Restriction map for
pFN21AE2531
(Linear) (Six-base) MAPSORT of: KIEE2531.seq Check: 1965 from: 1 to: 2103
KIEE2531.orf
With 182 enzymes: SgfI *
July 15, 2014 16:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1874 2103
Size: 1874 229
AclI AA'CG_TT
Cuts at: 0 288 2103
Size: 288 1815
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 602 2103
Size: 362 240 1501
Fragments arranged by size:
1501 362 240
AflIII A'CryG_T
Cuts at: 0 304 2103
Size: 304 1799
AleI CACnn'nnGTG
Cuts at: 0 973 2103
Size: 973 1130
AlwNI CAG_nnn'CTG
Cuts at: 0 368 1020 2103
Size: 368 652 1083
Fragments arranged by size:
1083 652 368
ApoI r'AATT_y
Cuts at: 0 183 536 1305 2073 2103
Size: 183 353 769 768 30
Fragments arranged by size:
769 768 353 183 30
AvaI C'yCGr_G
Cuts at: 0 1946 2103
Size: 1946 157
BanI G'GyrC_C
Cuts at: 0 1277 1886 2103
Size: 1277 609 217
Fragments arranged by size:
1277 609 217
BanII G_rGCy'C
Cuts at: 0 2062 2103
Size: 2062 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 368 1763 1907 2103
Size: 368 1395 144 196
Fragments arranged by size:
1395 368 196 144
BclI T'GATC_A
Cuts at: 0 891 1056 1455 2003 2103
Size: 891 165 399 548 100
Fragments arranged by size:
891 548 399 165 100
BfrBI ATG'CAT
Cuts at: 0 280 936 2103
Size: 280 656 1167
Fragments arranged by size:
1167 656 280
Bme1580I G_kGCm'C
Cuts at: 0 174 1282 2103
Size: 174 1108 821
Fragments arranged by size:
1108 821 174
BmrI ACTGGGnnnn_n'
Cuts at: 0 1154 1243 2103
Size: 1154 89 860
Fragments arranged by size:
1154 860 89
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 289 2103
Size: 289 1814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 350 1463 2103
Size: 53 297 1113 640
Fragments arranged by size:
1113 640 297 53
Bpu10I CC'TnA_GC
Cuts at: 0 260 2103
Size: 260 1843
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 795 2103
Size: 795 1308
BsaBI GATnn'nnATC
Cuts at: 0 1511 2002 2103
Size: 1511 491 101
Fragments arranged by size:
1511 491 101
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1718 1748 2103
Size: 1718 30 355
Fragments arranged by size:
1718 355 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 756 2103
Size: 756 1347
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1839 1902 2103
Size: 1839 63 201
Fragments arranged by size:
1839 201 63
BsiHKAI G_wGCw'C
Cuts at: 0 557 2062 2103
Size: 557 1505 41
Fragments arranged by size:
1505 557 41
BsmI GAATG_Cn'
Cuts at: 0 261 643 2103
Size: 261 382 1460
Fragments arranged by size:
1460 382 261
Bsp1286I G_dGCh'C
Cuts at: 0 174 557 1282 2062 2103
Size: 174 383 725 780 41
Fragments arranged by size:
780 725 383 174 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1874 2103
Size: 1874 229
BsrDI GCAATG_nn'
Cuts at: 0 1961 2103
Size: 1961 142
BssSI C'ACGA_G
Cuts at: 0 46 454 2103
Size: 46 408 1649
Fragments arranged by size:
1649 408 46
BstAPI GCAn_nnn'nTGC
Cuts at: 0 368 2103
Size: 368 1735
BstXI CCAn_nnnn'nTGG
Cuts at: 0 922 1478 2103
Size: 922 556 625
Fragments arranged by size:
922 625 556
BstYI r'GATC_y
Cuts at: 0 138 2103
Size: 138 1965
BtsI GCAGTG_nn'
Cuts at: 0 369 980 1053 2103
Size: 369 611 73 1050
Fragments arranged by size:
1050 611 369 73
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 345 380 1447 1482 2103
Size: 345 35 1067 35 621
Fragments arranged by size:
1067 621 345 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1237 2103
Size: 1237 866
DrdI GACnn_nn'nnGTC
Cuts at: 0 1957 2103
Size: 1957 146
EaeI y'GGCC_r
Cuts at: 0 705 1468 2103
Size: 705 763 635
Fragments arranged by size:
763 705 635
EarI CTCTTCn'nnn_
Cuts at: 0 47 774 1368 1650 2103
Size: 47 727 594 282 453
Fragments arranged by size:
727 594 453 282 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 107 2103
Size: 107 1996
EcoICRI GAG'CTC
Cuts at: 0 2060 2103
Size: 2060 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 350 362 602 1463 2103
Size: 53 297 12 240 861 640
Fragments arranged by size:
861 640 297 240 53 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1004 2103
Size: 1004 1099
EcoRI G'AATT_C
Cuts at: 0 536 2103
Size: 536 1567
EcoRV GAT'ATC
Cuts at: 0 117 2103
Size: 117 1986
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 568 600 934 966 1807 1839 2049 2081
Size: 568 32 334 32 841 32 210 32
Cuts at: 2081 2103
Size: 22
Fragments arranged by size:
841 568 334 210 32 32 32 32
22
MfeI C'AATT_G
Cuts at: 0 668 2103
Size: 668 1435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 923 2103
Size: 923 1180
MscI TGG'CCA
Cuts at: 0 1470 2103
Size: 1470 633
MslI CAynn'nnrTG
Cuts at: 0 973 2103
Size: 973 1130
MspA1I CmG'CkG
Cuts at: 0 1545 2103
Size: 1545 558
NdeI CA'TA_TG
Cuts at: 0 228 2103
Size: 228 1875
NsiI A_TGCA'T
Cuts at: 0 282 938 2103
Size: 282 656 1165
Fragments arranged by size:
1165 656 282
NspI r_CATG'y
Cuts at: 0 308 2098 2103
Size: 308 1790 5
Fragments arranged by size:
1790 308 5
PasI CC'CwG_GG
Cuts at: 0 875 2103
Size: 875 1228
PciI A'CATG_T
Cuts at: 0 304 2103
Size: 304 1799
PflMI CCAn_nnn'nTGG
Cuts at: 0 471 714 2103
Size: 471 243 1389
Fragments arranged by size:
1389 471 243
PshAI GACnn'nnGTC
Cuts at: 0 670 2103
Size: 670 1433
PsiI TTA'TAA
Cuts at: 0 1687 1720 2103
Size: 1687 33 383
Fragments arranged by size:
1687 383 33
PstI C_TGCA'G
Cuts at: 0 1092 2103
Size: 1092 1011
SacI G_AGCT'C
Cuts at: 0 2062 2103
Size: 2062 41
SapI GCTCTTCn'nnn_
Cuts at: 0 47 2103
Size: 47 2056
SfcI C'TryA_G
Cuts at: 0 570 630 1088 1556 2103
Size: 570 60 458 468 547
Fragments arranged by size:
570 547 468 458 60
SmlI C'TyrA_G
Cuts at: 0 774 2103
Size: 774 1329
SphI G_CATG'C
Cuts at: 0 2098 2103
Size: 2098 5
StuI AGG'CCT
Cuts at: 0 1605 2103
Size: 1605 498
StyI C'CwwG_G
Cuts at: 0 322 915 1477 2103
Size: 322 593 562 626
Fragments arranged by size:
626 593 562 322
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 449 1225 2103
Size: 449 776 878
Fragments arranged by size:
878 776 449
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 799 831 1261 1293 1891 1923 2103
Size: 799 32 430 32 598 32 180
Fragments arranged by size:
799 598 430 180 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 514 1849 2103
Size: 514 1335 254
Fragments arranged by size:
1335 514 254
XbaI T'CTAG_A
Cuts at: 0 234 2103
Size: 234 1869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 975 1438 2103
Size: 975 463 665
Fragments arranged by size:
975 665 463
Enzymes that do cut:
AarI AclI AcuI AflIII AleI AlwNI ApoI AvaI
BanI BanII BbsI BclI BfrBI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaBI BsaXI BseRI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BssSI BstAPI BstXI BstYI
BtsI CspCI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoRI EcoRV Hin4I MfeI MmeI MscI
MslI MspA1I NdeI NsiI NspI PasI PciI PflMI
PshAI PsiI PstI SacI SapI SfcI SmlI SphI
StuI StyI TaqII TstI Tth111I XbaI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BglI BglII BlpI
BmgBI BmtI BsaI BsaAI BsaHI BsaWI BseYI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI DraI
EagI EcoO109I FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MluI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI PfoI PmeI PmlI
PpiI PpuMI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TatI XhoI XmaI XmnI ZraI