Restriction map for
pFN21AE2544
(Linear) (Six-base) MAPSORT of: KIEE2544.seq Check: 9220 from: 1 to: 3231
KIEE2544.orf
With 182 enzymes: SgfI *
September 30, 2014 10:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 149 958 3036 3231
Size: 149 809 2078 195
Fragments arranged by size:
2078 809 195 149
AccI GT'mk_AC
Cuts at: 0 367 3231
Size: 367 2864
Acc65I G'GTAC_C
Cuts at: 0 272 3231
Size: 272 2959
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 797 985 1106 1499 1526 3231
Size: 605 192 188 121 393 27 1705
Fragments arranged by size:
1705 605 393 192 188 121 27
AleI CACnn'nnGTG
Cuts at: 0 1171 3231
Size: 1171 2060
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1513 1545 3231
Size: 1513 32 1686
Fragments arranged by size:
1686 1513 32
AlwNI CAG_nnn'CTG
Cuts at: 0 841 973 2080 3231
Size: 841 132 1107 1151
Fragments arranged by size:
1151 1107 841 132
ApoI r'AATT_y
Cuts at: 0 625 897 1018 1427 3231
Size: 625 272 121 409 1804
Fragments arranged by size:
1804 625 409 272 121
AvrII C'CTAG_G
Cuts at: 0 4 3231
Size: 4 3227
BamHI G'GATC_C
Cuts at: 0 302 1158 2586 3231
Size: 302 856 1428 645
Fragments arranged by size:
1428 856 645 302
BanI G'GyrC_C
Cuts at: 0 272 904 3231
Size: 272 632 2327
Fragments arranged by size:
2327 632 272
BanII G_rGCy'C
Cuts at: 0 1293 3231
Size: 1293 1938
BbsI GAAGACnn'nnnn_
Cuts at: 0 1289 1757 2135 3180 3231
Size: 1289 468 378 1045 51
Fragments arranged by size:
1289 1045 468 378 51
BbvCI CC'TCA_GC
Cuts at: 0 1436 3231
Size: 1436 1795
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1546 1580 3231
Size: 1546 34 1651
Fragments arranged by size:
1651 1546 34
BclI T'GATC_A
Cuts at: 0 2561 3231
Size: 2561 670
BglII A'GATC_T
Cuts at: 0 2658 3231
Size: 2658 573
BmrI ACTGGGnnnn_n'
Cuts at: 0 1263 1779 1833 3231
Size: 1263 516 54 1398
Fragments arranged by size:
1398 1263 516 54
Bpu10I CC'TnA_GC
Cuts at: 0 1183 1436 3152 3231
Size: 1183 253 1716 79
Fragments arranged by size:
1716 1183 253 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1373 1925 3231
Size: 1373 552 1306
Fragments arranged by size:
1373 1306 552
BsaI GGTCTCn'nnnn_
Cuts at: 0 403 3231
Size: 403 2828
BsaBI GATnn'nnATC
Cuts at: 0 2230 3231
Size: 2230 1001
BsaWI w'CCGG_w
Cuts at: 0 2222 3231
Size: 2222 1009
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2609 2639 3231
Size: 2609 30 592
Fragments arranged by size:
2609 592 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1928 3092 3231
Size: 1928 1164 139
Fragments arranged by size:
1928 1164 139
BseYI C'CCAG_C
Cuts at: 0 788 1851 2144 3231
Size: 788 1063 293 1087
Fragments arranged by size:
1087 1063 788 293
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1329 1872 3099 3231
Size: 1329 543 1227 132
Fragments arranged by size:
1329 1227 543 132
BsiHKAI G_wGCw'C
Cuts at: 0 1293 1405 3231
Size: 1293 112 1826
Fragments arranged by size:
1826 1293 112
BsmI GAATG_Cn'
Cuts at: 0 1187 3231
Size: 1187 2044
BsmBI CGTCTCn'nnnn_
Cuts at: 0 269 3231
Size: 269 2962
Bsp1286I G_dGCh'C
Cuts at: 0 1293 1405 3231
Size: 1293 112 1826
Fragments arranged by size:
1826 1293 112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 149 958 3036 3110 3231
Size: 149 809 2078 74 121
Fragments arranged by size:
2078 809 149 121 74
BsrDI GCAATG_nn'
Cuts at: 0 1882 3231
Size: 1882 1349
BsrGI T'GTAC_A
Cuts at: 0 1821 3231
Size: 1821 1410
BssSI C'ACGA_G
Cuts at: 0 1810 3231
Size: 1810 1421
BstBI TT'CG_AA
Cuts at: 0 1541 3231
Size: 1541 1690
BstXI CCAn_nnnn'nTGG
Cuts at: 0 127 208 2156 3231
Size: 127 81 1948 1075
Fragments arranged by size:
1948 1075 127 81
BstYI r'GATC_y
Cuts at: 0 302 1158 2586 2658 3231
Size: 302 856 1428 72 573
Fragments arranged by size:
1428 856 573 302 72
BtgI C'CryG_G
Cuts at: 0 908 2581 3231
Size: 908 1673 650
Fragments arranged by size:
1673 908 650
BtsI GCAGTG_nn'
Cuts at: 0 1755 1953 3231
Size: 1755 198 1278
Fragments arranged by size:
1755 1278 198
ClaI AT'CG_AT
Cuts at: 0 1694 3231
Size: 1694 1537
EaeI y'GGCC_r
Cuts at: 0 1200 1302 2082 3231
Size: 1200 102 780 1149
Fragments arranged by size:
1200 1149 780 102
EarI CTCTTCn'nnn_
Cuts at: 0 581 863 1502 1766 2155 2441 2813 3231
Size: 581 282 639 264 389 286 372 418
Fragments arranged by size:
639 581 418 389 372 286 282 264
EcoICRI GAG'CTC
Cuts at: 0 1291 3231
Size: 1291 1940
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 797 985 1106 1499 1526 3231
Size: 605 192 188 121 393 27 1705
Fragments arranged by size:
1705 605 393 192 188 121 27
EcoO109I rG'GnC_Cy
Cuts at: 0 1257 3231
Size: 1257 1974
EcoRV GAT'ATC
Cuts at: 0 1689 3231
Size: 1689 1542
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2152 3231
Size: 2152 1079
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 594 626 817 849 1552 1584 1676 1708
Size: 594 32 191 32 703 32 92 32
Cuts at: 1708 1765 1797 2609 2641 2943 2975 3191 3223
Size: 57 32 812 32 302 32 216 32
Cuts at: 3223 3231
Size: 8
Fragments arranged by size:
812 703 594 302 216 191 92 57
32 32 32 32 32 32 32 32
8
HincII GTy'rAC
Cuts at: 0 368 1844 3231
Size: 368 1476 1387
Fragments arranged by size:
1476 1387 368
HindIII A'AGCT_T
Cuts at: 0 1055 1481 1523 2316 3231
Size: 1055 426 42 793 915
Fragments arranged by size:
1055 915 793 426 42
HpaI GTT'AAC
Cuts at: 0 1844 3231
Size: 1844 1387
KpnI G_GTAC'C
Cuts at: 0 276 3231
Size: 276 2955
MfeI C'AATT_G
Cuts at: 0 206 3231
Size: 206 3025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1748 3231
Size: 1748 1483
MscI TGG'CCA
Cuts at: 0 1202 1304 2084 3231
Size: 1202 102 780 1147
Fragments arranged by size:
1202 1147 780 102
MslI CAynn'nnrTG
Cuts at: 0 1171 1360 2194 3231
Size: 1171 189 834 1037
Fragments arranged by size:
1171 1037 834 189
MspA1I CmG'CkG
Cuts at: 0 841 1678 2325 3231
Size: 841 837 647 906
Fragments arranged by size:
906 841 837 647
NcoI C'CATG_G
Cuts at: 0 908 3231
Size: 908 2323
NspI r_CATG'y
Cuts at: 0 942 1050 2771 3231
Size: 942 108 1721 460
Fragments arranged by size:
1721 942 460 108
PasI CC'CwG_GG
Cuts at: 0 3025 3231
Size: 3025 206
PflMI CCAn_nnn'nTGG
Cuts at: 0 3070 3231
Size: 3070 161
PfoI T'CCnGG_A
Cuts at: 0 1634 1982 3231
Size: 1634 348 1249
Fragments arranged by size:
1634 1249 348
PpuMI rG'GwC_Cy
Cuts at: 0 1257 3231
Size: 1257 1974
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2906 2938 3231
Size: 2906 32 293
Fragments arranged by size:
2906 293 32
PstI C_TGCA'G
Cuts at: 0 1224 1472 1762 3107 3231
Size: 1224 248 290 1345 124
Fragments arranged by size:
1345 1224 290 248 124
PvuII CAG'CTG
Cuts at: 0 841 1678 2325 3231
Size: 841 837 647 906
Fragments arranged by size:
906 841 837 647
SacI G_AGCT'C
Cuts at: 0 1293 3231
Size: 1293 1938
SalI G'TCGA_C
Cuts at: 0 366 3231
Size: 366 2865
SanDI GG'GwC_CC
Cuts at: 0 1257 3231
Size: 1257 1974
SapI GCTCTTCn'nnn_
Cuts at: 0 581 3231
Size: 581 2650
SfcI C'TryA_G
Cuts at: 0 1220 1468 1649 1758 1862 2424 3103 3231
Size: 1220 248 181 109 104 562 679 128
Fragments arranged by size:
1220 679 562 248 181 128 109 104
SmlI C'TyrA_G
Cuts at: 0 1388 1940 3231
Size: 1388 552 1291
Fragments arranged by size:
1388 1291 552
SphI G_CATG'C
Cuts at: 0 1050 3231
Size: 1050 2181
SspI AAT'ATT
Cuts at: 0 1323 3231
Size: 1323 1908
StuI AGG'CCT
Cuts at: 0 582 3231
Size: 582 2649
StyI C'CwwG_G
Cuts at: 0 4 277 845 908 962 1832 3231
Size: 4 273 568 63 54 870 1399
Fragments arranged by size:
1399 870 568 273 63 54 4
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 655 1301 3231
Size: 655 646 1930
Fragments arranged by size:
1930 655 646
TatI w'GTAC_w
Cuts at: 0 528 1250 1821 3231
Size: 528 722 571 1410
Fragments arranged by size:
1410 722 571 528
XmnI GAAnn'nnTTC
Cuts at: 0 1113 1427 3231
Size: 1113 314 1804
Fragments arranged by size:
1804 1113 314
Enzymes that do cut:
AarI AccI Acc65I AcuI AleI AloI AlwNI ApoI
AvrII BamHI BanI BanII BbsI BbvCI BcgI BclI
BglII BmrI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrDI BsrGI BssSI BstBI BstXI BstYI BtgI BtsI
ClaI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI
Hin4I HincII HindIII HpaI KpnI MfeI MmeI MscI
MslI MspA1I NcoI NspI PasI PflMI PfoI PpuMI
PsrI PstI PvuII SacI SalI SanDI SapI SfcI
SmlI SphI SspI StuI StyI TaqII TatI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AlfI
ApaI ApaLI AscI AseI AsiSI AvaI BaeI BbeI
BciVI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI
BpmI BsaAI BsaHI BsiEI BsiWI BspEI BspHI BsrBI
BsrFI BssHII BstAPI BstEII BstZ17I Bsu36I BtgZI CspCI
DraI DraIII DrdI EagI EciI EcoNI EcoRI FseI
FspI FspAI HaeII KasI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII
SacII SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI