Restriction map for
pFN21AE2539
(Linear) (Six-base) MAPSORT of: KIEE2539.seq Check: 2502 from: 1 to: 2040
KIEE2539.orf
With 182 enzymes: SgfI *
September 19, 2014 17:03 ..
AccI GT'mk_AC
Cuts at: 0 1772 2040
Size: 1772 268
AclI AA'CG_TT
Cuts at: 0 1981 2040
Size: 1981 59
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 945 1903 2040
Size: 57 888 958 137
Fragments arranged by size:
958 888 137 57
AflIII A'CryG_T
Cuts at: 0 458 2040
Size: 458 1582
AhdI GACnn_n'nnGTC
Cuts at: 0 1545 2040
Size: 1545 495
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 672 704 2040
Size: 672 32 1336
Fragments arranged by size:
1336 672 32
ApaI G_GGCC'C
Cuts at: 0 641 2040
Size: 641 1399
ApaLI G'TGCA_C
Cuts at: 0 979 2040
Size: 979 1061
ApoI r'AATT_y
Cuts at: 0 2012 2040
Size: 2012 28
AvaI C'yCGr_G
Cuts at: 0 675 1480 2040
Size: 675 805 560
Fragments arranged by size:
805 675 560
BanII G_rGCy'C
Cuts at: 0 641 1470 1840 2040
Size: 641 829 370 200
Fragments arranged by size:
829 641 370 200
BbsI GAAGACnn'nnnn_
Cuts at: 0 882 2006 2040
Size: 882 1124 34
Fragments arranged by size:
1124 882 34
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 319 353 715 749 2040
Size: 319 34 362 34 1291
Fragments arranged by size:
1291 362 319 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 81 1009 2040
Size: 81 928 1031
Fragments arranged by size:
1031 928 81
BglI GCCn_nnn'nGGC
Cuts at: 0 645 2040
Size: 645 1395
BglII A'GATC_T
Cuts at: 0 1820 2040
Size: 1820 220
Bme1580I G_kGCm'C
Cuts at: 0 641 983 1967 2040
Size: 641 342 984 73
Fragments arranged by size:
984 641 342 73
BmrI ACTGGGnnnn_n'
Cuts at: 0 1192 2040
Size: 1192 848
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1056 1452 1477 2040
Size: 1056 396 25 563
Fragments arranged by size:
1056 563 396 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 883 1733 2040
Size: 393 490 850 307
Fragments arranged by size:
850 490 393 307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 652 2040
Size: 643 9 1388
Fragments arranged by size:
1388 643 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 762 1128 1968 2040
Size: 762 366 840 72
Fragments arranged by size:
840 762 366 72
BsaAI yAC'GTr
Cuts at: 0 461 612 2040
Size: 461 151 1428
Fragments arranged by size:
1428 461 151
BsaWI w'CCGG_w
Cuts at: 0 121 2040
Size: 121 1919
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 536 566 2040
Size: 536 30 1474
Fragments arranged by size:
1474 536 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 560 1032 1534 2040
Size: 560 472 502 506
Fragments arranged by size:
560 506 502 472
BseYI C'CCAG_C
Cuts at: 0 8 270 584 736 2040
Size: 8 262 314 152 1304
Fragments arranged by size:
1304 314 262 152 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1380 1902 2040
Size: 1380 522 138
Fragments arranged by size:
1380 522 138
BsiHKAI G_wGCw'C
Cuts at: 0 192 983 2040
Size: 192 791 1057
Fragments arranged by size:
1057 791 192
BsmBI CGTCTCn'nnnn_
Cuts at: 0 542 2040
Size: 542 1498
Bsp1286I G_dGCh'C
Cuts at: 0 192 641 983 1470 1840 1967 2040
Size: 192 449 342 487 370 127 73
Fragments arranged by size:
487 449 370 342 192 127 73
BspEI T'CCGG_A
Cuts at: 0 121 2040
Size: 121 1919
BsrFI r'CCGG_y
Cuts at: 0 494 519 972 2040
Size: 494 25 453 1068
Fragments arranged by size:
1068 494 453 25
BsrGI T'GTAC_A
Cuts at: 0 1931 2040
Size: 1931 109
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1962 2040
Size: 1962 78
BstEII G'GTnAC_C
Cuts at: 0 36 1269 2040
Size: 36 1233 771
Fragments arranged by size:
1233 771 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1575 1739 2040
Size: 1575 164 301
Fragments arranged by size:
1575 301 164
BstYI r'GATC_y
Cuts at: 0 1820 2040
Size: 1820 220
BstZ17I GTA'TAC
Cuts at: 0 1773 2040
Size: 1773 267
Bsu36I CC'TnA_GG
Cuts at: 0 1517 2040
Size: 1517 523
BtgI C'CryG_G
Cuts at: 0 41 1274 1859 2040
Size: 41 1233 585 181
Fragments arranged by size:
1233 585 181 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 322 2040
Size: 322 1718
BtsI GCAGTG_nn'
Cuts at: 0 1241 1967 2040
Size: 1241 726 73
Fragments arranged by size:
1241 726 73
EaeI y'GGCC_r
Cuts at: 0 245 1277 1565 2040
Size: 245 1032 288 475
Fragments arranged by size:
1032 475 288 245
EarI CTCTTCn'nnn_
Cuts at: 0 822 1095 2040
Size: 822 273 945
Fragments arranged by size:
945 822 273
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 745 1243 2040
Size: 745 498 797
Fragments arranged by size:
797 745 498
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 393 883 945 1733 1903 2040
Size: 57 336 490 62 788 170 137
Fragments arranged by size:
788 490 336 170 137 62 57
EcoO109I rG'GnC_Cy
Cuts at: 0 1146 2040
Size: 1146 894
EcoRI G'AATT_C
Cuts at: 0 2012 2040
Size: 2012 28
EcoRV GAT'ATC
Cuts at: 0 1686 2040
Size: 1686 354
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 975 2040
Size: 975 1065
HaeII r_GCGC'y
Cuts at: 0 823 2040
Size: 823 1217
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 100 132 1315 1347 1531 1563 1780 1812
Size: 100 32 1183 32 184 32 217 32
Cuts at: 1812 2040
Size: 228
Fragments arranged by size:
1183 228 217 184 100 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 522 1079 2040
Size: 522 557 961
Fragments arranged by size:
961 557 522
MscI TGG'CCA
Cuts at: 0 1567 2040
Size: 1567 473
MslI CAynn'nnrTG
Cuts at: 0 470 1108 1784 1939 2040
Size: 470 638 676 155 101
Fragments arranged by size:
676 638 470 155 101
MspA1I CmG'CkG
Cuts at: 0 8 1065 1563 2040
Size: 8 1057 498 477
Fragments arranged by size:
1057 498 477 8
NaeI GCC'GGC
Cuts at: 0 521 974 2040
Size: 521 453 1066
Fragments arranged by size:
1066 521 453
NcoI C'CATG_G
Cuts at: 0 41 2040
Size: 41 1999
NgoMIV G'CCGG_C
Cuts at: 0 519 972 2040
Size: 519 453 1068
Fragments arranged by size:
1068 519 453
NspI r_CATG'y
Cuts at: 0 1960 2040
Size: 1960 80
PasI CC'CwG_GG
Cuts at: 0 1194 1849 2040
Size: 1194 655 191
Fragments arranged by size:
1194 655 191
PflMI CCAn_nnn'nTGG
Cuts at: 0 302 2040
Size: 302 1738
PmlI CAC'GTG
Cuts at: 0 612 2040
Size: 612 1428
PpuMI rG'GwC_Cy
Cuts at: 0 1146 2040
Size: 1146 894
PspOMI G'GGCC_C
Cuts at: 0 637 2040
Size: 637 1403
PvuII CAG'CTG
Cuts at: 0 8 1563 2040
Size: 8 1555 477
Fragments arranged by size:
1555 477 8
ScaI AGT'ACT
Cuts at: 0 1492 1582 2040
Size: 1492 90 458
Fragments arranged by size:
1492 458 90
SmaI CCC'GGG
Cuts at: 0 1482 2040
Size: 1482 558
SmlI C'TyrA_G
Cuts at: 0 658 667 2040
Size: 658 9 1373
Fragments arranged by size:
1373 658 9
SphI G_CATG'C
Cuts at: 0 1960 2040
Size: 1960 80
StyI C'CwwG_G
Cuts at: 0 41 1259 2040
Size: 41 1218 781
Fragments arranged by size:
1218 781 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 739 2040
Size: 739 1301
TatI w'GTAC_w
Cuts at: 0 1490 1580 1931 2040
Size: 1490 90 351 109
Fragments arranged by size:
1490 351 109 90
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 299 1062 1629 2040
Size: 299 763 567 411
Fragments arranged by size:
763 567 411 299
XmaI C'CCGG_G
Cuts at: 0 1480 2040
Size: 1480 560
Enzymes that do cut:
AccI AclI AcuI AflIII AhdI AloI ApaI ApaLI
ApoI AvaI BanII BbsI BcgI BciVI BglI BglII
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BsrFI BsrGI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PmlI
PpuMI PspOMI PvuII ScaI SmaI SmlI SphI StyI
TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AleI AlfI
AlwNI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BbvCI BclI BfrBI BlpI BmgBI BmtI Bpu10I
BsaBI BsaHI BsiEI BsiWI BsmI BspHI BspMI BsrBI
BsrDI BssHII BssSI BstBI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoNI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI NarI NdeI
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfcI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
StuI SwaI TstI Tth111I XbaI XhoI XmnI ZraI