Restriction map for
pFN21AE2545
(Linear) (Six-base) MAPSORT of: KIEE2545.seq Check: 9559 from: 1 to: 606
KIEE2545.orf
With 182 enzymes: SgfI *
September 30, 2014 10:50 ..
AatII G_ACGT'C
Cuts at: 0 299 606
Size: 299 307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 606
Size: 574 32
AfeI AGC'GCT
Cuts at: 0 559 606
Size: 559 47
AflII C'TTAA_G
Cuts at: 0 307 606
Size: 307 299
AflIII A'CryG_T
Cuts at: 0 119 606
Size: 119 487
ApoI r'AATT_y
Cuts at: 0 256 606
Size: 256 350
AvaI C'yCGr_G
Cuts at: 0 200 606
Size: 200 406
BanI G'GyrC_C
Cuts at: 0 541 606
Size: 541 65
BanII G_rGCy'C
Cuts at: 0 367 440 606
Size: 367 73 166
Fragments arranged by size:
367 166 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 539 606
Size: 539 67
BbvCI CC'TCA_GC
Cuts at: 0 231 606
Size: 231 375
Bme1580I G_kGCm'C
Cuts at: 0 546 606
Size: 546 60
BmgBI CAC'GTC
Cuts at: 0 122 606
Size: 122 484
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 456 552 606
Size: 456 96 54
Fragments arranged by size:
456 96 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 606
Size: 451 155
Bpu10I CC'TnA_GC
Cuts at: 0 231 361 606
Size: 231 130 245
Fragments arranged by size:
245 231 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 606
Size: 315 291
BsaI GGTCTCn'nnnn_
Cuts at: 0 19 606
Size: 19 587
BsaHI Gr'CG_yC
Cuts at: 0 296 595 606
Size: 296 299 11
Fragments arranged by size:
299 296 11
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 85 115 606
Size: 85 30 491
Fragments arranged by size:
491 85 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 441 448 606
Size: 441 7 158
Fragments arranged by size:
441 158 7
BseYI C'CCAG_C
Cuts at: 0 337 344 366 401 414 606
Size: 337 7 22 35 13 192
Fragments arranged by size:
337 192 35 22 13 7
BsiEI CG_ry'CG
Cuts at: 0 13 606
Size: 13 593
Bsp1286I G_dGCh'C
Cuts at: 0 367 440 546 606
Size: 367 73 106 60
Fragments arranged by size:
367 106 73 60
BsrFI r'CCGG_y
Cuts at: 0 8 606
Size: 8 598
BstYI r'GATC_y
Cuts at: 0 425 606
Size: 425 181
Bsu36I CC'TnA_GG
Cuts at: 0 127 606
Size: 127 479
EaeI y'GGCC_r
Cuts at: 0 68 606
Size: 68 538
EarI CTCTTCn'nnn_
Cuts at: 0 521 606
Size: 521 85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 37 606
Size: 37 569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 574 606
Size: 451 123 32
Fragments arranged by size:
451 123 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 484 606
Size: 484 122
EcoRI G'AATT_C
Cuts at: 0 256 606
Size: 256 350
HaeII r_GCGC'y
Cuts at: 0 561 606
Size: 561 45
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 606
Size: 404 202
PfoI T'CCnGG_A
Cuts at: 0 260 428 606
Size: 260 168 178
Fragments arranged by size:
260 178 168
PshAI GACnn'nnGTC
Cuts at: 0 514 606
Size: 514 92
RsrII CG'GwC_CG
Cuts at: 0 15 606
Size: 15 591
SmlI C'TyrA_G
Cuts at: 0 307 330 606
Size: 307 23 276
Fragments arranged by size:
307 276 23
StyI C'CwwG_G
Cuts at: 0 41 485 606
Size: 41 444 121
Fragments arranged by size:
444 121 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3 93 103 606
Size: 3 90 10 503
Fragments arranged by size:
503 90 10 3
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 85 117 606
Size: 85 32 489
Fragments arranged by size:
489 85 32
Tth111I GACn'n_nGTC
Cuts at: 0 594 606
Size: 594 12
XmnI GAAnn'nnTTC
Cuts at: 0 497 559 606
Size: 497 62 47
Fragments arranged by size:
497 62 47
ZraI GAC'GTC
Cuts at: 0 297 606
Size: 297 309
Enzymes that do cut:
AatII AcuI AfeI AflII AflIII ApoI AvaI BanI
BanII BbsI BbvCI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiEI Bsp1286I
BsrFI BstYI Bsu36I EaeI EarI EciI Eco57MI EcoNI
EcoRI HaeII MmeI PfoI PshAI RsrII SmlI StyI
TaqII TstI Tth111I XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AgeI AhdI AleI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmrI BmtI BsaAI BsaBI BsaWI BsgI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoO109I EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI XbaI XcmI XhoI XmaI