Restriction map for
pFN21AE2537
(Linear) (Six-base) MAPSORT of: KIEE2537.seq Check: 9708 from: 1 to: 855
KIEE2537.orf
With 182 enzymes: SgfI *
July 25, 2014 11:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 171 826 855
Size: 171 655 29
Fragments arranged by size:
655 171 29
AatII G_ACGT'C
Cuts at: 0 119 855
Size: 119 736
AccI GT'mk_AC
Cuts at: 0 848 855
Size: 848 7
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 775 855
Size: 319 456 80
Fragments arranged by size:
456 319 80
AflII C'TTAA_G
Cuts at: 0 592 855
Size: 592 263
ApoI r'AATT_y
Cuts at: 0 409 855
Size: 409 446
AvaI C'yCGr_G
Cuts at: 0 64 277 855
Size: 64 213 578
Fragments arranged by size:
578 213 64
BanI G'GyrC_C
Cuts at: 0 282 855
Size: 282 573
BanII G_rGCy'C
Cuts at: 0 11 855
Size: 11 844
BbeI G_GCGC'C
Cuts at: 0 286 855
Size: 286 569
BbsI GAAGACnn'nnnn_
Cuts at: 0 216 855
Size: 216 639
BciVI GTATCCnnnnn_n'
Cuts at: 0 734 855
Size: 734 121
Bme1580I G_kGCm'C
Cuts at: 0 695 855
Size: 695 160
BmgBI CAC'GTC
Cuts at: 0 237 696 855
Size: 237 459 159
Fragments arranged by size:
459 237 159
BsaAI yAC'GTr
Cuts at: 0 150 855
Size: 150 705
BsaHI Gr'CG_yC
Cuts at: 0 116 283 855
Size: 116 167 572
Fragments arranged by size:
572 167 116
BsaWI w'CCGG_w
Cuts at: 0 213 855
Size: 213 642
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 268 855
Size: 121 147 587
Fragments arranged by size:
587 147 121
BseYI C'CCAG_C
Cuts at: 0 159 171 541 855
Size: 159 12 370 314
Fragments arranged by size:
370 314 159 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 688 855
Size: 563 125 167
Fragments arranged by size:
563 167 125
BsiEI CG_ry'CG
Cuts at: 0 33 200 855
Size: 33 167 655
Fragments arranged by size:
655 167 33
BsmBI CGTCTCn'nnnn_
Cuts at: 0 250 855
Size: 250 605
Bsp1286I G_dGCh'C
Cuts at: 0 11 695 855
Size: 11 684 160
Fragments arranged by size:
684 160 11
BspEI T'CCGG_A
Cuts at: 0 213 855
Size: 213 642
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 171 826 855
Size: 171 655 29
Fragments arranged by size:
655 171 29
BsrFI r'CCGG_y
Cuts at: 0 195 855
Size: 195 660
BstAPI GCAn_nnn'nTGC
Cuts at: 0 699 855
Size: 699 156
BtgI C'CryG_G
Cuts at: 0 47 242 855
Size: 47 195 613
Fragments arranged by size:
613 195 47
DraIII CAC_nnn'GTG
Cuts at: 0 48 855
Size: 48 807
DrdI GACnn_nn'nnGTC
Cuts at: 0 484 855
Size: 484 371
EaeI y'GGCC_r
Cuts at: 0 2 12 30 75 197 239 855
Size: 2 10 18 45 122 42 616
Fragments arranged by size:
616 122 45 42 18 10 2
EagI C'GGCC_G
Cuts at: 0 30 197 855
Size: 30 167 658
Fragments arranged by size:
658 167 30
EarI CTCTTCn'nnn_
Cuts at: 0 517 713 855
Size: 517 196 142
Fragments arranged by size:
517 196 142
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 398 855
Size: 398 457
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 775 855
Size: 319 456 80
Fragments arranged by size:
456 319 80
EcoO109I rG'GnC_Cy
Cuts at: 0 588 855
Size: 588 267
HaeII r_GCGC'y
Cuts at: 0 61 98 286 855
Size: 61 37 188 569
Fragments arranged by size:
569 188 61 37
HincII GTy'rAC
Cuts at: 0 306 855
Size: 306 549
KasI G'GCGC_C
Cuts at: 0 282 855
Size: 282 573
MfeI C'AATT_G
Cuts at: 0 560 855
Size: 560 295
NaeI GCC'GGC
Cuts at: 0 197 855
Size: 197 658
NarI GG'CG_CC
Cuts at: 0 283 855
Size: 283 572
NdeI CA'TA_TG
Cuts at: 0 764 855
Size: 764 91
NgoMIV G'CCGG_C
Cuts at: 0 195 855
Size: 195 660
NspI r_CATG'y
Cuts at: 0 786 855
Size: 786 69
PacI TTA_AT'TAA
Cuts at: 0 836 855
Size: 836 19
PflMI CCAn_nnn'nTGG
Cuts at: 0 48 855
Size: 48 807
PstI C_TGCA'G
Cuts at: 0 182 535 855
Size: 182 353 320
Fragments arranged by size:
353 320 182
SfcI C'TryA_G
Cuts at: 0 178 531 855
Size: 178 353 324
Fragments arranged by size:
353 324 178
SfoI GGC'GCC
Cuts at: 0 284 855
Size: 284 571
SmaI CCC'GGG
Cuts at: 0 279 855
Size: 279 576
SmlI C'TyrA_G
Cuts at: 0 592 855
Size: 592 263
StyI C'CwwG_G
Cuts at: 0 98 855
Size: 98 757
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 55 855
Size: 55 800
Tth111I GACn'n_nGTC
Cuts at: 0 220 855
Size: 220 635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 45 498 855
Size: 45 453 357
Fragments arranged by size:
453 357 45
XmaI C'CCGG_G
Cuts at: 0 277 855
Size: 277 578
XmnI GAAnn'nnTTC
Cuts at: 0 332 526 855
Size: 332 194 329
Fragments arranged by size:
332 329 194
ZraI GAC'GTC
Cuts at: 0 117 855
Size: 117 738
Enzymes that do cut:
AarI AatII AccI AcuI AflII ApoI AvaI BanI
BanII BbeI BbsI BciVI Bme1580I BmgBI BsaAI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspEI
BspMI BsrFI BstAPI BtgI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I HaeII HincII KasI MfeI
NaeI NarI NdeI NgoMIV NspI PacI PflMI PstI
SfcI SfoI SmaI SmlI StyI TaqII Tth111I XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflIII AgeI AhdI AleI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BclI BfrBI BglI BglII
BlpI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaXI BsiHKAI BsiWI BsmI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII
HpaI KpnI MluI MmeI MscI MslI MspA1I NcoI
NheI NotI NruI NsiI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI XbaI
XhoI