Restriction map for
pFN21AE2524
(Linear) (Six-base) MAPSORT of: KIEE2524.seq Check: 8717 from: 1 to: 1371
KIEE2524.orf
With 182 enzymes: SgfI *
June 11, 2014 14:42 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 1205 1371
Size: 668 537 166
Fragments arranged by size:
668 537 166
AhdI GACnn_n'nnGTC
Cuts at: 0 129 1371
Size: 129 1242
AleI CACnn'nnGTG
Cuts at: 0 338 1040 1371
Size: 338 702 331
Fragments arranged by size:
702 338 331
AlwNI CAG_nnn'CTG
Cuts at: 0 975 1371
Size: 975 396
ApoI r'AATT_y
Cuts at: 0 10 508 790 957 1300 1371
Size: 10 498 282 167 343 71
Fragments arranged by size:
498 343 282 167 71 10
AvaI C'yCGr_G
Cuts at: 0 46 127 627 1371
Size: 46 81 500 744
Fragments arranged by size:
744 500 81 46
BamHI G'GATC_C
Cuts at: 0 481 1371
Size: 481 890
BanI G'GyrC_C
Cuts at: 0 87 301 1371
Size: 87 214 1070
Fragments arranged by size:
1070 214 87
BanII G_rGCy'C
Cuts at: 0 38 254 1371
Size: 38 216 1117
Fragments arranged by size:
1117 216 38
BbeI G_GCGC'C
Cuts at: 0 91 305 1371
Size: 91 214 1066
Fragments arranged by size:
1066 214 91
BbvCI CC'TCA_GC
Cuts at: 0 521 1371
Size: 521 850
BciVI GTATCCnnnnn_n'
Cuts at: 0 1115 1371
Size: 1115 256
BglI GCCn_nnn'nGGC
Cuts at: 0 48 1371
Size: 48 1323
BlpI GC'TnA_GC
Cuts at: 0 58 1371
Size: 58 1313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 634 878 1371
Size: 556 78 244 493
Fragments arranged by size:
556 493 244 78
Bpu10I CC'TnA_GC
Cuts at: 0 358 521 1371
Size: 358 163 850
Fragments arranged by size:
850 358 163
BsaHI Gr'CG_yC
Cuts at: 0 88 113 302 1371
Size: 88 25 189 1069
Fragments arranged by size:
1069 189 88 25
BsaWI w'CCGG_w
Cuts at: 0 1097 1371
Size: 1097 274
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 876 906 1109 1139 1371
Size: 876 30 203 30 232
Fragments arranged by size:
876 232 203 30 30
BseYI C'CCAG_C
Cuts at: 0 141 209 1371
Size: 141 68 1162
Fragments arranged by size:
1162 141 68
BsiHKAI G_wGCw'C
Cuts at: 0 382 431 1371
Size: 382 49 940
Fragments arranged by size:
940 382 49
BsmI GAATG_Cn'
Cuts at: 0 1153 1371
Size: 1153 218
Bsp1286I G_dGCh'C
Cuts at: 0 38 254 382 431 1371
Size: 38 216 128 49 940
Fragments arranged by size:
940 216 128 49 38
BspEI T'CCGG_A
Cuts at: 0 1097 1371
Size: 1097 274
BsrBI CCG'CTC
Cuts at: 0 45 162 1371
Size: 45 117 1209
Fragments arranged by size:
1209 117 45
BsrDI GCAATG_nn'
Cuts at: 0 871 1371
Size: 871 500
BsrFI r'CCGG_y
Cuts at: 0 632 1371
Size: 632 739
BsrGI T'GTAC_A
Cuts at: 0 1275 1371
Size: 1275 96
BstBI TT'CG_AA
Cuts at: 0 992 1371
Size: 992 379
BstXI CCAn_nnnn'nTGG
Cuts at: 0 141 534 1371
Size: 141 393 837
Fragments arranged by size:
837 393 141
BstYI r'GATC_y
Cuts at: 0 481 1371
Size: 481 890
BtgI C'CryG_G
Cuts at: 0 527 1371
Size: 527 844
EaeI y'GGCC_r
Cuts at: 0 147 1371
Size: 147 1224
EarI CTCTTCn'nnn_
Cuts at: 0 1348 1371
Size: 1348 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 46 167 1371
Size: 46 121 1204
Fragments arranged by size:
1204 121 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 634 668 878 1205 1371
Size: 556 78 34 210 327 166
Fragments arranged by size:
556 327 210 166 78 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 319 1371
Size: 319 1052
EcoO109I rG'GnC_Cy
Cuts at: 0 269 312 464 1371
Size: 269 43 152 907
Fragments arranged by size:
907 269 152 43
EcoRI G'AATT_C
Cuts at: 0 957 1371
Size: 957 414
HaeII r_GCGC'y
Cuts at: 0 91 305 1371
Size: 91 214 1066
Fragments arranged by size:
1066 214 91
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 1079 1111 1371
Size: 115 32 932 32 260
Fragments arranged by size:
932 260 115 32 32
HindIII A'AGCT_T
Cuts at: 0 1360 1371
Size: 1360 11
KasI G'GCGC_C
Cuts at: 0 87 301 1371
Size: 87 214 1070
Fragments arranged by size:
1070 214 87
MslI CAynn'nnrTG
Cuts at: 0 338 1040 1371
Size: 338 702 331
Fragments arranged by size:
702 338 331
MspA1I CmG'CkG
Cuts at: 0 141 186 192 198 204 1371
Size: 141 45 6 6 6 1167
Fragments arranged by size:
1167 141 45 6 6 6
NaeI GCC'GGC
Cuts at: 0 634 1371
Size: 634 737
NarI GG'CG_CC
Cuts at: 0 88 302 1371
Size: 88 214 1069
Fragments arranged by size:
1069 214 88
NcoI C'CATG_G
Cuts at: 0 527 1371
Size: 527 844
NgoMIV G'CCGG_C
Cuts at: 0 632 1371
Size: 632 739
NspI r_CATG'y
Cuts at: 0 369 1371
Size: 369 1002
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 313 345 1371
Size: 313 32 1026
Fragments arranged by size:
1026 313 32
PpuMI rG'GwC_Cy
Cuts at: 0 464 1371
Size: 464 907
PshAI GACnn'nnGTC
Cuts at: 0 1266 1371
Size: 1266 105
PsiI TTA'TAA
Cuts at: 0 1201 1371
Size: 1201 170
PspXI vC'TCGA_Gb
Cuts at: 0 127 1371
Size: 127 1244
PstI C_TGCA'G
Cuts at: 0 678 1371
Size: 678 693
PvuII CAG'CTG
Cuts at: 0 141 1371
Size: 141 1230
ScaI AGT'ACT
Cuts at: 0 1182 1371
Size: 1182 189
SfcI C'TryA_G
Cuts at: 0 674 1371
Size: 674 697
SfoI GGC'GCC
Cuts at: 0 89 303 1371
Size: 89 214 1068
Fragments arranged by size:
1068 214 89
SmlI C'TyrA_G
Cuts at: 0 127 1371
Size: 127 1244
StyI C'CwwG_G
Cuts at: 0 405 527 603 848 1371
Size: 405 122 76 245 523
Fragments arranged by size:
523 405 245 122 76
TatI w'GTAC_w
Cuts at: 0 1180 1275 1371
Size: 1180 95 96
Fragments arranged by size:
1180 96 95
XhoI C'TCGA_G
Cuts at: 0 127 1371
Size: 127 1244
Enzymes that do cut:
AcuI AhdI AleI AlwNI ApoI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BglI BlpI BpmI Bpu10I
BsaHI BsaWI BsaXI BseYI BsiHKAI BsmI Bsp1286I BspEI
BsrBI BsrDI BsrFI BsrGI BstBI BstXI BstYI BtgI
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I HindIII KasI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PpiI PpuMI PshAI PsiI PspXI PstI
PvuII ScaI SfcI SfoI SmlI StyI TatI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbsI BcgI BclI BfrBI BglII Bme1580I
BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI
BseRI BsgI BsiEI BsiWI BsmBI BspHI BspMI BssHII
BssSI BstAPI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoRV FalI
FseI FspI FspAI HincII HpaI KpnI MfeI MluI
MmeI MscI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PspOMI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XcmI XmaI XmnI ZraI