(Linear) (Six-base) MAPSORT of: KIEE2559.seq  Check: 6542  from: 1  to: 441



KIEE2559.orf

 With 182 enzymes: SgfI * 

                        January 9, 2015 15:32  ..

AfeI AGC'GCT

Cuts at:      0     127     441 
   Size:        127     314
 
AlwNI CAG_nnn'CTG

Cuts at:      0     189     428     441 
   Size:        189     239      13

  Fragments arranged by size: 

                 239     189      13
 
BbvCI CC'TCA_GC

Cuts at:      0     208     441 
   Size:        208     233
 
BglII A'GATC_T

Cuts at:      0     308     441 
   Size:        308     133
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     195     441 
   Size:        195     246
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     441 
   Size:        403      38
 
Bpu10I CC'TnA_GC

Cuts at:      0     208     441 
   Size:        208     233
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     441 
   Size:        436       5
 
BsaWI w'CCGG_w

Cuts at:      0     218     441 
   Size:        218     223
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     133     441 
   Size:        133     308
 
BspEI T'CCGG_A

Cuts at:      0     218     441 
   Size:        218     223
 
BsrGI T'GTAC_A

Cuts at:      0     344     441 
   Size:        344      97
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     174     441 
   Size:        174     267
 
BstYI r'GATC_y

Cuts at:      0     308     441 
   Size:        308     133
 
BtgI C'CryG_G

Cuts at:      0     242     299     441 
   Size:        242      57     142

  Fragments arranged by size: 

                 242     142      57
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     441 
   Size:        403      38
 
HaeII r_GCGC'y

Cuts at:      0     129     441 
   Size:        129     312
 
HindIII A'AGCT_T

Cuts at:      0      51     441 
   Size:         51     390
 
MspA1I CmG'CkG

Cuts at:      0     156     441 
   Size:        156     285
 
NcoI C'CATG_G

Cuts at:      0     299     441 
   Size:        299     142
 
PasI CC'CwG_GG

Cuts at:      0     134     398     441 
   Size:        134     264      43

  Fragments arranged by size: 

                 264     134      43
 
SfcI C'TryA_G

Cuts at:      0     312     441 
   Size:        312     129
 
SmlI C'TyrA_G

Cuts at:      0     415     441 
   Size:        415      26
 
StyI C'CwwG_G

Cuts at:      0     299     441 
   Size:        299     142
 
TatI w'GTAC_w

Cuts at:      0     344     441 
   Size:        344      97
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0       2      34     441 
   Size:          2      32     407

  Fragments arranged by size: 

                 407      32       2
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     370     441 
   Size:        370      71
 

 Enzymes that do cut:

     AfeI    AlwNI    BbvCI    BglII     BmrI     BpmI   Bpu10I    BpuEI
    BsaWI    BsmBI    BspEI    BsrGI    BstXI    BstYI     BtgI  Eco57MI
    HaeII  HindIII   MspA1I     NcoI     PasI     SfcI     SmlI     StyI
     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmtI     BplI     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI