Restriction map for
pFN21AE2547
(Linear) (Six-base) MAPSORT of: KIEE2547.seq Check: 601 from: 1 to: 1392
KIEE2547.orf
With 182 enzymes: SgfI *
October 24, 2014 10:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 322 1392
Size: 130 192 1070
Fragments arranged by size:
1070 192 130
ApaLI G'TGCA_C
Cuts at: 0 662 1392
Size: 662 730
ApoI r'AATT_y
Cuts at: 0 52 296 1392
Size: 52 244 1096
Fragments arranged by size:
1096 244 52
AvaI C'yCGr_G
Cuts at: 0 250 1392
Size: 250 1142
BamHI G'GATC_C
Cuts at: 0 1285 1392
Size: 1285 107
BanII G_rGCy'C
Cuts at: 0 853 912 1060 1392
Size: 853 59 148 332
Fragments arranged by size:
853 332 148 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 364 398 1392
Size: 364 34 994
Fragments arranged by size:
994 364 34
BglII A'GATC_T
Cuts at: 0 103 1392
Size: 103 1289
Bme1580I G_kGCm'C
Cuts at: 0 666 1392
Size: 666 726
BmgBI CAC'GTC
Cuts at: 0 661 1392
Size: 661 731
BmrI ACTGGGnnnn_n'
Cuts at: 0 742 1392
Size: 742 650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 1392
Size: 1007 385
BsaI GGTCTCn'nnnn_
Cuts at: 0 947 975 983 1392
Size: 947 28 8 409
Fragments arranged by size:
947 409 28 8
BsaHI Gr'CG_yC
Cuts at: 0 744 810 1392
Size: 744 66 582
Fragments arranged by size:
744 582 66
BsaWI w'CCGG_w
Cuts at: 0 1099 1392
Size: 1099 293
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 866 1392
Size: 866 526
BseYI C'CCAG_C
Cuts at: 0 160 1392
Size: 160 1232
BsiEI CG_ry'CG
Cuts at: 0 894 1392
Size: 894 498
BsiHKAI G_wGCw'C
Cuts at: 0 666 912 1392
Size: 666 246 480
Fragments arranged by size:
666 480 246
BsmI GAATG_Cn'
Cuts at: 0 137 162 397 553 794 1341 1392
Size: 137 25 235 156 241 547 51
Fragments arranged by size:
547 241 235 156 137 51 25
Bsp1286I G_dGCh'C
Cuts at: 0 666 853 912 1060 1392
Size: 666 187 59 148 332
Fragments arranged by size:
666 332 187 148 59
BspEI T'CCGG_A
Cuts at: 0 1099 1392
Size: 1099 293
BspHI T'CATG_A
Cuts at: 0 1242 1392
Size: 1242 150
BsrBI CCG'CTC
Cuts at: 0 372 1392
Size: 372 1020
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1067 1191 1392
Size: 1067 124 201
Fragments arranged by size:
1067 201 124
BstYI r'GATC_y
Cuts at: 0 103 1198 1285 1392
Size: 103 1095 87 107
Fragments arranged by size:
1095 107 103 87
BtsI GCAGTG_nn'
Cuts at: 0 173 249 350 1392
Size: 173 76 101 1042
Fragments arranged by size:
1042 173 101 76
DraI TTT'AAA
Cuts at: 0 99 1392
Size: 99 1293
DraIII CAC_nnn'GTG
Cuts at: 0 1093 1392
Size: 1093 299
EaeI y'GGCC_r
Cuts at: 0 891 1392
Size: 891 501
EagI C'GGCC_G
Cuts at: 0 891 1392
Size: 891 501
EarI CTCTTCn'nnn_
Cuts at: 0 77 320 765 825 929 969 1392
Size: 77 243 445 60 104 40 423
Fragments arranged by size:
445 423 243 104 77 60 40
EcoICRI GAG'CTC
Cuts at: 0 910 1392
Size: 910 482
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 322 1007 1392
Size: 130 192 685 385
Fragments arranged by size:
685 385 192 130
EcoNI CCTnn'n_nnAGG
Cuts at: 0 857 1280 1392
Size: 857 423 112
Fragments arranged by size:
857 423 112
EcoO109I rG'GnC_Cy
Cuts at: 0 330 1392
Size: 330 1062
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 622 654 840 872 1244 1276 1392
Size: 622 32 186 32 372 32 116
Fragments arranged by size:
622 372 186 116 32 32 32
HindIII A'AGCT_T
Cuts at: 0 535 1392
Size: 535 857
MfeI C'AATT_G
Cuts at: 0 287 1392
Size: 287 1105
MslI CAynn'nnrTG
Cuts at: 0 1065 1392
Size: 1065 327
MspA1I CmG'CkG
Cuts at: 0 1224 1392
Size: 1224 168
NdeI CA'TA_TG
Cuts at: 0 1137 1392
Size: 1137 255
PflMI CCAn_nnn'nTGG
Cuts at: 0 1093 1392
Size: 1093 299
PfoI T'CCnGG_A
Cuts at: 0 542 879 1392
Size: 542 337 513
Fragments arranged by size:
542 513 337
PstI C_TGCA'G
Cuts at: 0 72 338 1355 1392
Size: 72 266 1017 37
Fragments arranged by size:
1017 266 72 37
SacI G_AGCT'C
Cuts at: 0 912 1392
Size: 912 480
SfcI C'TryA_G
Cuts at: 0 68 334 1351 1392
Size: 68 266 1017 41
Fragments arranged by size:
1017 266 68 41
SspI AAT'ATT
Cuts at: 0 192 1392
Size: 192 1200
StyI C'CwwG_G
Cuts at: 0 320 1392
Size: 320 1072
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 363 1392
Size: 363 1029
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 734 766 1392
Size: 734 32 626
Fragments arranged by size:
734 626 32
XbaI T'CTAG_A
Cuts at: 0 106 826 1392
Size: 106 720 566
Fragments arranged by size:
720 566 106
Enzymes that do cut:
AcuI ApaLI ApoI AvaI BamHI BanII BcgI BglII
Bme1580I BmgBI BmrI BpmI BsaI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrBI
BstXI BstYI BtsI DraI DraIII EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I Hin4I HindIII MfeI MslI
MspA1I NdeI PflMI PfoI PstI SacI SfcI SspI
StyI TaqII TstI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI
AseI AsiSI AvrII BaeI BanI BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BlpI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaXI BsgI BsiWI BsmBI BspMI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DrdI EciI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MluI MmeI MscI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
SwaI TatI Tth111I XcmI XhoI XmaI XmnI ZraI