATCCnnnnn_n'
Cuts at: 0 6331 6573 7653
Size: 6331 242 1080
Fragments arranged by size:
6331 1080 242
BclI T'GATC_A
Cuts at: 0 5931 7653
Size: 5931 1722
BfrBI ATG'CAT
Cuts at: 0 1043 2291 2654 3654 3920 7653
Size: 1043 1248 363 1000 266 3733
Fragments arranged by size:
3733 1248 1043 1000 363 266
BglI GCCn_nnn'nGGC
Cuts at: 0 314 797 2276 2432 6652 6712 7653
Size: 314 483 1479 156 4220 60 941
Fragments arranged by size:
4220 1479 941 483 314 156 60
BglII A'GATC_T
Cuts at: 0 1594 3216 4866 7653
Size: 1594 1622 1650 2787
Fragments arranged by size:
2787 1650 1622 1594
BlpI GC'TnA_GC
Cuts at: 0 6732 7011 7653
Size: 6732 279 642
Fragments arranged by size:
6732 642 279
Bme1580I G_kGCm'C
Cuts at: 0 163 319 337 2026 2148 2495 2674 4495
Size: 163 156 18 1689 122 347 179 1821
Cuts at: 4495 4591 6310 6356 7653
Size: 96 1719 46 1297
Fragments arranged by size:
1821 1719 1689 1297 347 179 163 156
122 96 46 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 2029 2313 2744 4733 4824 4953 5320 6625
Size: 2029 284 431 1989 91 129 367 1305
Cuts at: 6625 7424 7653
Size: 799 229
Fragments arranged by size:
2029 1989 1305 799 431 367 284 229
129 91
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 567 2057 7557 7653
Size: 567 1490 5500 96
Fragments arranged by size:
5500 1490 567 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 1712 2073 2609 2952 3634 4661 5909
Size: 968 744 361 536 343 682 1027 1248
Cuts at: 5909 6766 7653
Size: 857 887
Fragments arranged by size:
1248 1027 968 887 857 744 682 536
361 343
Bpu10I CC'TnA_GC
Cuts at: 0 2244 2481 3688 7569 7653
Size: 2244 237 1207 3881 84
Fragments arranged by size:
3881 2244 1207 237 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7645 7653
Size: 7645 8
BsaI GGTCTCn'nnnn_
Cuts at: 0 3655 4144 6321 6742 7099 7246 7653
Size: 3655 489 2177 421 357 147 407
Fragments arranged by size:
3655 2177 489 421 407 357 147
BsaAI yAC'GTr
Cuts at: 0 758 1062 4496 6197 7653
Size: 758 304 3434 1701 1456
Fragments arranged by size:
3434 1701 1456 758 304
BsaBI GATnn'nnATC
Cuts at: 0 1330 3361 5836 7653
Size: 1330 2031 2475 1817
Fragments arranged by size:
2475 2031 1817 1330
BsaHI Gr'CG_yC
Cuts at: 0 3393 3590 6551 7653
Size: 3393 197 2961 1102
Fragments arranged by size:
3393 2961 1102 197
BsaWI w'CCGG_w
Cuts at: 0 3596 5947 7457 7653
Size: 3596 2351 1510 196
Fragments arranged by size:
3596 2351 1510 196
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2046 2076 2451 2481 5587 5617 6682 6712
Size: 2046 30 375 30 3106 30 1065 30
Cuts at: 6712 7243 7273 7557 7587 7653
Size: 531 30 284 30 66
Fragments arranged by size:
3106 2046 1065 531 375 284 66 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2314 3598 3631 3852 3927 4283 7653
Size: 2314 1284 33 221 75 356 3370
Fragments arranged by size:
3370 2314 1284 356 221 75 33
BseYI C'CCAG_C
Cuts at: 0 60 311 472 1203 1784 3669 5725 7092
Size: 60 251 161 731 581 1885 2056 1367
Cuts at: 7092 7200 7653
Size: 108 453
Fragments arranged by size:
2056 1885 1367 731 581 453 251 161
108 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 978 1477 2282 2521 2774 3625 4140
Size: 617 361 499 805 239 253 851 515
Cuts at: 4140 4389 4394 4641 5608 5841 6345 6401 6873
Size: 249 5 247 967 233 504 56 472
Cuts at: 6873 7653
Size: 780
Fragments arranged by size:
967 851 805 780 617 515 504 499
472 361 253 249 247 239 233 56
5
BsiHKAI G_wGCw'C
Cuts at: 0 4495 4591 5086 5600 6413 6733 7133 7564
Size: 4495 96 495 514 813 320 400 431
Cuts at: 7564 7653
Size: 89
Fragments arranged by size:
4495 813 514 495 431 400 320 96
89
BsmI GAATG_Cn'
Cuts at: 0 667 1008 1045 2656 3495 4009 4056 4584
Size: 667 341 37 1611 839 514 47 528
Cuts at: 4584 7653
Size: 3069
Fragments arranged by size:
3069 1611 839 667 528 514 341 47
37
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2438 5079 7653
Size: 2438 2641 2574
Fragments arranged by size:
2641 2574 2438
Bsp1286I G_dGCh'C
Cuts at: 0 163 319 337 2026 2081 2148 2495 2674
Size: 163 156 18 1689 55 67 347 179
Cuts at: 2674 4495 4591 5086 5600 6079 6310 6356 6413
Size: 1821 96 495 514 479 231 46 57
Cuts at: 6413 6733 7133 7564 7653
Size: 320 400 431 89
Fragments arranged by size:
1821 1689 514 495 479 431 400 347
320 231 179 163 156 96 89 67
57 55 46 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 203 412 2108 2689 3425 3647 3883 4162
Size: 203 209 1696 581 736 222 236 279
Cuts at: 4162 4789 5379 5584 5789 6901 6936 6947 7653
Size: 627 590 205 205 1112 35 11 706
Fragments arranged by size:
1696 1112 736 706 627 590 581 279
236 222 209 205 205 203 35 11
BsrBI CCG'CTC
Cuts at: 0 346 2138 5817 7653
Size: 346 1792 3679 1836
Fragments arranged by size:
3679 1836 1792 346
BsrDI GCAATG_nn'
Cuts at: 0 366 1472 1901 2163 2544 2649 4314 4440
Size: 366 1106 429 262 381 105 1665 126
Cuts at: 4440 7638 7653
Size: 3198 15
Fragments arranged by size:
3198 1665 1106 429 381 366 262 126
105 15
BsrFI r'CCGG_y
Cuts at: 0 4012 5968 7653
Size: 4012 1956 1685
Fragments arranged by size:
4012 1956 1685
BsrGI T'GTAC_A
Cuts at: 0 42 784 7653
Size: 42 742 6869
Fragments arranged by size:
6869 742 42
BssSI C'ACGA_G
Cuts at: 0 3094 6117 6406 7653
Size: 3094 3023 289 1247
Fragments arranged by size:
3094 3023 1247 289
BstAPI GCAn_nnn'nTGC
Cuts at: 0 701 4145 4417 4529 7348 7653
Size: 701 3444 272 112 2819 305
Fragments arranged by size:
3444 2819 701 305 272 112
BstEII G'GTnAC_C
Cuts at: 0 350 5551 7653
Size: 350 5201 2102
Fragments arranged by size:
5201 2102 350
BstXI CCAn_nnnn'nTGG
Cuts at: 0 6793 7407 7653
Size: 6793 614 246
Fragments arranged by size:
6793 614 246
BstYI r'GATC_y
Cuts at: 0 1594 1735 3216 4866 5472 5837 7388 7653
Size: 1594 141 1481 1650 606 365 1551 265
Fragments arranged by size:
1650 1594 1551 1481 606 365 265 141
BtgI C'CryG_G
Cuts at: 0 804 1431 1511 3774 4206 4560 4785 6048
Size: 804 627 80 2263 432 354 225 1263
Cuts at: 6048 6677 7059 7653
Size: 629 382 594
Fragments arranged by size:
2263 1263 804 629 627 594 432 382
354 225 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 545 704 2429 6074 7653
Size: 545 159 1725 3645 1579
Fragments arranged by size:
3645 1725 1579 545 159
BtsI GCAGTG_nn'
Cuts at: 0 909 1913 2999 5486 6091 6152 6544 6936
Size: 909 1004 1086 2487 605 61 392 392
Cuts at: 6936 7220 7291 7653
Size: 284 71 362
Fragments arranged by size:
2487 1086 1004 909 605 392 392 362
284 71 61
ClaI AT'CG_AT
Cuts at: 0 1989 7653
Size: 1989 5664
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1871 1906 7653
Size: 1871 35 5747
Fragments arranged by size:
5747 1871 35
DraI TTT'AAA
Cuts at: 0 7337 7653
Size: 7337 316
DrdI GACnn_nn'nnGTC
Cuts at: 0 1377 7050 7653
Size: 1377 5673 603
Fragments arranged by size:
5673 1377 603
EaeI y'GGCC_r
Cuts at: 0 306 813 1189 1796 1832 1926 5568 5942
Size: 306 507 376 607 36 94 3642 374
Cuts at: 5942 6001 6287 7653
Size: 59 286 1366
Fragments arranged by size:
3642 1366 607 507 376 374 306 286
94 59 36
EarI CTCTTCn'nnn_
Cuts at: 0 110 2095 2128 3904 5341 7266 7487 7653
Size: 110 1985 33 1776 1437 1925 221 166
Fragments arranged by size:
1985 1925 1776 1437 221 166 110 33
EcoICRI GAG'CTC
Cuts at: 0 5598 7653
Size: 5598 2055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 662 968 1712 2073 2152 2609 2952
Size: 401 261 306 744 361 79 457 343
Cuts at: 2952 3634 3649 4116 4661 5440 5909 6766 7631
Size: 682 15 467 545 779 469 857 865
Cuts at: 7631 7653
Size: 22
Fragments arranged by size:
865 857 779 744 682 545 469 467
457 401 361 343 306 261 79 22
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2612 5790 6943 7653
Size: 2612 3178 1153 710
Fragments arranged by size:
3178 2612 1153 710
EcoO109I rG'GnC_Cy
Cuts at: 0 315 1207 2420 2492 2794 3314 4225 4600
Size: 315 892 1213 72 302 520 911 375
Cuts at: 4600 4882 6112 6948 7233 7653
Size: 282 1230 836 285 420
Fragments arranged by size:
1230 1213 911 892 836 520 420 375
315 302 285 282 72
EcoRI G'AATT_C
Cuts at: 0 5216 7653
Size: 5216 2437
EcoRV GAT'ATC
Cuts at: 0 5127 7653
Size: 5127 2526
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3588 5877 6651 7653
Size: 3588 2289 774 1002
Fragments arranged by size:
3588 2289 1002 774
HaeII r_GCGC'y
Cuts at: 0 4331 7653
Size: 4331 3322
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 85 115 117 147 2046 2078 2761 2793
Size: 85 30 2 30 1899 32 683 32
Cuts at: 2793 5923 5955 6907 6939 7096 7128 7373 7405
Size: 3130 32 952 32 157 32 245 32
Cuts at: 7405 7653
Size: 248
Fragments arranged by size:
3130 1899 952 683 248 245 157 85
32 32 32 32 32 32 30 30
2
HincII GTy'rAC
Cuts at: 0 6564 7653
Size: 6564 1089
HindIII A'AGCT_T
Cuts at: 0 1178 1420 1744 3141 7653
Size: 1178 242 324 1397 4512
Fragments arranged by size:
4512 1397 1178 324 242
KpnI G_GTAC'C
Cuts at: 0 4983 7653
Size: 4983 2670
MfeI C'AATT_G
Cuts at: 0 3305 3494 7653
Size: 3305 189 4159
Fragments arranged by size:
4159 3305 189
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 2680 6453 7028 7266 7469 7653
Size: 312 2368 3773 575 238 203 184
Fragments arranged by size:
3773 2368 575 312 238 203 184
MscI TGG'CCA
Cuts at: 0 815 1191 1798 1834 5570 6289 7653
Size: 815 376 607 36 3736 719 1364
Fragments arranged by size:
3736 1364 815 719 607 376 36
MslI CAynn'nnrTG
Cuts at: 0 486 743 1001 1354 1991 2029 2100 2102
Size: 486 257 258 353 637 38 71 2
Cuts at: 2102 2683 4108 4368 6244 6449 7408 7653
Size: 581 1425 260 1876 205 959 245
Fragments arranged by size:
1876 1425 959 637 581 486 353 260
258 257 245 205 71 38 2
MspA1I CmG'CkG
Cuts at: 0 64 311 476 4136 4787 5004 5592 5666
Size: 64 247 165 3660 651 217 588 74
Cuts at: 5666 5729 6326 6359 6504 6518 7061 7653
Size: 63 597 33 145 14 543 592
Fragments arranged by size:
3660 651 597 592 588 543 247 217
165 145 74 64 63 33 14
NaeI GCC'GGC
Cuts at: 0 4014 7653
Size: 4014 3639
NcoI C'CATG_G
Cuts at: 0 1431 1511 3774 4206 4560 6677 7653
Size: 1431 80 2263 432 354 2117 976
Fragments arranged by size:
2263 2117 1431 976 432 354 80
NdeI CA'TA_TG
Cuts at: 0 3188 7653
Size: 3188 4465
NgoMIV G'CCGG_C
Cuts at: 0 4012 7653
Size: 4012 3641
NsiI A_TGCA'T
Cuts at: 0 1045 2293 2656 3656 3922 7653
Size: 1045 1248 363 1000 266 3731
Fragments arranged by size:
3731 1248 1045 1000 363 266
NspI r_CATG'y
Cuts at: 0 791 1359 1627 2291 2396 2441 3246 3654
Size: 791 568 268 664 105 45 805 408
Cuts at: 3654 3860 4196 4319 6239 7155 7653
Size: 206 336 123 1920 916 498
Fragments arranged by size:
1920 916 805 791 664 568 498 408
336 268 206 123 105 45
PasI CC'CwG_GG
Cuts at: 0 5655 7653
Size: 5655 1998
PciI A'CATG_T
Cuts at: 0 1355 3242 7653
Size: 1355 1887 4411
Fragments arranged by size:
4411 1887 1355
PflMI CCAn_nnn'nTGG
Cuts at: 0 3780 5492 7653
Size: 3780 1712 2161
Fragments arranged by size:
3780 2161 1712
PfoI T'CCnGG_A
Cuts at: 0 2220 2625 3611 6575 7653
Size: 2220 405 986 2964 1078
Fragments arranged by size:
2964 2220 1078 986 405
PmlI CAC'GTG
Cuts at: 0 4496 6197 7653
Size: 4496 1701 1456
Fragments arranged by size:
4496 1701 1456
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 225 257 3879 3911 6781 6813 7653
Size: 225 32 3622 32 2870 32 840
Fragments arranged by size:
3622 2870 840 225 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1207 2794 4225 4600 6948 7233 7653
Size: 1207 1587 1431 375 2348 285 420
Fragments arranged by size:
2348 1587 1431 1207 420 375 285
PspOMI G'GGCC_C
Cuts at: 0 315 2491 7653
Size: 315 2176 5162
Fragments arranged by size:
5162 2176 315
PspXI vC'TCGA_Gb
Cuts at: 0 7016 7653
Size: 7016 637
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1573 1605 3978 4010 7653
Size: 1573 32 2373 32 3643
Fragments arranged by size:
3643 2373 1573 32 32
PstI C_TGCA'G
Cuts at: 0 3224 4914 5581 6147 6325 6947 7286 7653
Size: 3224 1690 667 566 178 622 339 367
Fragments arranged by size:
3224 1690 667 622 566 367 339 178
PvuII CAG'CTG
Cuts at: 0 64 476 5004 5729 6326 6359 6504 7653
Size: 64 412 4528 725 597 33 145 1149
Fragments arranged by size:
4528 1149 725 597 412 145 64 33
SacI G_AGCT'C
Cuts at: 0 5600 7653
Size: 5600 2053
SacII CC_GC'GG
Cuts at: 0 4788 7062 7653
Size: 4788 2274 591
Fragments arranged by size:
4788 2274 591
SalI G'TCGA_C
Cuts at: 0 6562 7653
Size: 6562 1091
SanDI GG'GwC_CC
Cuts at: 0 1207 2794 7653
Size: 1207 1587 4859
Fragments arranged by size:
4859 1587 1207
SapI GCTCTTCn'nnn_
Cuts at: 0 2128 7266 7653
Size: 2128 5138 387
Fragments arranged by size:
5138 2128 387
ScaI AGT'ACT
Cuts at: 0 5136 5938 7653
Size: 5136 802 1715
Fragments arranged by size:
5136 1715 802
SexAI A'CCwGG_T
Cuts at: 0 7255 7653
Size: 7255 398
SfcI C'TryA_G
Cuts at: 0 194 875 1229 2109 2634 3220 4695 4910
Size: 194 681 354 880 525 586 1475 215
Cuts at: 4910 5577 6143 6321 6943 7282 7653
Size: 667 566 178 622 339 371
Fragments arranged by size:
1475 880 681 667 622 586 566 525
371 354 339 215 194 178
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 314 7653
Size: 314 7339
SmaI CCC'GGG
Cuts at: 0 2799 6437 7653
Size: 2799 3638 1216
Fragments arranged by size:
3638 2799 1216
SmlI C'TyrA_G
Cuts at: 0 7016 7624 7641 7653
Size: 7016 608 17 12
Fragments arranged by size:
7016 608 17 12
SnaBI TAC'GTA
Cuts at: 0 758 7653
Size: 758 6895
SphI G_CATG'C
Cuts at: 0 3654 7653
Size: 3654 3999
SrfI GCCC'GGGC
Cuts at: 0 6437 7653
Size: 6437 1216
SspI AAT'ATT
Cuts at: 0 3165 7653
Size: 3165 4488
StuI AGG'CCT
Cuts at: 0 5881 6655 7332 7653
Size: 5881 774 677 321
Fragments arranged by size:
5881 774 677 321
StyI C'CwwG_G
Cuts at: 0 147 1431 1511 1576 2310 3774 4206 4221
Size: 147 1284 80 65 734 1464 432 15
Cuts at: 4221 4229 4386 4451 4560 5049 5456 5734 6677
Size: 8 157 65 109 489 407 278 943
Cuts at: 6677 7427 7653
Size: 750 226
Fragments arranged by size:
1464 1284 943 750 734 489 432 407
278 226 157 147 109 80 65 65
15 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 136 1054 2689 3953 5602 7653
Size: 136 918 1635 1264 1649 2051
Fragments arranged by size:
2051 1649 1635 1264 918 136
TatI w'GTAC_w
Cuts at: 0 42 784 2048 4744 5134 5936 7653
Size: 42 742 1264 2696 390 802 1717
Fragments arranged by size:
2696 1717 1264 802 742 390 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2451 2483 2835 2867 4472 4504 5973 6005
Size: 2451 32 352 32 1605 32 1469 32
Cuts at: 6005 7653
Size: 1648
Fragments arranged by size:
2451 1648 1605 1469 352 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 3228 7110 7171 7653
Size: 3228 3882 61 482
Fragments arranged by size:
3882 3228 482 61
XbaI T'CTAG_A
Cuts at: 0 3579 7032 7653
Size: 3579 3453 621
Fragments arranged by size:
3579 3453 621
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 421 850 1315 7653
Size: 421 429 465 6338
Fragments arranged by size:
6338 465 429 421
XhoI C'TCGA_G
Cuts at: 0 7016 7653
Size: 7016 637
XmaI C'CCGG_G
Cuts at: 0 2797 6435 7653
Size: 2797 3638 1218
Fragments arranged by size:
3638 2797 1218
XmnI GAAnn'nnTTC
Cuts at: 0 1249 3142 7653
Size: 1249 1893 4511
Fragments arranged by size:
4511 1893 1249
ZraI GAC'GTC
Cuts at: 0 3394 3591 7653
Size: 3394 197 4062
Fragments arranged by size:
4062 3394 197
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AvaI BaeI
BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII KpnI MfeI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI
PspXI PsrI PstI PvuII SacI SacII SalI SanDI
SapI ScaI SexAI SfcI SfiI SmaI SmlI SnaBI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AhdI AscI AsiSI AvrII
BamHI BbeI BmgBI BmtI BsiEI BsiWI BspEI BspHI
BssHII BstBI BstZ17I Bsu36I DraIII EagI EciI FseI
FspI FspAI HpaI KasI MluI NarI NheI NotI
NruI PacI PmeI PshAI PsiI PvuI RsrII SbfI
SfoI SgfI SgrAI SpeI SwaI