(Linear) (Six-base) MAPSORT of: KIEE2529.seq  Check: 6134  from: 1  to: 576



KIEE2529.orf

 With 182 enzymes: SgfI * 

                        June 11, 2014 14:42  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     264     576 
   Size:        264     312
 
AseI AT'TA_AT

Cuts at:      0     325     576 
   Size:        325     251
 
AvaI C'yCGr_G

Cuts at:      0      47     576 
   Size:         47     529
 
BanI G'GyrC_C

Cuts at:      0      43      79     576 
   Size:         43      36     497

  Fragments arranged by size: 

                 497      43      36
 
BbeI G_GCGC'C

Cuts at:      0      47      83     576 
   Size:         47      36     493

  Fragments arranged by size: 

                 493      47      36
 
Bme1580I G_kGCm'C

Cuts at:      0     518     576 
   Size:        518      58
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     545     576 
   Size:        545      31
 
BsaHI Gr'CG_yC

Cuts at:      0      44      80     140     576 
   Size:         44      36      60     436

  Fragments arranged by size: 

                 436      60      44      36
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     250     576 
   Size:        250     326
 
BseYI C'CCAG_C

Cuts at:      0      21     190     576 
   Size:         21     169     386

  Fragments arranged by size: 

                 386     169      21
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     213     576 
   Size:        213     363
 
Bsp1286I G_dGCh'C

Cuts at:      0     518     576 
   Size:        518      58
 
BsrDI GCAATG_nn'

Cuts at:      0     304     576 
   Size:        304     272
 
BssHII G'CGCG_C

Cuts at:      0     160     576 
   Size:        160     416
 
BstBI TT'CG_AA

Cuts at:      0     537     576 
   Size:        537      39
 
BtgI C'CryG_G

Cuts at:      0     174     200     576 
   Size:        174      26     376

  Fragments arranged by size: 

                 376     174      26
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     347     382     576 
   Size:        347      35     194

  Fragments arranged by size: 

                 347     194      35
 
DraI TTT'AAA

Cuts at:      0     402     555     576 
   Size:        402     153      21

  Fragments arranged by size: 

                 402     153      21
 
EaeI y'GGCC_r

Cuts at:      0       2     177     576 
   Size:          2     175     399

  Fragments arranged by size: 

                 399     175       2
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     545     576 
   Size:        545      31
 
EcoO109I rG'GnC_Cy

Cuts at:      0     186     576 
   Size:        186     390
 
HaeII r_GCGC'y

Cuts at:      0      47      83     576 
   Size:         47      36     493

  Fragments arranged by size: 

                 493      47      36
 
KasI G'GCGC_C

Cuts at:      0      43      79     576 
   Size:         43      36     497

  Fragments arranged by size: 

                 497      43      36
 
MslI CAynn'nnrTG

Cuts at:      0     352     576 
   Size:        352     224
 
MspA1I CmG'CkG

Cuts at:      0     176     227     565     576 
   Size:        176      51     338      11

  Fragments arranged by size: 

                 338     176      51      11
 
NarI GG'CG_CC

Cuts at:      0      44      80     576 
   Size:         44      36     496

  Fragments arranged by size: 

                 496      44      36
 
NspI r_CATG'y

Cuts at:      0     375     576 
   Size:        375     201
 
PpuMI rG'GwC_Cy

Cuts at:      0     186     576 
   Size:        186     390
 
PvuII CAG'CTG

Cuts at:      0     227     565     576 
   Size:        227     338      11

  Fragments arranged by size: 

                 338     227      11
 
SacII CC_GC'GG

Cuts at:      0     177     576 
   Size:        177     399
 
SfoI GGC'GCC

Cuts at:      0      45      81     576 
   Size:         45      36     495

  Fragments arranged by size: 

                 495      45      36
 
SmaI CCC'GGG

Cuts at:      0      49     576 
   Size:         49     527
 
StyI C'CwwG_G

Cuts at:      0     332     576 
   Size:        332     244
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      72     576 
   Size:         72     504
 
XmaI C'CCGG_G

Cuts at:      0      47     576 
   Size:         47     529
 

 Enzymes that do cut:

    AlwNI     AseI     AvaI     BanI     BbeI Bme1580I     BpmI    BsaHI
    BseRI    BseYI     BsgI Bsp1286I    BsrDI   BssHII    BstBI     BtgI
    CspCI     DraI     EaeI  Eco57MI EcoO109I    HaeII     KasI     MslI
   MspA1I     NarI     NspI    PpuMI    PvuII    SacII     SfoI     SmaI
     StyI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI    AsiSI    AvrII     BaeI    BamHI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI    BssSI   BstAPI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmnI
     ZraI