Restriction map for
pFN21AE2558
(Linear) (Six-base) MAPSORT of: KIEE2558.seq Check: 188 from: 1 to: 2370
KIEE2558.orf
With 182 enzymes: SgfI *
December 25, 2014 17:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 191 2370
Size: 191 2179
AccI GT'mk_AC
Cuts at: 0 2249 2351 2370
Size: 2249 102 19
Fragments arranged by size:
2249 102 19
Acc65I G'GTAC_C
Cuts at: 0 2165 2370
Size: 2165 205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1455 1639 2370
Size: 607 848 184 731
Fragments arranged by size:
848 731 607 184
AfeI AGC'GCT
Cuts at: 0 481 2370
Size: 481 1889
AflIII A'CryG_T
Cuts at: 0 2108 2370
Size: 2108 262
AhdI GACnn_n'nnGTC
Cuts at: 0 1969 2370
Size: 1969 401
AleI CACnn'nnGTG
Cuts at: 0 292 877 2370
Size: 292 585 1493
Fragments arranged by size:
1493 585 292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2062 2211 2370
Size: 2062 149 159
Fragments arranged by size:
2062 159 149
AlwNI CAG_nnn'CTG
Cuts at: 0 234 243 426 522 1863 1965 2370
Size: 234 9 183 96 1341 102 405
Fragments arranged by size:
1341 405 234 183 102 96 9
ApaI G_GGCC'C
Cuts at: 0 165 545 2370
Size: 165 380 1825
Fragments arranged by size:
1825 380 165
ApaLI G'TGCA_C
Cuts at: 0 1921 2311 2370
Size: 1921 390 59
Fragments arranged by size:
1921 390 59
AvaI C'yCGr_G
Cuts at: 0 135 152 1159 2068 2370
Size: 135 17 1007 909 302
Fragments arranged by size:
1007 909 302 135 17
BamHI G'GATC_C
Cuts at: 0 680 2370
Size: 680 1690
BanI G'GyrC_C
Cuts at: 0 94 318 597 641 927 1537 2165 2340
Size: 94 224 279 44 286 610 628 175
Cuts at: 2340 2370
Size: 30
Fragments arranged by size:
628 610 286 279 224 175 94 44
30
BanII G_rGCy'C
Cuts at: 0 165 212 251 545 1439 2285 2370
Size: 165 47 39 294 894 846 85
Fragments arranged by size:
894 846 294 165 85 47 39
BbeI G_GCGC'C
Cuts at: 0 98 322 1541 2370
Size: 98 224 1219 829
Fragments arranged by size:
1219 829 224 98
BbsI GAAGACnn'nnnn_
Cuts at: 0 1545 2370
Size: 1545 825
BglI GCCn_nnn'nGGC
Cuts at: 0 128 2370
Size: 128 2242
BglII A'GATC_T
Cuts at: 0 2138 2370
Size: 2138 232
BlpI GC'TnA_GC
Cuts at: 0 1664 2370
Size: 1664 706
Bme1580I G_kGCm'C
Cuts at: 0 165 465 545 581 602 1925 2315 2370
Size: 165 300 80 36 21 1323 390 55
Fragments arranged by size:
1323 390 300 165 80 55 36 21
BmgBI CAC'GTC
Cuts at: 0 630 791 1059 2109 2212 2370
Size: 630 161 268 1050 103 158
Fragments arranged by size:
1050 630 268 161 158 103
BmrI ACTGGGnnnn_n'
Cuts at: 0 508 2370
Size: 508 1862
BmtI G_CTAG'C
Cuts at: 0 1877 2370
Size: 1877 493
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 405 2370
Size: 405 1965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 400 685 715 1018 1214 1337 1820
Size: 265 135 285 30 303 196 123 483
Cuts at: 1820 2370
Size: 550
Fragments arranged by size:
550 483 303 285 265 196 135 123
30
Bpu10I CC'TnA_GC
Cuts at: 0 1583 2370
Size: 1583 787
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1501 2370
Size: 1501 869
BsaI GGTCTCn'nnnn_
Cuts at: 0 310 831 964 1009 1190 1239 2370
Size: 310 521 133 45 181 49 1131
Fragments arranged by size:
1131 521 310 181 133 49 45
BsaAI yAC'GTr
Cuts at: 0 2230 2370
Size: 2230 140
BsaHI Gr'CG_yC
Cuts at: 0 95 319 1538 1627 1712 2370
Size: 95 224 1219 89 85 658
Fragments arranged by size:
1219 658 224 95 89 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1187 1217 2158 2188 2370
Size: 1187 30 941 30 182
Fragments arranged by size:
1187 941 182 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 123 332 502 505 2370
Size: 123 209 170 3 1865
Fragments arranged by size:
1865 209 170 123 3
BseYI C'CCAG_C
Cuts at: 0 41 482 794 1527 2285 2370
Size: 41 441 312 733 758 85
Fragments arranged by size:
758 733 441 312 85 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 433 853 883 1701 2370
Size: 424 9 420 30 818 669
Fragments arranged by size:
818 669 424 420 30 9
BsiHKAI G_wGCw'C
Cuts at: 0 212 251 965 1298 1517 1641 1925 2132
Size: 212 39 714 333 219 124 284 207
Cuts at: 2132 2315 2370
Size: 183 55
Fragments arranged by size:
714 333 284 219 212 207 183 124
55 39
BsmBI CGTCTCn'nnnn_
Cuts at: 0 849 938 2370
Size: 849 89 1432
Fragments arranged by size:
1432 849 89
Bsp1286I G_dGCh'C
Cuts at: 0 165 212 251 465 545 581 602 965
Size: 165 47 39 214 80 36 21 363
Cuts at: 965 1298 1439 1517 1641 1925 2132 2285 2315
Size: 333 141 78 124 284 207 153 30
Cuts at: 2315 2370
Size: 55
Fragments arranged by size:
363 333 284 214 207 165 153 141
124 80 78 55 47 39 36 30
21
BspHI T'CATG_A
Cuts at: 0 1385 2370
Size: 1385 985
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 191 2370
Size: 191 2179
BsrBI CCG'CTC
Cuts at: 0 2223 2370
Size: 2223 147
BsrFI r'CCGG_y
Cuts at: 0 673 924 1002 2370
Size: 673 251 78 1368
Fragments arranged by size:
1368 673 251 78
BstAPI GCAn_nnn'nTGC
Cuts at: 0 234 1698 1920 2370
Size: 234 1464 222 450
Fragments arranged by size:
1464 450 234 222
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1812 2370
Size: 1812 558
BstYI r'GATC_y
Cuts at: 0 680 2138 2370
Size: 680 1458 232
Fragments arranged by size:
1458 680 232
BtgI C'CryG_G
Cuts at: 0 126 707 1791 2370
Size: 126 581 1084 579
Fragments arranged by size:
1084 581 579 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 159 2370
Size: 159 2211
BtsI GCAGTG_nn'
Cuts at: 0 532 2370
Size: 532 1838
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1287 1322 2370
Size: 1287 35 1048
Fragments arranged by size:
1287 1048 35
DraIII CAC_nnn'GTG
Cuts at: 0 1698 2370
Size: 1698 672
EaeI y'GGCC_r
Cuts at: 0 752 854 1101 1253 1367 1934 2224 2370
Size: 752 102 247 152 114 567 290 146
Fragments arranged by size:
752 567 290 247 152 146 114 102
EarI CTCTTCn'nnn_
Cuts at: 0 292 952 1276 2370
Size: 292 660 324 1094
Fragments arranged by size:
1094 660 324 292
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 26 518 1101 2370
Size: 26 492 583 1269
Fragments arranged by size:
1269 583 492 26
EcoICRI GAG'CTC
Cuts at: 0 210 249 2370
Size: 210 39 2121
Fragments arranged by size:
2121 210 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 400 607 685 715 1018 1214 1337
Size: 265 135 207 78 30 303 196 123
Cuts at: 1337 1455 1639 1820 2370
Size: 118 184 181 550
Fragments arranged by size:
550 303 265 207 196 184 181 135
123 118 78 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 667 2335 2370
Size: 667 1668 35
Fragments arranged by size:
1668 667 35
EcoO109I rG'GnC_Cy
Cuts at: 0 161 162 264 408 541 542 660 1164
Size: 161 1 102 144 133 1 118 504
Cuts at: 1164 1767 1840 1929 2370
Size: 603 73 89 441
Fragments arranged by size:
603 504 441 161 144 133 118 102
89 73 1 1
EcoRV GAT'ATC
Cuts at: 0 2004 2370
Size: 2004 366
HaeII r_GCGC'y
Cuts at: 0 98 322 483 1503 1541 2370
Size: 98 224 161 1020 38 829
Fragments arranged by size:
1020 829 224 161 98 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1955 1987 2370
Size: 1955 32 383
Fragments arranged by size:
1955 383 32
HincII GTy'rAC
Cuts at: 0 840 1062 2370
Size: 840 222 1308
Fragments arranged by size:
1308 840 222
KasI G'GCGC_C
Cuts at: 0 94 318 1537 2370
Size: 94 224 1219 833
Fragments arranged by size:
1219 833 224 94
KpnI G_GTAC'C
Cuts at: 0 2169 2370
Size: 2169 201
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2198 2201 2370
Size: 2198 3 169
Fragments arranged by size:
2198 169 3
MscI TGG'CCA
Cuts at: 0 754 1369 1936 2370
Size: 754 615 567 434
Fragments arranged by size:
754 615 567 434
MslI CAynn'nnrTG
Cuts at: 0 292 712 877 2370
Size: 292 420 165 1493
Fragments arranged by size:
1493 420 292 165
MspA1I CmG'CkG
Cuts at: 0 41 569 867 1860 2277 2370
Size: 41 528 298 993 417 93
Fragments arranged by size:
993 528 417 298 93 41
NaeI GCC'GGC
Cuts at: 0 675 926 1004 2370
Size: 675 251 78 1366
Fragments arranged by size:
1366 675 251 78
NarI GG'CG_CC
Cuts at: 0 95 319 1538 2370
Size: 95 224 1219 832
Fragments arranged by size:
1219 832 224 95
NcoI C'CATG_G
Cuts at: 0 1791 2370
Size: 1791 579
NgoMIV G'CCGG_C
Cuts at: 0 673 924 1002 2370
Size: 673 251 78 1368
Fragments arranged by size:
1368 673 251 78
NheI G'CTAG_C
Cuts at: 0 1873 2370
Size: 1873 497
NspI r_CATG'y
Cuts at: 0 558 2370
Size: 558 1812
PasI CC'CwG_GG
Cuts at: 0 738 1845 2370
Size: 738 1107 525
Fragments arranged by size:
1107 738 525
PflMI CCAn_nnn'nTGG
Cuts at: 0 1360 1965 2057 2370
Size: 1360 605 92 313
Fragments arranged by size:
1360 605 313 92
PfoI T'CCnGG_A
Cuts at: 0 391 995 2370
Size: 391 604 1375
Fragments arranged by size:
1375 604 391
PmlI CAC'GTG
Cuts at: 0 2230 2370
Size: 2230 140
PpuMI rG'GwC_Cy
Cuts at: 0 1164 1767 1929 2370
Size: 1164 603 162 441
Fragments arranged by size:
1164 603 441 162
PspOMI G'GGCC_C
Cuts at: 0 161 541 2370
Size: 161 380 1829
Fragments arranged by size:
1829 380 161
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1450 1482 2074 2106 2370
Size: 1450 32 592 32 264
Fragments arranged by size:
1450 592 264 32 32
PstI C_TGCA'G
Cuts at: 0 239 527 1685 2370
Size: 239 288 1158 685
Fragments arranged by size:
1158 685 288 239
PvuII CAG'CTG
Cuts at: 0 41 569 867 1860 2370
Size: 41 528 298 993 510
Fragments arranged by size:
993 528 510 298 41
SacI G_AGCT'C
Cuts at: 0 212 251 2370
Size: 212 39 2119
Fragments arranged by size:
2119 212 39
SapI GCTCTTCn'nnn_
Cuts at: 0 952 2370
Size: 952 1418
SfcI C'TryA_G
Cuts at: 0 235 475 523 1681 2007 2370
Size: 235 240 48 1158 326 363
Fragments arranged by size:
1158 363 326 240 235 48
SfoI GGC'GCC
Cuts at: 0 96 320 1539 2370
Size: 96 224 1219 831
Fragments arranged by size:
1219 831 224 96
SmaI CCC'GGG
Cuts at: 0 154 2370
Size: 154 2216
SmlI C'TyrA_G
Cuts at: 0 1516 2370
Size: 1516 854
StuI AGG'CCT
Cuts at: 0 1108 2370
Size: 1108 1262
StyI C'CwwG_G
Cuts at: 0 1440 1791 2370
Size: 1440 351 579
Fragments arranged by size:
1440 579 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1069 1384 2370
Size: 1069 315 986
Fragments arranged by size:
1069 986 315
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1187 1219 2370
Size: 1187 32 1151
Fragments arranged by size:
1187 1151 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 609 2370
Size: 609 1761
XmaI C'CCGG_G
Cuts at: 0 152 2370
Size: 152 2218
XmnI GAAnn'nnTTC
Cuts at: 0 1552 2370
Size: 1552 818
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII
BbeI BbsI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrFI BstAPI BstXI BstYI BtgI BtgZI
BtsI CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PasI PflMI PfoI PmlI PpuMI PspOMI
PsrI PstI PvuII SacI SapI SfcI SfoI SmaI
SmlI StuI StyI TaqII TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AloI ApoI AscI AseI
AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI
BsaBI BsaWI BsiEI BsiWI BsmI BspEI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI DraI
DrdI EagI EcoRI FalI FseI FspI FspAI HindIII
HpaI MfeI MluI NdeI NotI NruI NsiI PacI
PciI PmeI PpiI PshAI PsiI PspXI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI
Tth111I XbaI XhoI ZraI