Restriction map for
pFN21AE2543
(Linear) (Six-base) MAPSORT of: KIEE2543.seq Check: 2157 from: 1 to: 795
KIEE2543.orf
With 182 enzymes: SgfI *
September 30, 2014 10:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 571 795
Size: 49 522 224
Fragments arranged by size:
522 224 49
AfeI AGC'GCT
Cuts at: 0 317 795
Size: 317 478
AflII C'TTAA_G
Cuts at: 0 111 795
Size: 111 684
AseI AT'TA_AT
Cuts at: 0 505 795
Size: 505 290
AvaI C'yCGr_G
Cuts at: 0 92 795
Size: 92 703
BfrBI ATG'CAT
Cuts at: 0 560 795
Size: 560 235
BmrI ACTGGGnnnn_n'
Cuts at: 0 238 795
Size: 238 557
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 139 795
Size: 139 656
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 334 795
Size: 324 10 461
Fragments arranged by size:
461 324 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 795
Size: 82 713
BsiEI CG_ry'CG
Cuts at: 0 11 15 21 795
Size: 11 4 6 774
Fragments arranged by size:
774 11 6 4
BsmI GAATG_Cn'
Cuts at: 0 474 795
Size: 474 321
BsrBI CCG'CTC
Cuts at: 0 23 795
Size: 23 772
BsrDI GCAATG_nn'
Cuts at: 0 560 795
Size: 560 235
BstAPI GCAn_nnn'nTGC
Cuts at: 0 562 795
Size: 562 233
BstYI r'GATC_y
Cuts at: 0 84 795
Size: 84 711
BtgI C'CryG_G
Cuts at: 0 15 795
Size: 15 780
ClaI AT'CG_AT
Cuts at: 0 647 795
Size: 647 148
DraI TTT'AAA
Cuts at: 0 783 795
Size: 783 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 547 795
Size: 547 248
EaeI y'GGCC_r
Cuts at: 0 12 18 134 210 795
Size: 12 6 116 76 585
Fragments arranged by size:
585 116 76 12 6
EagI C'GGCC_G
Cuts at: 0 12 18 795
Size: 12 6 777
Fragments arranged by size:
777 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 66 795
Size: 66 729
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 324 334 571 795
Size: 49 275 10 237 224
Fragments arranged by size:
275 237 224 49 10
EcoO109I rG'GnC_Cy
Cuts at: 0 675 795
Size: 675 120
EcoRV GAT'ATC
Cuts at: 0 249 325 795
Size: 249 76 470
Fragments arranged by size:
470 249 76
HaeII r_GCGC'y
Cuts at: 0 319 795
Size: 319 476
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 314 346 795
Size: 314 32 449
Fragments arranged by size:
449 314 32
HindIII A'AGCT_T
Cuts at: 0 193 795
Size: 193 602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 795
Size: 495 300
MscI TGG'CCA
Cuts at: 0 136 212 795
Size: 136 76 583
Fragments arranged by size:
583 136 76
MspA1I CmG'CkG
Cuts at: 0 17 62 81 795
Size: 17 45 19 714
Fragments arranged by size:
714 45 19 17
NotI GC'GGCC_GC
Cuts at: 0 18 795
Size: 18 777
NsiI A_TGCA'T
Cuts at: 0 562 795
Size: 562 233
NspI r_CATG'y
Cuts at: 0 560 795
Size: 560 235
PflMI CCAn_nnn'nTGG
Cuts at: 0 132 795
Size: 132 663
PvuI CG_AT'CG
Cuts at: 0 11 795
Size: 11 784
SacII CC_GC'GG
Cuts at: 0 18 795
Size: 18 777
SmlI C'TyrA_G
Cuts at: 0 111 795
Size: 111 684
SphI G_CATG'C
Cuts at: 0 560 795
Size: 560 235
SspI AAT'ATT
Cuts at: 0 767 788 795
Size: 767 21 7
Fragments arranged by size:
767 21 7
Enzymes that do cut:
AcuI AfeI AflII AseI AvaI BfrBI BmrI BplI
BpmI BseRI BsiEI BsmI BsrBI BsrDI BstAPI BstYI
BtgI ClaI DraI DrdI EaeI EagI EciI Eco57MI
EcoO109I EcoRV HaeII Hin4I HindIII MmeI MscI MspA1I
NotI NsiI NspI PflMI PvuI SacII SmlI SphI
SspI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AsiSI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiHKAI BsiWI BsmBI Bsp1286I
BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI BtsI CspCI DraIII
EarI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI
HincII HpaI KasI KpnI MfeI MluI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NruI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI